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5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac
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- Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the ‘neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in theTwo 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the ‘neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.…
Autor(en): | Michael Schmid, Claus Steinlein, Christian Lomb, Karl Sperling, Heidemarie Neitzel |
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URN: | urn:nbn:de:bvb:20-opus-196701 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Humangenetik |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Cytogenetic and Genome Research |
ISSN: | 1424-8581 |
ISSN: | 1424-859X |
Erscheinungsjahr: | 2016 |
Band / Jahrgang: | 147 |
Heft / Ausgabe: | 4 |
Seitenangabe: | 240-246 |
Originalveröffentlichung / Quelle: | Cytogenetic and Genome Research 2015;147(4):240–246. DOI: 10.1159/000444431 |
DOI: | https://doi.org/10.1159/000444431 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/26959372 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | 5-Methylcytosine; Indian muntjac; heterochromatin; immunofluorescence |
Datum der Freischaltung: | 20.08.2021 |
Datum der Erstveröffentlichung: | 10.03.2016 |
Anmerkungen: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Lizenz (Deutsch): | Deutsches Urheberrecht |