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Erscheinungsjahr
- 2019 (1)
Dokumenttyp
Sprache
- Englisch (1)
Schlagworte
- Callyspongia siphonella (1)
- LC-HRESIMS (1)
- antibacterial (1)
- antibiofilm (1)
- anticancer (1)
- antitrypanosomal (1)
- metabolomic profiling (1)
- oxindole alkaloids (1)
- tisindoline (1)
Institut
- Abteilung für Molekulare Innere Medizin (in der Medizinischen Klinik und Poliklinik II) (1) (entfernen)
In the present study, LC-HRESIMS-assisted dereplication along with bioactivity-guided isolation led to targeting two brominated oxindole alkaloids (compounds 1 and 2) which probably play a key role in the previously reported antibacterial, antibiofilm, and cytotoxicity of Callyspongia siphonella crude extracts. Both metabolites showed potent antibacterial activity against Gram-positive bacteria, Staphylococcus aureus (minimum inhibitory concentration (MIC) = 8 and 4 µg/mL) and Bacillus subtilis (MIC = 16 and 4 µg/mL), respectively. Furthermore, they displayed moderate biofilm inhibitory activity in Pseudomonas aeruginosa (49.32% and 41.76% inhibition, respectively), and moderate in vitro antitrypanosomal activity (13.47 and 10.27 µM, respectively). In addition, they revealed a strong cytotoxic effect toward different human cancer cell lines, supposedly through induction of necrosis. This study sheds light on the possible role of these metabolites (compounds 1 and 2) in keeping fouling organisms away from the sponge outer surface, and the possible applications of these defensive molecules in the development of new anti-infective agents.