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Erscheinungsjahr
- 2020 (1)
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- Englisch (1) (entfernen)
Schlagworte
- Fluoreszenzresonanz-Energietransfer (1)
- Isomorphe Nukleobasen-Analoga (1)
- RNA aptamers (1)
- RNA-Aptamere (1)
- Stokes-Verschiebung (1)
- Struktursonden (1)
- fluorescence resonance energy transfer (1)
- isomorphic nucleobase analog (1)
- large stokes shift (1)
- stokes shift (1)
- structure probes (1)
- structure probing (1)
Institut
- Institut für Organische Chemie (1) (entfernen)
EU-Projektnummer / Contract (GA) number
- 682586 (1)
RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Here we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to bound ligands DMHBI+ or DMHBO+. The photophysical properties of the new nucleobase-ligand-FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET-based readout of ligand binding. This strategy is generally suitable for binding site mapping and may also be applied for responsive aptamer devices.