The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy

Please always quote using this URN: urn:nbn:de:bvb:20-opus-232292
  • Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of theExpansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of the Gram-positive pathogen Staphylococcus aureus. We further demonstrate the suitability of the technique for imaging of planktonic bacteria as well as endocytosed, intracellular bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.show moreshow less

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Metadaten
Author: Tobias C. Kunz, Marcel Rühling, Adriana Moldovan, Kerstin Paprotka, Vera Kozjak-Pavlovic, Thomas Rudel, Martin Fraunholz
URN:urn:nbn:de:bvb:20-opus-232292
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Frontiers in Cellular and Infection Microbiology
ISSN:2235-2988
Year of Completion:2021
Volume:11
Article Number:644750
Source:Frontiers in Cellular and Infection Microbiology 2021, 11:644750. DOI: 10.3389/fcimb.2021.644750
DOI:https://doi.org/10.3389/fcimb.2021.644750
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:autophagosomes; endosomes; expansion microscopy; high-resolution imaging; host-pathogen interaction
Release Date:2022/02/03
Open-Access-Publikationsfonds / Förderzeitraum 2021
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International