@article{StopperMetzler1991,
  author    = {Stopper, Helga and Metzler, M.},
  title     = {Carcinogenic oestrogens induce respiration deficiency mutation in yeast},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63466},
  year      = {1991},
  abstract  = {In addition to hormonal activity, genetic darnage has been proposed as an important factor in oestrogen-mediated carcinogenesis. However, as short-term tests for oestrogens usually fail to show DNA mutations, lesions other than dassie nuclear DNA mutation have to be considered. Oestrogeninduced mitochondrial darnage was studied in the yeast Saccharomyces cerevisiae. Stilbene-type, but not steroidal, oestrogens were found to induce respiration-dcficient petite mutation. The effect was inversely correlated with cytotoxicity and required aromatic hydroxyl groups at the stilbene molecule. It only occurred under growth conditions and apparently was not due to the A TPase inhibitory qualities of stilbene oestrogens. Other studies have shown that petite mutation clones, which can be induced by a variety of substances, contain altered mitochondrial DNA. The mechanism of petite mutation induction might be important in tumorigenesis by also acting on nuclear DNA or facilitating carcinogenesis by disturbance of mitochondrial function.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{CarrSchneider1991,
  author    = {Carr, Martha and Schneider, Wolfgang},
  title     = {Long-term maintenance of organizational strategies in kindergarten children},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62157},
  year      = {1991},
  abstract  = {The goal of the present study was to determine whether 4- and 5-year-old kindergarten children could be trained to maintain an organizational strategy over 2- and 8 week periods through an elaborate training program. A second goal was to assess the effects of the training program on strategy awareness. Twenty-eight kindergarten children were pretested on two sort-recall tasks and their awareness of the use of the clustering strategy was assessed through a protocol type procedure. Half the children received seven half-hour sessions of individual training in the clustering strategy and half the children participated in a control group. Both groups were post-tested on two sort-recall tasks 2 weeks following training and again 8 weeks following training. Strategy awareness, as measured by verbal protocol, was assessed at both post-test points. The elaborate strategy training program was successful in inducing short- and long-term strategy maintenance of the clustering strategy. Trained children's clustering during sorting and clustering during recall was consistently related to the amount of items correctly recalled. No differences in strategy awareness were found. These findings demonstrate that the elaborate training procedure used in this study can be a very effective memory technique for young kindergarten children.},
  subject      = {Psychologie},
  language  = {en}
}
@article{TasStopperKoscheletal.1991,
  author    = {Tas, P. and Stopper, Helga and Koschel, K. and Schiffmann, D.},
  title     = {Influence of the carcinogenic oestrogen diethylstilboestrol on the intracellular calcium level in C6 rat glioma cells},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63459},
  year      = {1991},
  abstract  = {The ~fthetic oes~rog~n diethylsti~boestrol (DES) causes a dose-dependent elevation of the cytoplasuuc Ca concentratton m C6 rat ghoma cells. This Ca2+ rise is caused neither by Ca2+ influx nor ~-r release from the ~a2 + stores of the endoplasmic reticulum. Therefore it seems likely that DES mob!hzes Ca2+ from a nutochondrial source. The DES-induced Ca2+ signal is remarkably similar to the one mduced by the. tumou~ promotor ~hapsigargin. As this compound causes leakage of calcium from the endoplasmt~ rettculum tt ~ms posstble that DES induces a similar leakage from mitochondrial Ca2+ stores. It remaans to be estabhshed whether the DES-mediated rise in intracellular calcium is causally related to the tumour-promoting properties of this compound},
  subject      = {Toxikologie},
  language  = {en}
}
@article{HackerRdestWintermeyeretal.1991,
  author    = {Hacker, J{\"o}rg and Rdest, Ursula and Wintermeyer, E. and Ludwig, B.},
  title     = {Legiolysin, a New Hemolysin from L. pneumophila},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-73070},
  year      = {1991},
  abstract  = {Legionella pneumophila generares exotoxins, cytolysins, proteases oc hemolysins that darnage host cells llke erythrocytes or rissue cu lrure cells. The gene for a new L. pneumophila hemolysin withour a proteolytic activiry was idemified, cloned in E. coli and sequenced. The gene producr was analysed by SDS-Polyacrylamide-gel-electrophoresis.},
  subject      = {H{\"a}molysin},
  language  = {en}
}
@article{LutzPoetzschSchlatteretal.1991,
  author    = {Lutz, Werner K. and Poetzsch, J. and Schlatter, J. and Schlatter, C.},
  title     = {The real role of risk assessment in cancer risk management},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60730},
  year      = {1991},
  abstract  = {Rtgulatory aclio11s Iaken to reduu tht risk of harmfultffects of exposure to chemieals ofltn arenot commensurDtt with the toxicologicDf risk SJsstS\&ment. A numbtr of factors relating to psychology, sociology, economics Dntl politics rather than science and medicine afftct tht final decision. Wemer Lutz and colleagues illustratt the situation using tht feuktmia-indudng chtmiCJJI benzene as an examplt.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{KrzymanskiWaagaUlrichsetal.1991,
  author    = {Krzymanski, Maciej and Waaga, Ana M. and Ulrichs, Karin and Deja, Aadam and Oko, Andrzej and Rommel, Thomas and M{\"u}ller-Ruchholtz, Wolfgang},
  title     = {The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64431},
  year      = {1991},
  abstract  = {To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.},
  subject      = {Chirurgie},
  language  = {en}
}
@article{SchneiderSchauliesKrethHofmannetal.1991,
  author    = {Schneider-Schaulies, Sibylle and Kreth, H. W. and Hofmann, G. and Billeter, M. A. and ter Meulen, V.},
  title     = {Expression of measles virus RNA in peripheral blood mononuclear cells of patients with measles, SSPE, and autoimmune diseases},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62297},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Virologie},
  language  = {en}
}
@article{KrausSchneiderSchauliesMiyasakaetal.1991,
  author    = {Kraus, E. and Schneider-Schaulies, Sibylle and Miyasaka, M. and Tamatani, T. and Sedgwick, J.},
  title     = {Augmentation of major histocompatibility complex class I and ICAM-1 expression on glial cells following measles virus infection: evidence for the role of type-1 interferon},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62301},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Virologie},
  language  = {en}
}
@article{FeuersteinZerbeSiren1991,
  author    = {Feuerstein, G. and Zerbe, R. L. and Sir{\´e}n, Anna-Leena},
  title     = {Supraoptic nuclei in vasopressin and hemodynamic responses to hemorrhage in rats},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63057},
  year      = {1991},
  abstract  = {CARDIOVASCULAR and vasopressin (A VP) responses to hcmorrhagc wcrc studicd in rats with lesions of the hypothalamic supraoptic nuclei (SONL). Bleeding caused hypotension and increase in heart rate (HR) and A VP. SONL rats failed to fully recover from bleeding as compared to normal rats. Plasma A VP in SONL rats was in the normal in basal conditions, but failed to increase to levels attained in normal rats throughout the post-hemorrhage period. These data suggcst that the supraoptic nuclei are the primary regulatory sitcs for A VP release in rcsponse to hemorrhage and that lack of adequate A VP release significantly retards blood pressure recovery after bleeding.},
  subject      = {Neurobiologie},
  language  = {en}
}
@article{SirenFeuerstein1991,
  author    = {Sir{\´e}n, Anna-Leena and Feuerstein, G.},
  title     = {Hypothalamic opioid µ-receptors regulate discrete hemodynamic functions in the conscious rat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63069},
  year      = {1991},
  abstract  = {The effect of the selective \(\mu\)-opioid agonist o-Ala\(^2\)-Me-Phe\(^4\)-Gly-ol'-enkephalin (DAGO), injected into the medial preoptic nucleus of hypothalamus, on cardiac output and regional blood flow was studied in the conscious rat and the effect of DAGO on renal sympathetic nerve activity and renal blood flow was studied in anesthetized rats. In conscious rats, injections of DAGO (1 or 10 nmol) into the preoptic nucleus increased the blood pressure in a dose-related manner. The maximum rises of mean arterial pressure and pulse pressure after the larger dose were +23 ± 5 mmHg (mean ±SEM, P < 0.01) and + 17 ± 3 mmHg(P < 0.01), respectively. A small dose (0.1 nmol) increased heart rate ( +47 ± 13 bpm, P < 0.05); thc 1 nmol dosc produced bradycardia (- 39 ± 11 bpm, P < 0.05), while the 10 nmol dose initially decreased heart rate ( -68 ± 15 bpm (P < 0.01) and then gradually increased heart rate to a maximum of + 74 ± 13 bpm, (P < 0.0 1). A long-lasting increase in cardiac output was also elicited by DAGO, with maximum changes after 1 and 10 nmol of + 14 ± 6\% and +22 ± 7\% (P < 0.01), respectively. B1ood flow in the hindquarters increascd after DAGO but the mesenteric and renal blood ftow decreased in a dose-related manner. Significant responscs in hindquarter and mesenteric blood fl.ow after DAGO were independent of systemic hemodynamic responses at the dose ofO.l nmol. The vascular resistance in the hindquarters significantly decreased after a small dose of DAGO while the larger doses dose-dependently increased mesenteric and renal vascular resistance. A crucial role of the sympathetic nervous system in the hemodynamic effects of DAGO was demonstrated: (1) by the profound activation of renal sympathetic nerve activity after injections of DAGO (I nmol/100 nl) into the preoptic nucleus, (2) by blockade of the pressor, tachycardic and regional hemodynamic effects of DAGO (I nmol) by the ganglion blocker ch1orisondamine (5 mg/kg i.v.). The results suggest that the pressor effect of DAGO in preoptic nucleus is due primarily to an increase in cardiac output. The differential changes in blood ftow in organs further suggest that the opioid \(\mu\)-receptors in the preoptic nucleus might be involved in the integration of peripheral blood ftow in the hypothalamus during affective behavior.},
  subject      = {Neurobiologie},
  language  = {en}
}
@article{VonhofSirenFeuerstein1991,
  author    = {Vonhof, S. and Sir{\´e}n, Anna-Leena and Feuerstein, G.},
  title     = {Central ventilatory effects of thyrotropin-releasing hormone in the conscious rat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63075},
  year      = {1991},
  abstract  = {Thyrotropin-releasing hormonewas shown to exert potent ventilatory effects after centrat administration. These data, however, were derived from studies using anesthetized animal preparations. Since TRH elicits strong arousal reactions, the observed ventilatory effects of TRH under anesthesia may have been due to nonspecific reduction in the anesthetic state of the animals. In order to clarify the extent to which the reversal of anesthesia may change ventilatory parameters after TRH application, we investigated the effect of TRH on Ventilation rate, relative tidal volume, relative respiratory minute volume, CO\(_2\) production CO\(_2\) consumption, and locomotor activity in the conscious, unrestrained rat. Intracerebroventricular application of TRH induced a dose-dependent, sustained increase in ventilation rate, relative tidal volume, and relative respiratory minute volume of maximally 128\%, 890\%, and 235\%, respectively. In addition, CO\(_2\) production and O\(_2\) consumption were elevated by 4.6 and 11.7 fold, whiJe no significant changes in locomotor activity were observed. The results suggest that TRH stimulates ventilation by a mechanism independent of its analeptic properties.},
  subject      = {Neurobiologie},
  language  = {en}
}
@article{AdeyemoShapiraTombaccinietal.1991,
  author    = {Adeyemo, O. M. and Shapira, S. and Tombaccini, D. and Pollard, H. and Feuerstein, G. and Sir{\´e}n, Anna-Leena},
  title     = {A goldfish model for evaluation of the neurotoxicit of \(\omega\)-conotoxin GVIA and screening of monoclonal antibodies},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63087},
  year      = {1991},
