TY  - JOUR
A1  - Scheitl, Carolin P. M.
A1  - Lange, Sandra
A1  - Höbartner, Claudia
T1  - New deoxyribozymes for the native ligation of RNA
T2  - Molecules
N2  - Deoxyribozymes (DNAzymes) are small, synthetic, single-stranded DNAs capable of catalysing chemical reactions, including RNA ligation. Herein, we report a novel class of RNA ligase deoxyribozymes that utilize 5’-adenylated RNA (5’-AppRNA) as the donor substrate, mimicking the activated intermediates of protein-catalyzed RNA ligation. Four new DNAzymes were identified by in vitro selection from an N40 random DNA library and were shown to catalyze the intermolecular linear RNA-RNA ligation via the formation of a native 3’-5’-phosphodiester linkage. The catalytic activity is distinct from previously described RNA-ligating deoxyribozymes. Kinetic analyses revealed the optimal incubation conditions for high ligation yields and demonstrated a broad RNA substrate scope. Together with the smooth synthetic accessibility of 5’-adenylated RNAs, the new DNA enzymes are promising tools for the protein-free synthesis of long RNAs, for example containing precious modified nucleotides or fluorescent labels for biochemical and biophysical investigations.
KW  - RNA ligation
KW  - DNA catalysis
KW  - in vitro selection
KW  - Deoxyribozyme
Y1  - 2020
UR  - https://opus.bibliothek.uni-wuerzburg.de/opus4-wuerzburg/frontdoor/index/index/docId/21040
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-210405
VL  - 25
IS  - 16
ER  -