In-vitro cytotoxicity study: cell viability and cell morphology of carbon nanofibrous scaffold/hydroxyapatite nanocomposites
Please always quote using this URN: urn:nbn:de:bvb:20-opus-234037
- Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA;Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA; hydrothermally) to be used as a platform for bone tissue engineering. The biological investigations were performed using in-vitro cell counting, WST cell viability and cell morphology after three and seven days. L929 mouse fibroblasts were found to be more viable on the hydrothermally-modified CNF scaffolds than on the unmodified CNF scaffolds. The biological characterizations of the synthesized CNF/HA nanofibrous composites indicated higher capability of bone regeneration.…
| Author: | Asmaa M. Abd El-Aziz, Azza El-Maghraby, Andrea Ewald, Sherif H. Kandil |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-234037 |
| Document Type: | Journal article |
| Faculties: | Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde |
| Language: | English |
| Parent Title (English): | Molecules |
| ISSN: | 1420-3049 |
| Year of Completion: | 2021 |
| Volume: | 26 |
| Issue: | 6 |
| Article Number: | 1552 |
| Source: | Molecules (2021) 26:6, 1552. https://doi.org/10.3390/molecules26061552 |
| DOI: | https://doi.org/10.3390/molecules26061552 |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Tag: | HA modifiedCNF membranes; WST test; cell counting; cell morphology; cell viability; cytotoxicity |
| Release Date: | 2022/08/12 |
| Date of first Publication: | 2021/03/11 |
| Licence (German): |  CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |