@article{SchaefferKuehnSchmittetal.2013, author = {Schaeffer, Evelin L. and K{\"u}hn, Franziska and Schmitt, Angelika and Gattaz, Wagner F. and Gruber, Oliver and Schneider-Axmann, Thomas and Falkai, Peter and Schmitt, Andrea}, title = {Increased cell proliferation in the rat anterior cingulate cortex following neonatal hypoxia: relevance to schizophrenia}, series = {Journal of Neural Transmission}, volume = {120}, journal = {Journal of Neural Transmission}, number = {1}, doi = {10.1007/s00702-012-0859-y}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-125890}, pages = {187-195}, year = {2013}, abstract = {As a consequence of obstetric complications, neonatal hypoxia has been discussed as an environmental factor in the pathophysiology of schizophrenia. However, the biological consequences of hypoxia are unclear. The neurodevelopmental hypothesis of schizophrenia suggests that the onset of abnormal brain development and neuropathology occurs perinatally, whereas symptoms of the disease appear in early adulthood. In our animal model of chronic neonatal hypoxia, we have detected behavioral alterations resembling those known from schizophrenia. Disturbances in cell proliferation possibly contribute to the pathophysiology of this disease. In the present study, we used postnatal rats to investigate cell proliferation in several brain areas following neonatal hypoxia. Rats were repeatedly exposed to hypoxia (89 \% N2, 11 \% O2) from postnatal day (PD) 4-8. We then evaluated cell proliferation on PD 13 and 39, respectively. These investigations were performed in the anterior cingulate cortex (ACC), caudate-putamen (CPU), dentate gyrus, and subventricular zone. Rats exposed to hypoxia exhibited increased cell proliferation in the ACC at PD 13, normalizing at PD 39. In other brain regions, no alterations have been detected. Additionally, hypoxia-treated rats showed decreased CPU volume at PD 13. The results of the present study on the one hand support the assumption of chronic hypoxia influencing transient cell proliferation in the ACC, and on the other hand reveal normalization during ageing.}, language = {en} } @article{KuehnemundtLeifeldSchergetal.2021, author = {K{\"u}hnemundt, Johanna and Leifeld, Heidi and Scherg, Florian and Schmitt, Matthias and Nelke, Lena C. and Schmitt, Tina and Bauer, Florentin and G{\"o}ttlich, Claudia and Fuchs, Maximilian and Kunz, Meik and Peindl, Matthias and Br{\"a}hler, Caroline and Kronenthaler, Corinna and Wischhusen, J{\"o}rg and Prelog, Martina and Walles, Heike and Dandekar, Thomas and Dandekar, Gudrun and Nietzer, Sarah L.}, title = {Modular micro-physiological human tumor/tissue models based on decellularized tissue for improved preclinical testing}, series = {ALTEX}, volume = {38}, journal = {ALTEX}, doi = {10.14573/altex.2008141}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-231465}, pages = {289-306}, year = {2021}, abstract = {High attrition-rates entailed by drug testing in 2D cell culture and animal models stress the need for improved modeling of human tumor tissues. In previous studies our 3D models on a decellularized tissue matrix have shown better predictivity and higher chemoresistance. A single porcine intestine yields material for 150 3D models of breast, lung, colorectal cancer (CRC) or leukemia. The uniquely preserved structure of the basement membrane enables physiological anchorage of endothelial cells and epithelial-derived carcinoma cells. The matrix provides different niches for cell growth: on top as monolayer, in crypts as aggregates and within deeper layers. Dynamic culture in bioreactors enhances cell growth. Comparing gene expression between 2D and 3D cultures, we observed changes related to proliferation, apoptosis and stemness. For drug target predictions, we utilize tumor-specific sequencing data in our in silico model finding an additive effect of metformin and gefitinib treatment for lung cancer in silico, validated in vitro. To analyze mode-of-action, immune therapies such as trispecific T-cell engagers in leukemia, as well as toxicity on non-cancer cells, the model can be modularly enriched with human endothelial cells (hECs), immune cells and fibroblasts. Upon addition of hECs, transmigration of immune cells through the endothelial barrier can be investigated. In an allogenic CRC model we observe a lower basic apoptosis rate after applying PBMCs in 3D compared to 2D, which offers new options to mirror antigen-specific immunotherapies in vitro. In conclusion, we present modular human 3D tumor models with tissue-like features for preclinical testing to reduce animal experiments.}, language = {en} } @article{FrankSchmittHovestadtetal.2017, author = {Frank, Erik Thomas and Schmitt, Thomas and Hovestadt, Thomas and Mitesser, Oliver and Stiegler, Jonas and Linsenmair, Karl Eduard}, title = {Saving the injured: Rescue behavior in the termite-hunting ant Megaponera analis}, series = {Science Advances}, volume = {3}, journal = {Science Advances}, number = {4}, doi = {10.1126/sciadv.1602187}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-157933}, pages = {e1602187}, year = {2017}, abstract = {Predators of highly defensive prey likely develop cost-reducing adaptations. The ant Megaponera analis is a specialized termite predator, solely raiding termites of the subfamily Macrotermitinae (in this study, mostly colonies of Pseudocanthotermes sp.) at their foraging sites. The evolutionary arms race between termites and ants led to various defensive mechanisms in termites (for example, a caste specialized in fighting predators). Because M. analis incurs high injury/mortality risks when preying on termites, some risk-mitigating adaptations seem likely to have evolved. We show that a unique rescue behavior in M. analis, consisting of injured nestmates being carried back to the nest, reduces combat mortality. After a fight, injured ants are carried back by their nestmates; these ants have usually lost an extremity or have termites clinging to them and are able to recover within the nest. Injured ants that are forced experimentally to return without help, die in 32\% of the cases. Behavioral experiments show that two compounds, dimethyl disulfide and dimethyl trisulfide, present in the mandibular gland reservoirs, trigger the rescue behavior. A model accounting for this rescue behavior identifies the drivers favoring its evolution and estimates that rescuing enables maintenance of a 28.7\% larger colony size. Our results are the first to explore experimentally the adaptive value of this form of rescue behavior focused on injured nestmates in social insects and help us to identify evolutionary drivers responsible for this type of behavior to evolve in animals.