@inproceedings{FrankeZentgrafScheer1978, author = {Franke, Werner W. and Zentgraf, Hanswalter and Scheer, Ulrich}, title = {Supranucleosomal and non-nucleosomal chromatin configurations}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39447}, year = {1978}, abstract = {A significant contribution to the understanding of chromatin organization was the d iscovery of the nucleosome as a globular repeating unit of the package of DNA (Hewish and Burgoyne, 1973; Woodcock, 1973; Kornberg, 1974; Olins and Olins, 1974; for review see Oudet et al., 1978 a) . In accord with the original definition and in ag reement with most workers in this field of research we identify a nucleosome as a spheric alor slightly oblate gr anular particle 10-13 nm in diameter, containing about 200 base pairs of DNA and two of each of the four his tones H2a, H2b, H3 and H4. It is this structure in which the bulk of the nuclear chroma tin is organized in most eukaryotic cells, with the exception of the dinofl age llates (Rae and Steele, 1977; dinofl agellate DNA, however, c an be packed into nucleosoma l structures in vitro by addition of the appropriate amounts of histones;the same reference). Although it seems clear from the work reported that condensed and transcriptiona lly inactive chroma tin is contained in nucleosomes as the principle for first order p acking of DNA there are two important questions onto which we are focusing in the present study: ( i ) What is the higher order of p a cking present in - and perhaps typical-of - the condensed sta te of chromatin, and (ii) what is the specific form of arrangement of transcriptionally a ctive chromatin?}, language = {en} } @article{FrankeScheerZentgraf1984, author = {Franke, Werner W. and Scheer, Ulrich and Zentgraf, Hanswalter}, title = {Organization of transcriptionally active and inactive chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40588}, year = {1984}, abstract = {No abstract available}, subject = {Deutschland}, language = {en} } @incollection{FrankeScheerZentgrafetal.1980, author = {Franke, Werner W. and Scheer, Ulrich and Zentgraf, Hanswalter and Trendelenburg, Michael F. and M{\"u}ller, U. and Krohne, G. and Spring, H.}, title = {Organization of transcribed and nontranscribed chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40656}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1980}, abstract = {No abstract available}, subject = {Tumor / Zellteilung}, language = {en} } @incollection{FrankeScheerSpringetal.1979, author = {Franke, Werner W. and Scheer, Ulrich and Spring, Herbert and Trendelenburg, Michael F. and Zentgraf, Hanswalter}, title = {Organization of nucleolar chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39410}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1979}, abstract = {No abstract available}, language = {en} } @inproceedings{ZentgrafScheerFranke1976, author = {Zentgraf, Hanswalter and Scheer, Ulrich and Franke, Werner W.}, title = {On the existence of arrested transcriptional machinery in late stages of avian erythropoiesis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33696}, year = {1976}, abstract = {No abstract available}, language = {de} } @article{ZentgrafTrendelenburgSpringetal.1979, author = {Zentgraf, Hanswalter and Trendelenburg, Michael F. and Spring, Herbert and Scheer, Ulrich and Franke, Werner W. and M{\"u}ller, Ulrike and Drury, Kenneth C. and Rungger, Duri}, title = {Mitochondrial DNA arranged into chromatin-like structures after injection into amphibian oocyte nuclei}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33174}, year = {1979}, abstract = {Purified mitochondrial DNA (mitDNA) from ovaries ofXenopus lae vis was injected into the nuclei (germinal vesicles) of large viteUogenic oocytes of the same organism and examined by electron microscopy ofthe spread nuclear contents. Normally located nuclei of untreated oocytes as weil as peripherally translocated nuclei of centrifuged oocytes were used. In addition, oocyte nuclei isolated and incubated under liquid paraffin oil were injected with DNA. The integrity oftranscriptional structures of endogenous chromosomal (Iampbrush chromosomes) and extrachromosomal (nucleoli) genes of the injected nuclei was demonstrated. Microinjected mitDN A was identified as circles of chromatin exhibiting polynucleosome-like organization and a me an contour length of 2.6 J.Lm, corresponding to a compaction ratio of the mitDN A of about 2 : I. This DNA packing ratio is similar to that observed after preparation of various kinds of native chromatin in low salt buffers. The chromatin circles formed from injected mitDNA only very rarely exhibited lateral fibrils suggestive of transcriptional activity. These results suggest that purified mitDNA can be transformed to normally structured chromatin when exposed to oocyte nuclear contents but is rarely , if at all , transcribed in this form and in this environment.}, language = {en} } @article{FrankeKartenbeckZentgrafetal.1971, author = {Franke, Werner W. and Kartenbeck, J{\"u}rgen and Zentgraf, Hanswalter and Scheer, Ulrich and Falk, Heinz}, title = {Membrane-to-membrane cross-bridges. A means to orientation and interaction of membrane faces}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32122}, year = {1971}, abstract = {No abstract available}, language = {en} } @article{FrankeZentgrafScheer1973, author = {Franke, Werner W. and Zentgraf, Hanswalter and Scheer, Ulrich}, title = {Membrane linkages at the nuclear envelope}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40596}, year = {1973}, abstract = {Electron-opaque material is shown in the perinuclear cisternae of various cell types to connect the inner and outer nuclear membrane faces. Similar bridges were observed between the outer nuclear membrane and the outer mitochondrial membrane. The intracisternal bridges of the nuclear envelope appear to be important for the structural stability of the perinuclear cisterna. Stable structural linkage of mitochondria to the outer nuclear membrane might be relevant to the understanding of the characteristic juxtanuclear accumulation of mitochondria and also provide arguments for the discussions of certain biochemical activities found in nuclear and nuclear membrane fractions.}, subject = {Cytologie}, language = {en} } @article{TrendelenburgScheerZentgrafetal.1976, author = {Trendelenburg, Michael F. and Scheer, Ulrich and Zentgraf, Hanswalter and Franke, Werner W.}, title = {Heterogeneity of spacer lengths in circles of amplified ribosomal DNA of two insect species, Dytiscus marginalis and Acheta domesticus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33055}, year = {1976}, abstract = {No abstract available}, language = {en} } @article{ZentgrafScheerFranke1975, author = {Zentgraf, Hanswalter and Scheer, Ulrich and Franke, Werner W.}, title = {Characterization and localization of the RNA synthesized in mature avian erythrocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32410}, year = {1975}, abstract = {No abstract available}, language = {en} }