@article{SchmidtEbnerRosenetal.2020,
  author    = {Schmidt, Stefanie and Ebner, Friederike and Rosen, Kerstin and Kniemeyer, Olaf and Brakhage, Axel A. and L{\"o}ffler, J{\"u}rgen and Seif, Michelle and Springer, Jan and Schlosser, Josephine and Scharek-Tedin, Lydia and Scheffold, Alexander and Bacher, Petra and K{\"u}hl, Anja A. and R{\"o}sler, Uwe and Hartmann, Susanne},
  title     = {The domestic pig as human-relevant large animal model to study adaptive antifungal immune responses against airborne Aspergillus fumigatus},
  series = {European Journal of Immunology},
  volume    = {50},
  journal   = {European Journal of Immunology},
  number    = {11},
  doi       = {10.1002/eji.201948524},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-216085},
  pages     = {1712 -- 1728},
  year      = {2020},
  abstract  = {Pulmonary mucosal immune response is critical for preventing opportunistic Aspergillus fumigatus infections. Although fungus-specific CD4\(^{+}\) T cells in blood are described to reflect the actual host-pathogen interaction status, little is known about Aspergillus-specific pulmonary T-cell responses. Here, we exploit the domestic pig as human-relevant large animal model and introduce antigen-specific T-cell enrichment in pigs to address Aspergillus-specific T cells in the lung compared to peripheral blood. In healthy, environmentally Aspergillus-exposed pigs, the fungus-specific T cells are detectable in blood in similar frequencies as observed in healthy humans and exhibit a Th1 phenotype. Exposing pigs to 10\(^{6}\) cfu/m\(^{3}\) conidia induces a long-lasting accumulation of Aspergillus-specific Th1 cells locally in the lung and also systemically. Temporary immunosuppression during Aspergillus-exposure showed a drastic reduction in the lung-infiltrating antifungal T-cell responses more than 2 weeks after abrogation of the suppressive treatment. This was reflected in blood, but to a much lesser extent. In conclusion, by using the human-relevant large animal model the pig, this study highlights that the blood clearly reflects the mucosal fungal-specific T-cell reactivity in environmentally exposed as well as experimentally exposed healthy pigs. But, immunosuppression significantly impacts the mucosal site in contrast to the initial systemic immune response.},
  language  = {en}
}
@article{RethwilmDaraiRoesenetal.1987,
  author    = {Rethwilm, Axel and Darai, G. and R{\"o}sen, A. and Maurer, Bernd and Fl{\"u}gel, Rolf M.},
  title     = {Molecular cloning of the genome of human spumaretrovirus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61518},
  year      = {1987},
  abstract  = {DNA ofhuman spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage A and bacterial plasmid vectors. The recombinant plasm.ids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained from viral DNA. The recombinant clones isolated contained viral DNA inserts which rangein size from 2.2 kb to 15.4 kb. The recombinant clones allowed to construct a physical map of the complete HSRV provirus of 12.2 kb.},
  subject      = {Virologie},
  language  = {en}
}