}, language = {en} } @article{PfennigLeopoldBechdolfetal.2014, author = {Pfennig, Andrea and Leopold, Karolina and Bechdolf, Andreas and Correll, Christoph U. and Holtmann, Martin and Lambert, Martin and Marx, Carolin and Meyer, Thomas D. and Pfeiffer, Steffi and Reif, Andreas and Rottmann-Wolf, Maren and Schmitt, Natalie M. and Stamm, Thomas and Juckel, Georg and Bauer, Michael}, title = {Early specific cognitive-behavioural psychotherapy in subjects at high risk for bipolar disorders: study protocol for a randomised controlled trial}, series = {TRIALS}, volume = {15}, journal = {TRIALS}, number = {161}, issn = {1468-6694}, doi = {10.1186/1745-6215-15-161}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-116279}, year = {2014}, abstract = {Background: Bipolar disorders (BD) are among the most severe mental disorders with first clinical signs and symptoms frequently appearing in adolescence and early adulthood. The long latency in clinical diagnosis (and subsequent adequate treatment) adversely affects the course of disease, effectiveness of interventions and health-related quality of life, and increases the economic burden of BD. Despite uncertainties about risk constellations and symptomatology in the early stages of potentially developing BD, many adolescents and young adults seek help, and most of them suffer substantially from symptoms already leading to impairments in psychosocial functioning in school, training, at work and in their social relationships. We aimed to identify subjects at risk of developing BD and investigate the efficacy and safety of early specific cognitive-behavioural psychotherapy (CBT) in this subpopulation. Methods/Design: EarlyCBT is a randomised controlled multi-centre clinical trial to evaluate the efficacy and safety of early specific CBT, including stress management and problem solving strategies, with elements of mindfulness-based therapy (MBT) versus unstructured group meetings for 14 weeks each and follow-up until week 78. Participants are recruited at seven university hospitals throughout Germany, which provide in-and outpatient care (including early recognition centres) for psychiatric patients. Subjects at high risk must be 15 to 30 years old and meet the combination of specified affective symptomatology, reduction of psychosocial functioning, and family history for (schizo) affective disorders. Primary efficacy endpoints are differences in psychosocial functioning and defined affective symptomatology at 14 weeks between groups. Secondary endpoints include the above mentioned endpoints at 7, 24, 52 and 78 weeks and the change within groups compared to baseline; perception of, reaction to and coping with stress; and conversion to full BD. Discussion: To our knowledge, this is the first study to evaluate early specific CBT in subjects at high risk for BD. Structured diagnostic interviews are used to map the risk status and development of disease. With our study, the level of evidence for the treatment of those young patients will be significantly raised.}, language = {en} } @article{CastilloWurdackPaulietal.2022, author = {Castillo, Ruth and Wurdack, Mareike and Pauli, Thomas and Keller, Alexander and Feldhaar, Heike and Polidori, Carlo and Niehuis, Oliver and Schmitt, Thomas}, title = {Evidence for a chemical arms race between cuckoo wasps of the genus Hedychrum and their distantly related host apoid wasps}, series = {BMC Ecology and Evolution}, volume = {22}, journal = {BMC Ecology and Evolution}, number = {1}, doi = {10.1186/s12862-022-02093-8}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-301289}, year = {2022}, abstract = {Background Brood parasites can exert strong selection pressure on their hosts. Many brood parasites escape their detection by mimicking sensory cues of their hosts. However, there is little evidence whether or not the hosts are able to escape the parasites' mimicry by changing these cues. We addressed this question by analyzing cuticular hydrocarbon (CHC) profiles of Cerceris and Philanthus wasps and their brood parasites, cuckoo wasps mimicking the CHC profiles of their hosts. Some of these hosts use hydrocarbons to preserve their prey against fungal infestation and thus, they cannot significantly change their CHC composition in response to chemical mimicry by Hedychrum brood parasites. Results We found that the CHC overlap between brood parasites and their hosts was lower in case of host wasps not preserving their prey than in case of prey-preserving host wasps, whose CHC evolution is constrained. Furthermore, the CHC profiles in non-preserving host wasps is more strongly diversified in females than in males, thus in the sex that is chemically mimicked by brood parasites. Conclusion Our results provide evidence for a chemical arms race between those hosts that are liberated from stabilizing selection on their chemical template and their parasites.}, language = {en} } @article{SchilbachAlkhaledWelkeretal.2015, author = {Schilbach, Karin and Alkhaled, Mohammed and Welker, Christian and Eckert, Franziska and Blank, Gregor and Ziegler, Hendrik and Sterk, Marco and M{\"u}ller, Friederike and Sonntag, Katja and Wieder, Thomas and Braum{\"u}ller, Heidi and Schmitt, Julia and Eyrich, Matthias and Schleicher, Sabine and Seitz, Christian and Erbacher, Annika and Pichler, Bernd J. and M{\"u}ller, Hartmut and Tighe, Robert and Lim, Annick and Gillies, Stephen D. and Strittmatter, Wolfgang and R{\"o}cken, Martin and Handgretinger, Rupert}, title = {Cancer-targeted IL-12 controls human rhabdomyosarcoma by senescence induction and myogenic differentiation}, series = {OncoImmunology}, volume = {4}, journal = {OncoImmunology}, number = {7}, doi = {10.1080/2162402X.2015.1014760}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-154579}, pages = {e1014760}, year = {2015}, abstract = {Stimulating the immune system to attack cancer is a promising approach, even for the control of advanced cancers. Several cytokines that promote interferon-γ-dominated immune responses show antitumor activity, with interleukin 12 (IL-12) being of major importance. Here, we used an antibody-IL-12 fusion protein (NHS-IL12) that binds histones of necrotic cells to treat human sarcoma in humanized mice. Following sarcoma engraftment, NHS-IL12 therapy was combined with either engineered IL-7 (FcIL-7) or IL-2 (IL-2MAB602) for continuous cytokine bioavailability. NHS-IL12 strongly induced innate and adaptive antitumor immunity when combined with IL-7 or IL-2. NHS-IL12 therapy significantly improved survival of sarcoma-bearing mice and caused long-term remissions when combined with IL-2. NHS-IL12 induced pronounced cancer cell senescence, as documented by strong expression of senescence-associated p16\(^{INK4a}\) and nuclear translocation of p-HP1γ, and permanent arrest of cancer cell proliferation. In addition, this cancer immunotherapy initiated the induction of myogenic differentiation, further promoting the hypothesis that efficient antitumor immunity includes mechanisms different from cytotoxicity for efficient cancer control in vivo.}, language = {en} } @article{SchmittFunkBlumetal.2016, author = {Schmitt, Dominique and Funk, Natalia and Blum, Robert and Asan, Esther and Andersen, Lill and R{\"u}licke, Thomas and Sendtner, Michael and Buchner, Erich}, title = {Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons}, series = {Histochemistry and Cell Biology}, volume = {146}, journal = {Histochemistry and Cell Biology}, number = {4}, doi = {10.1007/s00418-016-1457-0}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-187258}, pages = {489-512}, year = {2016}, abstract = {Synapse-associated protein 1 (Syap1/BSTA) is the mammalian homologue of Sap47 (synapse-associated protein of 47 kDa) in Drosophila. Sap47 null mutant larvae show reduced short-term synaptic plasticity and a defect in associative behavioral plasticity. In cultured adipocytes, Syap1 functions as part of a complex that phosphorylates protein kinase B alpha/Akt1 (Akt1) at Ser\(^{473}\) and promotes differentiation. The role of Syap1 in the vertebrate nervous system is unknown. Here, we generated a Syap1 knock-out mouse and show that lack of Syap1 is compatible with viability and fertility. Adult knock-out mice show no overt defects in brain morphology. In wild-type brain, Syap1 is found widely distributed in synaptic neuropil, notably in regions rich in glutamatergic synapses, but also in perinuclear structures associated with the Golgi apparatus of specific groups of neuronal cell bodies. In cultured motoneurons, Syap1 is located in axons and growth cones and is enriched in a perinuclear region partially overlapping with Golgi markers. We studied in detail the influence of Syap1 knockdown and knockout on structure and development of these cells. Importantly, Syap1 knockout does not affect motoneuron survival or axon growth. Unexpectedly, neither knockdown nor knockout of Syap1 in cultured motoneurons is associated with reduced Ser\(^{473}\) or Thr\(^{308}\) phosphorylation of Akt. Our findings demonstrate a widespread expression of Syap1 in the mouse central nervous system with regionally specific distribution patterns as illustrated in particular for olfactory bulb, hippocampus, and cerebellum.}, language = {en} } @article{UllmannSchmittJagdhuber2016, author = {Ullmann, Tobias and Schmitt, Andreas and Jagdhuber, Thomas}, title = {Two Component Decomposition of Dual Polarimetric HH/VV SAR Data: Case Study for the Tundra Environment of the Mackenzie Delta Region, Canada}, series = {Remote Sensing}, volume = {8}, journal = {Remote Sensing}, number = {12}, doi = {10.3390/rs8121027}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-147879}, pages = {1027}, year = {2016}, abstract = {This study investigates a two component decomposition technique for HH/VV-polarized PolSAR (Polarimetric Synthetic Aperture Radar) data. The approach is a straight forward adaption of the Yamaguchi decomposition and decomposes the data into two scattering contributions: surface and double bounce under the assumption of a negligible vegetation scattering component in Tundra environments. The dependencies between the features of this two and the classical three component Yamaguchi decomposition were investigated for Radarsat-2 (quad) and TerraSAR-X (HH/VV) data for the Mackenzie Delta Region, Canada. In situ data on land cover were used to derive the scattering characteristics and to analyze the correlation among the PolSAR features. The double bounce and surface scattering features of the two and three component scattering model (derived from pseudo-HH/VV- and quad-polarized data) showed similar scattering characteristics and positively correlated-R2 values of 0.60 (double bounce) and 0.88 (surface scattering) were observed. The presence of volume scattering led to differences between the features and these were minimized for land cover classes of low vegetation height that showed little volume scattering contribution. In terms of separability, the quad-polarized Radarsat-2 data offered the best separation of the examined tundra land cover types and will be best suited for the classification. This is anticipated as it represents the largest feature space of all tested ones. However; the classes "wetland" and "bare ground" showed clear positions in the feature spaces of the C- and X-Band HH/VV-polarized data and an accurate classification of these land cover types is promising. Among the possible dual-polarization modes of Radarsat-2 the HH/VV was found to be the favorable mode for the characterization of the aforementioned tundra land cover classes due to the coherent acquisition and the preserved co-pol. phase. Contrary, HH/HV-polarized and VV/VH-polarized data were found to be best suited for the characterization of mixed and shrub dominated tundra.}, language = {en} } @article{PollingerSchmittSanderetal.2017, author = {Pollinger, Florian and Schmitt, Stefan and Sander, Dirk and Tian, Zhen and Kirschner, J{\"u}rgen and Vrdoljak, Pavo and Stadler, Christoph and Maier, Florian and Marchetto, Helder and Schmidt, Thomas and Sch{\"o}ll, Achim and Umbach, Eberhard}, title = {Nanoscale patterning, macroscopic reconstruction, and enhanced surface stress by organic adsorption on vicinal surfaces}, series = {New Journal of Physics}, volume = {19}, journal = {New Journal of Physics}, doi = {10.1088/1367-2630/aa55b8}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-171947}, year = {2017}, abstract = {Self-organization is a promising method within the framework of bottom-up architectures to generate nanostructures in an efficient way. The present work demonstrates that self- organization on the length scale of a few to several tens of nanometers can be achieved by a proper combination of a large (organic) molecule and a vicinal metal surface if the local bonding of the molecule on steps is significantly stronger than that on low-index surfaces. In this case thermal annealing may lead to large mass transport of the subjacent substrate atoms such that nanometer-wide and micrometer-long molecular stripes or other patterns are being formed on high-index planes. The formation of these patterns can be controlled by the initial surface orientation and adsorbate coverage. The patterns arrange self-organized in regular arrays by repulsive mechanical interactions over long distances accompanied by a significant enhancement of surface stress. We demonstrate this effect using the planar organic molecule PTCDA as adsorbate and Ag(10 8 7) and Ag(775)surfaces as substrate. The patterns are directly observed by STM, the formation of vicinal surfaces is monitored by highresolution electron diffraction, the microscopic surface morphology changes are followed by spectromicroscopy, and the macroscopic changes of surface stress are measured by a cantilever bending method. The in situ combination of these complementary techniques provides compelling evidence for elastic interaction and a significant stress contribution to long-range order and nanopattern formation.}, language = {en} } @article{FeldheimKesslerSchmittetal.2018, author = {Feldheim, Jonas and Kessler, Almuth F and Schmitt, Dominik and Wilczek, Lara and Linsenmann, Thomas and Dahlmann, Mathias and Monoranu, Camelia M and Ernestus, Ralf-Ingo and Hagemann, Carsten and L{\"o}hr, Mario}, title = {Expression of activating transcription factor 5 (ATF5) is increased in astrocytomas of different WHO grades and correlates with survival of glioblastoma patients}, series = {OncoTargets and Therapy}, volume = {11}, journal = {OncoTargets and Therapy}, doi = {10.2147/OTT.S176549}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-177541}, pages = {8673-8684}, year = {2018}, abstract = {Background: ATF5 suppresses differentiation of neuroprogenitor cells and is overexpressed in glioblastoma (GBM). A reduction of its expression leads to apoptotic GBM cell death. Data on ATF5 expression in astrocytoma WHO grade II (low-grade astrocytoma [LGA]) are scarce and lacking on recurrent GBM. Patients and methods: ATF5 mRNA was extracted from frozen samples of patients' GBM (n=79), LGA (n=40), and normal brain (NB, n=10), quantified by duplex qPCR and correlated with retrospectively collected clinical data. ATF5 protein expression was evaluated by measuring staining intensity on immunohistochemistry. Results: ATF5 mRNA was overexpressed in LGA (sevenfold, P<0.001) and GBM (tenfold, P<0.001) compared to NB, which was confirmed on protein level. Although ATF5 mRNA expression in GBM showed a considerable fluctuation range, groups of varying biological behavior, that is, local/multifocal growth or primary tumor/relapse and the tumor localization at diagnosis, were not significantly different. ATF5 mRNA correlated with the patients' age (r=0.339, P=0.028) and inversely with Ki67-staining (r=-0.421, P=0.007). GBM patients were allocated to a low and a high ATF5 expression group by the median ATF5 overexpression compared to NB. Kaplan-Meier analysis and Cox regression indicated that ATF5 mRNA expression significantly correlated with short-term survival (t<12 months, median survival 18 vs 13 months, P=0.022, HR 2.827) and progression-free survival (PFS) (12 vs 6 months, P=0.024). This advantage vanished after 24 months (P=0.084). Conclusion: ATF5 mRNA expression could be identified as an additional, though not independent factor correlating with overall survival and PFS. Since its inhibition might lead to the selective death of glioma cells, it might serve as a potential ubiquitous therapeutic target in astrocytic tumors.}, language = {en} } @article{LeonhardtSchmittBluethgen2011, author = {Leonhardt, Sara D. and Schmitt, Thomas and Bl{\"u}thgen, Nico}, title = {Tree Resin Composition, Collection Behavior and Selective Filters Shape Chemical Profiles of Tropical Bees (Apidae: Meliponini)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-69035}, year = {2011}, abstract = {The diversity of species is striking, but can be far exceeded by the chemical diversity of compounds collected, produced or used by them. Here, we relate the specificity of plant-consumer interactions to chemical diversity applying a comparative network analysis to both levels. Chemical diversity was explored for interactions between tropical stingless bees and plant resins, which bees collect for nest construction and to deter predators and microbes. Resins also function as an environmental source for terpenes that serve as appeasement allomones and protection against predators when accumulated on the bees' body surfaces. To unravel the origin of the bees' complex chemical profiles, we investigated resin collection and the processing of resin-derived terpenes. We therefore analyzed chemical networks of tree resins, foraging networks of resin collecting bees, and their acquired chemical networks. We revealed that 113 terpenes in nests of six bee species and 83 on their body surfaces comprised a subset of the 1,117 compounds found in resins from seven tree species. Sesquiterpenes were the most variable class of terpenes. Albeit widely present in tree resins, they were only found on the body surface of some species, but entirely lacking in others. Moreover, whereas the nest profile of Tetragonula melanocephala contained sesquiterpenes, its surface profile did not. Stingless bees showed a generalized collecting behavior among resin sources, and only a hitherto undescribed species-specific ''filtering'' of resin-derived terpenes can explain the variation in chemical profiles of nests and body surfaces fromdifferent species. The tight relationship between bees and tree resins of a large variety of species elucidates why the bees' surfaces contain a much higher chemodiversity than other hymenopterans.}, subject = {Stachellose Biene}, language = {en} } @article{KerkauSchmittLandgrafSchimpletal.1989, author = {Kerkau, Thomas and Schmitt-Landgraf, Renate and Schimpl, Anneliese and Wecker, Eberhard}, title = {Downregulation of HLA Class I Antigensin HIV-1-Infected Cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47172}, year = {1989}, abstract = {By means of indirect immunofluorescence analysis we investigated the effect of HIV -1 infection on HLA class I surface antigens. We report here that in CD4\(^+\) HeLa cells, in H9 cells, and in peripheral T Iymphocytes HLA class I antigens are down regulated following infection with HIV -1. The downregulation is effected at a posttranscriptional level since the amounts of HLA class I specific mRNA are similar in infected and uninfected cells. This phenomenon is not only correlated with the state of infection, that is, the presence of P24 of HIV-l in the cells, but also depends on the time of infection. Upon HLA class I downregulation by HIV infection, the specific lysis of peripheral blood cells by allogeneic CTL is reduced.}, language = {en} } @article{DrescherBluethgenSchmittetal.2010, author = {Drescher, Jochen and Bluethgen, Nico and Schmitt, Thomas and Buehler, Jana and Feldhaar, Heike}, title = {Societies Drifting Apart? Behavioural, Genetic and Chemical Differentiation between Supercolonies in the Yellow Crazy Ant Anoplolepis gracilipes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-68573}, year = {2010}, abstract = {Background: In populations of most social insects, gene flow is maintained through mating between reproductive individuals from different colonies in periodic nuptial flights followed by dispersal of the fertilized foundresses. Some ant species, however, form large polygynous supercolonies, in which mating takes place within the maternal nest (intranidal mating) and fertilized queens disperse within or along the boundary of the supercolony, leading to supercolony growth (colony budding). As a consequence, gene flow is largely confined within supercolonies. Over time, such supercolonies may diverge genetically and, thus, also in recognition cues (cuticular hydrocarbons, CHC's) by a combination of genetic drift and accumulation of colony-specific, neutral mutations. Methodology/Principal Findings: We tested this hypothesis for six supercolonies of the invasive ant Anoplolepis gracilipes in north-east Borneo. Within supercolonies, workers from different nests tolerated each other, were closely related and showed highly similar CHC profiles. Between supercolonies, aggression ranged from tolerance to mortal encounters and was negatively correlated with relatedness and CHC profile similarity. Supercolonies were genetically and chemically distinct, with mutually aggressive supercolony pairs sharing only 33.1\%617.5\% (mean 6 SD) of their alleles across six microsatellite loci and 73.8\%611.6\% of the compounds in their CHC profile. Moreover, the proportion of alleles that differed between supercolony pairs was positively correlated to the proportion of qualitatively different CHC compounds. These qualitatively differing CHC compounds were found across various substance classes including alkanes, alkenes and mono-, di- and trimethyl-branched alkanes. Conclusions: We conclude that positive feedback between genetic, chemical and behavioural traits may further enhance supercolony differentiation through genetic drift and neutral evolution, and may drive colonies towards different evolutionary pathways, possibly including speciation.}, subject = {Ameisen}, language = {en} } @article{MenzelBluethgenTolaschetal.2013, author = {Menzel, Florian and Bl{\"u}thgen, Nico and Tolasch, Till and Conrad, J{\"u}rgen and Beifuss, Uwe and Beuerle, Till and Schmitt, Thomas}, title = {Crematoenones - a novel substance class exhibited by ants functions as appeasement signal}, series = {Frontiers in Zoology}, volume = {10}, journal = {Frontiers in Zoology}, number = {32}, issn = {1742-9994}, doi = {10.1186/1742-9994-10-32}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-122595}, year = {2013}, abstract = {Background: Parasitic, commensalistic, and mutualistic guests in social insect colonies often circumvent their hosts' nestmate recognition system to be accepted. These tolerance strategies include chemical mimicry and chemical insignificance. While tolerance strategies have been studied intensively in social parasites, little is known about these mechanisms in non-parasitic interactions. Here, we describe a strategy used in a parabiotic association, i.e. two mutualistic ant species that regularly share a common nest although they have overlapping food niches. One of them, Crematogaster modiglianii, produces an array of cuticular compounds which represent a substance class undescribed in nature so far. They occur in high abundances, which suggests an important function in the ant's association with its partner Camponotus rufifemur. Results: We elucidated the structure of one of the main compounds from cuticular extracts using gas chromatography, mass spectrometry, chemical derivatizations and nuclear magnetic resonance spectroscopy (NMR). The compound consists of two fused six-membered rings with two alkyl groups, one of which carries a keto functionality. To our knowledge, this is the first report on the identification of this substance class in nature. We suggest naming the compound crematoenone. In behavioural assays, crematoenones reduced interspecific aggression. Camponotus showed less aggression to allospecific cuticular hydrocarbons when combined with crematoenones. Thus, they function as appeasement substances. However, although the crematoenone composition was highly colony-specific, interspecific recognition was mediated by cuticular hydrocarbons, and not by crematoenones. Conclusions: Crematenones enable Crematogaster to evade Camponotus aggression, and thus reduce potential costs from competition with Camponotus. Hence, they seem to be a key factor in the parabiosis, and help Crematogaster to gain a net benefit from the association and thus maintain a mutualistic association over evolutionary time. To our knowledge, putative appeasement substances have been reported only once so far, and never between non-parasitic species. Since most organisms associated with social insects need to overcome their nestmate recognition system, we hypothesize that appeasement substances might play an important role in the evolution and maintenance of other mutualistic associations as well, by allowing organisms to reduce costs from antagonistic behaviour of other species.}, language = {en} } @article{RoedelBredeHirschfeldetal.2013, author = {R{\"o}del, Mark-Oliver and Brede, Christian and Hirschfeld, Mareike and Schmitt, Thomas and Favreau, Philippe and St{\"o}cklin, Reto and Wunder, Cora and Mebs, Dietrich}, title = {Chemical Camouflage - A Frog's Strategy to Co-Exist with Aggressive Ants}, series = {PLOS ONE}, volume = {8}, journal = {PLOS ONE}, number = {12}, issn = {1932-6203}, doi = {10.1371/journal.pone.0081950}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-128181}, pages = {e81950}, year = {2013}, abstract = {Whereas interspecific associations receive considerable attention in evolutionary, behavioural and ecological literature, the proximate bases for these associations are usually unknown. This in particular applies to associations between vertebrates with invertebrates. The West-African savanna frog Phrynomantis microps lives in the underground nest of ponerine ants (Paltothyreus tarsatus). The ants usually react highly aggressively when disturbed by fiercely stinging, but the frog is not attacked and lives unharmed among the ants. Herein we examined the proximate mechanisms for this unusual association. Experiments with termites and mealworms covered with the skin secretion of the frog revealed that specific chemical compounds seem to prevent the ants from stinging. By HPLC-fractionation of an aqueous solution of the frogs' skin secretion, two peptides of 1,029 and 1,143 Da were isolated and found to inhibit the aggressive behaviour of the ants. By de novo sequencing using tandem mass spectrometry, the amino acid sequence of both peptides consisting of a chain of 9 and 11 residues, respectively, was elucidated. Both peptides were synthesized and tested, and exhibited the same inhibitory properties as the original frog secretions. These novel peptides most likely act as an appeasement allomone and may serve as models for taming insect aggression.}, language = {en} } @article{StrubeBlossBrownSpaetheetal.2015, author = {Strube-Bloss, Martin F. and Brown, Austin and Spaethe, Johannes and Schmitt, Thomas and R{\"o}ssler, Wolfgang}, title = {Extracting the Behaviorally Relevant Stimulus: Unique Neural Representation of Farnesol, a Component of the Recruitment Pheromone of Bombus terrestris}, series = {PLoS One}, volume = {10}, journal = {PLoS One}, number = {9}, doi = {10.1371/journal.pone.0137413}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-125875}, pages = {e0137413}, year = {2015}, abstract = {To trigger innate behavior, sensory neural networks are pre-tuned to extract biologically relevant stimuli. Many male-female or insect-plant interactions depend on this phenomenon. Especially communication among individuals within social groups depends on innate behaviors. One example is the efficient recruitment of nest mates by successful bumblebee foragers. Returning foragers release a recruitment pheromone in the nest while they perform a 'dance' behavior to activate unemployed nest mates. A major component of this pheromone is the sesquiterpenoid farnesol. How farnesol is processed and perceived by the olfactory system, has not yet been identified. It is much likely that processing farnesol involves an innate mechanism for the extraction of relevant information to trigger a fast and reliable behavioral response. To test this hypothesis, we used population response analyses of 100 antennal lobe (AL) neurons recorded in alive bumblebee workers under repeated stimulation with four behaviorally different, but chemically related odorants (geraniol, citronellol, citronellal and farnesol). The analysis identified a unique neural representation of the recruitment pheromone component compared to the other odorants that are predominantly emitted by flowers. The farnesol induced population activity in the AL allowed a reliable separation of farnesol from all other chemically related odor stimuli we tested. We conclude that the farnesol induced population activity may reflect a predetermined representation within the AL-neural network allowing efficient and fast extraction of a behaviorally relevant stimulus. Furthermore, the results show that population response analyses of multiple single AL-units may provide a powerful tool to identify distinct representations of behaviorally relevant odors.}, language = {en} } @phdthesis{Schmitt2004, author = {Schmitt, Thomas}, title = {Communication in the hymenoptera : chemistry, ecology and evolution}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-11267}, school = {Universit{\"a}t W{\"u}rzburg}, year = {2004}, abstract = {Insects exhibit complex systems of communication with chemical signalling being the most important mode. Although there are many studies on chemical communication in insects, the evolution of chemical signals is not well understood. Due to the conflict of interests between individuals, different selective pressures might act on sender and receiver. In this thesis I investigate different types of communication where either the sender, the receiver or both parties yield benefits. These studies were conducted with one digger wasp species, honeybees, one chrysidid wasp, and three ant species. Senders might benefit by exploiting existing preferences of receivers. Such sensory exploitation might influence the evolution of male signals that are designed to attract females. The sex pheromone of male European beewolves Philanthus triangulum (Hymenoptera, Crabronidae) might have evolved according to the sensory exploitation hypothesis. A three-step scenario is supported by our studies. First, a major component of the honeybee alarm pheromone, (Z)-11-eicosen-1-ol, is also found on the cuticles and in the air surrounding foraging honeybees. Second, it could be shown, that (Z)-11- eicosen-1-ol plays a crucial role as kairomone for prey identification of honeybees by beewolf females. Third, a reanalysis of the beewolf male sex pheromone shows a remarkable similarity of compounds between the pheromone and the honeybee cuticle, besides the co-occurrence of (Z)-11-eisosen-ol. The majority of the cuticular hydrocarbons of honeybees occur also in the headspace of foraging workers. These results strongly support the hypothesis that beewolf males evolved a pheromone that exploits the females' pre-existing sensory sensitivity. In addition, the male sex pheromone shows a significantly higher similarity among brothers than among non-related individuals, which might enable beewolf females to discriminate against brothers and avoid detrimental effects of breeding. Together with the studies on the possible sensory exploitation this result shows that both, male and female beewolves probably gain more benefits than costs from the pheromone communication and, thus, the communication system as a whole can be regarded as cooperative. To maintain the reproductive division of labour in eusocial colonies, queens have to signal their presence and fecundity. In the ant Camponotus floridanus (Hymenoptera, Formicidae) queens mark their own eggs with a distinctive pattern of cuticular hydrocarbons. Two different hypotheses have been developed. One suggests a form of worker manipulation by the queen. The alternative hypothesis assumes a cooperative signal that provides information on the condition of the queen. The results of our investigation clearly favour the latter hypothesis. Chemical mimicry is a form of non-cooperative communication that benefits predominantly the sender. We provided conclusive evidence that the cockoo wasp, Hedychrum rutilans (Hymenoptera, Chrysididae), the primary brood parasitoid of Philanthus triangulum, evades recognition by beewolf females most probably by chemical mimicry of the odour of its host. Furthermore, the adaptation of the chemical signature in the social ant parasite Protomognathus americanus (Hymenoptera, Formicidae) to its Leptothorax (Hymenoptera, Formicidae) hosts was investigated. Although this parasite is principally adapted to its hosts' cuticular hydrocarbon profile, there are still pronounced differences between the profiles of parasites and hosts. This might be explained by the trade-off, which the parasites faces when confronted locally with two host species with different cuticular hydrocarbon profiles. Non-cooperative communication in the sense that only receivers benefit was discovered in the exploitation of honeybees volatile cuticular hydrocarbons by beewolf females. By using emitted (Z)-11-eicosen-1-ol as a kairomone, the receiver, the beewolf female, yields the benefits and the sender, the honeybee prey, bears all the costs. The results of these studies contribute to the understanding of the evolution of cooperative and non-cooperative communication with chemical signals taking into account differential benefits for sender and/or receiver.}, subject = {Hautfl{\"u}gler}, language = {en} } @article{ChristopherDUgelvigWiesenhoferetal.2018, author = {Christopher D., Pull and Ugelvig, Line V. and Wiesenhofer, Florian and Anna V., Grasse and Tragust, Simon and Schmitt, Thomas and Brown, Mark JF and Cremer, Sylvia}, title = {Destructive disinfection of infected brood prevents systemic disease spread in ant colonies}, series = {eLIFE}, volume = {7}, journal = {eLIFE}, doi = {10.7554/eLife.32073}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-223728}, pages = {e 32073, 1-29}, year = {2018}, abstract = {In social groups, infections have the potential to spread rapidly and cause disease outbreaks. Here, we show that in a social insect, the ant Lasius neglectus, the negative consequences of fungal infections (Metarhizium brunneum) can be mitigated by employing an efficient multicomponent behaviour, termed destructive disinfection, which prevents further spread of the disease through the colony. Ants specifically target infected pupae during the pathogens non-contagious incubation period, utilising chemical 'sickness cues' emitted by pupae. They then remove the pupal cocoon, perforate its cuticle and administer antimicrobial poison, which enters the body and prevents pathogen replication from the inside out. Like the immune system of a metazoan body that specifically targets and eliminates infected cells, ants destroy infected brood to stop the pathogen completing its lifecycle, thus protecting the rest of the colony. Hence, in an analogous fashion, the same principles of disease defence apply at different levels of biological organisation.