  abstract  = {A Goldfish Model for Evaluation of the Neurotaxicity of \(\omega\)-Conotoxin GVI A and Screening of Monoclonal Antibodies. ADEYEMO, 0. M .. SHAPIRA, S., TOMBACCINI, D., POLLARD, H. 8 .• FEUERSTEIN, G .. AND SIREN, A-L. ( 1991 ). Toxicol. App/. Pharmaco/. 108, 489-496. The neurotoxicity of \(\omega\)-conotoxin (\(\omega\)-CgTx), a potent neuronal voltage-sensitive calcium channel blocker, was measured using a new bioassay. \(\omega\)-CgTx was administered intraperitoneally (ip) to goldfish weighing approximately 1.6 g, and dose-related changes were observed over a 2-hr period. \(\omega\)CgTx induced time- and dose-dependent abnormal swimming behavior (ASB) and mortality. The antitoxin activity of the antiborlies was investigated in vivo by either ( l) preincubation of the antibody with w-CgTx at 4°C overnight, or (2) pretreatment with antibody, 30 min before \(\omega\)CgTx injection in a 10:1 antibody/\(\omega\)-CgTx molar ratio. The LD50 dose of \(\omega\)-CgTx in goldfish was 5 nmol/kg ip, and preincubation of monoclonal antibody (50 nmol/kg ip) with \(\omega\)-CgTx (5 nmol/kg ip) significantly (p < 0.05) reduced mortality. ASB, and toxicity time. The antitoxin activity of the monoclonal antiborlies evidenced in the goldfish bioassay was further tested in the conscious rat. In the rat, the increases in mean arterial pressure and heart rate induced by \(\omega\)-CgTx (0.03 nmol/rat icv) were significantly (p < 0.02 and p < 0.0 l, respectively) attenuated by preincubation of the toxin with the antibody (0.3 nmol/rat). We conclude that the goldfish bioassay provides a simple. accurate, and inexpensive in vivo model for the study of the toxicity of \(\omega\)CgTx},
  subject      = {Neurobiologie},
  language  = {en}
}
@article{SirenVonhofFeuerstein1991,
  author    = {Sir{\´e}n, Anna-Leena and Vonhof, S. and Feuerstein, G.},
  title     = {Hemodynamic defense response to thyrotropin-releasing hormone injected into medial preoptic nucleus in rats},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63099},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Neurobiologie},
  language  = {en}
}
@techreport{OPUS4-4202,
  title     = {Universit{\"a}tsbibliothek W{\"u}rzburg : Bericht {\"u}ber die Gesch{\"a}ftsjahre 1978 - 1989},
  organization    = {Universit{\"a}tsbibliothek W{\"u}rzburg},
  isbn      = {3-923959-17-6},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-50616},
  year      = {1991},
  abstract  = {Die Entwicklung der Universit{\"a}tsbibliothek W{\"u}rzburg in den Jahren 1978 bis 1989.},
  subject      = {W{\"u}rzburg},
  language  = {de}
}
@article{MuellerStahlHennigRethwilmetal.1991,
  author    = {M{\"u}ller, J. G. and Stahl-Hennig, Christiane and Rethwilm, Axel and Kneitz, C. and Kerkau, Thomas and Schmauser, B. and Schindler, C and Krenn, V. and terMeulen, V. and M{\"u}ller-Hermelink, H.K.},
  title     = {Morphologische Untersuchungen von Lymphknoten und Thymusin der Fr{\"u}hphase der SIV-Infektion bei Rhesus-Affen},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47183},
  year      = {1991},
  abstract  = {Rhesus monkeys (M. mulatta) were i. v. infected with SIV mac251. Three phases of lymph node changes were observed. 1: physiological follicular hyperplasia (3 and 6 weeks p.i.). 2: Alterations of germinal centers: loss of follicular mantle zone, fragmentation or sclerosis (12 and 24 weeks p.i.). 3: Partial depletion of T-lymphocytes, accumulation of plasma cells, increased numbers of syncytial giant cells, hemophgocytosis in the sinuses (about 1 year p.i.). The thymus of the juvenile animals showed first changes 12 and 24 weeks after infection with focalloss of immature (and Ki-67 positive) cortical thymocytes, leading to severe accidental involution of the thymuses one year after infection and reduced numbers of Hassalls corpuscles. These investigations show the value of this animal model for the study of morphology and pathogenesis of AIDS.},
  subject      = {Affenimmundefizienzvirus},
  language  = {de}
}
@article{Wilhelm1991,
  author    = {Wilhelm, Gernot},
  title     = {Zur hurritischen Gebetsliteratur},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-51127},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Koch},
  language  = {de}
}
@article{RethwilmErlweinBaunachetal.1991,
  author    = {Rethwilm, Axel and Erlwein, Otto and Baunach, Gerald and Mauerer, Bernd and ter Meulen, Volker},
  title     = {The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47342},
  year      = {1991},
  abstract  = {The human foamy virus (HFV) genome possesses three open reading frames (bel I, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most Iikely candidate to encode the viral transactivator. ResuIts presented here confirmed this and showed further that a deletion introduced only into the bell open reading frame of a plasmid derived from an infectious molecular clone of HFV abolished transactivation. In contrast, deletions in bel 2 and bel 3 had only minor effects on the ability to transactivate. The role of the bel I genomic region as a transactivator was further investigated by eukaryotic expression of a genome fragment of HFV spanning the bel I open reading frame. A construct expressing bell under control of a heterologous promoter was found to transactivate the HFV long terminal repeat in a dose-dependent fashion. Furthermore, it is shown that the U3 region of the HFV long terminal repeat is sufficient to respond to the HFV transactivator.},
  subject      = {Virologie},
  language  = {en}
}
@article{SchneiderSchauliesKirchhoffArchelosetal.1991,
  author    = {Schneider-Schaulies, J{\"u}rgen and Kirchhoff, F. and Archelos, J. and Schachner, M.},
  title     = {Downregulation of Myelin Associated Glycoprotein (MAG) on Schwann cells by interferon-gamma and tumor necrosis factor-alpha affects neurite outgrowth},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54850},
  year      = {1991},
  abstract  = {To investigate the influence of inflammatory cytokines on the potential of peripheral nerves to regenerate, we analyzed the effect of interferon-y (lFN-y) and tumor necrosis factor-a (TNF-a) on the ability of immortalized Schwann cells to mediate outgrowth of neurites from primary DRG neurons. We found that IFN-y and TNF-a synergistically inhibited the neurite outgrowth-promoting properties of the Schwann cells by spedfically dowllregulating myelin-associated glycoprotein (MAG) at the levels of mRNA and cell surface protein by approximately 60\%. Antibodies to MAG inhibited the outgrowth of neurites on Schwann cells to the same extent as treatment with the two cytokines. Since MAG appears to be involved in both neurite outgrowth and myelination, our findings may provide evidence for a mechanism, by wh ich inflammatory cytokines interfere with Schwann cell-neuron interactions.},
  subject      = {Immunologie},
  language  = {en}
}
@inproceedings{FialaMaschwitzTho1991,
  author    = {Fiala, Brigitte and Maschwitz, Ulrich and Tho, Yow Pong},
  title     = {The association between Macaranga trees and ants in South-east Asia},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54752},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Macaranga},
  language  = {de}
}
@article{VonhofSiren1991,
  author    = {Vonhof, S. and Sir{\´e}n, Anna-Leena},
  title     = {Reversal of µ-opioid-mediated respiratory depression by α2-adrenoceptor antagonism},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47454},
  year      = {1991},
  abstract  = {The present study was performed in order to evaluate the effects of the selective 02- adrenoceptor antagonist 6-chloro-2,3,4,5-tetrahydro-3-methyl-1H-3-benzazepine (SK\&F 86466) on dermorphin-induced analgesia, respiratory depression and inhibition of locomotor activity in the conscious rat. Intracerebroventricular (icv) administration of dermorphin (3 nmol/rat) decreased respiration rate and relative ventilatory minute volume maximally by 38 \% and 50 \% of baseline respectively. SK\&F 86466 dose-dependently reversed the dermorphin-induced depression of ventilatory parameters, while SK\&F 86466 exerted no effect on dermorphin-induced analgesia or depression of locomotor activity due to catalepsia. It appears, therefore, that a 2-adrenoceptors selectively interact with Jl2-opioid-receptor mediated effects, such as respiratory depression, but are not involved in the modulation of Jl,-opioid-related effects, such as supraspinal analgesia and depression of locomotor activity.},
  subject      = {Biowissenschaften},
  language  = {en}
}
@article{SolbachMollRoellinghoff1991,
  author    = {Solbach, Werner and Moll, Heidrun and R{\"o}llinghoff, Martin},
  title     = {Lymphocytes play the music but the macrophage calls the tune},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45889},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Immunologie},
  language  = {en}
}
@article{PfefferSchoelGulleetal.1991,
  author    = {Pfeffer, K. and Schoel, H. and Gulle, H. and Moll, Heidrun and Kromer, S. and Kaufmann, S. H. E. and Wagner, H.},
  title     = {Analysis of primary T cell responses to intact and fractionated microbial pathogens},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46916},
  year      = {1991},
  abstract  = {Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacteriallysates, 2 dimensional (2D) PAGE separated mycobacteriallysates, leishmania and defined leishmanial antigen preparations. While,o T cells proliferated vigourously in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates was not detected. In addition '10 T cells failed to respond towards leishmania or leishmanial components. In the ab T cell compartment some donors, presumably according to their state of immunity against mycobacteria, responded to mycobacteria, mycobacterial lysates and 2 D gel separated mycobacterial lysates. Neither freshly isolated '10 T cells nor ab T cells from naive donors did mount a significant immune response against leishmania.},
  language  = {en}
}
@inproceedings{EcksteinUlrichsMeinckeetal.1991,
  author    = {Eckstein, V. and Ulrichs, Karin and Meincke, G. and M{\"u}lller-Ruchholtz, W.},
  title     = {Transplantation of Diabetic Patients With Xenogeneic Pancreatic Islets Has to Consider Natural Xenophile Antibodies (NXA) In Patients Sera As a Major Obstacle to Success},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45707},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Immunbiologie},
  language  = {en}
}
@inproceedings{WinotoMorbachUlrichsMuellerRuchholtz1991,
  author    = {Winoto-Morbach, S. and Ulrichs, Karin and M{\"u}ller-Ruchholtz, W.},
  title     = {New Developments in Biodegradable Microspheres For Magnetic Separation Techniques},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45712},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Immunbiologie},
  language  = {en}
}
@inproceedings{KaitschickUlrichsMuellerRuchholtz1991,
  author    = {Kaitschick, J. and Ulrichs, K. and M{\"u}ller-Ruchholtz, W.},
  title     = {The New Immunosuppressant Leflunomide Appears To Be a Potent Inhibitor of Humoral Xeno Sensitization},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45727},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Immunbiologie},
  language  = {en}
}
@inproceedings{UlrichsEulerMuellerRuchholtz1991,
  author    = {Ulrichs, Karin and Euler, HH and M{\"u}ller-Ruchholtz, W.},
  title     = {A Clinically Successful Protocol to Suppress Autoantibody Production in SLE Patients Is Analyzed For Its Efficacy To Inhibit Natural Xenophile Antibodies (NXA)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45693},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{BaertschLutzSchlatter1991,
  author    = {Baertsch, A. and Lutz, Werner K. and Schlatter, C.},
  title     = {Effect of inhalation exposure regimen on DNA binding potency of 1,2-dichloroethane in the rat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60743},
  year      = {1991},
  abstract  = {1 ,2-Dichloroethane (DCE) was reported to be carcinogenic in rats in a long-tenn bioassay using gavage in com oil (24 and 48 mg/kg/day), but not by inhalation (up to 150-250 ppm, 7 h/day, 5 days/week). The daily dose metabolized was similar in the two experiments. In order to address this discrepancy, the genotoxicity of DCE was investigated in vivo under different exposure conditions. Fernale F-344 rats (183-188 g) were exposed to [1,2-14C]DCE in a closed inhalation chamber to either a low, constant concentration (0.3 mg/l = 80 ppm for 4 h) or to a peak concentration (up to 18 mg/1 = 4400 ppm) for a few minutes. After 12 h in the chamber, the dose metabolized under the two conditions was 34 mg/kg and 140 mg/k:g. DNA was isolated from liver and lung and was purified to constant specific radioactivity. DNA was enzymaticaBy hydrolyzed to the 3' -nucleotides which were separated by reverse phase HPLC. Most radioactivity eluted without detectable or with little optical density' indicating that the major part of the DNA radioactivity was due to covalent binding of the test compound. The Ievel of DNA adducts was expressed in the dose-nonnalized units ofthe Covalent Binding Index, CBI = f.Lmol adduct per mol DNA nucleotide/ mmol DCE per kg body wt. In liver DNA, the different exposure regimens resulted in markedly different CBI values of 1.8 and 69, for "constant-low" and ''peak" DCE exposure Ievels. In the Jung, the respective values were 0.9 and 31. It is concluded that the DNA darnage by DCE depends upon the concentration-time profile and that the carcinogenic potency determined in the gavage study should not be used for low-Ievel inhalation exposure.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{OhgakiLudekeMeieretal.1991,
  author    = {Ohgaki, H. and Ludeke, B. I. and Meier, I. and Kleihues, P. and Lutz, Werner K. and Schlatter, C.},
  title     = {DNA methylation in the digestive tract of F344 rats during chronic exposure to N-methyl-N-nitrosourea},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60759},
  year      = {1991},
  abstract  = {The formation of \(O^6\)-methyldeoxyguanosine (\(O^6\)-MedGuo) was determined by an immuno-slot-blot assay in DNA of various tissues of F344 rats exposed to N-methyl-N-nitrosourea (MNU) in the drinking waterat 400 ppm for 2 weeks. Although the pyloric region of the glandular stomach is a target organ under these experimental conditions, the extent of DNA methylation was highest in the forestomach (185 \(\mu\)mol \(O^6\)-MedGuojmol guanine). Fundus (91 J.!moljmol guanine) and pylorus (105 J.!moljmol guanine) of the glandular stomach, oesophagus (124 \(\mu\)mol/mol guanine) and duodenum (109 )lmoljmol guanine) showed lower Ievels of \(O^6\) - MedGuo but differed little between each other. Thus, no correlation was observed between target organ specificity and the extent of DNA methylation. This is in contrast to the gastric carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), which preferentially alkylates DNA of the pylorus, the main site of induction of gastric carcinomas by this chemical. In contrast to MNU, the nonenzymic decomposition of MNNG is accelerated by thiol compounds (reduced glutathione, L-cysteine), which are present at much higher concentrations in the glandular stomach than in the forestomach and oesophagus. During chronic exposure to MNNG (80 ppm), mucosal cells immunoreactive to 0 6-MedGuo are limited to the luminal surface [Kobori et al. (1988) Carcinogenesis 9:2271-2274]. Although MNU (400 ppm) produced similar Ievels of \(O^6\)-MedGuo in the pylorus, no cells containing methylpurines were detectable by immunohistochemistry, suggesting a more uniform methylation of mucosal cells by MNU than by MNNG. After a single oral dose of MNU (90 mg/kg) cells containing methylpurines were unequivocally identified using antibodies to \(O^6\)-MedGuo and the imidazole-ring-opened product of 7-methyldeoxyguanosine. In the gastric fundus, their distribution was similar to those methylated by exposure to MNNG, whereas the pyloric region contained immunoreactive cells also in the deeper mucosallayers. After a 2-week MNU treatment, the rate of cell proliferation, as determined by bromodeoxyuridine immunoreactivity, was only slightly enhanced in the oesophagus andin the fundus, but markedly in the forestomach and the pyloric region of the glandular stomach. lt is concluded that the overall extent of DNA methylation, the distribution of alkylated cells within the mucosa and the proliferative response all contribute to the organ-specific carcinogenicity of MNU.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{Lutz1991,
  author    = {Lutz, Werner K.},
  title     = {Dose-response relationship for chemical carcinogenesis by genotoxic agents},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60766},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Toxikologie},
  language  = {en}
}
@article{MollMuellerGillitzeretal.1991,
  author    = {Moll, Heidrun and M{\"u}ller, Christoph and Gillitzer, Reinhard and Fuchs, Harald and R{\"o}llinghoff, Martin and Simon, Markus M. and Kramer, Michael D.},
  title     = {Expression of T-cell-associated serine proteinase-1 during murine Leishmania major infection correlates with susceptibility to disease},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61311},
  year      = {1991},
  abstract  = {The expression of T-cell-associated serine proteinase 1 (MTSP-1) in vivo during Leishmania major infection was analyzed in genetically resistant C57BL/6 mice and in genetically susceptible BALB/c mice. Using a monoclonal antibody as well as an RNA probe specific for MTSP-1 to stain tissue sections, we found T cells expressing MTSP-1 in skin lesions and spleens of mice of both strains. In skin lesions, MTSP-1-positive T cells could be detected as early as 3 days after infection. Most importantly, the frequency of T cells expressing MTSP-1 was significantly higher in susceptible BALB/c mice than in resistant C57BL/6 mice. These findings suggest that MTSP-1 is associated with disease-promoting T cells and that it may be an effector molecule involved in the pathogenesis of cutaneous leishmaniasis.},
  subject      = {Biologie},
  language  = {en}
}
@article{FeifelRodriguesdeMirandaStrohmannetal.1991,
  author    = {Feifel, R. and Rodrigues de Miranda, J. F. and Strohmann, C. and Tacke, Reinhold and Aasen, A. J. and Mutschler, E. and Lambrecht, G.},
  title     = {Selective labelling of muscarinic M\(_1\) receptors in calf superior cervical ganglia by [\(^3\)H](\(\pm\))-telenzepine},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64082},
  year      = {1991},
  abstract  = {A method was developed to detennine the affinities of antimuscarinic drugs at M\(_1\) receptors. [\(^3\)H](±)-Telenzepine served as radioligand in crude preparations of calf superior cervical ganglia and showed high affinity for a single receptor population. consisting of M1 receptors (K\(_D\) = 1.12 nM). Kinetic experiments showed monophasic association (k\(_1\) =0.017 min\(^{-1}\) nM\(^{-1}\) ) and dissociation (k\(_1\) = 0.017 min\(^{-1}\) ) kinetics, the half-life of dissociation being 41 min at 37°C. The kinetie K\(_D\) value amounted to 1.00 nM. M\(_1\) affinities for pirenzepine, methoctramine. hexahydro-sila-difenidol and p-fluoro-hexahydro-sila-difenidol detennined in competition experiments were similar to those found in functional studies with MI receptors in rabbit isolated vas deferens. The binding assay was used to deterriline the affinities of the (R) and (S) enantiomers of tertiary (trihexyphenidyl, hexahydro-difenidol. hexbutinol, p-fluoro-hexbutinol) and quatemary musearlnie antagonists (trihexyphenidyl methiodide. hexbutinol methiodide). Comparison of results obtained with the rabbit vas deferens suggested that the ionic environment may influence the affinities.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{StrohmannTackeMatternetal.1991,
  author    = {Strohmann, C. and Tacke, Reinhold and Mattern, G. and Kuhs, W. F.},
  title     = {Bis(2,3-naphthalindiolato)[2-(pyrrolidinio)ethyl]silicat: Synthese und strukturelle Charakterisierung eines zwitterionischen \(\lambda_5\)-Spirosilicates},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64095},
  year      = {1991},
  abstract  = {The zwi tterionic spirocyclic bis(2,3-naphthalenediolato )[2-(pyrrolidinio )ethyl]silicate [ ( C\(_{10}\)H\(_6\)O\(_2\)-SiCH\(_2\)CH\(_2\)(H)NC\(_4\)H\(_8\), 3 was synthesized and its structure characterized (single crystal X-ray structural analysis; \(^1\)H, \(^{13}\)C and \(^{29}\)Si NMR studies of solutions in DMSO). 3 was obtained by reaction of cyclohexylmethoxyphenyl(2-pyrrolidinoethyl)silane [C\(_6\)H\(_{11}\)(CH\(_3\)O)Si(C\(_6\)H\(_5\))CH\(_2\)CH\(_2\) NC\(_4\)H\(_8\), 4] with 2,3-dihydroxynaphthalene [C\(_{10}\)H\(_6\)(OH)\(_2\)] in acetonitrile at room temperature (isolated as 3·CH\(_3\)CN, yield 81 \%). The formation of 3 involves two unusual Si-C cleavage reactions (cleavage of Si-C\(_6\)H\(_5\) and Si-C\(_6\)H\(_{11}\) under mild reaction conditions). In addition, 3 was prepared by reaction of 2,3-dihydroxynaphthalene with dimethoxyphenyl(2-pyrrolidinoethyl)silane [C\(_6\)H\(_5\) (CH\(_3\)O)\(_2\)SiCH\(_2\)CH\(_2\)NC\(_4\)H\(_8\) , 5] and trimethoxy( 2-pyrrolidinoethyl)silane [(CH\(_3\)O)\(_3\)SiCH\(_2\)CH\(_2\)NC\(_4\)H\(_8\), 6], respectively (isolated as 3·CH\(_3\)CN; yields 83 and 86\%, respectively). 3·CH\(_3\)CN crystallizes in the space group Pbca with a = 8.877(2) A. b-= 22.823(4) {\"A}, c""" 24.597(4) A, and Z = 8 (R- 0.0592, Rw = 0.0529). The pentacoordinated Si atom of 3·CH\(_3\)CN is surrounded by its ligands in a nearly ideal square-pyramidal fashion (four basal 0 atoms and one apical C atom). The CH3CN molecule does not coordinate to the Si atom.},
  subject      = {Anorganische Chemie},
  language  = {de}
}
@article{TackeBrakmannWuttkeetal.1991,
  author    = {Tacke, Reinhold and Brakmann, S. and Wuttke, F. and Fooladi, J. and Syldatk, C. and Schomburg, D.},
  title     = {Stereoselective microbial reduction of racemic acetyl(t-butyl)methylphenylsilane by Trigonopsis variabilis (DSM 70714) and Corynebacterium dioxydans (ATCC 21766)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64109},
  year      = {1991},
  abstract  = {(SiR,CR)- and (SiS,CR)-t-butyl(l-hydroxyethyl)methylphenylsilane [(SiR,CR)-2 and (SiS,CR)-3] have been prepared by (R)-selective microbial rcduction of racemic acetyl(t-butyl)methylphenylsilane (rac-1) using resting free cells of the yeast Trigonopsis variabilis (DSM 70714) or the bacterium Corynebacterium dioxydans (ATCC 21766). The biotransfonnations were carried out on a 10 g scale. Afterseparation by column chromatography on silica gel, the optically active diastereomers (SiR,CR)-2 and (SiS,CR)-3 produccd by T. variabilis were obtained in good yields [74\% ((SiR,CR)-2). 78\% ((SiS,CR)-3)]. The products obtained from the reduction with C. dioxydans were isolated in significantly lower yields [20\% ((SiR,CR)-2), 20\% ((SiS,CR)-3)]; reaction conditions not optimized). Both bioconversions gave products with high enantiomeric purities (T. variabilis: 91\% ee ((SiR,CR)-2), 96\% ee ((SiS,CR)-3); C. dioxydons: ~ 991 ee ((SiR,CR)-l), ~ 99\% ee ((SiS,CR)-3)). To throw light on the stereochemical aspects of these biotransfonnations, an X-ray diffraction study was carried out on the 3,5-dinitrobenzoate of rac-(SiR,CS/SiS,CR)-3. In addition, 1H NMR spectroscopic stereochemical correlation studies were performed with the (S)-MTPA esters derived from (SiR,CR)-l, (SiS,CR)-3, rac-(SiR,CRjSiS,CS)-2 and rac-(SiR,CSjSiS,CR)-3 [rac-(SiR,CR/ SiS,CS)-2 and rac-(SiR,CS/SiS,CR)-3 were obtained by reduction of rac-1 with LiAIH\(_4\) in diethylether, followed by chromatographic separation of the diastereomers on silica gel]. These stereochemical studies allowed assignment of the absolute configurations and enantiomeric purities of the biotransformation products.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{LehrnbecherMerzSebaldetal.1991,
  author    = {Lehrnbecher, T. and Merz, H. and Sebald, Walter and Poot, M.},
  title     = {Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: no synergism between interleukin 2 and interleukin 4},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62491},
  year      = {1991},
  abstract  = {Cell kinetic studies of T cells stimulated with the interleukin 2 (11-2), D-4, or both lymphokines were performed with conventional [3H] thymidine incorporation and with the bivariate BrdU/Hoechst technique. 11-2 and 11-4 are able to drive phytohemagglutininactivated T cells through more than one cell cycle. Neither synergistic nor inhibitory efl'ect on T -cell proliferationwas seen for the stimulation with both 11-2 and 11-4 as compared with the effect ofll-2 alone. The quantitative data ofthe cell cycle distribution ofphytohemagglutininactivated T cells suggestthat the population ofll-4-responsive cells is at least an overlapping population, if not a real subset of the ·population of the 11-2-responsive cells.},
  subject      = {Biochemie},
  language  = {en}
}
@article{MerzFliednerOrschescheketal.1991,
  author    = {Merz, H. and Fliedner, A. and Orscheschek, K. and Binder, T. and Sebald, Walter and M{\"u}ller-Hermelink, H. K. and Feller, A. C.},
  title     = {Cytokine expression in T-cell lymphomas and Hodgkin's disease. Its possible implication in autocrine or paracrine production as a potential basis for neoplastic growth},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62483},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Biochemie},
  language  = {en}
}
@article{KruseLehrnbecherSebald1991,
  author    = {Kruse, N. and Lehrnbecher, T. and Sebald, Walter},
  title     = {Site-directed mutagenesis reveals the importance of disulfide bridges and aromatic residues for structure and proliferative activity of human interleukin-4},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62505},
  year      = {1991},
  abstract  = {Mutant proteins (muteins) of human lnterleukin-4 (llA) were constructed by means of in vitro mutagenesis. The muteins were expressed in E. co/1, submitted to a renaturation and purification protocol and analysed for biological activity. Exchange of the cysteines at either position 46 or 99 which form one of the three disulfide bridges resulted. in a nearly co•mplete loss · of biological actiyity and an unstable protein. The exchange of tyrosine 124 also inactivated the protein, while a mutation of tyrosine 56 left some residual activity. Exchange of the other four cysteines or of · the single tryptophane had smaller etTects.},
  subject      = {Biochemie},
  language  = {en}
}
@article{PreiserSchmartzVanderLindenetal.1991,
  author    = {Preiser, J. C. and Schmartz, D. and Van der Linden, P. and Content, J. and Vanden Bussche, P. and Buurman, W. and Sebald, Werner and Dupont, E. and Pinsky, M. R. and Vincent, J. L.},
  title     = {Interleukin-6 administration has no acute hemodynamic or hematologic effect in the dog},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62511},
  year      = {1991},
  abstract  = {To investigate the possible hemodynamic efl'ects of interleukin-6 (IL-6), a single dose of 15 mcg/kg of recombinant IL-6 isolated from Escherichia coli was injected intravenously in six pentobarbital-anesthetized dogs. After 30 min, saline infusion was performed to maintain the - pulmonary artery balloon-occluded pressure at baseline Ievel. The animals were observed for up to 5 hours. No other hemodynamic alteration was observed than a gradual decline in cardiac output attributed to anesthesia. Hematologic variables, blood glucose, and total serum proteins were also constant. IL-6 levels were markedly elevated in the blood, bot no tumor necrosis factor activity was detected. Thus a primary role for IL-6 in the early cardiovascular alterations associated with septic shock seems unlikely.},
  subject      = {Biochemie},
  language  = {en}
}
@article{TonyLehrnbecherMerzetal.1991,
  author    = {Tony, H. P. and Lehrnbecher, T. and Merz, H. and Sebald, Werner and Wilhelm, M.},
  title     = {Regulation of IL-4 responsiveness in lymphoma B cells},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62520},
  year      = {1991},
  abstract  = {The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity.},
  subject      = {Biochemie},
  language  = {en}
}
@article{KlotzVogtTawfikSchlieper1991,
  author    = {Klotz, Karl-Norbert and Vogt, H. and Tawfik-Schlieper, H.},
  title     = {Comparison of A\(_1\) adenosine receptors in brain from different species by radioligand binding and photoaffinity labelling},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60388},
  year      = {1991},
  abstract  = {Radioligand binding to A\(_1\) adenosine receptors at brain membranes from seven species was investigated. The antagonist 8-cyclopentyl-1 ,3-[\(^3\)H]dipropylxanthine ([\(^3\)H]DPCPX) bound with affinities between 0.17 nM in sheep brain and 2.1 nM in guinea pig brain. Competition of several antagonists for [\(^3\)H]DPCPX binding showed that the most potent compounds were DPCPX with K\(_i\) values of 0.05 nM in bovine brain and 1.1 nM in guinea pig brain and xanthine amine congener (XAC) with K\(_i\) values of 0.03 nM in bovine brain and 5.5 nM in guinea pig brain. The differences in affinity of the agonist radio Iigand 2-chloro-N\(^6\) -[\(^3\)H]cyclopen tyladenosine ([\(^3\)H]CCP A) were less pronounced, rauging from a K\(_D\) value of 0.12 nM (hamster brain) to 0.42 nM (guinea pig brain). Agonist competition for [\(^3\)H]DPCPX binding of photoaffinity labelling, however, exhibited marked species differences. N-Ethylcarboxamidoadenosine (NECA) and S-N\(^6\)-phenylisopropyladenosine (S-PIA) showed 20 to 25-fold different K\(_D\) values in different species. NECA had a particularly high affinity in guinea pig brain and was only two-fold less potent than R-PIA. Thus, the difference from the "classical" A\(_1\) receptor profile (R-PIA > -NECA > S-PIA) is not sufficient to speculate that A\(_1\) receptor subtypes may exist that are coupled to different effector systems. Our data show that these difference can easily be explained by species differences.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{vanCalkerSteberKlotzetal.1991,
  author    = {van Calker, D. and Steber, R. and Klotz, Karl-Norbert and Greil, W.},
  title     = {Carbamazepine distinguishes between adenosine receptors that mediate different second messenger responses},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60392},
  year      = {1991},
  abstract  = {The mechanism of the therapeutic and prophylactic effects of carbamazepine (CBZ) in affective psychoses is unknown but may in part be related to the potent competitive interaction of CBZ with adenosine-binding sites in the brain. The antioonvulsant and sedative properties of CBZ are reminiscent of the effects evoked by adenosine-agonists and contrast sharply with the opposite aclions of adenosine-antagonists like caffeine. However. indirect evidence suggests an antagonist- rather than an agonist-like activity of CBZ at adenosi11e-receptors. We have used various model systems, in which adenosine receptor subtypes mediate different second messenger-responses, to investigate this apparent paradox. CBZ was found to antagonize the A\(_1\) receptor-mediated inhibition of cydic AMP accumulation in cultured astroblasts and in GH3-cells. Furthermore, CBZ also inhibits the adenosine-induced increase in the level of cyclic AMP in cultured astroblasts, which is mediated by low-affinity A\(_{2b}\)-receptors. ln contrast, CBZ does not block the inhibition elicited by adenosine-agonists of the agonist-induced increased formation of inositolphosphates in human neutrophils, which is mediated by high-affinity A\(_{2a}\)-receptors. The specific antagonism by CBZ of A\(_1\)- but not of high-affinity A\(_{2a}\)-receptors was further supported by binding experiments using rat brain membranes. These results suggest tbat the paradox of CBZ's antagonistic effects at adenosine-receptors might be at least partially reconciled by a selective antagonistic action of CBZ at A\(_1\)recertors but not at high-affinity A\(_{2a}\)-receptors.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{OttBenderBlumetal.1991,
  author    = {Ott, M. and Bender, L. and Blum, G. and Schmittroth, M. and Achtmann, M. and Tsch{\"a}pe, H. and Hacker, J{\"o}rg},
  title     = {Virulence patterns and long range mapping of extraintestinal Escherichia coli K1, K5 and K100 isolates: Use of pulse field gel electrophoresis},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59738},
  year      = {1991},
  abstract  = {A total of 127 extraintestinal Escherichia coli strains of the capsule serotypes Kl, KS, and KlOO from human and animal sources were analyzed for DNA sequences specific for the genes for various adhesins (P fimbriae fpap] and P-related sequences fprs], S fimbriae [s/a)/FlC fimbriae [foc], and type I fimbriae lfim]), aerobactin (aer), and hemolysin (hly). The expression of corresponding virulence factors was also tested. Twenty-four selected strains were analyzed by long-range DNA mapping to evaluate their genetic relationships. DNA sequences for the adhesins were often found in strains not expressing them, while strains with hemolysin and aerobactin genes usually did express them. Different isolates of the same serotype orten expressed different virulence patterns. The use of virulence-associated gene probes for Southern hybridization with genomic DNA fragments separated by pulsed-field gel electrophoresis revealed that a highly heterogeneous restriction fragment length and hybridization pattern existed even within strains of the same serotype. Long-range DNA mapping is therefore useful for the evaluation of genetic relatedness among individual isolates and facilitates the performance of .precise molecular epidemiology.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{BenderOttDebesetal.1991,
  author    = {Bender, L. and Ott, M. and Debes, A. and Rdest, U. and Heesemann, J. and Hacker, J{\"o}rg},
  title     = {Distribution, expression and long range mapping of legiolysin gene (lly) specific DNA sequences in Legionellae},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59744},
  year      = {1991},
  abstract  = {The legiolysin gene (lly) cloned from Legionella pneumophila Philadelphia 1 confers the phenotypes of hemolysis and browning of the culture medium. An internal Uy-specific DNA probe was used in Southern hybridizations for the detection of Uy-specific DNA in the genomes of legioneUae and other gram-negative pathogenic bacteria. Under conditi9ns of high stringency, tlie Uy DNA probe specifically reacted with DNA fragments fr9m L. pneumophi{\"u}z isolates; by reducing stringency, hybridization was also observed for all other Legionella strains tested. No hybridization occurred with DNAs isolated from bact~ria of other genera. The Uy genewas mapped by pulsed-field gel electrophoresis to the respective genomic Notl fragments of Legionelltz isolates. By using antilegiolysin monospecific polyclonal antibodies in Western blots (immunoblots), Lly proteins could be detected only in L. pneumophila isolates.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{OttMessnerHeesemannetal.1991,
  author    = {Ott, M. and Messner, P. and Heesemann, J. and Marre, R. and Hacker, J{\"o}rg},
  title     = {Temperature dependent expression of flagella in Legionella},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59755},
  year      = {1991},
  abstract  = {Legionel/a pneumophila, the causative agent of Legionnaires' disease, was analysed by electron microscopy for production of surface structures. Crystalline surface (S-) layers and fimbriae were not detected, but monotrichous flagellation was seen. Polyclonal antibodies specific for the 47 kDa ftagellin subunit of L. pneumophila Philadelphia I were used in Western blots to confirm the presence of flagella subunits in various L. pneumophila strains tested, but the antiserumalso reacted with flagellin subunits of L. micdlulei, L. hackelia (serogroup (SG) l and SG21 and L./ongbetichae (SG2). Flagellation of Legionellae was shown to be temperature regulated. When the growth temperature of virulent and avirulent variants of strain L. pneumophila Philadelphia I was shifted from 30 oc to either 37 or 41 oc, a decrease in the percentage offtagellated bacteria within the populationwas observed.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{OttBenderChirinosetal.1991,
  author    = {Ott, M. and Bender, L. and Chirinos, E. and Ehret, W. and Hacker, J{\"o}rg},
  title     = {Phenotype versus genotype of the 19 kd peptido-glycan associated protein of Legionella (PplA) among Legionellae and other gram-negative bacteria},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59768},
  year      = {1991},
  abstract  = {The protein PpiA (19 kD) cloned from a genomic library of Legionella pneumophila, Philadelphia 1, represents a peptido-glycan associated outer membrane protein in recombinant E. coli K-12 and L. pneumophila. lt exhibits distinct sequence homology to Iipoproteins of Haemophilus influenzae and E. coli. A ppiA specific DNA probe generated by PCR was used in Southern hybridizations of chromosomal DNA of Legionella strains and other Gram-negative pathogens. Under conditions of high stringency, hybridization could only be observed in L. pneumophila isolates, but alt other Legionella strains tested displayed hybridization under lower stringency. No signals appeared after hybridization of chromosomal DNA from a variety of other bacteria. Using anti-PpiA monospecific polyclonal antibodies in Western blots, it was demonstrated that PpiA related proteins of nearly the same size are found in all L. pneumophila isolates and in a variety of, but not alt, the Legionella species analysed here.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{FoernzlerWittbrodtSchartl1991,
  author    = {F{\"o}rnzler, Dorothee and Wittbrodt, Joachim and Schartl, M.anfred},
  title     = {Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences in Xiphophorus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61726},
  year      = {1991},
  abstract  = {Melanoma formation in Xiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by the Tu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecu/ar identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes in Xiphophorus, two of which have been previously reported to be linked to locus R. We confinn that ES 1 is a distant marker for R ( approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity Ievels in all organs tested. ES4, which has also been reported to be linked to R, was found to be a misclassification of liver ES1. In an attempt to identify markersthat bridge the large distance between ESl and R, we have generated DNA probes which are highly polymorphic. They will be useful in finding Iandmarks on a physical map of the R-containing chromosomal region.},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{SchartlSchluppSchartletal.1991,
  author    = {Schartl, Manfred and Schlupp, Ingo and Schartl, Angelika and Meyer, Manfred K. and Nanda, Indrajit and Schmid, Michael and Epplen, J{\"o}rg T. and Parzefall, Jakob},
  title     = {On the stability of dispensable constituents of the eukaryotic genome: Stability of coding sequences versus truly hypervariable sequences in a clonal vertebrate, the amazon molly, Poecilia formosa},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61731},
  year      = {1991},
  abstract  = {In dooal unisexual vertebrales, the genes specifying the males become dispensable. To study tbe rate of such geoes the gynogeoetic all-female fisb Poecilillfonnolll was treated with androgens. Phenotypic males were obtained that exbibited the complete set of male cbaracteristics of dosely related gooocboristic species, induding body proportions, pigmentation, the extremely complex insemination apparatus of poecil{\"u}d fish, sexual bebavior, and spermatogeoesls. Tbe apparent stabllity of such genic structures, induding those involved in androgen regulation, is contrasted by high instability of noncoding sequeaces. Frequent mutations, thelr donal transmission, and at least two truly hypervariable Iod leading to individual difl'ereaces between these othenrise donal organisms were detected by DNA fingerprinting. These observations substantiate the concept that also in "ameiotic" vertebrates certain compartments of the genome are more prooe to mutatiooal alterations than others.},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{WinklerVielkindSchartl1991,
  author    = {Winkler, Christoph and Vielkind, J{\"u}rgen R. and Schartl, Manfred},
  title     = {Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61743},
  year      = {1991},
  abstract  = {Species of small fish are becoming useful tools for studies on vertebrate development. Wehave investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporaland spatial expression patterns ofbacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as weil as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{OttBenderMarreetal.1991,
  author    = {Ott, M. and Bender, L. and Marre, R. and Hacker, J{\"o}rg},
  title     = {Pulsed field electrophoresis of genomic restriction fragments for the detection of nosocomial Legionella pneumophila in hospital water supplies},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59672},
  year      = {1991},
  abstract  = {Ten Legionella pneumophUa strains isolated from dift'erent sources were analyzed according to their restriction fragment patterils obtained by cle~vage of gen.omic DNA With Notl and Sftl and separation by pulsed field electrophoresis. Three L. pneumophila isolate~ from a nosocomial outbreak in L{\"u}~k (Germany) and three other L. prreumophilll stralns independently isolated from a water tap located in the care unit where tbe patients were bospitalized 'xhibited identical restricti9n fragment profiles. Therefore, we concluded that these environment81 spee~ens were the source of the Legionnatres dlsease. Anotber two isolates from patients and two strains from the environment, all unrelated to the outJlreak described, sbowed different cleavage patterns.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{HackerOttLudwigetal.1991,
  author    = {Hacker, J{\"o}rg and Ott, M. and Ludwig, B. and Rdest, U.},
  title     = {Intracellular survival and expression of virulence determinants of Legionella pneumophila},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59681},
  year      = {1991},
  abstract  = {Legionella pneumophila, the causative agent of Legionnaires' disease is able to live and multiply within macrophages as weil as within protozoan organisms. Legionella strains inhibit phagosome-lysosome fusion and phagosome acidification. By using two different cell culture systems, one derived from human macrophages and the other from human.embryo lung fibro:blastic cells, it is demonstrated that Legionella strains lose their virulence following cultivation in the laboratory. In order to study the mechanisms involved in intracellular survival of Legionella a genomic library of strain Legionella pneumophila Philadelphia I was established in Escherichia coli K-12. By cosmid cloning technique we were able to clone five putative virulence factors, two of which exhibit hemolytic activities and three of which represent membrane-associated proteins of 19, 26 and 60 kilodalton. One of the hemolytic proteins, termed legiolysin, represents a new toxin which specifically lyses human erythrocytes. The other hemolysin exhibits proteolytic properties in addition and is cytolytic for Vero and CHO cells. Further sturlies will be necessary to determine the exact role of the cloned proteins in the pathogenesis of Legionella. Zusammenfassung: Intrazellul{\"a}res {\"U}berleben},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{OttHacker1991,
  author    = {Ott, M. and Hacker, J{\"o}rg},
  title     = {Analysis of the variability of S fimbriae expression in an Escherichia coli pathogen.},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59695},
  year      = {1991},
  abstract  = {The uropathogenic Escherichia coli wiJd..:type strain 536 produces S-fimbriae, P-related fimbriae and type I fimbriae. Using immuno-colony dot and ELISA techniques, variants were detected showing an increased degree of S-fimbrial production. It was demonstrated by itrtmunofluorescence microscopy that in noimal (wild-type) and hyperS- fimbriated E. coli populaiions non-fimbriated cells also · exist, and that the percentage of Sfinibrlated and non-fimbriated bacteria was roughly identica1 in either population. Hyper-Sfimbriated variants could be stably maintained. The transition from wild-type to hyper-S-fimbriation, which occurs spontaneously, is markedly higher than vice versa. Southern blot analysis of the S fimbrial adhesin (sfa) determinants of normal and hyper-fimbriated strains revealed no marked difference in the gene structure.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{WintermeyerRdestLudwigetal.1991,
  author    = {Wintermeyer, E. and Rdest, U. and Ludwig, B. and Debes, A. and Hacker, J{\"o}rg},
  title     = {Characterization of legiolysin (lly); responsible for hemolytic activity, colour production and fluorescence of Legionella pneumophila},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59706},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{BlumOttCrossetal.1991,
  author    = {Blum, G. and Ott, M. and Cross, A. and Hacker, J{\"o}rg},
  title     = {Virulence determinants of Escherichia coli O6 extraintestinal isolates analysed by Southern hybridizations and DNA long range mapping techniques},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59717},
  year      = {1991},
  abstract  = {A total of 16 Escherichia coli 06 strains isolated from cases of extraintestinal infections were analysed for the genetic presence and phenotypic expression of fimbrial adhesins ( P, S/FIC, type I), aerobactin and hemolysin. ln addition restriction fragment length polymorphisms (RFLPs) of Xbal-cleaved genomic DNA of seven selected strains, separated by orthogonal field alternation gel electrophoresis {OFAGE) were determined and virulence-associated DNA probes were used for Southern hybridization studies of the Xbal-cleaved genomic DNAs. The virulence characteristics and hybridization patterns obtained differed between the various isolates. ln three isolates hemolysin genes and P fimbrial determinants were located on the same Xbal fragments. Furthermore, multiple copies of FIC determinants (foc) could be detected in two strains. Our data show that the new technique of pulse field electrophoresis tagether with Southern hybridization represents a powerful tool for the genetic analysis of pathogenic bacteria.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{LudwigSchmidMarreetal.1991,
  author    = {Ludwig, B. and Schmid, A. and Marre, R. and Hacker, J{\"o}rg},
  title     = {Cloning, genetic analysis and nucleotide sequence of a determinant coding for a 19 kd peptidoglycan-associated protein (Ppl) of Legionella pneumophila},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59721},
  year      = {1991},
  abstract  = {A genomic library of Legionello pneumophihz, the causative agent of Legionnaires disease in humans, was constructed in Escherichill coli K-12, and the recombinant clones were screened by immuno-colony blots with im antiserum raised against heat-killed L. pneumophilo. Twenty-three clones coding for a LegioneUa-specific protein of 19 kDa were isolated. The 19-kDa protein, which represents an outer membrane protein, was found tobe associated with the peptidoglycan layer bothin L. pneumophilo andin the recombinant E. coli clones. This was shown by electrophoresis and Western immunoblot analysis of bacterial cell membrane fractions witb a monospecific polyclonal 19-kDa protein-specific antiserum. Tbe protein was termed peptidoglycan-associated protein of L. pneumophilo (Ppl). The corresponding genetic determinant, ppl, was subcloned on a 1.8-kb Clol fragment. DNA sequence studies revealed that two open reading frames, pplA and pplB, coding for putative proteins of 18~9 and 16.8 kDa, respectively, were located on the Clol fragment. Exonuclease 111 digestion studies confirmed tbat pplA is the gene coding for the peptidoglycan.;.associated 19-kDa protein of L. pneumophilo. The amino acid sequence of PpiA exhibits a high degree of homology to the sequences of the Pal Iipoproteins of E. coli K-12 and liaemophilus injluenvze.},
  subject      = {Infektionsbiologie},
  language  = {en}
}
@article{BuendgenEngelsPeyerimhoff1991,
  author    = {B{\"u}ndgen, P. and Engels, Bernd and Peyerimhoff, S.D.},
  title     = {An MRD-CI study of low-lying electronic states in CaF},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58880},
  year      = {1991},
  abstract  = {Dipole moments and various spectroscopic constants of some low-lying electronic states of the CaF molecule have been calculated using the multireference single· and double-excitation configuration-interaction (MRD-CI) method. The electronic structure of the highly ionic molecule in various excited states can be explained in tenns of different polarisations of the mainly Cacentered valence electron in the field of the F\(^-\) anion. Plots of natural orbitals occupied by the valence electron in the different states give a qualitative picture of the charge distribution and provide a visualisation of the different polarisations of the valence electron in the various states. Comparisons with the electrostatic polarisation model ofT{\"o}rring, Ernstand K{\"a}ndler (TEK model) are made. The unknown A' \(^2 \Delta\) state is predicted to lie about 21200 cm\(^{-1}\) above the ground state.},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{Engels1991,
  author    = {Engels, Bernd},
  title     = {Estimation of the influence of the configurations neglected within truncated MR-CI wavefunctions on molecular properties},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58890},
  year      = {1991},
  abstract  = {Reliable prediction of the isotropic hyperfine coupling constant, a\(_{iso}\), is still a difficult task for ab initio calculations. Strong dependence on the method used for its calculation is found. Within a truncated multi-referencc ansatz a\(_{iso}\) is strongly affected by the size ofthe reference space and the nurober of terms in the truncated Cl expansion. In the present paperdifferent effects of the neglected Cl space are discussed. Modified B\(_K\) and A\(_K\) methods are used to estimate the contributions ofthe neglected configurations. lt can be shown that a combination of both methods is able to recover about 90-9 S\% of the total error in a\(_{iso}\)· Furthermore, it was found that to obtain about 90\% of the B\(_K\) correction only about I 0-20\% ofthe configurations within H0 have to be corrected.},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{PericEngelsPeyerimhoff1991,
  author    = {Peric, M. and Engels, Bernd and Peyerimhoff, S.D.},
  title     = {Ab initio investigation of the vibronic structure of the C\(_2\)H spectrum Calculation of the hyperfine coupling constants for the three lowest lying electronic states},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58901},
  year      = {1991},
  abstract  = {The hyperfine coupling constants (isotropic hfcc and four Cartesian components of the ani~ tropic tensor) are calculated for all three atoms of C\(_2\)H in its three lowest-lying electronic states at various molecu)ar geometries by means of the ab initio configuration interaction ( MRO.CI) method. The off-diagonal electronic matrix elements involving the two species ofthe A' symmetry are also computed. A diabatic transforrnation is perforrned Jeading to simple geometrical depen· dences of the hyperline coupling constants.},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{PericEngelsPeyerimhoff1991,
  author    = {Peric, M. and Engels, Bernd and Peyerimhoff, S.D.},
  title     = {Ab initio investigation of the vibronic structure of the C\(_2\)H spectrum Computation of the vibronically-averaged values for the Hyperfine Coupling Constants},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58915},
  year      = {1991},
  abstract  = {The vibronically averaged values for tbe hyperfine coupling constants in the X\(^2 \sum\)-A\(^2 \Pi\) system of the ethynyl radical are computed by means of tbe ab initio metbod calculations. The results point at tbe importance of taking into account the coupling of a1l tbree electronic states in question ( I\(^2\)A', 2\(^2\)A', and 1\(^2\)A") for a reliable explanation of the available experimental findings. The mean values of the hfcc's for K = 0 and 1 levels in \(^{13}\)C\(_2\)H and \(^{13}\)C\(_2\)D in the energy range up to 6000 cm\(^{-1}\) are predicted.},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{MaurerSerflingterMeulenetal.1991,
  author    = {Maurer, Bernd and Serfling, Edgar and ter Meulen, Volker and Rethwilm, Axel},
  title     = {Transcription factor AP-1 modulates the activity of the human foamy virus long terminal repeat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61444},
  year      = {1991},
  abstract  = {The human foamy virus (HFV) contains within the UJ region of its long terminal repeat (L TR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as weil as bacterially expressed Jun and Fos proteins bind to these AP-1 sites. By conducting transient expression assays using chloramphenicol acetyltransferase plasmids carrying LTR sequences with point-mutated AP-1 sites it was found that the three AP-1 sites contribute to the optimal activity ofthe HFV promoter. It is shown that lnduction of the HFV L TR by 12-O-tetradecanoylphorbol-13-acetate (TPA) and serum factors is mediated through the AP-1 sites.},
  subject      = {Virologie},
  language  = {en}
}
@article{BotheAguzziLassmannetal.1991,
  author    = {Bothe, Katrin and Aguzzi, Adriano and Lassmann, Hans and Rethwilm, Axel and Horak, Ivan},
  title     = {Progressive encephalopathy and myopathy in transgenic mice expressing human foamy virus genes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61453},
  year      = {1991},
  abstract  = {Transgenie mice carrying the bel region of human foamy retrovirus (HFV) under transcriptional control of its own long terminal repeat expressed tbe transgene in their centrat nervous systems and in smootb and striated muscle tissues. The animals developed a progressive degenerative disease of tbe centrat nervous system and of the striated muscle. Because expression of tbe transgene was dosely correlated witb the appearance of structural damage and inflammatory reactions were scanty, the disease is likely to be caused directly by tbe HFV proteins. These unexpected findings call for a reevaluation of tbe patbogenic potential of HFV in humans.},
  subject      = {Virologie},
  language  = {en}
}
@article{KauppSchleyerStolletal.1991,
  author    = {Kaupp, Martin and Schleyer, Paul. von Rague and Stoll, Hermann and Preuss, Heinzwerner},
  title     = {The Question of bending of the Alkaline Earth Dihalides MX\(_2\) (M = Be, Mg, Ca, Sr, Ba; X = F, C1, Br, I). An ab Initio Pseudopotential Study},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60166},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{KauppStollPreussetal.1991,
  author    = {Kaupp, Martin and Stoll, Hermann and Preuss, Heinzwerner and Kaim, Wolfgang and Stahl, Thomas and van Koten, Gerard and Wissing, Elmo and Smeets, Wilberth J. J. and Spek, Anthony L.},
  title     = {Theoretical and Experimential Study of Diamagnetic and Paramagnetic Products from Thermal and Light-Induced Alkyl Transfer between Zinc or Magnesium Dialkyls and 1,4-Diaza-1,3-butadiene Substrates},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60173},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{DoerjeWessLambrechtetal.1991,
  author    = {D{\"o}rje, F. and Wess, J. and Lambrecht, G. and Tacke, Reinhold and Mutschler, E. and Brann, M. R.},
  title     = {Antagonist binding profiles of five cloned human muscarinic receptor subtypes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64113},
  year      = {1991},
  abstract  = {A variety of muscarinic antagonists are currently used as tools to pharmacologically subclassify muscarinic receptors into M\(_1\), M\(_2\) and M\(_3\) subtypes. ln the present study I we have determined the affinity proflies of several of these antagonists at five cloned human muscarinic receptors (m1-m5) stably expressed in Chinesehamster ovary cells (CHO-K1). At all five receptorsl the (R)-enantiomers of trihexyphenidyl and hexbutinol displayed considerably higher affinities (up to 525-fold) than their corresponding (S)-isomers. The stereoselectivity ratios [inhibition constant( S)/inhibition constant(R)] for both pairs of enantiomers were lowest at m2 receptors, suggesting that less stringent configurational demands are made by this receptor subtype. The "M\(_1\)-selective" antagonist (R)-trihexyphenidyl displayed high affinities for m1 and m4 receptors. The "M\(_2\)-selective" antagonists himbacinel (±}-5, 11-dihydro-11-1[(2-[(dipropylamino)methyl]-1- piperidinyllethyl)amino]carbonyii-6H-pyrido(213-b)(1 ~4)benzodiazepine- 6-one (AF-DX 384)1 11-(14-[4-(diethylamino)butyl)-1-piperidinyll acetyl)-5~ 11-dihydro-6H-pyrido(2~3-b) (1~4)benzodiazepine-6-one (AQ-RA 741) and (+K11-(12-[(diethylamino)methyl]-1-piperidinyll acetyl)-5~ 11-di-hydro-6H-pyrido(2~3-b)(1,4)benzodiazepine-6-one (AF-OX 250; the (+)-enantiomer of AF-DX 116] exhibited high affinities for m2 and m41 intermediate affinities for m1 and m3 and low affinities for m5 receptors. This selectivity profile was most prominent for AQ-RA 7 41 I which displayed 195- and 129-fold higher affinities for m2 and m4 receptors than for mS receptors. The "M\(_3\)-selective" antagonist (±)-p-fluoro-hexahydro-sila-difenidol hydrochloride (pFHHsiD) exhibited high affinity for m1 I m3 and m4 receptors. 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) bound with up to 7 -fold higher affinities to m1 I m31 m4 and m5 receptors than to m2 receptors. Although none of the tested antagonists showed more than 2-fold selectivity for one subtype over all other subtypes, each receptor displayed a unique antagonist binding profile.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{WaelbroeckCamusTastenoyetal.1991,
  author    = {Waelbroeck, M. and Camus, J. and Tastenoy, M. and Mutschler, E. and Strohmann, C. and Tacke, Reinhold and Lambrecht, G. and Christophe, J.},
  title     = {Binding affinities of hexahydro-difenidol and hexahydro-sila-difenidol analogues at four muscarinic receptor subtypes: constitutional and stereochemical aspects},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64128},
  year      = {1991},
  abstract  = {Hexahydro-sila-difenidoJ and eight analogues behaved as simple cumpetitive inhibitors of eHJN·methyl·scopoJamine binding to homogenates frorn human neuroblastoma NB-OK 1 cells (M\(_1\) sites), rat heart (M\(_2\) sites), rat pancreas (M\(_3\) sites), and rat striatum 'B' sites (M\(_4\) sites). Pyrrolidino- and hexamethyleneimino analogues showed the same sekctivity profile as the parent compound. Hexahydro-sila-difenidol methiodide and the methiodide of p-fluoro-hexahydro·sila-difenidol had a f{\"u}gher affinity but a lower selectivity than the tertiary amines. Compounds containing a p·methoxy, p-chJoro or p-fluoro substituent in the phenyl ring of hexahydro-sila-difenidol showed a qualitative)y similar selectivity profile as the parent compound (i.e., M\(_1\)= M\(_3\) = M\(_4\) >M\(_2\) ), but up to 16-fold lower affinities. o-Methoxy-hexahydro-sila-difenidol has a lower affinity than hexahydro-sila-difeni.:!o! at the four binding sites. lts selectivity profile (M\(_4\) > M\(_1\), M\(_3\) > M\(_2\) ) was different from hexahydro-sila-difenidol. Replacement of the centrat silicon atom of hexahydro-sila-difenidol, p-fluoro-hexahydro-sila-difenidol and thdr quatemary (N-methylated) analogues by a carbon atom did not change their binding affinities significantly. The iour muscarinic receptors showed a higher affinity for the (R)- than for the (S)-enantiomers of hexahydro-difenidol, p-fluorohexahydro-difenidol and their methiodides. The stereoselectivity varied depending on the receptor subtype and drug considered.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{WaelbroeckCamusTastenoyetal.1991,
  author    = {Waelbroeck, M. and Camus, J. and Tastenoy, M. and Mutschler, E. and Strohmann, C. and Tacke, Reinhold and Lambrecht, G. and Christophe, J.},
  title     = {Stereoselectivity of (R)- and (S)-hexahydro-difenidol binding to neuroblastoma M\(_1\), cardiac M\(_2\), pancreatic M\(_3\), and striatum M\(_4\) muscarinic receptors},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64135},
  year      = {1991},
  abstract  = {(R)-Hexahydro-difenidol has a higher affinity for M\(_1\) receptors in NB-OK 1 cells, pancreas M\(_3\) and striatum M\(_4\) receptors (pKi 7.9 to 8.3) than for cardiac M2 receptors (pKi 7 .0). (8)-Hexahydro-difenidol, by contrast, is nonselective (pKi 5.8 to 6.1). Our goal in the present study was to evaluate the importance ofthe hydrophobic phenyl, and cyclohexyl rings of hexahydro-difenidol for the stereoselectivity and reeeptor selectivity of hexahydro-difenidol binding to the four muscarinic receptors. Our results indieated that replacement of the phenyl ring of hexahydro-difenidol by a cyclohexyl group <~ dicyclidol) and ofthe cyclohexyl ring by a phenyl moiety <~ difenidol) indueed a !arge (4- to 80-fold) decrease in binding affinity for all musearlnie receptors. Difenidol had a signifieant preference for M\(_1\) , M\(_3\) , and M\(_4\) over M\(_2\) receptors; dicyclidol, by eontrast, had a greater affinity for M\(_1\) and M\(_4\) than for M\(_2\) and M\(_3\) receptors. The binding free energy deerease due to replacement ofthe phenyl and the cyelohexyl groups of(R)-hexahydro-difenidol by, respectively, a eyclohexyl and a phenyl moiety was almostadditive in the ease of M\(_4\) (striatum) binding sites. In the ease ofthe cardiac M\(_2\), pancreatic M\(_3\) , or NB-OK 1 M\(_1\) receptors the respective binding free energies were not eompletely additive. These results suggest that the four (R)-hexahydro-difenidol ''binding moieties" (phenyl, cyclohexyl, hydroxy, and protonated amino group) cannot simultaneously form optimal interaetions with the M\(_1\), M\(_2\), and M\(_3\) muscarinic receptors. When eaeh of the hydrophobic groups is modified, the position of the whole molecule, relative to the four subsites, was changed to allow an optimal overall interaction with the musearlnie receptor.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{StrohmannBauereckerCammengaetal.1991,
  author    = {Strohmann, C. and Bauerecker, S. and Cammenga, H. K. and Jones, P. G. and Mutschler, E. and Lambrecht, G. and Tacke, Reinhold},
  title     = {Enantiomers of the muscarinic antagonist 1-cyclohexyl-1-(4-fluorophenyl)-4-piperidino-1-butanol (p-fluoro-hexahydro-difenidol): synthesis, absolute configuration, and enantiomeric purity},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64144},
  year      = {1991},
  abstract  = {The enantiomers of the antimuscarinic agent 1-cyclohexyl-1- (4-fluorophenyl)-4-piperidino-1-butanol [(R)- and (S)-p-fluorohexahydro- difenidol] ((R)- and (S)-2a] and their methiodides (R)- 3 and (S)-3 were prepared with high enantiomeric purity. (R)- 2a and (S)-2a (isolated as hydrochlorides) were obtained by catalytic hydrogenation (Pd/C contact) of the corresponding enantiomers of 1-cyclohexyl-1-( 4-fl uorophen yl)-4-piperidino- 2-butyn-1-ol [(R)- and (S)-4]. Reaction of (R)-2a and (S)-2a with rnethyl iodide led to (R)-3 and (S)-3, respectively. The unsaturated precursors (R)- and (S}-4 (enantiorneric purity ~ 99.80 and ~99.94\% e.e.; calorimetric analysis) were prepared by res-sepaolution of rac-4 [available from 4-FC\(_6\)H\(_4\)C(O)C\(_6\)H\(_{11}\) by reaction with LiC ~ CCH\(_2\)NC\(_5\)H\(_{10}\)] using (R)- and (S)-mandelic acid as resolving agents. The absolute configurations of the (R) and (S) enantiomers of 2a, 3, and 4 were determined by an X-ray crystal-structure analysis of (S)-5, the methiodide of (S)-4. (R)- 2a and (R)-3 exhibit a higher affinity for muscarinic M1, M2, M3, and M4 receptors (by up to two orders of magnitude) than their corresponding antipodes (S)-2a and (S)-3, the degree of stereoselectivity depending on the receptor subtype involved. (R)-2a represents a useful tool for rnuscarinic receptor research (affinity profile: M1 ~ M3 ~ M4 > M2).},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{HengelsbergTackeFritscheetal.1991,
  author    = {Hengelsberg, H. and Tacke, Reinhold and Fritsche, K. and Syldatk, C. and Wagner, F.},
  title     = {Synthesis and enantioselective enzymatic hydrolysis of rac-dimethylphenyl[1-(phenylacetamido)- ethyl]silane},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64153},
  year      = {1991},
  abstract  = {Racemic dimethylphenyl(l-(phenylacetamido)ethyl)silane [rac-5) has been made by a four-step synthesis starting from (chloromethyl)dimethylphenylsilane [PhMe\(_2\)SiCH2Cl (1) ~ PhMe\(_2\)SiCH(Cl)Me (rac-2) - PhMe\(_2\)SiCH(l)Me (rac-3) - PhMe2SiCH(NH2)Me (rac-4) ~ PhMe\(_2\)SiCH[N(H)C(O)CH\(_2\)Ph]Me ( rac-5); total yield 41\% ). Enantioselective enzymatic hydrolysis of rac-5, catalyzed by immobilized penicillin G acylase (E.C. 3.5.1.11) from Escherichia coli 5K (pHM 12), gave (R)-(1- aminoethyl)dimethylphenylsilane [( R )-4] in 40\% yield with an enantiomeric purity of 92\% ee.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{TackeMahnerStrohmannetal.1991,
  author    = {Tacke, Reinhold and Mahner, K. and Strohmann, C. and Forth, B. and Mutschler, E. and Friebe, T. and Lambrecht, G.},
  title     = {Cyclohexyl(4-fluorophenyl)(3-piperidinopropyl)silanol (p-fluoro-hexahydro-sila-difenidol, p-F-HHSiD) and derivatives: synthesis and antimuscarinic properties},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64162},
  year      = {1991},
  abstract  = {Four different syntheses of the potent and selective muscanruc antagonist cyclohexyl( 4- fluorophenyl)(3-piperidinopropyl)silanol ( p-fluoro-hexahydro-sila-difenidol, p-F-HHSiD (2b); isolated as hydrochloride 2b· HCl) are described (starting materials: (CH\(_3\)O)\(_2\)SiCH\(_2\)CH\(_2\)CH\(_2\)Cl and Si(OCH\(_3\))\(_4\) ). In addition, the synthesis of the corresponding carbon analogue p-fluoro-hexahydro-difenidol ( p-F-HHD (2a); isolated as 2a· HCI) and the syntheses of three p-F-HHSiD derivatives (3-5), with a modified cyclic amino group, are reported (3: piperidinojpyrrolidino exchange, isolated as 3· HCI; 4: piperidinoj hexamethylenimino exchange, isolated as 4 · HCl; 5: quaternization of 2b with methyl iodide). The chiral compounds 2a, 2b, 3, 4 and 5 were prepared as racemates. In functional pharmacological studies, 3-5 behaved as simple competitive antagonists at musearlnie Ml receptors in rabbit vas deferens, M2 receptors in guinea-pig atria, and M3 receptors in guinea-pig ileal smooth rnuscle. The pyrrolidino (3) and hexamethylenimino (4) analogues of the parent drug p-F-HHSiD (2b) displayed the highest affinity for Ml and M3 receptors (pA\(_2\) values: 7.0-7.4) but exhibited lower affinity for cardiac M2 receptors (pA\(_2\) : 5.9 and 6.0). Their affinity profile (Ml- M3 > M2) is different from that of p-F-HHSiD (2b) (M3 > Ml > M2), but qualitatively very similar tothat of p-F-HHD (2a). The methiodide 5 exhibited the highest affinity for Ml receptors (pA\(_2\) : 8.5) but lower affinity for M2 and M3 receptors by factors of 5.6 and 3.6, respectively.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{HaasBrehmKreftetal.1991,
  author    = {Haas, Albert and Brehm, Klaus and Kreft, J{\"u}rgen and Goebel, Werner},
  title     = {Cloning, characterization, and expression in Escherichia coli of a gene encoding Listeria seeligeri catalase, a bacterial enzyme highly homologous to mammalian catalases},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60536},
  year      = {1991},
  abstract  = {A gene coding for catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; EC 1.11.1.6) of the grain-positive bacterium Listeria seeligeri was cloned from a plasmid library of EcoRI-digested chromosomal DNA, with Escherichia coli DHSa as a host. The recombinant catalase was expressed in E. coli to an enzymatic activity approximately SO times that of the combined E. coli catalases. The nucleutide sequence was determined, and the deduced amino acid sequence revealed 43.2\% amino acid sequence identity between bovine liver catalase and L. seeligeri catalase. Most of the amino acid residues which are involved in catalytic activity, the formation of the active center accession channel, and heme binding in bovine liver catalase were also present in L. seeligeri catalase at the corresponding positions. The recombinant protein contained 488 amino acid residues and had a calculated molecular weight of 55,869. The predicted isoelectric point was 5.0. Enzymatic and genetic analyses showed that there is most probably a single catalase of this type in L. seeligeri. A perfect 21-bp inverted repeat, which was highly homologous to previously reported binding sequences of the Fur (ferric uptake regulon) protein of E. coli, was detected next to the putative promoter region of tbe L. seeligeri catalase gene.},
  subject      = {Biologie},
  language  = {en}
}
@article{ReuchleinKraftChristletal.1991,
  author    = {Reuchlein, H. and Kraft, A. and Christl, Manfred and Peters, K. and Peters, E.-M. and Schnering, H. G. von},
  title     = {Reaktionen von Bicyclo[2.1.1]hexenen mit 1,3,4-Oxadiazin-6-onen und dynamische Effekte einem in neungliedrigen, {\"u}berbr{\"u}ckten, α,β-unges{\"a}ttigten Enollacton},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58577},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Organische Chemie},
  language  = {de}
}
@article{MarkgrafCortDavisetal.1991,
  author    = {Markgraf, J. H. and Cort, J. R. and Davis, H. A. and Lindeman, N. I. and Myers, C. R. and Kraft, A. and Christl, Manfred},
  title     = {Strained Heterocyclic Systems. 20. Basicities of Bicyclic Quinoxalines},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58584},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{SchueleinKreftGonskietal.1991,
  author    = {Sch{\"u}lein, Ralf and Kreft, J{\"u}rgen and Gonski, Sigrid and Goebel, Werner},
  title     = {Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60577},
  year      = {1991},
  abstract  = {During an investigation into the substrate specificity and processing of subtilisin Carlsberg from Bacillus licheniformis, two major independent findings were made: (i) as has been shown previously, a stretch of five amino acids (residues 97-101 of the mature enzyme) that loops out into the binding cleft is involved in substrate binding by subtilisin Carlsberg. In order to see whether this loop element also determines substrate specificity, the coding region for these five amino acids was deleted from the cloned gene for subtilisin Carlsberg by site-directed mutagenesis. Unexpectedly the resulting mutant preproenzyme (P42c, M<sub>r</sub>=42 kDa) was not processed to the mature form (M<sub>r</sub> = 30 kDa) and was not released into the medium by a proteasedeficient B. subtilis host strain; rather, it accumulated in the cell membrane. This result demonstrates that the integrity of this loop element, which is very distant from the processing cleavage sites in the preproenzyme, is required for secretion of subtilisin Carlsberg. (ii) In culture supernatants from B. subtilis harbouring the cloned wild-type subtilisin Carlsberg gene the transient appearance (at 0-3 h after onset of stationary phase) of a processing intermediate (P38c, M<sub>r</sub> = 38 kDa) oftbis protease could be demonstrated. P38c very probably represents a genuine proform of subtilisin Carlsberg.},
  subject      = {Biologie},
  language  = {en}
}
@article{BentleyChristlNorman1991,
  author    = {Bentley, T. W. and Christl, Manfred and Norman, S. J.},
  title     = {Methanesulfonate/p-Nitrobenzoate and p-Toluenesulfonate/p-Nitrobenzoate Rate Ratios. Solvolyses of 1-Adamantyl and Benzhydryl Substrates},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58599},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{JelinekFinkChristlPetersetal.1991,
  author    = {Jelinek-Fink, H. and Christl, Manfred and Peters, K. and Peters, E.-M. and Schnering, H. G. von},
  title     = {Cycloallene, 7 : Ein Siebenringallen-Dimer: Darstellung aus einem 7,7-Dibromnorcaran-Derivat und Thermolyse},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58603},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Organische Chemie},
  language  = {de}
}
@article{VortkampThiasGessleretal.1991,
  author    = {Vortkamp, A. and Thias, U. and Gessler, Manfred and Rosenkranz, W. and Kroisel, P. M. and Tommerup, N. and Kruger, G. and Gotz, J. and Pelz, L. and Grzeschik, Karl-Heinz},
  title     = {A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59217},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Biochemie},
  language  = {en}
}
@incollection{SchulzeKuenzler1991,
  author    = {Schulze, Hans-Joachim and K{\"u}nzler, Jan},
  title     = {Funktionalistische und systemtheoretische Ans{\"a}tze in der Sozialisationsforschung},
  series = {Neues Handbuch der Sozialisationsforschung},
  booktitle = {Neues Handbuch der Sozialisationsforschung},
  publisher = {Beltz},
  address   = {Weinheim},
  isbn      = {3-407-83113-7},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-128168},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  pages     = {121-136},
  year      = {1991},
  abstract  = {No abstract available.},
  language  = {de}
}
@article{JaiswalLambrechtMutschleretal.1991,
  author    = {Jaiswal, Neelam and Lambrecht, G{\"u}nter and Mutschler, Ernst and Tacke, Reinhold and Malik, Kafait U.},
  title     = {Pharmacological characterization of the vascular muscarinic receptors mediating relaxation and contraction in rabbit aorta},
  series = {Journal of Pharmacology and Experimental Therapeutics},
  volume    = {258},
  journal   = {Journal of Pharmacology and Experimental Therapeutics},
  number    = {3},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-128358},
  pages     = {842-850},
  year      = {1991},
  abstract  = {Studies were performed in the rabbit aortic rings, precontracted with norepinephrine, to determine the subtype(s) of muscarinic receptors involved in endothelium-dependent relaxation and contraction in the absence of endothelium elicited by cholinergic stimuli. Acetylcholine (ACh) and arecaidine propargyl ester (APE), a M2 and M3 agonist, produced a dose-dependent relaxation and contraction in endothelium-intact and endothelium-denuded rabbit aortic rings, respectively. Both of these responses were blocked by the muscarinic receptor antagonist atropine. M1 selective agonist McN-A-343 [4-[N-(3-chlorophenyl)carbamoyloxy]-2-butinyltrimethylammonium+ ++ chloride] did not produce any effect on the tone of precontracted aortic rings. ACh- and APE-induced relaxation in aortic rings with intact endothelium was selectively blocked by M3 receptor antagonists hexahydrosila-difenidol and p-fluoro-hexahydro-sila-difenidol (pA2 of 7.84 and 7.18) but not by M1 antagonist pirenzepine or M2 receptor antagonists AF-DX 116 [11-(2-[(diethylamino)methyl]- 1-piperidinyl]acetyl)-5, 11-dihydro-6H-pyrido-[2,3-b][1,4]-benzo-diazepin-6-one] and methoctramine. ACh- and APE-induced contraction was inhibited by M2 receptor antagonists AF-DX 116 and methoctramine (pA2 of 7.11 and 6.71) but not by pirenzepine, hexahydro-sila-difenidol or p-fluoro-hexahydro-sila-difenidol. ACh- and APE-induced relaxation or contraction were not altered by nicotinic receptor antagonist hexamethonium or cyclooxygenase inhibitor indomethacin. These data suggest that relaxation elicited by cholinergic stimulin in endothelium-intact aortic rings is mediated via release of endothelium-derived relaxing factor consequent to activation of M3 receptors located on endothelial cells, whereas the contraction in aortic rings denuded of their endothelium is mediated via stimulation of M2 receptors located on smooth muscle cells.},
  language  = {en}
}
@misc{OPUS4-3488,
  title     = {Jahresbericht 1990},
  organization    = {Universit{\"a}tsbibliothek W{\"u}rzburg},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42346},
  year      = {1991},
  abstract  = {Die Entwicklung der Universit{\"a}tsbibliothek W{\"u}rzburg im Jahr 1990.},
  subject      = {W{\"u}rzburg},
  language  = {de}
}
@article{KleberSteinAmannetal.1991,
  author    = {Kleber, Eduard W. and Stein, Roland and Amann, Fritz and Brenner, G{\"u}nther},
  title     = {Neue Wege f{\"u}r die Integration Lernbehinderter in die Berufswelt? : Oder neue Wege, um niedrigere Lohngruppen zu schaffen ... ?(Bericht {\"u}ber einen 1989 angelaufenen Modellversuch)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40163},
  year      = {1991},
  abstract  = {In Hessen wurde 1989 ein Modellversuch gestartet. der die Chancen Lernbehinderter f{\"u}r qualifizierte Arbeitspl{\"a}tze im Bereich Metall verbessern soll. Ziel ist die Erarbeitung einer differenzierten Ausbildung im Berufsbild ,Werkzeugmaschinenspaner', einer reduzierten Variante des aktuellen Metallberufes ,Zerspanungsmechaniker·. Dabei geht es auch um die Integration von Arbeiten an computergesteuerten Dreh- und Fr{\"a}smaschinen. Berufsbildungswerke f{\"u}r die Ausbildung Lernund K{\"o}rperbehinderter sowie Betriebe der freien Wirtschaft bilden im Rahmen des Versuchs modellhaft Gruppen von Werkzeugmaschinenspanern aus. Ziele sind Fachkompetenz, Selbst{\"a}ndigkeit, Kooperations- und Kommunikationsf{\"a}higkeit.},
  subject      = {Sonderp{\"a}dagogik / Zeitschrift},
  language  = {de}
}
@article{KleberStein1991,
  author    = {Kleber, Eduard W. and Stein, Roland},
  title     = {Lernbehinderte und CNC-Technologie : Bericht {\"u}ber einen in Deutschland laufenden Modellversuch},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40172},
  year      = {1991},
  abstract  = {Im Rahmen eines Modellversuches sollen in Hessen neue Ausbildungsg{\"a}nge im Metallbereich f{\"u}r Lernbehinderte umgesetzt und evaluiert werden. Dabei geht es insbesondere um eine Pr{\"u}fung der M{\"o}glichkeiten und Grenzen einer Ausbildung an computergesteuerten Dreh- und Fr{\"a}smaschinen. Berufsbildungswerke f{\"u}r die Ausbildung Lern- und K{\"o}rperbehinderter sowie Betriebe der freien Wirtschaft bilden im Rahmen des Versuches modellhaft Gruppen von Werkzeugmaschinenspanern aus. Ziele des p{\"a}dagogischen Rahmenprogrammes sind gr{\"o}sstm{\"o}gliche Fachkompetenz, Selbst{\"a}ndigkeit, Kooperations- und Kommunikationsf{\"a}higkeit. Von dem Projekt sind Erkenntnisse hinsichtlich der M{\"o}glichkeiten des qualifizierten Einsatzes Lernbeeintr{\"a}chtigter in Bereichen moderner industrieller Fertigung zu erwarten.},
  subject      = {Sonderp{\"a}dagogik / Zeitschrift},
  language  = {de}
}
@incollection{Ellgring1991,
  author    = {Ellgring, Johann Heinrich},
  title     = {Das Muskelspiel der Gef{\"u}hle - Zur Verkn{\"u}pfung von Mimik und R{\"u}ckenmuskulatur mit dem subjektiven Erleben},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42984},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  year      = {1991},
  abstract  = {Kaum ein anderer Teil des menschlichen K{\"o}rpers ist in der Lage, gef{\"u}hlsm{\"a}ßige Regungen in derart differenzierter Form auszudr{\"u}cken wie die Mimik. {\"U}ber den Zusammenhang zwischen muskul{\"a}rer Aktivit{\"a}t und Emotionen wird versucht, in einigen Aspekten auch die Beziehung von Stimmungen zur R{\"u}ckenmuskulatur herzustellen. Vor allem wird jedoch auf die Mimik als Verst{\"a}ndigungsmittel eingegangen. Die neuroanatomische und neurophysiologische Basis der Mimik wird dargestellt sowie der Zusammenhang zwischen Stimulus-Situation und Emotionen einerseits und Emotionen und Ausdruck andererseits. St{\"o}rungen dieser Beziehungen werden anhand von psychiatrischen und neurologischen Erkrankungen erl{\"a}utert. Aus dem Ausdruck von Konflikten in der Mimik werden einige Bedingungen f{\"u}r psychosomatische St{\"o}rungen nicht nur im R{\"u}ckenbereich, sondern auch bei Kopfschmerzzust{\"a}nden und Streßverhalten abgeleitet.},
  language  = {de}
}
@article{GigstadRicciJRMarkgrafetal.1991,
  author    = {Gigstad, Kenneth M. and Ricci JR., John S. and Markgraf, J. Hodge and Christl, Manfred and Kraft, Arno},
  title     = {Strained Heterocyclic Systems : 18. Structure of 1,2,3-Methylidyne-2,3-dihydro-1H-cyclopenta[b]quinoxaline},
  isbn      = {0108-2701},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41645},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{Marohn1991,
  author    = {Marohn, Frank},
  title     = {Global sufficiency of extreme order statistics in location models of Weibull type},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47874},
  year      = {1991},
  abstract  = {In Janssen and Reiss (1988) it was shown that in a location model of a Weibull type sample with shape parameter -1 < a < 1 the k(n) lower extremes are asymptotically local sufficient. In the present paper we show that even global sufficiency holds. Moreover, it turns out that convergence of the given statistical experiments in the deficiency metric does not only hold for compact parameter sets but for the whole real line.},
  subject      = {Extremwertstatistik},
  language  = {de}
}
@incollection{HommersAnderson1991,
  author    = {Hommers, Wilfried and Anderson, Norman H.},
  title     = {Moral algebra of harm and recompense},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44046},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Informationsverarbeitung / Kognition},
  language  = {en}
}
@article{Hommers1991,
  author    = {Hommers, Wilfried},
  title     = {Ist das Konzept des Anti-Typus entbehrlich? Eine Antwort des Mischverteilungsansatzes zu einem IPC-Anti-Typ : (Prof. Dr. Dr. G.A. Lienert zum 70. Geburtstag)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44109},
  year      = {1991},
  abstract  = {Durch multidimensional skalierte Strukturen von Pers{\"o}nlichkeitsprofilen wurde eine AlternativHypothese f{\"u}r konfigurationsfrequenzanalytisch erlangte Konfigurationen begr{\"u}ndet. Diese Alternativ- Hypothese besagte, daß die Anti-Typus-Konfigurationen eines dreivariaten 2x2x2- Datenvektors, aber auch die Neutral-Konfigurationen lediglich Folgen der Mischverteilung der Antworten zweier latenter, den Typen-Konfigurationen zuzuordnenden Klassen darstellen. Diese Hypothese wird an einem Datensatz zur Verteilung dichotomisierter Kontroll{\"u}berzeugungen gepr{\"u}ft und best{\"a}tigt. Das dabei konzipierte Konfigurale Fehlermodell (KFM) wird generell zur Erg{\"a}nzung von Konfigurationsfrequenzanalysen (KFA) vorgeschlagen.},
  subject      = {Klinische Psychologie / Zeitschrift /Psychiatrie / Zeitschrift / Klinische Psychiatrie / Psychotherapie},
  language  = {de}
}
@article{Wilhelm1991,
  author    = {Wilhelm, Gernot},
  title     = {Probleme der hethitischen Chronologie},
  series = {Orientalische Literaturzeitung},
  volume    = {86},
  journal   = {Orientalische Literaturzeitung},
  number    = {5/6},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-128499},
  pages     = {469-476},
  year      = {1991},
  abstract  = {No abstract available.},
  subject      = {Hethiter},
  language  = {de}
}
@incollection{Wehgartner1991,
  author    = {Wehgartner, Irma},
  title     = {56. Schale (Typus B)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-129371},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  year      = {1991},
  abstract  = {Katalogbeitrag},
  subject      = {Staatliche Museen zu Berlin. Antikensammlung},
  language  = {de}
}
@incollection{Wehgartner1991,
  author    = {Wehgartner, Irma},
  title     = {59. Schale (Typus B)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-129382},
  publisher      = {Universit{\"a}t W{\"u}rzburg},
  year      = {1991},
  abstract  = {Katalogbeitrag},
  subject      = {Staatliche Museen zu Berlin. Antikensammlung},
  language  = {de}
}
@article{DabauvalleLoosMerkertetal.1991,
  author    = {Dabauvalle, Marie-Christine and Loos, Karin and Merkert, Hilde and Scheer, Ulrich},
  title     = {Spontaneous assembly of pore complex-containing membranes ("Annulate lamellae") in Xenopus egg extract in the absence of chromatin},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32797},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{BeckerWuWaagetal.1991,
  author    = {Becker, Charles R. and Wu, Y. S. and Waag, A. and Kraus, M. M. and Landwehr, G.},
  title     = {The orientation independence of the CdTe-HgTe valence band offset as determined by x-ray photoelectron spectroscopy},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30784},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{MollRoellinghoff1991,
  author    = {Moll, Heidrun and R{\"o}llinghoff, Martin},
  title     = {T-cell reactivity to purified lipophosphoglycan from Leishmania major: A model for analysis of the cellular immune response to microbial carbohydrates.},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33022},
  year      = {1991},
  abstract  = {The major macromolecule on the surface o/Leishmania majorpromastigotes is a lipophosphoglycan (LPG). This glycoconjugate plays a key role in determining infectivity and survival of para-sites in the mammalian host cell. In addition, L. major LPG is able to induce a host-protective immune response. In this article, we summarise the evidence for recognition of highly purified LPG by T cells and we discuss the potential mechanisms of T-cell Stimulation by this non-protein antigen.},
  language  = {en}
}
@techreport{SchuesslervonGehlenMatthesetal.1991,
  author    = {Sch{\"u}ssler, Ulrich and von Gehlen, K. and Matthes, S. and Okrusch, Martin and Richter, P. and R{\"o}hr, C.},
  title     = {Ultramafische Einschaltungen in Metabasiten der KTB-Vorbohrung},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39206},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Kontinentales Tiefbohrprogramm},
  language  = {de}
}
@article{VanDieKramerHackeretal.1991,
  author    = {Van Die, I. and Kramer, C. and Hacker, J{\"o}rg and Bergmans, H. and Jongen, W. and Hoekstra, W.},
  title     = {Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40353},
  year      = {1991},
  abstract  = {F 1 C fimbriae allow uropathogenic Escherichia coli to adhere to specific epithelial surfaces. This adhesive property is probably due to the presence of minor fimbrial components in F1C fimbriae. The foe gene cluster encoding F1C fimbriae has been cloned, as described previously. Here we present the nucleotide sequence (2081 bp) coding for the F 1 C minor fimbria I subunits. The structural genes code for polypeptides of 175 (FocF), 166 (FocG), and 300 (FocH) amino acids. The deduced amino acids of the F 1 C minor subunits were compared with the reported sequences of the minor subunits of other types of fimbriae. The data show that the Foc minor subunits are highly homologous to the corresponding Sfa proteins, whereas homology to the minor subunits of type 1 and P fimbriae is much lower.},
  language  = {en}
}
@article{LueckBenderOttetal.1991,
  author    = {L{\"u}ck, P. Christian and Bender, Larisa and Ott, Manfred and Helbig, J{\"u}rgen H. and Hacker, J{\"o}rg},
  title     = {Analysis of Legionella pneumophila serogroup 6 strains isolated from a hospital warm water supply over a three-year period by using genomic long-range mapping techniques and monoclonal antibodies},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40392},
  year      = {1991},
  abstract  = {Over a period of 3 years, Legionella pneumophila serogroup 6 strains were isolated from warm water outlets and dental units in the Dental Faculty and from the Surgery and Internal Medicine Clinics at the University of Dresden, Dresden, Germany. In the bacteriological unit of the above-mentioned facility, L. pneumophila serogroups 3 and 12 were grown frl,)m warm water specimens. The medical facilities are located in separate buildings connected with a ring pipe warm water system. All L. pneumophila serogroup 6 strains isolated from the warm water supply reacted with a serogroup-specific monoclonal antibody, but not with two other monoclonal antibodies which are subgroup specific, reacting with other serogroup 6 strains. The NolI genomic profiles obtained by pulsed-field gel electrophoresis of 25 serogroup 6 strains isolated from the Dental Faculty over a 3-year period, 1 isolate from the Internal Medicine Clinic, and 4 strains from the Surgery Clinic were identical. Furthermore, all these strains hybridized with a 3OO-kb NolI fragment when a legiolysin (lIy)-specific DNA probe was used. The NolI pattern, however, differed from those of six serogroup 6 strains of other origins, one serogroup 12 strain from the bacteriological unit, and another six unrelated strains of serogroups other than serogroup 6. L. pneumophila serogroup 6 strains which can be divided into only two subgroups by the use of monoclonal antibodies are differentiated in at least six Noli cleavage types obtained by pulsed-field electrophoresis.},
  language  = {en}
}
@misc{FischerWeissenbergerScheer1991,
  author    = {Fischer, Dagmar and Weißenberger, Dieter and Scheer, Ulrich},
  title     = {Assigning functions to nucleolar structures},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34258},
  year      = {1991},
  abstract  = {Nucleoli provide the fascinating possibility of linking morphologically distinct structures such as those seen in the electron microscope with biochemical f eatures of the formation and step wise maturation of ribosomes. Localization of proteins by immunocytochemistry and of rRNA genes and their transcripts by in situ hybridization has greatly improved our understanding of the structural-functional relationships of the nucleolus. The present review describes some recent results obtained by electron microscopic in situ hybridization and argues that this approach has the potential to correlate each step of the complex pre-rRNA maturation pathway with nucleolar structures. Evidence is accumulating that the nucleolus-specific U3 snRNPs (small nuclear ribonucleoprotein particles) participate in rRNA processing events, similar to the role played by the nucleoplasmic snRNPs in mRNA maturation. The intranucleolar distribution of U3 snRNA is consistent with the view that it is involved in both early and late stages of pre-rRNA processing.},
  language  = {en}
}
@article{BringmannZagstSchoeneretal.1991,
  author    = {Bringmann, Gerhard and Zagst, Rainer and Sch{\"o}ner, Bernd and Busse, Holger and Hemmerling, Martin and Burschka, Christian},
  title     = {Acetogenic Isoquinoline Alkaloids. XXIII. Structure of the Naphthyl Isoquinoline Alkaloid Dioncophylline A},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31331},
  year      = {1991},
  abstract  = {No abstract available},
  subject      = {Chemie},
  language  = {en}
}
@misc{Dandekar1991,
  author    = {Dandekar, Thomas},
  title     = {Yeast U3 localization and correct sequence (snR17a) and promotor activity (snR17b) identified by homology search},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29781},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{VortkampGesslerGrzeschik1991,
  author    = {Vortkamp, Andrea and Gessler, Manfred and Grzeschik, Karl-Heinz},
  title     = {GLI3 zinc-finger gene interrupted by translocations in Greig syndrome families},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30100},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {de}
}
@article{DandekarTollervey1991,
  author    = {Dandekar, Thomas and Tollervey, D.},
  title     = {Thirty-three nucleotides of 5' flanking sequence including the TATA box are necessary and sufficient for efficient U2 snRNA transcription in Schizosaccharomycespombe},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29959},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@article{DandekarStripeckeGrayetal.1991,
  author    = {Dandekar, Thomas and Stripecke, Renata and Gray, Nicola K. and Goossen, Britta and Constable, Anne and Johansson, Hans E. and Hentze, Matthias W.},
  title     = {Identification of a novel iron-responsive element in murine and human erythroid \(\delta\)-aminolevulinic acid synthase mRNA},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29929},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}
@misc{DandekarArgos1991,
  author    = {Dandekar, Thomas and Argos, Patrick},
  title     = {Chaperonin-mediated protein folding at the surface of groEL through a "molten globule" intermediate},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29939},
  year      = {1991},
  abstract  = {No abstract available},
  language  = {en}
}