Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel GtACR1

Please always quote using this URN: urn:nbn:de:bvb:20-opus-260925
  • Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO\(_2\) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl\(^-\) and NO\(_3\)\(^-\) currents of -1 to -2 nA. The anion currents depolarizeGuard cells control the aperture of plant stomata, which are crucial for global fluxes of CO\(_2\) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl\(^-\) and NO\(_3\)\(^-\) currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K+ channels and the extrusion of K+. As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.show moreshow less

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Metadaten
Author: Shouguang Huang, Meiqi Ding, M. Rob G. Roelfsema, Ingo Dreyer, Sönke Scherzer, Khaled A. S Al-Rasheid, Shiqiang Gao, Georg Nagel, Rainer Hedrich, Kai R. Konrad
URN:urn:nbn:de:bvb:20-opus-260925
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Parent Title (English):Science Advances
Year of Completion:2021
Volume:7
Issue:28
Article Number:eabg4619
Source:Science Advances (2021) 7:28, eabg4619. DOI: 10.1126/sciadv.abg4619
DOI:https://doi.org/10.1126/sciadv.abg4619
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Tag:Arabidopsis thaliana; CO2; H+-atpase; K+ channels; R-type; SLAC1; abscisic-acid activation; intact plants; potassium channel; signal transduction
Release Date:2022/04/05
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2021
Licence (German):License LogoCC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International