NCO-sP(EO-stat-PO) Coatings on Gold Sensors-a QCM Study of Hemocompatibility

Please always quote using this URN: urn:nbn:de:bvb:20-opus-141110
  • The reliability of implantable blood sensors is often hampered by unspecific adsorption of plasma proteins and blood cells. This not only leads to a loss of sensor signal over time, but can also result in undesired host vs. graft reactions. Within this study we evaluated the hemocompatibility of isocyanate conjugated star shaped polytheylene oxide-polypropylene oxide co-polymers NCO-sP(EO-stat-PO) when applied to gold surfaces as an auspicious coating material for gold sputtered blood contacting sensors. Quartz crystal microbalance (QCM)The reliability of implantable blood sensors is often hampered by unspecific adsorption of plasma proteins and blood cells. This not only leads to a loss of sensor signal over time, but can also result in undesired host vs. graft reactions. Within this study we evaluated the hemocompatibility of isocyanate conjugated star shaped polytheylene oxide-polypropylene oxide co-polymers NCO-sP(EO-stat-PO) when applied to gold surfaces as an auspicious coating material for gold sputtered blood contacting sensors. Quartz crystal microbalance (QCM) sensors were coated with ultrathin NCO-sP(EO-stat-PO) films and compared with uncoated gold sensors. Protein resistance was assessed by QCM measurements with fibrinogen solution and platelet poor plasma (PPP), followed by quantification of fibrinogen adsorption. Hemocompatibility was tested by incubation with human platelet rich plasma (PRP). Thrombin antithrombin-III complex (TAT), beta-thromboglobulin (beta-TG) and platelet factor 4 (PF4) were used as coagulation activation markers. Furthermore, scanning electron microscopy (SEM) was used to visualize platelet adhesion to the sensor surfaces. Compared to uncoated gold sensors, NCO-sP(EO-stat-PO) coated sensors revealed significant better resistance against protein adsorption, lower TAT generation and a lower amount of adherent platelets. Moreover, coating with ultrathin NCO-sP(EO-stat-PO) films creates a cell resistant hemocompatible surface on gold that increases the chance of prolonged sensor functionality and can easily be modified with specific receptor molecules.show moreshow less

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Author: Stefan Sinn, Mirjam Eichler, Lothar Müller, Daniel Bünger, Jüergen Groll, Gerhard Ziemer, Frank Rupp, Hinnak Northoff, Jürgen Geis-Gerstorfer, Frank K. Gehring, Hans P. Wendel
URN:urn:nbn:de:bvb:20-opus-141110
Document Type:Journal article
Faculties:Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):Sensors
Year of Completion:2011
Volume:11
Issue:5
Pagenumber:5253-5269
Source:Sensors 2011, 11, 5253-5269; doi:10.3390/s110505253
DOI:https://doi.org/10.3390/s110505253
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:316L stainless-steel; QCM; biosensor; biosensors; blood-plasma; cell coagulation; cell self-assembled monolayers; hemocompatibility; platelet-adhesion; poly(ethylene glycol); protein adsorption; self-assembled monolayers; surface coating; surfaces
Release Date:2018/12/12
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung