@article{VollmuthSchlickerGuoetal.2022,
  author    = {Vollmuth, Nadine and Schlicker, Lisa and Guo, Yongxia and Hovhannisyan, Pargev and Janaki-Raman, Sudha and Kurmasheva, Naziia and Schmitz, Werner and Schulze, Almut and Stelzner, Kathrin and Rajeeve, Karthika and Rudel, Thomas},
  title     = {c-Myc plays a key role in IFN-γ-induced persistence of Chlamydia trachomatis},
  series = {eLife},
  volume    = {11},
  journal   = {eLife},
  doi       = {10.7554/eLife.76721},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-301385},
  year      = {2022},
  abstract  = {Chlamydia trachomatis (Ctr) can persist over extended times within their host cell and thereby establish chronic infections. One of the major inducers of chlamydial persistence is interferon-gamma (IFN-γ) released by immune cells as a mechanism of immune defence. IFN-γ activates the catabolic depletion of L-tryptophan (Trp) via indoleamine-2,3-dioxygenase (IDO), resulting in persistent Ctr. Here, we show that IFN-γ induces the downregulation of c-Myc, the key regulator of host cell metabolism, in a STAT1-dependent manner. Expression of c-Myc rescued Ctr from IFN-γ-induced persistence in cell lines and human fallopian tube organoids. Trp concentrations control c-Myc levels most likely via the PI3K-GSK3β axis. Unbiased metabolic analysis revealed that Ctr infection reprograms the host cell tricarboxylic acid (TCA) cycle to support pyrimidine biosynthesis. Addition of TCA cycle intermediates or pyrimidine/purine nucleosides to infected cells rescued Ctr from IFN-γ-induced persistence. Thus, our results challenge the longstanding hypothesis of Trp depletion through IDO as the major mechanism of IFN-γ-induced metabolic immune defence and significantly extends the understanding of the role of IFN-γ as a broad modulator of host cell metabolism.},
  language  = {en}
}
@article{PrantlZeckBaueretal.2022,
  author    = {Prantl, Thomas and Zeck, Timo and Bauer, Andre and Ten, Peter and Prantl, Dominik and Yahya, Ala Eddine Ben and Ifflaender, Lukas and Dmitrienko, Alexandra and Krupitzer, Christian and Kounev, Samuel},
  title     = {A Survey on Secure Group Communication Schemes With Focus on IoT Communication},
  series = {IEEE Access},
  volume    = {10},
  journal   = {IEEE Access},
  doi       = {10.1109/ACCESS.2022.3206451},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-300257},
  pages     = {99944 -- 99962},
  year      = {2022},
  abstract  = {A key feature for Internet of Things (IoT) is to control what content is available to each user. To handle this access management, encryption schemes can be used. Due to the diverse usage of encryption schemes, there are various realizations of 1-to-1, 1-to-n, and n-to-n schemes in the literature. This multitude of encryption methods with a wide variety of properties presents developers with the challenge of selecting the optimal method for a particular use case, which is further complicated by the fact that there is no overview of existing encryption schemes. To fill this gap, we envision a cryptography encyclopedia providing such an overview of existing encryption schemes. In this survey paper, we take a first step towards such an encyclopedia by creating a sub-encyclopedia for secure group communication (SGC) schemes, which belong to the n-to-n category. We extensively surveyed the state-of-the-art and classified 47 different schemes. More precisely, we provide (i) a comprehensive overview of the relevant security features, (ii) a set of relevant performance metrics, (iii) a classification for secure group communication schemes, and (iv) workflow descriptions of the 47 schemes. Moreover, we perform a detailed performance and security evaluation of the 47 secure group communication schemes. Based on this evaluation, we create a guideline for the selection of secure group communication schemes.},
  language  = {en}
}
@article{GriesbeckMichailRauchetal.2019,
  author    = {Griesbeck, Stefanie and Michail, Evripidis and Rauch, Florian and Ogasawara, Hiroaki and Wang, Chenguang and Sato, Yoshikatsu and Edkins, Robert M. and Zhang, Zuolun and Taki, Masayasu and Lambert, Christoph and Yamaguchi, Shigehiro and Marder, Todd B.},
  title     = {The Effect of Branching on One- and Two-Photon Absorption, Cell Viability and Localization of Cationic Triarylborane Chromophores with Dipolar versus Octupolar Charge Distributions for Cellular Imaging},
  series = {Chemistry - A European Journal},
  volume    = {25},
  journal   = {Chemistry - A European Journal},
  number    = {57},
  doi       = {10.1002/chem.201902461},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-204829},
  pages     = {13164-13175},
  year      = {2019},
  abstract  = {Two different chromophores, namely a dipolar and an octupolar system, were prepared and their linear and nonlinear optical properties as well as their bioimaging capabilities were compared. Both contain triphenylamine as the donor and a triarylborane as the acceptor, the latter modified with cationic trimethylammonio groups to provide solubility in aqueous media. The octupolar system exhibits a much higher two-photon brightness, and also better cell viability and enhanced selectivity for lysosomes compared with the dipolar chromophore. Furthermore, both dyes were applied in two-photon excited fluorescence (TPEF) live-cell imaging.},
  language  = {en}
}
@article{DresenPimientoPateletal.2023,
  author    = {Dresen, Ellen and Pimiento, Jose M. and Patel, Jayshil J. and Heyland, Daren K. and Rice, Todd W. and Stoppe, Christian},
  title     = {Overview of oxidative stress and the role of micronutrients in critical illness},
  series = {Journal of Parenteral and Enteral Nutrition},
  volume    = {47},
  journal   = {Journal of Parenteral and Enteral Nutrition},
  doi       = {10.1002/jpen.2421},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-318186},
  pages     = {S38 -- S49},
  year      = {2023},
  abstract  = {Inflammation and oxidative stress represent physiological response mechanisms to different types of stimuli and injury during critical illness. Its proper regulation is fundamental to cellular and organismal survival and are paramount to outcomes and recovery from critical illness. A proper maintenance of the delicate balance between inflammation, oxidative stress, and immune response is crucial for resolution from critical illness with important implications for patient outcome. The extent of inflammation and oxidative stress under normal conditions is limited by the antioxidant defense system of the human body, whereas the antioxidant capacity is commonly significantly compromised, and serum levels of micronutrients and vitamins significantly depleted in patients who are critically ill. Hence, the provision of antioxidants and anti-inflammatory nutrients may help to reduce the extent of oxidative stress and therefore improve clinical outcomes in patients who are critically ill. As existing evidence of the beneficial effects of antioxidant supplementation in patients who are critically ill is still unclear, actual findings about the most promising anti-inflammatory and antioxidative candidates selenium, vitamin C, zinc, and vitamin D will be discussed in this narrative review. The existing evidence provided so far demonstrates that several factors need to be considered to determine the efficacy of an antioxidant supplementation strategy in patients who are critically ill and indicates the need for adequately designed multicenter prospective randomized control trials to evaluate the clinical significance of different types and doses of micronutrients and vitamins in selected groups of patients with different types of critical illness.},
  language  = {en}
}
@article{HurdGruebelWojciechowskietal.2021,
  author    = {Hurd, Paul J. and Gr{\"u}bel, Kornelia and Wojciechowski, Marek and Maleszka, Ryszard and R{\"o}ssler, Wolfgang},
  title     = {Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining},
  series = {Scientific Reports},
  volume    = {11},
  journal   = {Scientific Reports},
  doi       = {10.1038/s41598-021-86078-5},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-260059},
  pages     = {6852},
  year      = {2021},
  abstract  = {In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity indicates a novel previously unseen nuclear structure that does not colocalize with the cytoskeletal protein f-actin. A single rod-like assembly, 3.7-4.1 mu m long, is present in each nucleus of KCs in adult brains of worker bees and drones with the strongest immuno-labelling found in foraging bees. In brains of young queens, the labelling is more sporadic, and the rod-like structure appears to be shorter (similar to 2.1 mu m). No immunostaining is detectable in worker larvae. In pupal stage 5 during a peak of brain development only some occasional staining was identified. Although the cellular function of this unexpected structure has not been determined, the unusual distinctiveness of the revealed pattern suggests an unknown and potentially important protein assembly. One possibility is that this nuclear assembly is part of the KCs plasticity underlying the brain maturation in adult honey bees. Because no labelling with this AB is detectable in brains of the fly Drosophila melanogaster and the ant Camponotus floridanus, we tentatively named this antibody AmBNSab (Apis mellifera Brain Neurons Specific antibody). Here we report our results to make them accessible to a broader community and invite further research to unravel the biological role of this curious nuclear structure in the honey bee central brain.},
  language  = {en}
}
@article{Pirner2021,
  author    = {Pirner, Marlies},
  title     = {A review on BGK models for gas mixtures of mono and polyatomic molecules},
  series = {Fluids},
  volume    = {6},
  journal   = {Fluids},
  number    = {11},
  issn      = {2311-5521},
  doi       = {10.3390/fluids6110393},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-250161},
  year      = {2021},
  abstract  = {We consider the Bathnagar-Gross-Krook (BGK) model, an approximation of the Boltzmann equation, describing the time evolution of a single momoatomic rarefied gas and satisfying the same two main properties (conservation properties and entropy inequality). However, in practical applications, one often has to deal with two additional physical issues. First, a gas often does not consist of only one species, but it consists of a mixture of different species. Second, the particles can store energy not only in translational degrees of freedom but also in internal degrees of freedom such as rotations or vibrations (polyatomic molecules). Therefore, here, we will present recent BGK models for gas mixtures for mono- and polyatomic particles and the existing mathematical theory for these models.},
  language  = {en}
}
@article{KruegerMausKressetal.2021,
  author    = {Kr{\"u}ger, Timothy and Maus, Katharina and Kreß, Verena and Meyer-Natus, Elisabeth and Engstler, Markus},
  title     = {Single-cell motile behaviour of Trypanosoma brucei in thin-layered fluid collectives},
  series = {The European Physical Journal E},
  volume    = {44},
  journal   = {The European Physical Journal E},
  number    = {3},
  issn      = {1292-895X},
  doi       = {10.1140/epje/s10189-021-00052-7},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-273022},
  year      = {2021},
  abstract  = {We describe a system for the analysis of an important unicellular eukaryotic flagellate in a confining and crowded environment. The parasite Trypanosoma brucei is arguably one of the most versatile microswimmers known. It has unique properties as a single microswimmer and shows remarkable adaptations (not only in motility, but prominently so), to its environment during a complex developmental cycle involving two different hosts. Specific life cycle stages show fascinating collective behaviour, as millions of cells can be forced to move together in extreme confinement. Our goal is to examine such motile behaviour directly in the context of the relevant environments. Therefore, for the first time, we analyse the motility behaviour of trypanosomes directly in a widely used assay, which aims to evaluate the parasites behaviour in collectives, in response to as yet unknown parameters. In a step towards understanding whether, or what type of, swarming behaviour of trypanosomes exists, we customised the assay for quantitative tracking analysis of motile behaviour on the single-cell level. We show that the migration speed of cell groups does not directly depend on single-cell velocity and that the system remains to be simplified further, before hypotheses about collective motility can be advanced.},
  language  = {en}
}
@article{KobsarKoehnlechnerKlingleretal.2022,
  author    = {Kobsar, Anna and Koehnlechner, Karina and Klingler, Philipp and Niklaus, Marius and Zeller-Hahn, Julia and Koessler, Angela and Weber, Katja and Boeck, Markus and Koessler, Juergen},
  title     = {The effect of short-term refrigeration on platelet responsiveness},
  series = {Scientific Reports},
  volume    = {12},
  journal   = {Scientific Reports},
  number    = {1},
  doi       = {10.1038/s41598-022-21124-4},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-301390},
  year      = {2022},
  abstract  = {Storage of platelet concentrates (PC) at cold temperature (CT) is discussed as an alternative to the current standard of storage at room temperature (RT). Recently, we could show that cold-induced attenuation of inhibitory signaling is an important mechanism promoting platelet reactivity. For developing strategies in blood banking, it is required to elucidate the time-dependent onset of facilitated platelet activation. Thus, freshly prepared platelet-rich-plasma (PRP) was stored for 1 and 2 h at CT (2-6 °C) or at RT (20-24 °C), followed by subsequent comparative analysis. Compared to RT, basal and induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation levels were decreased under CT within 1 h by approximately 20\%, determined by Western blot analysis and flow cytometry. Concomitantly, ADP- and collagen-induced threshold aggregation values were enhanced by up to 30-40\%. Furthermore, platelet-covered areas on collagen-coated slides and aggregate formation under flow conditions were increased after storage at CT, in addition to induced activation markers. In conclusion, a time period of 1-2 h for refrigeration is sufficient to induce an attenuation of inhibitory signaling, accompanied with an enhancement of platelet responsiveness. Short-term refrigeration may be considered as a rational approach to obtain PC with higher functional reactivity for the treatment of hemorrhage.},
  language  = {en}
}
@article{SchaeblerAmatobiHornetal.2020,
  author    = {Sch{\"a}bler, Stefan and Amatobi, Kelechi M. and Horn, Melanie and Rieger, Dirk and Helfrich‑F{\"o}rster, Charlotte and Mueller, Martin J. and Wegener, Christian and Fekete, Agnes},
  title     = {Loss of function in the Drosophila clock gene period results in altered intermediary lipid metabolism and increased susceptibility to starvation},
  series = {Cellular and Molecular Life Sciences},
  volume    = {77},
  journal   = {Cellular and Molecular Life Sciences},
  issn      = {1420-682X},
  doi       = {10.1007/s00018-019-03441-6},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232432},
  pages     = {4939-4956},
  year      = {2020},
  abstract  = {The fruit fly Drosophila is a prime model in circadian research, but still little is known about its circadian regulation of metabolism. Daily rhythmicity in levels of several metabolites has been found, but knowledge about hydrophobic metabolites is limited. We here compared metabolite levels including lipids between period\(^{01}\) (per\(^{01}\)) clock mutants and Canton-S wildtype (WT\(_{CS}\)) flies in an isogenic and non-isogenic background using LC-MS. In the non-isogenic background, metabo-lites with differing levels comprised essential amino acids, kynurenines, pterinates, glycero(phospho)lipids, and fatty acid esters. Notably, detectable diacylglycerols (DAG) and acylcarnitines (AC), involved in lipid metabolism, showed lower levels in per\(^{01}\) mutants. Most of these differences disappeared in the isogenic background, yet the level differences for AC as well as DAG were consistent for fly bodies. AC levels were dependent on the time of day in WTCS in phase with food consumption under LD conditions, while DAGs showed weak daily oscillations. Two short-chain ACs continued to cycle even in constant darkness. per\(^{01}\) mutants in LD showed no or very weak diel AC oscillations out of phase with feeding activity. The low levels of DAGs and ACs in per\(^{01}\) did not correlate with lower total food consumption, body mass or weight. Clock mutant flies showed higher sensitivity to starvation independent of their background-dependent activity level. Our results suggest that neither feeding, energy storage nor mobilisation is significantly affected in per\(^{01}\) mutants, but point towards impaired mitochondrial activity, supported by upregulation of the mitochondrial stress marker 4EBP in the clock mutants},
  language  = {en}
}
@article{VeepaschitViswanathanBordonneetal.2021,
  author    = {Veepaschit, Jyotishman and Viswanathan, Aravindan and Bordonne, Remy and Grimm, Clemens and Fischer, Utz},
  title     = {Identification and structural analysis of the Schizosaccharomyces pombe SMN complex},
  series = {Nucleic Acids Research},
  volume    = {49},
  journal   = {Nucleic Acids Research},
  number    = {13},
  doi       = {10.1093/nar/gkab158},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-259880},
  pages     = {7207-7223},
  year      = {2021},
  abstract  = {The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helices arranged with alternating polarity. This creates a central platform onto which Gemin8 binds and recruits Gemins 6 and 7. The N-terminal parts of the SMN molecules extrude via flexible linkers from the core and enable binding of Gemin2 and Sm proteins. Our data identify the SMN complex as a multivalent hub where Sm proteins are collected in its periphery to allow their joining with UsnRNA.},
  language  = {en}
}
@article{OberdoerferHeidrichBirnstieletal.2021,
  author    = {Oberd{\"o}rfer, Sebastian and Heidrich, David and Birnstiel, Sandra and Latoschik, Marc Erich},
  title     = {Enchanted by Your Surrounding? Measuring the Effects of Immersion and Design of Virtual Environments on Decision-Making},
  series = {Frontiers in Virtual Reality},
  volume    = {2},
  journal   = {Frontiers in Virtual Reality},
  doi       = {10.3389/frvir.2021.679277},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-260101},
  pages     = {679277},
  year      = {2021},
  abstract  = {Impaired decision-making leads to the inability to distinguish between advantageous and disadvantageous choices. The impairment of a person's decision-making is a common goal of gambling games. Given the recent trend of gambling using immersive Virtual Reality it is crucial to investigate the effects of both immersion and the virtual environment (VE) on decision-making. In a novel user study, we measured decision-making using three virtual versions of the Iowa Gambling Task (IGT). The versions differed with regard to the degree of immersion and design of the virtual environment. While emotions affect decision-making, we further measured the positive and negative affect of participants. A higher visual angle on a stimulus leads to an increased emotional response. Thus, we kept the visual angle on the Iowa Gambling Task the same between our conditions. Our results revealed no significant impact of immersion or the VE on the IGT. We further found no significant difference between the conditions with regard to positive and negative affect. This suggests that neither the medium used nor the design of the VE causes an impairment of decision-making. However, in combination with a recent study, we provide first evidence that a higher visual angle on the IGT leads to an effect of impairment.},
  language  = {en}
}
@article{PageWallstabeLotheretal.2021,
  author    = {Page, Lukas and Wallstabe, Julia and Lother, Jasmin and Bauser, Maximilian and Kniemeyer, Olaf and Strobel, Lea and Voltersen, Vera and Teutschbein, Janka and Hortschansky, Peter and Morton, Charles Oliver and Brakhage, Axel A. and Topp, Max and Einsele, Hermann and Wurster, Sebastian and Loeffler, Juergen},
  title     = {CcpA- and Shm2-Pulsed Myeloid Dendritic Cells Induce T-Cell Activation and Enhance the Neutrophilic Oxidative Burst Response to Aspergillus fumigatus},
  series = {Frontiers in Immunology},
  volume    = {12},
  journal   = {Frontiers in Immunology},
  issn      = {1664-3224},
  doi       = {10.3389/fimmu.2021.659752},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-239493},
  year      = {2021},
  abstract  = {Aspergillus fumigatus causes life-threatening opportunistic infections in immunocompromised patients. As therapeutic outcomes of invasive aspergillosis (IA) are often unsatisfactory, the development of targeted immunotherapy remains an important goal. Linking the innate and adaptive immune system, dendritic cells are pivotal in anti-Aspergillus defense and have generated interest as a potential immunotherapeutic approach in IA. While monocyte-derived dendritic cells (moDCs) require ex vivo differentiation, antigen-pulsed primary myeloid dendritic cells (mDCs) may present a more immediate platform for immunotherapy. To that end, we compared the response patterns and cellular interactions of human primary mDCs and moDCs pulsed with an A. fumigatus lysate and two A. fumigatus proteins (CcpA and Shm2) in a serum-free, GMP-compliant medium. CcpA and Shm2 triggered significant upregulation of maturation markers in mDCs and, to a lesser extent, moDCs. Furthermore, both A. fumigatus proteins elicited the release of an array of key pro-inflammatory cytokines including TNF-α, IL-1β, IL-6, IL-8, and CCL3 from both DC populations. Compared to moDCs, CcpA- and Shm2-pulsed mDCs exhibited greater expression of MHC class II antigens and stimulated stronger proliferation and IFN-γ secretion from autologous CD4\(^+\) and CD8\(^+\) T-cells. Moreover, supernatants of CcpA- and Shm2-pulsed mDCs significantly enhanced the oxidative burst in allogeneic neutrophils co-cultured with A. fumigatus germ tubes. Taken together, our in vitro data suggest that ex vivo CcpA- and Shm2-pulsed primary mDCs have the potential to be developed into an immunotherapeutic approach to tackle IA.},
  language  = {en}
}
@article{ClausHubertBecheretal.2019,
  author    = {Claus, Heike and Hubert, Kerstin and Becher, D{\"o}rte and Otto, Andreas and Pawlik, Marie-Christin and Lappann, Ines and Strobel, Lea and Vogel, Ulrich and Johswich, Kay},
  title     = {A homopolymeric adenosine tract in the promoter region of nspA influences factor H-mediated serum resistance in Neisseria meningitidis},
  series = {Scientific Reports},
  volume    = {9},
  journal   = {Scientific Reports},
  doi       = {10.1038/s41598-019-39231-0},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-200956},
  pages     = {2736},
  year      = {2019},
  abstract  = {Although usually asymptomatically colonizing the human nasopharynx, the Gram-negative bacterium Neisseria meningitidis (meningococcus) can spread to the blood stream and cause invasive disease. For survival in blood, N. meningitidis evades the complement system by expression of a polysaccharide capsule and surface proteins sequestering the complement regulator factor H (fH). Meningococcal strains belonging to the sequence type (ST-) 41/44 clonal complex (cc41/44) cause a major proportion of serogroup B meningococcal disease worldwide, but they are also common in asymptomatic carriers. Proteome analysis comparing cc41/44 isolates from invasive disease versus carriage revealed differential expression levels of the outer membrane protein NspA, which binds fH. Deletion of nspA reduced serum resistance and NspA expression correlated with fH sequestration. Expression levels of NspA depended on the length of a homopolymeric tract in the nspA promoter: A 5-adenosine tract dictated low NspA expression, whereas a 6-adenosine motif guided high NspA expression. Screening German cc41/44 strain collections revealed the 6-adenosine motif in 39\% of disease isolates, but only in 3.4\% of carriage isolates. Thus, high NspA expression is associated with disease, but not strictly required. The 6-adenosine nspA promoter is most common to the cc41/44, but is also found in other hypervirulent clonal complexes.},
  language  = {en}
}
@article{BankogluArnoldHeringetal.2018,
  author    = {Bankoglu, Ezgi Eyluel and Arnold, Charlotte and Hering, Ilona and Hankir, Mohammed and Seyfried, Florian and Stopper, Helga},
  title     = {Decreased chromosomal damage in lymphocytes of obese patients after bariatric surgery},
  series = {Scientific Reports},
  volume    = {8},
  journal   = {Scientific Reports},
  number    = {11195},
  doi       = {10.1038/s41598-018-29581-6},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-177090},
  year      = {2018},
  abstract  = {The number of bariatric surgeries being performed worldwide has markedly risen. While the improvement in obesity-associated comorbidities after bariatric surgery is well-established, very little is known about its impact on cancer risk. The peripheral lymphocyte micronucleus test is a widely used method for the monitoring of chromosomal damage levels in vivo, and micronucleus frequency positively correlates with cancer risk. Therefore, the aim of this study was to compare the micronucleus frequency before and after bariatric surgery in obese subjects. Peripheral blood mononuclear cells were collected from 45 obese subjects before and at two time-points after bariatric surgery (6 and 12 months) to assess spontaneous micronucleus frequency. Consistent with the increased cancer risk previously shown, bariatric surgery-induced weight loss led to a significant reduction in lymphocyte micronucleus frequency after 12 months. Interestingly, comorbidities such as type 2 diabetes mellitus and metabolic syndrome further seemed to have an impact on the lymphocyte micronucleus frequency. Our findings may indicate a successful reduction of cancer risk in patients following weight loss caused by bariatric surgery.},
  language  = {en}
}
@article{FriedrichHartigPruessetal.2022,
  author    = {Friedrich, Maximilian and Hartig, Johannes and Pr{\"u}ss, Harald and Ip, Wang Chi and Volkmann, Jens},
  title     = {Rapidly progressive dementia: Extending the spectrum of GFAP-astrocytopathies?},
  series = {Annals of Clinical and Translational Neurology},
  volume    = {9},
  journal   = {Annals of Clinical and Translational Neurology},
  number    = {3},
  doi       = {10.1002/acn3.51513},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-312957},
  pages     = {410-415},
  year      = {2022},
  abstract  = {Autoimmune glial fibrillary acidic protein astrocytopathy (GFAP-A) is a steroid-responsive meningoencephalomyelitis, sometimes presenting with atypical clinical signs such as movement disorders or psychiatric and autonomic features. Beyond clinical presentation and imaging, diagnosis relies on detection of GFAP-antibodies (AB) in CSF. Using quantitative behavioral, serologic, and immunohistochemical analyses, we characterize two patients longitudinally over 18-24 months who presented with rapidly progressive neurocognitive deterioration in the context of GFAP-AB in CSF and unremarkable cranial MRI studies. Intensified immunotherapy was associated with clinical stabilization. The value of GFAP-AB screening in selected cases of rapidly progressive dementias is discussed.},
  language  = {en}
}
@article{ManukjanWiegeringReindletal.2020,
  author    = {Manukjan, Georgi and Wiegering, Verena and Reindl, Tobias and Strauß, Gabriele and Klopocki, Eva and Schulze, Harald and Andres, Oliver},
  title     = {Novel variants in FERMT3 and RASGRP2 - Genetic linkage in Glanzmann-like bleeding disorders},
  series = {Pediatric Blood \& Cancer},
  volume    = {67},
  journal   = {Pediatric Blood \& Cancer},
  number    = {2},
  doi       = {10.1002/pbc.28078},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-208129},
  pages     = {e28078},
  year      = {2020},
  abstract  = {Defects of platelet intracellular signaling can result in severe platelet dysfunction. Several mutations in each of the linked genes FERMT3 and RASGRP2 on chromosome 11 causing a Glanzmann-like bleeding phenotype have been identified so far. We report on novel variants in two unrelated pediatric patients with severe bleeding diathesis—one with leukocyte adhesion deficiency type III due to a homozygous frameshift in FERMT3 and the other with homozygous variants in both, FERMT3 and RASGRP2 . We focus on the challenging genetic and functional variant assessment and aim to accentuate the risk of obtaining misleading results due to the phenomenon of genetic linkage.},
  language  = {en}
}
@article{KirchnerHerrmannMatrasetal.2022,
  author    = {Kirchner, Andr{\´e} and Herrmann, Nico and Matras, Paul and M{\"u}ller, Iris and Meister, Julia and Schattner, Thomas G.},
  title     = {A pedo-geomorphological view on land use and its potential in the surroundings of the ancient Hispano-Roman city Munigua (Seville, SW Spain)},
  series = {E\&G Quaternary Science Journal},
  volume    = {71},
  journal   = {E\&G Quaternary Science Journal},
  number    = {2},
  doi       = {10.5194/egqsj-71-123-2022},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-300141},
  pages     = {123 -- 143},
  year      = {2022},
  abstract  = {This study investigates the surroundings of Munigua (municipium Flavium Muniguense), a small Roman town in the ancient province of Hispania Baetica (SW Spain). The city's economy was based primarily on copper and iron mining, which brought financial prosperity to its citizens. Local production of agricultural goods is thought to have been of little importance, as the regional soil conditions do not seem to be suitable for extensive agriculture. To evaluate the recent soil agro-potential and to find evidence for prehistoric and historic land use in the surroundings of Munigua, we applied a pedo-geomorphological approach based on the physico-chemical analysis of 14 representative soil and sediment exposures. Selected samples were analyzed for bulk chemistry, texture and phytoliths. The chronostratigraphy of the sequences was based on radiocarbon dating of charcoal samples. The site evaluation of the present-day soil agro-potential was carried out according to standard procedures and included evaluation of potential rootability, available water-storage capacity and nutrient budget within the uppermost 1 m. The results show that moderate to very good soil agro-potential prevails in the granitic and floodplain areas surrounding Munigua. Clearly, recent soil agro-potential in these areas allows the production of basic agricultural goods, and similar limited agricultural use should also have been possible in ancient times. In contrast, weak to very weak present-day soil agro-potential prevails in the metamorphic landscape due to the occurrence of shallow and sandy to stony soils. In addition, the study provides pedo-geomorphological evidence for prehistoric and historic land use in pre-Roman, Roman and post-Roman times. Catenary soil mapping in the vicinity of a Roman house complex reveals multi-layered colluvial deposits. They document phases of hillslope erosion mainly triggered by human land use between 4063 ± 82 and 3796 ± 76 cal BP, around 2601 ± 115 cal BP, and between 1424 ± 96 and 421 ± 88 cal BP. Moreover, geochemical and phytolith analyses of a Roman hortic Anthrosol indicate the local cultivation of agricultural products that contributed to the food supply of Munigua. Overall, the evidence of Roman agricultural use in the Munigua area indicates that the city's economy was by no means focused solely on mining. The production of basic agricultural products was also part of Munigua's economic portfolio. Our geoarcheological study thus supports the archeological concept of economically diversified Roman cities in the province of Baetica and in Hispania.},
  language  = {en}
}
@article{TinajeroTrejoRanaNageletal.2016,
  author    = {Tinajero-Trejo, Mariana and Rana, Namrata and Nagel, Christoph and Jesse, Helen E. and Smith, Thomas W. and Wareham, Lauren K. and Hippler, Michael and Schatzschneider, Ulrich and Poole, Robert K.},
  title     = {Antimicrobial Activity of the Manganese Photoactivated Carbon Monoxide-Releasing Molecule [Mn(CO)\(_3\)(tpa-kappa\(^3\)N)]\(^+\) Against a Pathogenic Escherichia coli that Causes Urinary Infections},
  series = {Antioxidants \& Redox Signaling},
  volume    = {24},
  journal   = {Antioxidants \& Redox Signaling},
  number    = {14},
  doi       = {10.1089/ars.2015.6484},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-188910},
  pages     = {765-780},
  year      = {2016},
  abstract  = {Aims: We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)\(_3\)(tpa-kappa\(^3\)N)]\(^+\)) against an antibiotic-resistant uropathogenic strain (EC958) of Escherichia coli. Results: Activated PhotoCORM inhibits growth and decreases viability of E. coli EC958, but non-illuminated carbon monoxide-releasing molecule (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking the effect of dissolved CO gas. CO from the PhotoCORM binds to intracellular targets, namely respiratory oxidases in strain EC958 and a bacterial globin heterologously expressed in strain K-12. However, unlike previously characterized CORMs, the PhotoCORM is not significantly accumulated in cells, as deduced from the cellular manganese content. Activated PhotoCORM reacts avidly with hydrogen peroxide producing hydroxyl radicals; the observed peroxide-enhanced toxicity of the PhotoCORM is ameliorated by thiourea. The PhotoCORM also potentiates the effect of the antibiotic, doxycycline. Innovation: The present work investigates for the first time the antimicrobial activity of a light-activated PhotoCORM against an antibiotic-resistant pathogen. A comprehensive study of the effects of the PhotoCORM and its derivative molecules upon illumination is performed and mechanisms of toxicity of the activated PhotoCORM are investigated. Conclusion: The PhotoCORM allows a site-specific and time-controlled release of CO in bacterial cultures and has the potential to provide much needed information on the generality of CORM activities in biology. Understanding the mechanism(s) of activated PhotoCORM toxicity will be key in exploring the potential of this and similar compounds as antimicrobial agents, perhaps in combinatorial therapies with other agents.},
  language  = {en}
}
@article{TanBabakVenkatesanetal.2019,
  author    = {Tan, Aaron and Babak, Maria V. and Venkatesan, Gopalakrishnan and Lim, Clarissa and Klotz, Karl-Norbert and Herr, Deron Raymond and Cheong, Siew Lee and Federico, Stephanie and Spalluto, Giampiero and Ong, Wei-Yi and Chen, Yu Zong and Loo, Jason Siau Ee and Pastorin, Giorgia},
  title     = {Design, Synthesis and Evaluation of New Indolylpyrimidylpiperazines for Gastrointestinal Cancer Therapy},
  series = {Molecules},
  volume    = {24},
  journal   = {Molecules},
  number    = {20},
  issn      = {1420-3049},
  doi       = {10.3390/molecules24203661},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-193271},
  pages     = {3661},
  year      = {2019},
  abstract  = {Human A3 adenosine receptor hA3AR has been implicated in gastrointestinal cancer, where its cellular expression has been found increased, thus suggesting its potential as a molecular target for novel anticancer compounds. Observation made in our previous work indicated the importance of the carbonyl group of amide in the indolylpyrimidylpiperazine (IPP) for its human A2A adenosine receptor (hA2AAR) subtype binding selectivity over the other AR subtypes. Taking this observation into account, we structurally modified an indolylpyrimidylpiperazine (IPP) scaffold, 1 (a non-selective adenosine receptors' ligand) into a modified IPP (mIPP) scaffold by switching the position of the carbonyl group, resulting in the formation of both ketone and tertiary amine groups in the new scaffold. Results showed that such modification diminished the A2A activity and instead conferred hA3AR agonistic activity. Among the new mIPP derivatives (3-6), compound 4 showed potential as a hA3AR partial agonist, with an Emax of 30\% and EC50 of 2.89 ± 0.55 μM. In the cytotoxicity assays, compound 4 also exhibited higher cytotoxicity against both colorectal and liver cancer cells as compared to normal cells. Overall, this new series of compounds provide a promising starting point for further development of potent and selective hA3AR partial agonists for the treatment of gastrointestinal cancers.},
  language  = {en}
}
@article{SperlichDeClerckZinneretal.2018,
  author    = {Sperlich, Billy and De Clerck, Ine and Zinner, Christoph and Holmberg,, Hans-Christer and Wallmann-Sperlich, Birgit},
  title     = {Prolonged sitting interrupted by 6-min of high-intensity exercise: circulatory, metabolic, hormonal, thermal, cognitive, and perceptual responses},
  series = {Frontiers in Physiology},
  volume    = {9},
  journal   = {Frontiers in Physiology},
  number    = {1279},
  doi       = {10.3389/fphys.2018.01279},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-177307},
  year      = {2018},
  abstract  = {The aim was to examine certain aspects of circulatory, metabolic, hormonal, thermoregulatory, cognitive, and perceptual responses while sitting following a brief session of high-intensity interval exercise. Twelve students (five men; age, 22 ± 2 years) performed two trials involving either simply sitting for 180 min (SIT) or sitting for this same period with a 6-min session of high-intensity exercise after 60 min (SIT+HIIT). At T\(_0\) (after 30 min of resting), T\(_1\) (after a 20-min breakfast), T\(_2\) (after sitting for 1 h), T\(_3\) (immediately after the HIIT), T\(_4\), T\(_5\), T\(_6\), and T\(_7\) (30, 60, 90, and 120 min after the HIIT), circulatory, metabolic, hormonal, thermoregulatory, cognitive, and perceptual responses were assessed. The blood lactate concentration (at T\(_3\)-T\(_5\)), heart rate (at T\(_3\)-T\(_6\)), oxygen uptake (at T\(_3\)-T\(_7\)), respiratory exchange ratio, and sensations of heat (T\(_3\)-T\(_5\)), sweating (T\(_3\), T\(_4\)) and odor (T\(_3\)), as well as perception of vigor (T\(_3\)-T\(_6\)), were higher and the respiratory exchange ratio (T\(_4\)-T\(_7\)) and mean body and skin temperatures (T\(_3\)) lower in the SIT+HIIT than the SIT trial. Levels of blood glucose and salivary cortisol, cerebral oxygenation, and feelings of anxiety/depression, fatigue or hostility, as well as the variables of cognitive function assessed by the Stroop test did not differ between SIT and SIT+HIIT. In conclusion, interruption of prolonged sitting with a 6-min session of HIIT induced more pronounced circulatory and metabolic responses and improved certain aspects of perception, without affecting selected hormonal, thermoregulatory or cognitive functions.},
  language  = {en}
}
@article{KressJessenMarquardtetal.2021,
  author    = {Kreß, Julia Katharina Charlotte and Jessen, Christina and Marquardt, Andr{\´e} and Hufnagel, Anita and Meierjohann, Svenja},
  title     = {NRF2 enables EGFR signaling in melanoma cells},
  series = {International Journal of Molecular Sciences},
  volume    = {22},
  journal   = {International Journal of Molecular Sciences},
  number    = {8},
  issn      = {1422-0067},
  doi       = {10.3390/ijms22083803},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-260222},
  year      = {2021},
  abstract  = {Receptor tyrosine kinases (RTK) are rarely mutated in cutaneous melanoma, but the expression and activation of several RTK family members are associated with a proinvasive phenotype and therapy resistance. Epidermal growth factor receptor (EGFR) is a member of the RTK family and is only expressed in a subgroup of melanomas with poor prognosis. The insight into regulators of EGFR expression and activation is important for the understanding of the development of this malignant melanoma phenotype. Here, we describe that the transcription factor NRF2, the master regulator of the oxidative and electrophilic stress response, mediates the expression and activation of EGFR in melanoma by elevating the levels of EGFR as well as its ligands EGF and TGFα. ChIP sequencing data show that NRF2 directly binds to the promoter of EGF, which contains a canonical antioxidant response element. Accordingly, EGF is induced by oxidative stress and is also increased in lung adenocarcinoma and head and neck carcinoma with mutationally activated NRF2. In contrast, regulation of EGFR and TGFA occurs by an indirect mechanism, which is enabled by the ability of NRF2 to block the activity of the melanocytic lineage factor MITF in melanoma. MITF effectively suppresses EGFR and TGFA expression and therefore serves as link between NRF2 and EGFR. As EGFR was previously described to stimulate NRF2 activity, the mutual activation of NRF2 and EGFR pathways was investigated. The presence of NRF2 was necessary for full EGFR pathway activation, as NRF2-knockout cells showed reduced AKT activation in response to EGF stimulation compared to controls. Conversely, EGF led to the nuclear localization and activation of NRF2, thereby demonstrating that NRF2 and EGFR are connected in a positive feedback loop in melanoma. In summary, our data show that the EGFR-positive melanoma phenotype is strongly supported by NRF2, thus revealing a novel maintenance mechanism for this clinically challenging melanoma subpopulation.},
  language  = {en}
}
@article{BraunEvdokimovFranketal.2022,
  author    = {Braun, Alexandra and Evdokimov, Dimitar and Frank, Johanna and Pauli, Paul and Wabel, Thomas and {\"U}{\c{c}}eyler, Nurcan and Sommer, Claudia},
  title     = {Relevance of Religiosity for Coping Strategies and Disability in Patients with Fibromyalgia Syndrome},
  series = {Journal of Religion and Health},
  volume    = {61},
  journal   = {Journal of Religion and Health},
  number    = {1},
  issn      = {1573-6571},
  doi       = {10.1007/s10943-020-01177-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-269135},
  pages     = {524-539},
  year      = {2022},
  abstract  = {Coping strategies are essential for the outcome of chronic pain. This study evaluated religiosity in a cohort of patients with fibromyalgia syndrome (FMS), its effect on pain and other symptoms, on coping and FMS-related disability. A total of 102 FMS patients were recruited who filled in questionnaires, a subgroup of 42 patients participated in a face-to-face interview, and data were evaluated by correlation and regression analyses. Few patients were traditionally religious, but the majority believed in a higher existence and described their spirituality as "transcendence conviction". The coping strategy "praying-hoping" and the ASP dimension "religious orientation" (r = 0.5, P < 0.05) showed a significant relationship independent of the grade of religiosity (P < 0.05). A high grade of belief in a higher existence was negatively associated with the choice of ignoring as coping strategy (r = - 0.4, P < 0.05). Mood and affect-related variables had the highest impact on disability (b = 0.5, P < 0.05). In this cohort, the grade of religiosity played a role in the choice of coping strategies, but had no effects on health and mood outcome.},
  language  = {en}
}
@article{StreinzerChakravortyNeumayeretal.2019,
  author    = {Streinzer, Martin and Chakravorty, Jharna and Neumayer, Johann and Megu, Karsing and Narah, Jaya and Schmitt, Thomas and Bharti, Himender and Spaethe, Johannes and Brockmann, Axel},
  title     = {Species composition and elevational distribution of bumble bees (Hymenoptera, Apidae, Bombus Latreille) in the East Himalaya, Arunachal Pradesh, India},
  series = {ZooKeys},
  volume    = {851},
  journal   = {ZooKeys},
  doi       = {10.3897/zookeys.851.32956},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-201937},
  pages     = {71-89},
  year      = {2019},
  abstract  = {The East Himalaya is one of the world's most biodiverse ecosystems. However, very little is known about the abundance and distribution of many plant and animal taxa in this region. Bumble bees are a group of cold-adapted and high elevation insects that fulfil an important ecological and economical function as pollinators of wild and agricultural flowering plants and crops. The Himalayan mountain range provides ample suitable habitats for bumble bees. Systematic study of Himalayan bumble bees began a few decades ago and the main focus has centred on the western region, while the eastern part of the mountain range has received little attention and only a few species have been verified. During a three-year survey, more than 700 bumble bee specimens of 21 species were collected in Arunachal Pradesh, the largest of the north-eastern states of India. The material included a range of species that were previously known from a limited number of collected specimens, which highlights the unique character of the East Himalayan ecosystem. Our results are an important first step towards a future assessment of species distribution, threat, and conservation. Clear elevation patterns of species diversity were observed, which raise important questions about the functional adaptations that allow bumble bees to thrive in this particularly moist region in the East Himalaya.},
  language  = {en}
}
@article{LangeAthinodorou2021,
  author    = {Lange-Athinodorou, Eva},
  title     = {Implications of geoarchaeological investigations for the contextualization of sacred landscapes in the Nile Delta},
  series = {E\&G Quarternary Science Journal},
  volume    = {70},
  journal   = {E\&G Quarternary Science Journal},
  number    = {1},
  doi       = {10.5194/egqsj-70-73-2021},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-258688},
  pages     = {73-82},
  year      = {2021},
  abstract  = {Key elements of sacred landscapes of the Nile Delta were lakes, canals and artificial basins connected to temples, which were built on elevated terrain. In the case of temples of goddesses of an ambivalent, even dangerous, nature, i.e. lioness goddesses and all female deities who could appear as such, the purpose of sacred lakes and canals exceeded their function as a water resource for basic practical and religious needs. Their pleasing coolness was believed to calm the goddess' fiery nature, and during important religious festivals, the barques of the goddesses were rowed on those waters. As archaeological evidence was very rare in the past, the study of those sacred waters was mainly confined to textual sources. Recently applied geoarchaeological methods, however, have changed this situation dramatically: they allow in-depth investigations and reconstructions of these deltaic sacred landscapes. Exploring these newly available data, the paper presented here focuses on the sites of Buto, Sais and Bubastis, by investigating the characteristics of their sacred lakes, canals and marshes with respect to their hydrogeographical and geomorphological context and to their role in ancient Egyptian religion and mythology as well.},
  language  = {en}
}
@article{HankeRauschSosnowskietal.2022,
  author    = {Hanke, Kathrin and Rausch, Tanja K. and Sosnowski, Runa and Paul, Pia and Spiegler, Juliane and M{\"u}ller, Mirja and K{\"o}nig, Inke R. and G{\"o}pel, Wolfgang and Herting, Egbert and H{\"a}rtel, Christoph},
  title     = {Early skin-to-skin contact does not affect cerebral tissue oxygenation in preterm infants <32 weeks of gestation},
  series = {Children},
  volume    = {9},
  journal   = {Children},
  number    = {2},
  issn      = {2227-9067},
  doi       = {10.3390/children9020211},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-262290},
  year      = {2022},
  abstract  = {Aim: It was the aim of our study to determine the regional cerebral tissue oxygenation saturation (rcSO\(_2\)) as an additional monitoring parameter during early skin-to-skin contact (SSC) in preterm infants with a gestational age of <32 gestational weeks. Methods: We conducted two observational convenience sample studies using additional monitoring with near-infrared spectroscopy (NIRS) in the first 120 h of life: (a) NIRS 1 (gestational age of 26 0/7 to 31 6/7 weeks) and (b) NIRS 2 (gestational age of 24 0/7 to 28 6/7 weeks). The rcSO\(_2\) values were compared between resting time in the incubator (period I), SSC (period II) and handling nursing care (period III). For the comparison, we separated the sequential effects by including a "wash-out phase" of 1 h between each period. Results: During the first 120 h of life 38/53 infants in NIRS 1 and 15/23 infants in NIRS 2 received SSC, respectively. We found no remarkable differences for rcSO\(_2\) values of NIRS 1 patients between SSC time and period I (95\% confidence interval (CI) for the difference in \%: SSC vs. period I [1; 3]). In NIRS 2, rcSO\(_2\) values during SSC were only 2\% lower compared with period I [median [1. quartile; 3. quartile] in \%; 78 [73; 82] vs. 80 [74; 85]] but were similar to period III [78 [72; 83]]. In a combined analysis, a small difference in rcSO\(_2\) values between SSC and resting times was found using a generalized linear mixed model that included gender and gestational age (OR 95\% CI; 1.178 [1.103; 1.253], p < 0.0001). Episodes below the cut-off for "hypoxia"; e.g., <55\%, were comparable during SSC and periods I and III (0.3-2.1\%). No FiO\(_2\) adjustment was required in the vast majority of SSC episodes. Conclusions: Our observational data indicate that rcSO\(_2\) values of infants during SSC were comparable to rcSO\(_2\) values during incubator care and resting time. This additional monitoring supports a safe implementation of early SSC in extremely preterm infants in NICUs.},
  language  = {en}
}
@article{SteudingSuriajaya2020,
  author    = {Steuding, J{\"o}rn and Suriajaya, Ade Irma},
  title     = {Value-Distribution of the Riemann Zeta-Function Along Its Julia Lines},
  series = {Computational Methods and Function Theory},
  volume    = {20},
  journal   = {Computational Methods and Function Theory},
  issn      = {1617-9447},
  doi       = {10.1007/s40315-020-00316-x},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232621},
  pages     = {389-401},
  year      = {2020},
  abstract  = {For an arbitrary complex number a≠0 we consider the distribution of values of the Riemann zeta-function ζ at the a-points of the function Δ which appears in the functional equation ζ(s)=Δ(s)ζ(1-s). These a-points δa are clustered around the critical line 1/2+i\(\mathbb {R}\) which happens to be a Julia line for the essential singularity of ζ at infinity. We observe a remarkable average behaviour for the sequence of values ζ(δ\(_a\)).},
  language  = {en}
}
@article{StelznerBoynyHertleinetal.2021,
  author    = {Stelzner, Kathrin and Boyny, Aziza and Hertlein, Tobias and Sroka, Aneta and Moldovan, Adriana and Paprotka, Kerstin and Kessie, David and Mehling, Helene and Potempa, Jan and Ohlsen, Knut and Fraunholz, Martin J. and Rudel, Thomas},
  title     = {Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells},
  series = {PLoS Pathogens},
  volume    = {17},
  journal   = {PLoS Pathogens},
  number    = {9},
  doi       = {10.1371/journal.ppat.1009874},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-263908},
  year      = {2021},
  abstract  = {Staphylococcus aureus is a major human pathogen, which can invade and survive in non-professional and professional phagocytes. Uptake by host cells is thought to contribute to pathogenicity and persistence of the bacterium. Upon internalization by epithelial cells, cytotoxic S. aureus strains can escape from the phagosome, replicate in the cytosol and induce host cell death. Here, we identified a staphylococcal cysteine protease to induce cell death after translocation of intracellular S. aureus into the host cell cytoplasm. We demonstrated that loss of staphopain A function leads to delayed onset of host cell death and prolonged intracellular replication of S. aureus in epithelial cells. Overexpression of staphopain A in a non-cytotoxic strain facilitated intracellular killing of the host cell even in the absence of detectable intracellular replication. Moreover, staphopain A contributed to efficient colonization of the lung in a mouse pneumonia model. In phagocytic cells, where intracellular S. aureus is exclusively localized in the phagosome, staphopain A did not contribute to cytotoxicity. Our study suggests that staphopain A is utilized by S. aureus to exit the epithelial host cell and thus contributes to tissue destruction and dissemination of infection. Author summary Staphylococcus aureus is an antibiotic-resistant pathogen that emerges in hospital and community settings and can cause a variety of diseases ranging from skin abscesses to lung inflammation and blood poisoning. The bacterium can asymptomatically colonize the upper respiratory tract and skin of humans and take advantage of opportune conditions, like immunodeficiency or breached barriers, to cause infection. Although S. aureus was not regarded as intracellular bacterium, it can be internalized by human cells and subsequently exit the host cells by induction of cell death, which is considered to cause tissue destruction and spread of infection. The bacterial virulence factors and underlying molecular mechanisms involved in the intracellular lifestyle of S. aureus remain largely unknown. We identified a bacterial cysteine protease to contribute to host cell death of epithelial cells mediated by intracellular S. aureus. Staphopain A induced killing of the host cell after translocation of the pathogen into the cell cytosol, while bacterial proliferation was not required. Further, the protease enhanced survival of the pathogen during lung infection. These findings reveal a novel, intracellular role for the bacterial protease staphopain A.},
  language  = {en}
}
@article{GerovaWickeChiharaetal.2021,
  author    = {Gerova, Milan and Wicke, Laura and Chihara, Kotaro and Schneider, Cornelius and Lavigne, Rob and Vogel, J{\"o}rg},
  title     = {A grad-seq view of RNA and protein complexes in Pseudomonas aeruginosa under standard and bacteriophage predation conditions},
  series = {mbio},
  volume    = {12},
  journal   = {mbio},
  number    = {1},
  doi       = {10.1128/mBio.03454-20},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-259054},
  pages     = {e03454-20},
  year      = {2021},
  abstract  = {The Gram-negative rod-shaped bacterium Pseudomonas aeruginosa is not only a major cause of nosocomial infections but also serves as a model species of bacterial RNA biology. While its transcriptome architecture and posttranscriptional regulation through the RNA-binding proteins Hfq, RsmA, and RsmN have been studied in detail, global information about stable RNA-protein complexes in this human pathogen is currently lacking. Here, we implement gradient profiling by sequencing (Grad-seq) in exponentially growing P. aeruginosa cells to comprehensively predict RNA and protein complexes, based on glycerol gradient sedimentation profiles of >73\% of all transcripts and ∼40\% of all proteins. As to benchmarking, our global profiles readily reported complexes of stable RNAs of P. aeruginosa, including 6S RNA with RNA polymerase and associated product RNAs (pRNAs). We observe specific clusters of noncoding RNAs, which correlate with Hfq and RsmA/N, and provide a first hint that P. aeruginosa expresses a ProQ-like FinO domain-containing RNA-binding protein. To understand how biological stress may perturb cellular RNA/protein complexes, we performed Grad-seq after infection by the bacteriophage ΦKZ. This model phage, which has a well-defined transcription profile during host takeover, displayed efficient translational utilization of phage mRNAs and tRNAs, as evident from their increased cosedimentation with ribosomal subunits. Additionally, Grad-seq experimentally determines previously overlooked phage-encoded noncoding RNAs. Taken together, the Pseudomonas protein and RNA complex data provided here will pave the way to a better understanding of RNA-protein interactions during viral predation of the bacterial cell. IMPORTANCE Stable complexes by cellular proteins and RNA molecules lie at the heart of gene regulation and physiology in any bacterium of interest. It is therefore crucial to globally determine these complexes in order to identify and characterize new molecular players and regulation mechanisms. Pseudomonads harbor some of the largest genomes known in bacteria, encoding ∼5,500 different proteins. Here, we provide a first glimpse on which proteins and cellular transcripts form stable complexes in the human pathogen Pseudomonas aeruginosa. We additionally performed this analysis with bacteria subjected to the important and frequently encountered biological stress of a bacteriophage infection. We identified several molecules with established roles in a variety of cellular pathways, which were affected by the phage and can now be explored for their role during phage infection. Most importantly, we observed strong colocalization of phage transcripts and host ribosomes, indicating the existence of specialized translation mechanisms during phage infection. All data are publicly available in an interactive and easy to use browser.},
  language  = {en}
}
@article{DoryabTaskinStahlhutetal.2021,
  author    = {Doryab, Ali and Taskin, Mehmet Berat and Stahlhut, Philipp and Schr{\"o}ppel, Andreas and Wagner, Darcy E. and Groll, J{\"u}rgen and Schmid, Otmar},
  title     = {A Biomimetic, Copolymeric Membrane for Cell-Stretch Experiments with Pulmonary Epithelial Cells at the Air-Liquid Interface},
  series = {Advanced Functional Materials},
  volume    = {31},
  journal   = {Advanced Functional Materials},
  number    = {10},
  doi       = {10.1002/adfm.202004707},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-225645},
  year      = {2021},
  abstract  = {Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (≤5 μm) is stretchable (up to 25\% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air-liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.},
  language  = {en}
}
@article{GergsJahnSchulzetal.2022,
  author    = {Gergs, Ulrich and Jahn, Tina and Schulz, Nico and Großmann, Claudia and Rueckschloss, Uwe and Demus, Uta and Buchwalow, Igor B. and Neumann, Joachim},
  title     = {Protein phosphatase 2A improves cardiac functional response to ischemia and sepsis},
  series = {International Journal of Molecular Sciences},
  volume    = {23},
  journal   = {International Journal of Molecular Sciences},
  number    = {9},
  issn      = {1422-0067},
  doi       = {10.3390/ijms23094688},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-284035},
  year      = {2022},
  abstract  = {Reversible protein phosphorylation is a posttranslational modification of regulatory proteins involved in cardiac signaling pathways. Here, we focus on the role of protein phosphatase 2A (PP2A) for cardiac gene expression and stress response using a transgenic mouse model with cardiac myocyte-specific overexpression of the catalytic subunit of PP2A (PP2A-TG). Gene and protein expression were assessed under basal conditions by gene chip analysis and Western blotting. Some cardiac genes related to the cell metabolism and to protein phosphorylation such as kinases and phosphatases were altered in PP2A-TG compared to wild type mice (WT). As cardiac stressors, a lipopolysaccharide (LPS)-induced sepsis in vivo and a global cardiac ischemia in vitro (stop-flow isolated perfused heart model) were examined. Whereas the basal cardiac function was reduced in PP2A-TG as studied by echocardiography or as studied in the isolated work-performing heart, the acute LPS- or ischemia-induced cardiac dysfunction deteriorated less in PP2A-TG compared to WT. From the data, we conclude that increased PP2A activity may influence the acute stress tolerance of cardiac myocytes.},
  language  = {en}
}
@article{HoltfrerichSchwarzSprengeretal.2016,
  author    = {Holtfrerich, Sarah K. C. and Schwarz, Katharina A. and Sprenger, Christian and Reimers, Luise and Diekhof, Esther K.},
  title     = {Endogenous Testosterone and Exogenous Oxytocin Modulate Attentional Processing of Infant Faces},
  series = {PLoS ONE},
  volume    = {11},
  journal   = {PLoS ONE},
  number    = {11},
  doi       = {10.1371/journal.pone.0166617},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-166783},
  pages     = {e0166617},
  year      = {2016},
  abstract  = {Evidence indicates that hormones modulate the intensity of maternal care. Oxytocin is known for its positive influence on maternal behavior and its important role for childbirth. In contrast, testosterone promotes egocentric choices and reduces empathy. Further, testosterone decreases during parenthood which could be an adaptation to increased parental investment. The present study investigated the interaction between testosterone and oxytocin in attentional control and their influence on attention to baby schema in women. Higher endogenous testosterone was expected to decrease selective attention to child portraits in a face-in-the-crowd-paradigm, while oxytocin was expected to counteract this effect. As predicted, women with higher salivary testosterone were slower in orienting attention to infant targets in the context of adult distractors. Interestingly, reaction times to infant and adult stimuli decreased after oxytocin administration, but only in women with high endogenous testosterone. These results suggest that oxytocin may counteract the adverse effects of testosterone on a central aspect of social behavior and maternal caretaking.},
  language  = {en}
}
@article{RymaTylekLiebscheretal.2021,
  author    = {Ryma, Matthias and Tylek, Tina and Liebscher, Julia and Blum, Carina and Fernandez, Robin and B{\"o}hm, Christoph and Kastenm{\"u}ller, Wolfgang and Gasteiger, Georg and Groll, J{\"u}rgen},
  title     = {Translation of collagen ultrastructure to biomaterial fabrication for material-independent but highly efficient topographic immunomodulation},
  series = {Advanced materials},
  volume    = {33},
  journal   = {Advanced materials},
  number    = {33},
  doi       = {10.1002/adma.202101228},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-256381},
  year      = {2021},
  abstract  = {Supplement-free induction of cellular differentiation and polarization solely through the topography of materials is an auspicious strategy but has so far significantly lagged behind the efficiency and intensity of media-supplementation-based protocols. Consistent with the idea that 3D structural motifs in the extracellular matrix possess immunomodulatory capacity as part of the natural healing process, it is found in this study that human-monocyte-derived macrophages show a strong M2a-like prohealing polarization when cultured on type I rat-tail collagen fibers but not on collagen I films. Therefore, it is hypothesized that highly aligned nanofibrils also of synthetic polymers, if packed into larger bundles in 3D topographical biomimetic similarity to native collagen I, would induce a localized macrophage polarization. For the automated fabrication of such bundles in a 3D printing manner, the strategy of "melt electrofibrillation" is pioneered by the integration of flow-directed polymer phase separation into melt electrowriting and subsequent selective dissolution of the matrix polymer postprocessing. This process yields nanofiber bundles with a remarkable structural similarity to native collagen I fibers, particularly for medical-grade poly(ε-caprolactone). These biomimetic fibrillar structures indeed induce a pronounced elongation of human-monocyte-derived macrophages and unprecedentedly trigger their M2-like polarization similar in efficacy as interleukin-4 treatment.},
  language  = {en}
}
@article{BischoffRieferWirthensohnetal.2020,
  author    = {Bischoff, Lisa A. and Riefer, Jarno and Wirthensohn, Raphael and Bischof, Tobias and Bertermann, R{\"u}diger and Ignat'ev, Nikolai V. and Finze, Maik},
  title     = {Pentafluoroethylaluminates: A Combined Synthetic, Spectroscopic, and Structural Study},
  series = {Chemistry - A European Journal},
  volume    = {26},
  journal   = {Chemistry - A European Journal},
  number    = {60},
  doi       = {10.1002/chem.202000667},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-214666},
  pages     = {13615 -- 13620},
  year      = {2020},
  abstract  = {Salts of the tetrakis(pentafluoroethyl)aluminate anion [Al(C\(_{2}\)F\(_{5}\))\(_{2}\)]\(^{-}\) were obtained from AlCl\(_{3}\) and LiC\(_{2}\)F\(_{5}\). They were isolated with different counter-cations and characterized by NMR and vibrational spectroscopy and mass spectrometry. Degradation of the [Al(C\(_{2}\)F\(_{5}\))\(_{4}\)]\(^{-}\) ion was found to proceed via 1,2-fluorine shifts and stepwise loss of CF(CF\(_{3}\)) under formation of [(C\(_{2}\)F\(_{5}\))\(_{4-n}\)AlF\(_{n}\)]- (n=1-4) as assessed by NMR spectroscopy and mass spectrometry and supported by results of DFT calculations. In addition, the [(C\(_{2}\)F\(_{5}\))AlF\(_{3}\)]\(^{-}\) ion was structurally characterized.},
  language  = {en}
}
@article{KirschKitzmannKunde2021,
  author    = {Kirsch, Wladimir and Kitzmann, Tim and Kunde, Wilfried},
  title     = {Action affects perception through modulation of attention},
  series = {Attention, Perception \& Psychophysics},
  volume    = {83},
  journal   = {Attention, Perception \& Psychophysics},
  number    = {5},
  issn      = {1943-393X},
  doi       = {10.3758/s13414-021-02277-2},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-273176},
  pages     = {2320-2330},
  year      = {2021},
  abstract  = {The present study explored the origin of perceptual changes repeatedly observed in the context of actions. In Experiment 1, participants tried to hit a circular target with a stylus movement under restricted feedback conditions. We measured the perception of target size during action planning and observed larger estimates for larger movement distances. In Experiment 2, we then tested the hypothesis that this action specific influence on perception is due to changes in the allocation of spatial attention. For this purpose, we replaced the hitting task by conditions of focused and distributed attention and measured the perception of the former target stimulus. The results revealed changes in the perceived stimulus size very similar to those observed in Experiment 1. These results indicate that action's effects on perception root in changes of spatial attention.},
  language  = {en}
}
@unpublished{BrychHaendel2020,
  author    = {Brych, Mareike and H{\"a}ndel, Barbara},
  title     = {Disentangling top-down and bottom-up influences on blinks in the visual and auditory domain},
  series = {International Journal of Psychophysiology},
  journal   = {International Journal of Psychophysiology},
  issn      = {1872-7697},
  doi       = {10.1016/j.ijpsycho.2020.11.002},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-246590},
  year      = {2020},
  abstract  = {Sensory input as well as cognitive factors can drive the modulation of blinking. Our aim was to dissociate sensory driven bottom-up from cognitive top-down influences on blinking behavior and compare these influences between the auditory and the visual domain. Using an oddball paradigm, we found a significant pre-stimulus decrease in blink probability for visual input compared to auditory input. Sensory input further led to an early post-stimulus blink increase in both modalities if a task demanded attention to the input. Only visual input caused a pronounced early increase without a task. In case of a target or the omission of a stimulus (as compared to standard input), an additional late increase in blink rate was found in the auditory and visual domain. This suggests that blink modulation must be based on the interpretation of the input, but does not need any sensory input at all to occur. Our results show a complex modulation of blinking based on top-down factors such as prediction and attention in addition to sensory-based influences. The magnitude of the modulation is mainly influenced by general attentional demands, while the latency of this modulation allows to dissociate general from specific top-down influences that are independent of the sensory domain.},
  language  = {en}
}
@article{WasmusDudek2020,
  author    = {Wasmus, Christina and Dudek, Jan},
  title     = {Metabolic Alterations Caused by Defective Cardiolipin Remodeling in Inherited Cardiomyopathies},
  series = {Life},
  volume    = {10},
  journal   = {Life},
  number    = {11},
  issn      = {2075-1729},
  doi       = {10.3390/life10110277},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-219286},
  year      = {2020},
  abstract  = {The heart is the most energy-consuming organ in the human body. In heart failure, the homeostasis of energy supply and demand is endangered by an increase in cardiomyocyte workload, or by an insufficiency in energy-providing processes. Energy metabolism is directly associated with mitochondrial redox homeostasis. The production of toxic reactive oxygen species (ROS) may overwhelm mitochondrial and cellular ROS defense mechanisms in case of heart failure. Mitochondria are essential cell organelles and provide 95\% of the required energy in the heart. Metabolic remodeling, changes in mitochondrial structure or function, and alterations in mitochondrial calcium signaling diminish mitochondrial energy provision in many forms of cardiomyopathy. The mitochondrial respiratory chain creates a proton gradient across the inner mitochondrial membrane, which couples respiration with oxidative phosphorylation and the preservation of energy in the chemical bonds of ATP. Akin to other mitochondrial enzymes, the respiratory chain is integrated into the inner mitochondrial membrane. The tight association with the mitochondrial phospholipid cardiolipin (CL) ensures its structural integrity and coordinates enzymatic activity. This review focuses on how changes in mitochondrial CL may be associated with heart failure. Dysfunctional CL has been found in diabetic cardiomyopathy, ischemia reperfusion injury and the aging heart. Barth syndrome (BTHS) is caused by an inherited defect in the biosynthesis of cardiolipin. Moreover, a dysfunctional CL pool causes other types of rare inherited cardiomyopathies, such as Sengers syndrome and Dilated Cardiomyopathy with Ataxia (DCMA). Here we review the impact of cardiolipin deficiency on mitochondrial functions in cellular and animal models. We describe the molecular mechanisms concerning mitochondrial dysfunction as an incitement of cardiomyopathy and discuss potential therapeutic strategies.},
  language  = {en}
}
@article{TrinklKaluzaWallaceetal.2020,
  author    = {Trinkl, Moritz and Kaluza, Benjamin F. and Wallace, Helen and Heard, Tim A. and Keller, Alexander and Leonhardt, Sara D.},
  title     = {Floral Species Richness Correlates with Changes in the Nutritional Quality of Larval Diets in a Stingless Bee},
  series = {Insects},
  volume    = {11},
  journal   = {Insects},
  number    = {2},
  issn      = {2075-4450},
  doi       = {10.3390/insects11020125},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-200605},
  pages     = {125},
  year      = {2020},
  abstract  = {Bees need food of appropriate nutritional quality to maintain their metabolic functions. They largely obtain all required nutrients from floral resources, i.e., pollen and nectar. However, the diversity, composition and nutritional quality of floral resources varies with the surrounding environment and can be strongly altered in human-impacted habitats. We investigated whether differences in plant species richness as found in the surrounding environment correlated with variation in the floral diversity and nutritional quality of larval provisions (i.e., mixtures of pollen, nectar and salivary secretions) composed by the mass-provisioning stingless bee Tetragonula carbonaria (Apidae: Meliponini). We found that the floral diversity of larval provisions increased with increasing plant species richness. The sucrose and fat (total fatty acid) content and the proportion and concentration of the omega-6 fatty acid linoleic acid decreased, whereas the proportion of the omega-3 fatty acid linolenic acid increased with increasing plant species richness. Protein (total amino acid) content and amino acid composition did not change. The protein to fat (P:F) ratio, known to affect bee foraging, increased on average by more than 40\% from plantations to forests and gardens, while the omega-6:3 ratio, known to negatively affect cognitive performance, decreased with increasing plant species richness. Our results suggest that plant species richness may support T. carbonaria colonies by providing not only a continuous resource supply (as shown in a previous study), but also floral resources of high nutritional quality.},
  language  = {en}
}
@article{WongPittig2022,
  author    = {Wong, Alex H. K. and Pittig, Andre},
  title     = {A dimensional measure of safety behavior: A non-dichotomous assessment of costly avoidance in human fear conditioning},
  series = {Psychological Research},
  volume    = {86},
  journal   = {Psychological Research},
  number    = {1},
  issn      = {1430-2772},
  doi       = {10.1007/s00426-021-01490-w},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-267688},
  pages     = {312-330},
  year      = {2022},
  abstract  = {Safety behavior prevents the occurrence of threat, thus it is typically considered adaptive. However, safety behavior in anxiety-related disorders is often costly, and persists even the situation does not entail realistic threat. Individuals can engage in safety behavior to varying extents, however, these behaviors are typically measured dichotomously (i.e., to execute or not). To better understand the nuances of safety behavior, this study developed a dimensional measure of safety behavior that had a negative linear relationship with the admission of an aversive outcome. In two experiments, a Reward group receiving fixed or individually calibrated incentives competing with safety behavior showed reduced safety behavior than a Control group receiving no incentives. This allowed extinction learning to a previously learnt warning signal in the Reward group (i.e., updating the belief that this stimulus no longer signals threat). Despite the Reward group exhibited extinction learning, both groups showed a similar increase in fear to the warning signal once safety behavior was no longer available. This null group difference was due to some participants in the Reward group not incentivized enough to disengage from safety behavior. Dimensional assessment revealed a dissociation between low fear but substantial safety behavior to a safety signal in the Control group. This suggests that low-cost safety behavior does not accurately reflect the fear-driven processes, but also other non-fear-driven processes, such as cost (i.e., engage in safety behavior merely because it bears little to no cost). Pinpointing both processes is important for furthering the understanding of safety behavior.},
  language  = {en}
}
@article{DitzelKoenigMusembietal.2022,
  author    = {Ditzel, Pia and K{\"o}nig, Sebastian and Musembi, Peter and Peters, Marcell K.},
  title     = {Correlation between coral reef condition and the diversity and abundance of fishes and sea urchins on an East African coral reef},
  series = {Oceans},
  volume    = {3},
  journal   = {Oceans},
  number    = {1},
  issn      = {2673-1924},
  doi       = {10.3390/oceans3010001},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-284503},
  pages     = {1 -- 14},
  year      = {2022},
  abstract  = {Coral reefs are one of the most diverse marine ecosystems, providing numerous ecosystem services. This present study investigated the relationship between coral reef condition and the diversity and abundance of fishes, on a heavily fished East African coral reef at Gazi Bay, Kenya. Underwater visual censuses were conducted on thirty 50 × 5 m belt transects to assess the abundance and diversity of fishes. In parallel, a 25-m length of each of the same transects was recorded with photo-quadrats to assess coral community structure and benthic characteristics. For statistical analyses, multi-model inference based on the Akaike Information Criterion was used to evaluate the support for potential predictor variables of coral reef and fish diversity. We found that coral genus richness was negatively correlated with the abundance of macroalgae, whereas coral cover was positively correlated with both the abundance of herbivorous invertebrates (sea urchins) and with fish family richness. Similarly, fish family richness appeared mainly correlated with coral cover and invertebrate abundance, although no correlates of fish abundance could be identified. Coral and fish diversity were very low, but it appears that, contrary to some locations on the same coast, sea urchin abundance was not high enough to be having a negative influence on coral and fish assemblages. Due to increasing threats to coral reefs, it is important to understand the relationship among the components of the coral reef ecosystem on overfished reefs such as that at Gazi Bay.},
  language  = {en}
}
@article{DannerKellerHaerteletal.2017,
  author    = {Danner, Nadja and Keller, Alexander and H{\"a}rtel, Stephan and Steffan-Dewenter, Ingolf},
  title     = {Honey bee foraging ecology: Season but not landscape diversity shapes the amount and diversity of collected pollen},
  series = {PLoS ONE},
  volume    = {12},
  journal   = {PLoS ONE},
  number    = {8},
  doi       = {10.1371/journal.pone.0183716},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-170424},
  pages     = {e0183716},
  year      = {2017},
  abstract  = {The availability of pollen in agricultural landscapes is essential for the successful growth and reproduction of honey bee colonies (Apis mellifera L.). The quantity and diversity of collected pollen can influence the growth and health of honey bee colonies, but little is known about the influence of landscape structure on pollen diet. In a field experiment, we rotated 16 honey bee colonies across 16 agricultural landscapes, used traps to collect samples of collected pollen and observed intra-colonial dance communication to gain information about foraging distances. DNA metabarcoding was applied to analyze mixed pollen samples. Neither the amount of collected pollen nor pollen diversity was related to landscape diversity. However, we found a strong seasonal variation in the amount and diversity of collected pollen in all sites independent of landscape diversity. The observed increase in foraging distances with decreasing landscape diversity suggests that honey bees compensated for lower landscape diversity by increasing their pollen foraging range in order to maintain pollen amount and diversity. Our results underscore the importance of a diverse pollen diet for honey bee colonies. Agri-environmental schemes aiming to support pollinators should focus on possible spatial and temporal gaps in pollen availability and diversity in agricultural landscapes.},
  language  = {en}
}
@article{KadeBakirciTandonetal.2022,
  author    = {Kade, Juliane C. and Bakirci, Ezgi and Tandon, Biranche and Gorgol, Danila and Mrlik, Miroslav and Luxenhofer, Robert and Dalton, Paul D.},
  title     = {The Impact of Including Carbonyl Iron Particles on the Melt Electrowriting Process},
  series = {Macromolecular Materials and Engineering},
  volume    = {307},
  journal   = {Macromolecular Materials and Engineering},
  number    = {12},
  issn      = {1438-7492},
  doi       = {10.1002/mame.202200478},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-318482},
  year      = {2022},
  abstract  = {Melt electrowriting, a high-resolution additive manufacturing technique, is used in this study to process a magnetic polymer-based blend for the first time. Carbonyl iron (CI) particles homogenously distribute into poly(vinylidene fluoride) (PVDF) melts to result in well-defined, highly porous structures or scaffolds comprised of fibers ranging from 30 to 50 µm in diameter. This study observes that CI particle incorporation is possible up to 30 wt\% without nozzle clogging, albeit that the highest concentration results in heterogeneous fiber morphologies. In contrast, the direct writing of homogeneous PVDF fibers with up to 15 wt\% CI is possible. The fibers can be readily displaced using magnets at concentrations of 1 wt\% and above. Combined with good viability of L929 CC1 cells using Live/Dead imaging on scaffolds for all CI concentrations indicates that these formulations have potential for the usage in stimuli-responsive applications such as 4D printing.},
  language  = {en}
}
@article{SchmidtSticherlingSardyetal.2020,
  author    = {Schmidt, Enno and Sticherling, Michael and S{\´a}rdy, Mikl{\´o}s and Eming, R{\"u}diger and Goebeler, Matthias and Hertl, Michael and Hofmann, Silke C. and Hunzelmann, Nicolas and Kern, Johannes S. and Kramer, Harald and Nast, Alexander and Orzechowski, Hans-Dieter and Pfeiffer, Christiane and Schuster, Volker and Sitaru, Cassian and Zidane, Miriam and Zillikens, Detlef and Worm, Margitta},
  title     = {S2k guidelines for the treatment of pemphigus vulgaris/foliaceus and bullous pemphigoid: 2019 update},
  series = {JDDG: Journal der Deutschen Dermatologischen Gesellschaft},
  volume    = {18},
  journal   = {JDDG: Journal der Deutschen Dermatologischen Gesellschaft},
  number    = {5},
  doi       = {10.1111/ddg.14097},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-217806},
  pages     = {516 -- 526},
  year      = {2020},
  language  = {en}
}
@techreport{SavvidisRothTutsch2022,
  type      = {Working Paper},
  author    = {Savvidis, Dimitrios and Roth, Robert and Tutsch, Dietmar},
  title     = {Static Evaluation of a Wheel-Topology for an SDN-based Network Usecase},
  series = {W{\"u}rzburg Workshop on Next-Generation Communication Networks (WueWoWas'22)},
  journal   = {W{\"u}rzburg Workshop on Next-Generation Communication Networks (WueWoWas'22)},
  doi       = {10.25972/OPUS-28071},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-280715},
  pages     = {3},
  year      = {2022},
  abstract  = {The increased occurrence of Software-Defined-Networking (SDN) not only improves the dynamics and maintenance of network architectures, but also opens up new use cases and application possibilities. Based on these observations, we propose a new network topology consisting of a star and a ring topology. This hybrid topology will be called wheel topology in this paper. We have considered the static characteristics of the wheel topology and compare them with known other topologies.},
  subject      = {Datennetz},
  language  = {en}
}
@book{OPUS4-18818,
  title     = {MUS-IC-ON! Klang der Antike. Begleitband zur Ausstellung im Martin von Wagner Museum der Universit{\"a}t W{\"u}rzburg 10. Dezember 2019 bis 12. Juli 2020},
  editor    = {Leitmeir, Florian and Shehata, Dahlia and Wiener, Oliver},
  edition   = {1. Auflage},
  publisher = {W{\"u}rzburg University Press},
  address   = {W{\"u}rzburg},
  isbn      = {978-3-95826-122-8},
  doi       = {10.25972/WUP-978-3-95826-123-5},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-188181},
  publisher      = {W{\"u}rzburg University Press},
  pages     = {XIX, 221},
  year      = {2019},
  abstract  = {"MUS-IC-ON! Klang der Antike" ist der Begleitband zur gleichnamigen Sonderausstellung im Martin von Wagner-Museum W{\"u}rzburg (10. Dezember 2019 bis 12. Juli 2020). Die eigene Musik zu verstehen verlangt auch, nach Kl{\"a}ngen und Musik der Vergangenheit zu fragen. So offenbart der Blick auf antike Musikkulturen - des Vorderen Orients, {\"A}gyptens, Griechenlands und Roms - dem Betrachter kulturelle Konstanten, deren Verm{\"a}chtnis sich in unserer abendl{\"a}ndischen Musik erh{\"a}lt. Ziel des Begleitbands zur Ausstellung "MUS-IC-ON! Klang der Antike" ist es, die Relevanz musikhistorischer und -arch{\"a}ologischer Forschung f{\"u}r das Verst{\"a}ndnis der eigenen Musikkultur aufzuzeigen. An vier Schwerpunkten werden die Inhalte, Methoden und der Stand der Erforschung antiker Musik von international namhaften Wissenschaftler*innen in einer umfassenden und dennoch allgemein verst{\"a}ndlichen Weise vorgestellt. Der reichhaltig bebilderte Band ist damit als ein einf{\"u}hrendes und informatives Handbuch konzipiert, das {\"u}ber die Ausstellung hinaus von fachfremden Wissenschaftlern, Studierenden und interessierten Laien konsultiert wird. 1. Vom arch{\"a}ologischen Artefakt zum klingenden Instrument: Grundlage jedweder Erforschung antiker Musik ist die Rekonstruktion seiner Klangvielfalt. Unz{\"a}hlige Funde originaler Musikinstrumente, darunter vorderasiatische Kastenleiern, alt{\"a}gyptische Trompeten aus dem Grab des Tutanchamun oder die griechisch-r{\"o}mische Hydraulis, er{\"o}ffnen einen Weg, den Kl{\"a}ngen antiker Musik nachzugehen. Insgesamt sechs Beitr{\"a}ge mit einem historischen Rahmen, der von der Steinzeit bis in die r{\"o}mische Kaiserzeit reicht, stellen die Vorgehensweise aber auch die Herausforderungen bei der Bergung, Erforschung und dem Nachbau antiker Musikinstrumente vor. 2. Musik und Klang in Bild und Text: Ein umfassendes Bild vom Einsatz, der Spielweise, der Verbreitung und Entwicklung antiker Musikinstrumente liefern antike Bilder und Texte. Trotz der Vielfalt an Objekten, darunter griechische Vasen, assyrische Reliefbilder oder r{\"o}mische Mosaike, bedarf es einer analytischen und kritischen Herangehensweise bei der Analyse und Auswertung solcher Daten. Vorgestellt werden in diesem Themenschwerpunkt auch antike Notationsformen und Stimmungssysteme, die sich auf Papyri und Keilschrifttafeln erhalten haben, sowie die Schwierigkeiten ihrer Entzifferung. 3. Musikleben in der Antike: Wesentlich f{\"u}r das Verst{\"a}ndnis antiker Musik ist die Kenntnis ihres Einsatzes und ihres Umfeldes. Getrennt nach den verschiedenen Kulturkreisen stellen in diesem Kapitel insgesamt neun Autoren unterschiedliche Bereiche und Funktionen des Musizierens vor. Herausragend ist hier die Rolle von Musik im t{\"a}glichen Tempelkult, {\"u}ber die sich die in der Antike vorgestellte Macht von Musik aufzeigen l{\"a}sst, die sogar auf die Gem{\"u}ter der G{\"o}tter Einfluss zu nehmen vermag. Zus{\"a}tzlich aufschlussreich ist das Wissen von Ausbildung und Aufstieg auch namentlich bekannter Musiker, zumeist Hofmusiker, die den Ruhm ihres K{\"o}nigs verk{\"u}nden, aber auch regelrechte Virtuosen, die sich beispielsweise in antiken Wettstreiten hervortun, den musischen Agonen. 4. Kontinuit{\"a}t und Interkulturalit{\"a}t antiker Musikkulturen: Dieser Teil widmet sich Spuren, die antike Musikkulturen in der abendl{\"a}ndischen sowie in orientalischen Kulturr{\"a}umen hinterlassen haben. So geht unser modernes Tonsystem auf griechische, ja sogar auf babylonische Tonleitern zur{\"u}ck. Vorl{\"a}ufer der europ{\"a}ischen Kirchenorgel ist die Hydraulis, eine griechische Erfindung aus dem 3. vorchristlichen Jahrhundert. Viele vor {\"u}ber 4500 Jahren zwischen Euphrat und Tigris entwickelte Musikinstrumente werden noch heute im arabischen und afrikanischen Kulturkreis, ja selbst in nord- und osteurop{\"a}ischen L{\"a}ndern gespielt. Hervorgehoben werden außerdem historische Momente, die einen wesentlichen Einfluss auf die Entwicklung moderner Musiksysteme ausge{\"u}bt haben. Die Autoren: Benedetta Bellucci (Vorderasiatische Arch{\"a}ologie, Mainz), Arndt A. Both (Altamerikanistik, Musikarch{\"a}ologie, DAI Berlin), Ricardo Eichmann (Vorderasiatische Arch{\"a}ologie, DAI Berlin), Uri Gabbay (Altorientalistik, Jerusalem), Ralf Gehler (Instrumentenbauer, Schwerin), Carolin Goll (Martin von Wagner-Museum, W{\"u}rzburg), Jochen Griesbach (Martin von Wagner-Museum, Klassische Arch{\"a}ologie, W{\"u}rzburg), Stefan Hagel (Klassische Philologie, {\"O}AW Wien), Katharina Hepp ({\"A}gyptologie, W{\"u}rzburg), Peter Holmes (Instrumentenbauer, London), Marie Klein (Altorientalisik, W{\"u}rzburg), Carola Koch {\"A}gyptologie, W{\"u}rzburg), Ulrich Konrad (Historische Musikwissenschaft, W{\"u}rzburg), Eva Kurz ({\"A}gyptologie W{\"u}rzburg), Florian Leitmeir (Klassische Arch{\"a}ologie, W{\"u}rzburg), Thomas Ludewig (Klassische Arch{\"a}ologie, W{\"u}rzburg), Sam Mirelman (Altorientalistik, London), Daniel Schwemer (Altorientalistik, W{\"u}rzburg), Dahlia Shehata (Altorientalisik, W{\"u}rzburg), Martin Stadler ({\"A}gyptologie, W{\"u}rzburg), Olga Sutkowska (Kulturgeschicht der Antike, {\"O}AW Wien), G{\"u}nther E. Th{\"u}ry (Provinzialr{\"o}mische Arch{\"a}ologie, Salzburg), Marc Wahl (Numismatik, Wien/W{\"u}rzburg), Oliver Wiener (Historische Musikwissenschaft, W{\"u}rzburg), Nele Ziegler (Altorientalistik, Paris)},
  subject      = {Martin-von-Wagner-Museum. {\"A}ltere Abteilung},
  language  = {de}
}
@book{Arnold2018,
  author    = {Arnold, Irina},
  title     = {Hunde auf ihrem Weg durch EUropa. Ethnographische Einblicke in den Tierschutz zwischen Spanien und Deutschland.},
  doi       = {10.25972/OPUS-17219},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-172193},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  pages     = {156},
  year      = {2018},
