@article{WeigelMeyerSebald1989, author = {Weigel, U. and Meyer, M. and Sebald, Walter}, title = {Mutant proteins of human interleukin 2. Renaturation yield, proliferative activity and receptor binding}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62543}, year = {1989}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{FlueggeFischerGrossetal.1989, author = {Fl{\"u}gge, U. I. and Fischer, K. and Gross, A. and Sebald, Walter and Lottspeich, F. and Eckerskorn, C.}, title = {The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62559}, year = {1989}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{WeichSebaldSchaireretal.1986, author = {Weich, H. A. and Sebald, Walter and Schairer, H. U. and Hoppe, J.}, title = {The human osteosarcoma cell line U-2 OS expresses a 3.8 kilobase mRNA which codes for the sequence of the PDGF-B chain}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62588}, year = {1986}, abstract = {A cDNA clone of about 2500 basepairswas prepared from the human osteosarcoma cellline U-2 OS by hybridizing with a v-sis probe. Sequence analysis showed that this cDNA contains the coding region for the PDGF-B chain. Here we report that the mitogen secreted by these osteosarcoma cells contains the PDGF-B chain and is probably a homodimer of two B-chains.}, subject = {Biochemie}, language = {en} } @article{HoppeFriedlSchaireretal.1983, author = {Hoppe, J. and Friedl, P. and Schairer, H. U. and Sebald, Walter and Meyenburg, K. von and Jorgensen, B. B.}, title = {The topology of the proton translocating F\(_0\) component of the ATP synthase from E. coli K12: studies with proteases}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62718}, year = {1983}, abstract = {The accessibility of the three F\(_0\) subunits a, b and c from the Escherichia coli Kll A TP synthase to various proteases was studied in F\(_1\)-depleted inverted membrane vesicles. Subunit b was very sensitive to all applied proteases. Chymotrypsin produced a defined fragment of mol. wt. 1S 000 which remained tightly bound to the membrane. The cleavage site was located at the C-terminal region of subunit b. Larger amounts of proteases were necessary to attack subunit a (mol. wt. 30 000). There was no detectable deavage of subunit c. It is suggested that the major hydrophilic part of subunit b extends from the membrane into the cytoplasm and is in contact with the F\(_1\) sector. The F\(_1\) sector was found to afford some protection against proteolysis oftheb subunit in vitro andin vivo. Protease digestion bad no influence on the electro-impelled H\(^+\) conduction via F\(_0\) bot ATP-dependent H\(^+\) translocation could not be reconstituted upon binding of F\(_1\)• A possible role for subunit b as a linker between catalytic events on the F\(_1\) component and the proton pathway across the membrane is discussed.}, subject = {Biochemie}, language = {en} } @article{SchairerHoppeSebaldetal.1982, author = {Schairer, H. U. and Hoppe, J. and Sebald, Walter and Friedl, P.}, title = {Topological and functional aspects of the proton conductor, F\(_0\), of the Escherichia coli ATP-synthase}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62721}, year = {1982}, abstract = {The isolated H\(^+\) conductor, F\(_0\) , of the Escherichia co1i ATP-synthase consists of three subunits, a, b, and c. H\(^+\) -permeable liposomes can be reconstit~ted with F\(_0\) and lipids; addition of F\(_1\)-ATPase reconstitutes a functional ATP-synthase. Mutants with altered or misslng F\(_0\) subunits are defective in H\(^+\) conduction. Thus, all three subunits are necessary for the expression of H\(^+\) conduction. The subunits a and b contain binding sites for F\(_1\)• Computer calculations, cross-links, membrane-permeating photo-reactive labels, and proteases were used to develop tentative structural models for the individual F\(_0\) subunits.}, subject = {Biochemie}, language = {en} } @article{SebaldFriedlSchaireretal.1982, author = {Sebald, Walter and Friedl, P. and Schairer, H. U. and Hoppe, J.}, title = {Structure and genetics of the H\(^+\)-conducting F\(_0\) portion of the ATP synthase}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62733}, year = {1982}, abstract = {The ATP synthase occurs in remarkably conserved form in procaryotic and eucaryotic cells. Thus, our present knowledge of ATP synthase is derived from sturlies of the enzyme from different organisms, each affering specific experimental possibilities. In recent tim es, research on the H\(^+\) -conducting F0 part of the ATP synthase has been greatly stimulated by two developments in the Escherichio coli system. Firstly, the purification and reconstitution of the whole ATP synthase as weil as the proton conductor Fa from E. coli have been achieved. These functionally active preparations are well defined in terms of subunit composition, similar to the thermophilic enzyme from PS-3 studied by Kagawa's group.u Secondly, the genetics and the molecular cloning of the genes of all the F\(_0\) subunits from E. coli yielded information on the function of subunit polypeptides and essential amino acid residues. Furthermore, the amino acid sequence of hydrophobic F\(_0\) subunits, which are difficult to analyze by protein-chemical techniques, could be derived from the nucleotide sequence of the genes. These achievements, which shall be briefly summarized in the next part of this communication, provide the framework to study specific aspects of the structure and function of the F\(_0\) subunits.}, subject = {Biochemie}, language = {en} } @article{HarnischWeissSebald1985, author = {Harnisch, U. and Weiss, H. and Sebald, Walter}, title = {The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62631}, year = {1985}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{GabelliniHarnischMcCarthyetal.1985, author = {Gabellini, N. and Harnisch, U. and McCarthy, J. E. and Hauska, G. and Sebald, Walter}, title = {Cloning and expression of the fbc operon encoding the FeS protein, cytochrome b and cytochrome c\(_1\) from the Rhodopseudomonas sphaeroides b/c\(_1\) complex}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62642}, year = {1985}, abstract = {The gene for the FeS protein of the Rhodopseudomonas sphaeroides b/c1 complex was identified by means of crosshybridization with a segment of the gene encoding the corresponding FeS protein of Neurospora crassa. Plasmids (pRSF1-14) containing the cross-hybridizing region, covering in total 13.5 kb of chromosomal DNA, were expressed in vitro in a homologous system. One RSF plasmid directed the synthesis of all three main polypeptides of the R. sphaeroides blc1 complex: the FeS protein, cytochrome b and cytochrome c1• The FeS protein and cytochrome c1 were apparently synthesized as precursor fonns. None of the pRSF plasmids directed the synthesis of the 10-kd polypeptide found in b/c1 complex preparations. Partial sequencing of the cloned region was performed. Several sites of strong homology between R. sphaeroides and eukaryotic polypeptides of the b/c1 complex were identified. The genes encode the three b/c1 polypeptides in the order: (5') FeS protein, cytochrome b, cytochrome c1• The three genes are transcribed to give a polycistronic mRNA of 2.9 kb. This transcriptional unit has been designated the jbc operon; its coding capacity corresponds to the size of the polycistronic mRNA assuming that only the genes for the FeS protein (jbcF), cytochrome b (jbcß) and cytochrome c1 (jbcC) are present. This could indicate that these three subunits constitute the minimal catalytic unit of the b/c1 complex from photosynthetic membranes.}, subject = {Biochemie}, language = {en} } @article{McCarthySchairerSebald1985, author = {McCarthy, J. E. and Schairer, H. U. and Sebald, Walter}, title = {Translational initiation frequency of atp genes from Escherichia coli: identification of an intercistronic sequence that enhances translation}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62657}, year = {1985}, abstract = {The c, b and {\"o} subunit genes of the Escherichia coli atp operon were cloned individually in an expression vector between the tac fusion promoter and the galK gene. The relative rates of subunit synthesis directed by the cloned genes were similar in vitro andin vivo and compared favourably with the subunit stoichiometry of the assembled proton-translocating A TP synthase of E. coli in vivo. The rate of synthesis of subunit c was at least six times that of subunit b and 18 times that of subunit {\"o}. Progressive shortening of the long intercistronic sequence lying upstream of the subunit c gene showed that maximal expression of this gene is dependent upon the presence of a sequence stretching > 20 bp upstream of the Shine-Dalgarno site. This sequence thus acts to enhance the rate of translational initiation. The possibility that similar sequences might perform the same function in other operons of E. coli and bacteriophage A is also discussed. Translation of the subunit b cistron is partially coupled to translation of the preceding subunit c cistron. In conclusion, the expression of all the atp operon genes could be adjusted to accommodate the subunit requirements of A TP synthase assembly primarily by means of mechanisms which control the efficiency of translational initiation and re-initiation at the respective cistron start codons.}, subject = {Biochemie}, language = {en} } @article{HoppeSchairerSebald1980, author = {Hoppe, J. and Schairer, H. U. and Sebald, Walter}, title = {The proteolipid of a mutant ATPase from Escherichia coli defective in H\(^+\)-conduction contains a glycine instead of the carbodiimide-reactive aspartyl residue}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62769}, year = {1980}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{SchneiderSchauliesSchneiderSchauliesBrinkmannetal.1992, author = {Schneider-Schaulies, J{\"u}rgen and Schneider-Schaulies, Sibylle and Brinkmann, R. and Tas, P. and Halbr{\"u}gge, M. and Walter, U. and Holmes, H.C. and ter Meulen, Volker}, title = {HIV-1 gp120 receptor on CD4-negative brain cells activates a tyrosine kinase}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54872}, year = {1992}, abstract = {Human immunodeficiency virus (HIV-1) infection in the human brain Ieads to characteristic neuropathological changes, which may result indirectly from interactions of the envelope glycoprotein gp 120 with neurons and/or glial cells. We therefore investigated the binding of recombinant gp120 (rgp120) to human neural cells and its effect on int~acellular.s.ignallin~. Herewe pre~ent evidence that rgp120, besides binding to galactocerebroside or galactosyl-sulfatlde, spec1f1cally bmds to a protem receptor of a relative molecular mass of approximately 180,000 Da (180 kDa) pre~ent. on the CD4-negative glioma cells D-54, but not on Molt4 T lymphocytes. Binding of rgp120 to this receptor rap1dly 1nduced a tyrosine-specific protein kinase activity leading to tyrosine phosphorylation of 130- and 115-kDa p~oteins. The c~ncentration of intracellular calciumwas not affected by rgp120 in these cells. Our data suggest a novel Signal transduc1ng HIV-1 gp120 receptor on CD4-negative glial cells, which may contribute to the neuropathological changes observed in HIV-1-infected brains.}, subject = {Immunologie}, language = {en} } @article{RukoyatkinaMindukshevWalteretal.2013, author = {Rukoyatkina, N. and Mindukshev, I. and Walter, U. and Gambaryan, S.}, title = {Dual role of the p38 \(MAPK/cPLA_2\) pathway in the regulation of platelet apoptosis induced by ABT-737 and strong platelet agonists}, series = {Cell Death \& Disease}, volume = {4}, journal = {Cell Death \& Disease}, number = {e931}, doi = {10.1038/cddis.2013.459}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-128783}, year = {2013}, abstract = {p38 Mitogen-activated protein (MAP) kinase is involved in the apoptosis of nucleated cells. Although platelets are anucleated cells, apoptotic proteins have been shown to regulate platelet lifespan. However, the involvement of p38 MAP kinase in platelet apoptosis is not yet clearly defined. Therefore, we investigated the role of p38 MAP kinase in apoptosis induced by a mimetic of BH3-only proteins, ABT-737, and in apoptosis-like events induced by such strong platelet agonists as thrombin in combination with convulxin (Thr/Cvx), both of which result in p38 MAP kinase phosphorylation and activation. A p38 inhibitor (SB202190) inhibited the apoptotic events induced by ABT-737 but did not influence those induced by Thr/Cvx. The inhibitor also reduced the phosphorylation of cytosolic phospholipase \(A_2\) (cPLA2), an established p38 substrate, induced by ABT-737 or Thr/Cvx. ABT-737, but not Thr/Cvx, induced the caspase 3-dependent cleavage and inactivation of cPLA2. Thus, p38 MAPK promotes ABT-737-induced apoptosis by inhibiting the cPLA2/arachidonate pathway. We also show that arachidonic acid (AA) itself and in combination with Thr/Cvx or ABT-737 at low concentrations prevented apoptotic events, whereas at high concentrations it enhanced such events. Our data support the hypothesis that the p38 MAPK-triggered arachidonate pathway serves as a defense mechanism against apoptosis under physiological conditions.}, language = {en} } @article{WiedmannHeuschmannHillmannetal.2014, author = {Wiedmann, Silke and Heuschmann, Peter U. and Hillmann, Steffi and Busse, Otto and Wiethoelter, Horst and Walter, Georg M. and Seidel, Guenter and Misselwitz, Bjoern and Janssen, Alfred and Berger, Klaus and Burmeister, Christoph and Matthias, Christine and Kolominsky-Rabas, Peter and Hermanek, Peter}, title = {The Quality of Acute Stroke Care-an Analysis of Evidence-Based Indicators in 260 000 Patients}, series = {Deutsches {\"A}rzteblatt International}, volume = {111}, journal = {Deutsches {\"A}rzteblatt International}, number = {45}, issn = {1866-0452}, doi = {10.3238/arztebl.2014.0759}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-114747}, pages = {759-765}, year = {2014}, abstract = {Background: Stroke patients should be cared for in accordance with evidence-based guidelines. The extent of implementation of guidelines for the acute care of stroke patients in Germany has been unclear to date. Methods: The regional quality assurance projects that cooperate in the framework of the German Stroke Registers Study Group (Arbeitsgemeinschaft Deutscher Schlaganfall-Register, ADSR) collected data on the care of stroke patients in 627 hospitals in 2012. The quality of the acute hospital care of patients with stroke or transient ischemic attack (TIA) was assessed on the basis of 15 standardized, evidence-based quality indicators and compared across the nine participating regional quality assurance projects. Results: Data were obtained on more than 260 000 patients nationwide. Intravenous thrombolysis was performed in 59.7\% of eligible ischemic stroke patients patients (range among participating projects, 49.7-63.6\%). Dysphagia screening was documented in 86.2\% (range, 74.8-93.1\%). For the following indicators, the defined targets were not reached for all of Germany: antiaggregation within 48 hours, 93.4\% (range, 86.6-96.4\%); anticoagulation for atrial fibrillation, 77.6\% (range, 72.4-80.1\%); standardized dysphagia screening, 86.2\% (range, 74.8-93.1\%); oral and written information of the patients or their relatives, 86.1\% (range, 75.4-91.5\%). The rate of patients examined or treated by a speech therapist was in the target range. Conclusion: The defined targets were reached for most of the quality indicators. Some indicators, however, varied widely across regional quality assurance projects. This implies that the standardization of care for stroke patients in Germany has not yet been fully achieved.}, language = {en} }