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Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b.
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-60545
- In culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could beIn culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-tenninal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.…
Autor(en): | Jürgen Kreft, Dorothee Funke, Albert Haas, Friedrich Lottspeich, Werner Goebel |
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URN: | urn:nbn:de:bvb:20-opus-60545 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Erscheinungsjahr: | 1989 |
Originalveröffentlichung / Quelle: | In: FEMS Microbiology Letters (1989) 57, 2, S. 197 - 202. |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Normierte Schlagworte (GND): | Biologie |
Freie Schlagwort(e): | Hemolysin; Listeria |
Datum der Freischaltung: | 02.09.2011 |
Lizenz (Deutsch): | Deutsches Urheberrecht |