  abstract  = {Wie funktioniert Tierschutz im Alltag? Basierend auf Interviews und Feldforschung mit einem Verein, der Hunde aus spanischen T{\"o}tungsstationen nach Deutschland holt und in neue Familien vermittelt, wird den vielf{\"a}ltigen Aspekten dieser Frage aus Sicht der Europ{\"a}ischen Ethnologie nachgegangen. Das Beispiel des Welpen „Hugo", der w{\"a}hrend der Forschung gefunden wurde, veranschaulicht die allt{\"a}glichen Praktiken der Tierschutzarbeit. Es dient außerdem als Ausgangspunkt f{\"u}r die Beschreibung der komplexen Netzwerke, in die der Verein und seine Akteur_innen eingebunden sind. Netzwerke, hier konzeptualisiert als Assemblages, bestehen nicht nur aus Menschen. Sie umfassen ebenfalls nichtmenschliche Tiere, Gesetze, Dinge, Institutionen, Diskurse und Erz{\"a}hlungen. Die qualitativen ethnographischen Einblicke in das Allt{\"a}gliche der Tierschutzarbeit werden gerahmt, begleitet und gest{\"u}tzt durch Ausf{\"u}hrungen zur Tierschutz- und Tierrechtsgeschichte. Außerdem gibt die Arbeit einen {\"U}berblick {\"u}ber Perspektiven auf nichtmenschliche Tiere aus verschiedenen wissenschaftlichen Disziplinen wie der Philosophie, den Natur- und Rechtswissenschaften. Die Bereicherungen einer europ{\"a}isch-ethnologisch ausgerichteten Forschung, die mit und nicht nur {\"u}ber andere Lebewesen forscht, werden in dieser Arbeit herausgestellt.},
  subject      = {Tierschutz},
  language  = {de}
}
@book{JobFliessbachSchendzielorzBittlingmaieretal.2019,
  author    = {Job, Hubert and Fließbach-Schendzielorz, Maike and Bittlingmaier, Sarah and Herling, Anne and Woltering, Manuel},
  title     = {Akzeptanz der bayerischen Nationalparks - Ein Beitrag zum sozio{\"o}konomischen Monitoring in den Nationalparks Bayerischer Wald und Berchtesgaden},
  edition   = {1. Auflage},
  publisher = {W{\"u}rzburg University Press},
  address   = {W{\"u}rzburg},
  isbn      = {978-3-95826-102-0},
  issn      = {0510-9833},
  doi       = {10.25972/WUP-978-3-95826-103-7},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-171246},
  publisher      = {W{\"u}rzburg University Press},
  pages     = {XI, 149},
  year      = {2019},
  abstract  = {Nationalparks sind das {\"a}lteste und bekannteste fl{\"a}chenbezogene Naturschutzinstrument weltweit. F{\"u}r den Erhalt einer nachhaltigen Lebensgrundlage und die Entwicklung der Biodiversit{\"a}t sowie f{\"u}r mehr Naturdynamik in der Landschaft haben sie eine sehr große Bedeutung, auch in unseren Breiten. Dennoch ist die Einstellung zu Nationalparks von Seiten der unmittelbaren Anwohner nicht immer unproblematisch. Entsprechend versucht die vorliegende wissenschaftliche Analyse neue Erkenntnisse bez{\"u}glich der Akzeptanz der Nationalparks Bayerischer Wald und Berchtesgaden, den {\"a}ltesten Deutschlands, aufzuzeigen. Empirische Grundlagen f{\"u}r diese Studie sind eine bayernweite Online-Befragung, qualitative Experteninterviews und aufw{\"a}ndige repr{\"a}sentative schriftliche Befragungen in den Nationalpark-Landkreisen Regen und Freyung-Grafenau bzw. Berchtesgadener Land im Jahr 2018. Auch die zeitliche Entwicklung der Akzeptanz wird auf Basis der Ergebnisse von Vorg{\"a}ngerstudien, soweit m{\"o}glich, ber{\"u}cksichtigt. Dabei sind es {\"o}konomische, emotionale, interpersonelle, soziokulturelle und nicht zuletzt f{\"u}r Geographen besonders interessante raumzeitliche Pr{\"a}diktoren der Akzeptanz beider Nationalparks, die im Fokus der Untersuchungen stehen.},
  subject      = {Nationalpark Bayerischer Wald},
  language  = {de}
}
@book{StaffeldtThieroff2020,
  author    = {Staffeldt, Sven and Thieroff, Rolf},
  title     = {Oberfl{\"a}chengrammatischer Wald : Syntaktische Baumschule am Beispiel eines authentischen Textes},
  edition   = {1. Auflage},
  isbn      = {978-3-945459-31-7},
  doi       = {10.25972/OPUS-20561},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-205614},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  pages     = {150},
  year      = {2020},
  abstract  = {When analysing a sentence, you are revealing your understanding of this sentence by certain criteria. Before analysing a sentence, you need to understand it in a basic, pre-analytical, concealed way: Analysis is not possible without understanding. The process of analysing a sentence will concretize and finalize this understanding. You will need a theoretical background, the theoretical "tools" when uncovering your syntactical understanding of a sentence by means of syntactical analysis: Analysis is not possible without these theoretical tools. The tools used in the theoretical framework of surface grammar (Oberfl{\"a}chengrammatik) as they were first elaborated by Eisenberg in his 1986 grammar book include syntactic constituents such as verb, noun, noun group and the like (syntactic forms), and syntactic relations, such as predicate, subject, object and the like (syntactic functions). While this sounds like a rather traditional view of grammar, the thorough analyses presented here show that there is more to surface grammar than one might expect. The prime goal of this publication is to present the results of different sentence analyses in form of syntactical trees with information about the forms and functions of the syntactic constituents used. You will find more than 100 syntactical trees, forming a "syntactical forest". It gives students (and other readers) who are analysing sentences in the framework of surface grammar an opportunity to test their knowledge and understanding of certain syntactical phenomena against the background of authentic use of speech. Besides, the "Surface Grammar in action" is proof of the soundness of the analytical methods and tools used by appropriately analysing linguistic phenomena found in the wilderness of journalistic text production. And yet, sometimes there is more than one solution, more than just one way to explain the structures found. Most authors decide which road to take by themselves. We include the crossways by adding our dialogues about arguable cases, so that readers may see several options of how to explain the more difficult (and more interesting) aspects of grammar. The present publication appears in the year of Peter Eisenberg's 80th birthday and is dedicated to him with the deepest respect for his life work and especially for the foundation of a syntactic framework with which so many generations of university students are working and which has helped so many understand the intriguing features of the grammar of the German language.},
  subject      = {Grammatik},
  language  = {de}
}
@article{ZieglerPollingerBoelletal.2020,
  author    = {Ziegler, Katrin and Pollinger, Felix and B{\"o}ll, Susanne and Paeth, Heiko},
  title     = {Statistical modeling of phenology in Bavaria based on past and future meteorological information},
  series = {Theoretical and Applied Climatology},
  volume    = {140},
  journal   = {Theoretical and Applied Climatology},
  issn      = {0177-798X},
  doi       = {10.1007/s00704-020-03178-4},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232717},
  pages     = {1467-1481},
  year      = {2020},
  abstract  = {Plant phenology is well known to be affected by meteorology. Observed changes in the occurrence of phenological phases arecommonly considered some of the most obvious effects of climate change. However, current climate models lack a representationof vegetation suitable for studying future changes in phenology itself. This study presents a statistical-dynamical modelingapproach for Bavaria in southern Germany, using over 13,000 paired samples of phenological and meteorological data foranalyses and climate change scenarios provided by a state-of-the-art regional climate model (RCM). Anomalies of severalmeteorological variables were used as predictors and phenological anomalies of the flowering date of the test plantForsythiasuspensaas predictand. Several cross-validated prediction models using various numbers and differently constructed predictorswere developed, compared, and evaluated via bootstrapping. As our approach needs a small set of meteorological observationsper phenological station, it allows for reliable parameter estimation and an easy transfer to other regions. The most robust andsuccessful model comprises predictors based on mean temperature, precipitation, wind velocity, and snow depth. Its averagecoefficient of determination and root mean square error (RMSE) per station are 60\% and ± 8.6 days, respectively. However, theprediction error strongly differs among stations. When transferred to other indicator plants, this method achieves a comparablelevel of predictive accuracy. Its application to two climate change scenarios reveals distinct changes for various plants andregions. The flowering date is simulated to occur between 5 and 25 days earlier at the end of the twenty-first century comparedto the phenology of the reference period (1961-1990).},
  language  = {en}
}
@article{LangePohlmannRother2020,
  author    = {Lange, Sarah D{\´e}sir{\´e}e and Pohlmann-Rother, Sanna},
  title     = {{\"U}berzeugungen von Grundschullehrkr{\"a}ften zum Umgang mit nicht-deutschen Erstsprachen im Unterricht},
  series = {Zeitschrift f{\"u}r Bildungsforschung},
  volume    = {10},
  journal   = {Zeitschrift f{\"u}r Bildungsforschung},
  issn      = {2190-6890},
  doi       = {10.1007/s35834-020-00265-4},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232705},
  pages     = {43-60},
  year      = {2020},
  abstract  = {Mit der vorliegenden Studie werden die {\"U}berzeugungen von berufst{\"a}tigen Grundschullehrkr{\"a}fte zum Umgang mit nicht-deutschen Erstsprachen im Unterricht untersucht und die Bedeutung formaler und informeller bzw. non-formaler Lerngelegenheiten f{\"u}r diese {\"U}berzeugungen analysiert. Die Ergebnisse der Fragebogenstudie (N = 123) zeigen, dass die Mehrheit der befragten Lehrkr{\"a}fte davon {\"u}berzeugt ist, dass Erstsprachen zumindest zeitweise einen Platz in ihrem Unterricht haben sollten. So stimmen beispielsweise 75 \% der befragten Lehrkr{\"a}fte der Aussage mindestens teilweise zu, dass die Sch{\"u}lerinnen und Sch{\"u}ler von Zeit zu Zeit Lerninhalte in ihren Erstsprachen besprechen d{\"u}rfen. Dem Einsatz von nicht-deutschen Lernmaterialien im Unterricht stehen knapp 60 \% der Lehrkr{\"a}fte mindestens positiv gegen{\"u}ber. Die multiplen Regressionsanalysen zeigen, dass sich die erstsprachenintegrierenden {\"U}berzeugungen anhand der Nutzung von formalen und informellen bzw. non-formalen Lerngelegenheiten teilweise erkl{\"a}ren lassen. Eine formale Aus- und Weiterbildung im Themenbereich des Deutschen als Zweitsprache hat einen positiven Einfluss auf die {\"U}berzeugungen von Grundschullehrkr{\"a}ften zum Einbezug von Erstsprachen in ihren Unterricht. Zudem sind es auch informelle bzw. non-formale Sprachkontakte (wie etwa Alltagskommunikation oder Mediennutzung in einer Fremdsprache), deren Nutzung die {\"U}berzeugungen der Grundschullehrkr{\"a}fte hinsichtlich des Umgangs mit Erstsprachen im Unterricht beeinflussen. Unterricht beeinflussen},
  language  = {de}
}
@article{MoratinThoeleLangetal.2023,
  author    = {Moratin, Helena and Th{\"o}le, Anna and Lang, Josephine and Ehret Kasemo, Totta and St{\"o}th, Manuel and Hagen, Rudolf and Scherzad, Agmal and Hackenberg, Stephan},
  title     = {Ag- but not ZnO-nanoparticles disturb the airway epithelial barrier at subtoxic concentrations},
  series = {Pharmaceutics},
  volume    = {15},
  journal   = {Pharmaceutics},
  number    = {10},
  issn      = {1999-4923},
  doi       = {10.3390/pharmaceutics15102506},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-357403},
  year      = {2023},
  abstract  = {Inhalation is considered to be the most relevant source of human exposure to nanoparticles (NPs); however, only a few investigations have addressed the influence of exposing the respiratory mucosal barrier to subcytotoxic doses. In the nasal respiratory epithelium, cells of the mucosa represent one of the first contact points of the human organism with airborne NPs. Disruption of the epithelial barrier by harmful materials can lead to inflammation in addition to potential intrinsic toxicity of the particles. The aim of this study was to investigate whether subtoxic concentrations of zinc oxide (ZnO)- and silver (Ag)-NPs have an influence on upper airway barrier integrity. Nasal epithelial cells from 17 donors were cultured at the air-liquid interface and exposed to ZnO- and Ag-NPs. Barrier function, quantified by transepithelial electrical resistance (TEER), decreased after treatment with 10 µg/mL Ag-NPs, but FITC-dextran permeability remained stable and no change in mRNA levels of tight junction proteins and E-cadherin was detected by real-time quantitative PCR (RT-qPCR). The results indicate that subtoxic concentrations of Ag-NPs may already induce damage of the upper airway epithelial barrier in vitro. The lack of similar disruption by ZnO-NPs of similar size suggests a specific effect by Ag-NPs.},
  language  = {en}
}