Filtern
Volltext vorhanden
- ja (14)
Gehört zur Bibliographie
- ja (14)
Dokumenttyp
Sprache
- Englisch (14)
Schlagworte
- MSCs (2)
- Medizin (2)
- chondrogenesis (2)
- mesenchymal stem cell (2)
- tissue engineering (2)
- Aseptic loosening (1)
- Bone marrow (1)
- Bone tumor (1)
- Cartilage regeneration (1)
- Chondrosarcoma (1)
- GDF-5 (1)
- Hip joint (1)
- IL1RA (1)
- Joint capsule (1)
- Ligamentum capitis femoris (1)
- Osteoarthritis (1)
- PMMA (1)
- R57A (1)
- TKA (1)
- Tissue engineering (1)
- Total knee arthroplasty (1)
- antibiotic elution (1)
- arthritis (1)
- biceps tendon (1)
- bone cement (1)
- bone marrow (1)
- cartilage (1)
- cartilage regeneration (1)
- cell-based therapies (1)
- cellular origin (1)
- chondrocytes (1)
- chondrogenic differentiation (1)
- chondrogenic hypertrophy (1)
- custom-made implant (1)
- defects (1)
- foamy virus (1)
- iTotal (1)
- implant positioning (1)
- in vitro (1)
- interleukin 1 receptor antagonist (1)
- knee axis (1)
- knee joint (1)
- knee osteoarthritis (1)
- knee replacement (1)
- mayo stem (1)
- mesenchymal stromal cell (1)
- minimal invasive surgery (1)
- osteoarthritis (1)
- patient-specific (1)
- patient-specific knee arthroplasty (1)
- pellet culture (1)
- periprosthetic infection (1)
- polymethylmethacrylate (1)
- repair (1)
- scaffolds (1)
- short hip stem (1)
- shoulder (1)
- spacer (1)
- stem cells (1)
- stimulation (1)
- tendon-derived stem cell (1)
- therapy (1)
- total hip arthroplasty (1)
- total knee arthroplasty (1)
- total knee replacement (1)
- transplantation (1)
- tricompartmental knee osteoarthritis (1)
- virus vectors (1)
Institut
- Lehrstuhl für Orthopädie (14) (entfernen)
Sonstige beteiligte Institutionen
Purpose
Hypertrophic cartilage is an important characteristic of osteoarthritis and can often be found in patients suffering from osteoarthritis. Although the exact pathomechanism remains poorly understood, hypertrophic de-differentiation of chondrocytes also poses a major challenge in the cell-based repair of hyaline cartilage using mesenchymal stromal cells (MSCs). While different members of the transforming growth factor beta (TGF-β) family have been shown to promote chondrogenesis in MSCs, the transition into a hypertrophic phenotype remains a problem. To further examine this topic we compared the effects of the transcription growth and differentiation factor 5 (GDF-5) and the mutant R57A on in vitro chondrogenesis in MSCs.
Methods
Bone marrow-derived MSCs (BMSCs) were placed in pellet culture and in-cubated in chondrogenic differentiation medium containing R57A, GDF-5 and TGF-ß1 for 21 days. Chondrogenesis was examined histologically, immunohistochemically, through biochemical assays and by RT-qPCR regarding the expression of chondrogenic marker genes.
Results
Treatment of BMSCs with R57A led to a dose dependent induction of chondrogenesis in BMSCs. Biochemical assays also showed an elevated glycosaminoglycan (GAG) content and expression of chondrogenic marker genes in corresponding pellets. While treatment with R57A led to superior chondrogenic differentiation compared to treatment with the GDF-5 wild type and similar levels compared to incubation with TGF-ß1, levels of chondrogenic hypertrophy were lower after induction with R57A and the GDF-5 wild type.
Conclusions
R57A is a stronger inducer of chondrogenesis in BMSCs than the GDF-5 wild type while leading to lower levels of chondrogenic hypertrophy in comparison with TGF-ß1.