Red blood cell-derived microparticles exert no cancer promoting effects on colorectal cancer cells in vitro

Please always quote using this URN: urn:nbn:de:bvb:20-opus-286018
  • The biomedical consequences of allogeneic blood transfusions and the possible pathomechanisms of transfusion-related morbidity and mortality are still not entirely understood. In retrospective studies, allogeneic transfusion was associated with increased rates of cancer recurrence, metastasis and death in patients with colorectal cancer. However, correlation does not imply causation. The purpose of this study was to elucidate this empirical observation further in order to address insecurity among patients and clinicians. We focused on the inThe biomedical consequences of allogeneic blood transfusions and the possible pathomechanisms of transfusion-related morbidity and mortality are still not entirely understood. In retrospective studies, allogeneic transfusion was associated with increased rates of cancer recurrence, metastasis and death in patients with colorectal cancer. However, correlation does not imply causation. The purpose of this study was to elucidate this empirical observation further in order to address insecurity among patients and clinicians. We focused on the in vitro effect of microparticles derived from red blood cell units (RMPs). We incubated different colon carcinoma cells with RMPs and analyzed their effects on growth, invasion, migration and tumor marker expression. Furthermore, effects on Wnt, Akt and ERK signaling were explored. Our results show RMPs do not seem to affect functional and phenotypic characteristics of different colon carcinoma cells and did not induce or inhibit Wnt, Akt or ERK signaling, albeit in cell culture models lacking tumor microenvironment. Allogeneic blood transfusions are associated with poor prognosis, but RMPs do not seem to convey tumor-enhancing effects. Most likely, the circumstances that necessitate the transfusion, such as preoperative anemia, tumor stage, perioperative blood loss and extension of surgery, take center stage.show moreshow less

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Author: Dania Fischer, Fabian Thies, Omar Awad, Camilla Brat, Patrick MeybohmORCiD, Patrick C. Baer, Markus M. Müller, Anja Urbschat, Thorsten J. Maier, Kai Zacharowski, Jessica Roos
URN:urn:nbn:de:bvb:20-opus-286018
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Anästhesiologie (ab 2004)
Language:English
Parent Title (English):International Journal of Molecular Sciences
ISSN:1422-0067
Year of Completion:2022
Volume:23
Issue:16
Article Number:9323
Source:International Journal of Molecular Sciences (2022) 23:16, 9323. https://doi.org/10.3390/ijms23169323
DOI:https://doi.org/10.3390/ijms23169323
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:colorectal carcinoma; microparticles; red blood cells; transfusion
Release Date:2023/09/04
Date of first Publication:2022/08/18
EU-Project number / Contract (GA) number:101015930
EU-Project number / Contract (GA) number:101045956
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International