}, language = {en} } @article{MaihoffBohlkeBrockmannetal.2022, author = {Maihoff, Fabienne and Bohlke, Kyte and Brockmann, Axel and Schmitt, Thomas}, title = {Increased complexity of worker CHC profiles in Apis dorsata correlates with nesting ecology}, series = {PLoS ONE}, volume = {17}, journal = {PLoS ONE}, number = {7}, doi = {10.1371/journal.pone.0271745}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-301353}, year = {2022}, abstract = {Cuticular hydrocarbons (CHC) are known to serve as discrimination cues and will trigger defence behaviour in a plethora of eusocial insects. However, little is known how about nestmate recognition ability selects for CHC diversification. In this study we investigate differences in CHC composition of four major honey bee species with respect to the differences in their nesting behavior. In contrast to A. mellifera, A. cerana and A. florea, the giant honey bee A. dorsata prefers to build their nests in aggregations with very small spatial distances between nests, which increases the probability of intrusions. Thus, A. dorsata exhibits a particularly challenging nesting behavior which we hypothesize should be accompanied with an improved nestmate recognition system. Comparative analyses of the worker CHC profiles indicate that A. dorsata workers exhibit a unique and more complex CHC profile than the other three honey bee species. This increased complexity is likely based on a developmental process that retains the capability to synthesize methyl-branched hydrocarbons as adults. Furthermore, two sets of behavioral experiments provide evidence that A. dorsata shows an improved nestmate discrimination ability compared to the phylogenetically ancestral A. florea, which is also open-nesting but does not form nest aggregations. The results of our study suggest that ecological traits like nesting in aggregation might be able to drive CHC profile diversification even in closely related insect species.}, language = {en} } @techreport{MuellerSchererLorenzenAmmeretal.2022, author = {M{\"u}ller, J{\"o}rg and Scherer-Lorenzen, Michael and Ammer, Christian and Eisenhauer, Nico and Seidel, Dominik and Schuldt, Bernhard and Biedermann, Peter and Schmitt, Thomas and K{\"u}nzer, Claudia and Wegmann, Martin and Cesarz, Simone and Peters, Marcell and Feldhaar, Heike and Steffan-Dewenter, Ingolf and Claßen, Alice and B{\"a}ssler, Claus and von Oheimb, Goddert and Fichtner, Andreas and Thorn, Simon and Weisser, Wolfgang}, title = {BETA-FOR: Erh{\"o}hung der strukturellen Diversit{\"a}t zwischen Waldbest{\"a}nden zur Erh{\"o}hung der Multidiversit{\"a}t und Multifunktionalit{\"a}t in Produktionsw{\"a}ldern. Antragstext f{\"u}r die DFG Forschungsgruppe FOR 5375}, doi = {10.25972/OPUS-29084}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-290849}, pages = {210}, year = {2022}, abstract = {Der in j{\"u}ngster Zeit beobachtete kontinuierliche Verlust der β-Diversit{\"a}t in {\"O}kosystemen deutet auf homogene Gemeinschaften auf Landschaftsebene hin, was haupts{\"a}chlich auf die steigende Landnutzungsintensit{\"a}t zur{\"u}ckgef{\"u}hrt wird. Biologische Vielfalt ist mit zahlreichen Funktionen und der Stabilit{\"a}t von {\"O}kosystemen verkn{\"u}pft. Es ist daher zu erwarten, dass eine abnehmende β-Diversit{\"a}t auch die Multifunktionalit{\"a}t verringert. Wir kombinieren hier Fachwissen aus der Forstwissenschaft, der {\"O}kologie, der Fernerkundung, der chemischen {\"O}kologie und der Statistik in einem gemeinschaftlichen und experimentellen β-Diversit{\"a}tsdesign, um einerseits die Auswirkungen der Homogenisierung zu bewerten und andererseits Konzepte zu entwickeln, um negative Auswirkungen durch Homogenisierung in W{\"a}ldern r{\"u}ckg{\"a}ngig zu machen. Konkret werden wir uns mit der Frage besch{\"a}ftigen, ob die Verbesserung der strukturellen β-Komplexit{\"a}t (ESBC) in W{\"a}ldern durch Waldbau oder nat{\"u}rliche St{\"o}rungen die Biodiversit{\"a}t und Multifunktionalit{\"a}t in ehemals homogenen Produktionsw{\"a}ldern erh{\"o}hen kann. Unser Ansatz wird m{\"o}gliche Mechanismen hinter den beobachteten Homogenisierungs-Diversit{\"a}ts-Beziehungen identifizieren und zeigen, wie sich diese auf die Multifunktionalit{\"a}t auswirken. An elf Standorten in ganz Deutschland haben wir dazu zwei Waldbest{\"a}nde als zwei kleine "Waldlandschaften" ausgew{\"a}hlt. In einem dieser beiden Best{\"a}nde haben wir ESBC (Enhancement of Structural Beta Complexity)-Behandlungen durchgef{\"u}hrt. Im zweiten, dem Kontrollbestand, werden wir die gleich Anzahl 50x50m Parzellen ohne ESBC einrichten. Auf allen Parzellen werden wir 18 taxonomische Artengruppen aller trophischer Ebenen und 21 {\"O}kosystemfunktionen, einschließlich der wichtigsten Funktionen in W{\"a}ldern der gem{\"a}ßigten Zonen, messen. Der statistische Rahmen wird eine umfassende Analyse der Biodiversit{\"a}t erm{\"o}glichen, indem verschiedenen Aspekte (taxonomische, funktionelle und phylogenetische Vielfalt) auf verschiedenen Skalenebenen (α-, β-, γ-Diversit{\"a}t) quantifiziert werden. Um die Gesamtdiversit{\"a}t zu kombinieren, werden wir das Konzept der Multidiversit{\"a}t auf die 18 Taxa anwenden. Wir werden neue Ans{\"a}tze zur Quantifizierung und Aufteilung der Multifunktionalit{\"a}t auf α- und β-Skalen verwenden und entwickeln. Durch die experimentelle Beschreibung des Zusammenhangs zwischen β-Diversit{\"a}t und Multifunktionalit{\"a}t in einer Reallandschaft wird unsere Forschung einen neuen Weg einschlagen. Dar{\"u}ber hinaus werden wir dazu beitragen, verbesserte Leitlinien f{\"u}r waldbauliche Konzepte und f{\"u}r das Management nat{\"u}rlicher St{\"o}rungen zu entwickeln, um Homogenisierungseffekte der Vergangenheit umzukehren.}, subject = {Wald{\"o}kosystem}, language = {en} }