Different evolutionary modifications as a guide to rewire two-component systems
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-123647
- Two-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of TCS in different organisms, this study gives detailed data, concrete alignments, and structure analysis on three general modification scenarios, whereTwo-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of TCS in different organisms, this study gives detailed data, concrete alignments, and structure analysis on three general modification scenarios, where TCS were rewired for new responses and functions: (i) exchanges in the sequence within single TCS domains, (ii) exchange of whole TCS domains; (iii) addition of new components modulating TCS function. As a result, the replacement of stimulus and promotor cassettes to rewire TCS is well defined exploiting the alignments given here. The diverged TCS examples are non-trivial and the design is challenging. Designed connector proteins may also be useful to modify TCS in selected cases.…
Autor(en): | Beate Krueger, Torben Friedrich, Frank Förster, Jörg Bernhardt, Roy Gross, Thomas Dandekar |
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URN: | urn:nbn:de:bvb:20-opus-123647 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Bioinformatics and Biology Insights |
Erscheinungsjahr: | 2012 |
Band / Jahrgang: | 6 |
Seitenangabe: | 97-128 |
Originalveröffentlichung / Quelle: | Bioinformatics and Biology Insights 2012:6 97–128. doi: 10.4137/BBI.S9356 |
DOI: | https://doi.org/10.4137/BBI.S9356 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/PMC3348925 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 0 Informatik, Informationswissenschaft, allgemeine Werke / 00 Informatik, Wissen, Systeme / 004 Datenverarbeitung; Informatik |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Freie Schlagwort(e): | Mycoplasma; connector; engineering; histidine kinase; promoter; response regulator; sensor; sequence alignment; synthetic biology |
Datum der Freischaltung: | 22.12.2015 |
Anmerkungen: | This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |