From Thermogelling Hydrogels toward Functional Bioinks: Controlled Modification and Cytocompatible Crosslinking

Please always quote using this URN: urn:nbn:de:bvb:20-opus-257542
  • Hydrogels are key components in bioink formulations to ensure printability and stability in biofabrication. In this study, a well-known Diels-Alder two-step post-polymerization modification approach is introduced into thermogelling diblock copolymers, comprising poly(2-methyl-2-oxazoline) and thermoresponsive poly(2-n-propyl-2-oxazine). The diblock copolymers are partially hydrolyzed and subsequently modified by acid/amine coupling with furan and maleimide moieties. While the thermogelling and shear-thinning properties allow excellentHydrogels are key components in bioink formulations to ensure printability and stability in biofabrication. In this study, a well-known Diels-Alder two-step post-polymerization modification approach is introduced into thermogelling diblock copolymers, comprising poly(2-methyl-2-oxazoline) and thermoresponsive poly(2-n-propyl-2-oxazine). The diblock copolymers are partially hydrolyzed and subsequently modified by acid/amine coupling with furan and maleimide moieties. While the thermogelling and shear-thinning properties allow excellent printability, trigger-less cell-friendly Diels-Alder click-chemistry yields long-term shape-fidelity. The introduced platform enables easy incorporation of cell-binding moieties (RGD-peptide) for cellular interaction. The hydrogel is functionalized with RGD-peptides using thiol-maleimide chemistry and cell proliferation as well as morphology of fibroblasts seeded on top of the hydrogels confirm the cell adhesion facilitated by the peptides. Finally, bioink formulations are tested for biocompatibility by incorporating fibroblasts homogenously inside the polymer solution pre-printing. After the printing and crosslinking process good cytocompatibility is confirmed. The established bioink system combines a two-step approach by physical precursor gelation followed by an additional chemical stabilization, offering a broad versatility for further biomechanical adaptation or bioresponsive peptide modification.show moreshow less

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Author: Lukas Hahn, Matthias Beudert, Marcus Gutmann, Larissa Keßler, Philipp Stahlhut, Lena Fischer, Emine Karakaya, Thomas Lorson, Ingo Thievessen, Rainer Detsch, Tessa Lühmann, Robert LuxenhoferORCiD
URN:urn:nbn:de:bvb:20-opus-257542
Document Type:Journal article
Faculties:Fakultät für Chemie und Pharmazie / Institut für Funktionsmaterialien und Biofabrikation
Language:English
Parent Title (English):Macromolecular Bioscience
Year of Completion:2021
Volume:21
Issue:10
Article Number:e2100122
Source:Macromolecular Bioscience 2021, 21(10):e2100122. DOI: 10.1002/mabi.202100122
DOI:https://doi.org/10.1002/mabi.202100122
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Tag:biofabrication; bioprinting; chemical crosslinking; hydrogels
Release Date:2022/03/22
Licence (German):License LogoCC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International