TY  - JOUR
A1  - Britz, Sebastian
A1  - Markert, Sebastian Matthias
A1  - Witvliet, Daniel
A1  - Steyer, Anna Maria
A1  - Tröger, Sarah
A1  - Mulcahy, Ben
A1  - Kollmannsberger, Philip
A1  - Schwab, Yannick
A1  - Zhen, Mei
A1  - Stigloher, Christian
T1  - Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
JF  - Frontiers in Neuroanatomy
N2  - At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.
KW  - FIB-SEM
KW  - 3D reconstruction
KW  - neuroanatomy
KW  - IL2 branching
KW  - amphids
KW  - Caenorhabditis elegans (C. elegans)
KW  - dauer
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-249622
SN  - 1662-5129
VL  - 15
ER  - 
TY  - JOUR
A1  - Müller, Dominik
A1  - Graetz, Jonas
A1  - Balles, Andreas
A1  - Stier, Simon
A1  - Hanke, Randolf
A1  - Fella, Christian
T1  - Laboratory-Based Nano-Computed Tomography and Examples of Its Application in the Field of Materials Research
JF  - Crystals
N2  - In a comprehensive study, we demonstrate the performance and typical application scenarios for laboratory-based nano-computed tomography in materials research on various samples. Specifically, we focus on a projection magnification system with a nano focus source. The imaging resolution is quantified with common 2D test structures and validated in 3D applications by means of the Fourier Shell Correlation. As representative application examples from nowadays material research, we show metallization processes in multilayer integrated circuits, aging in lithium battery electrodes, and volumetric of metallic sub-micrometer fillers of composites. Thus, the laboratory system provides the unique possibility to image non-destructively structures in the range of 170–190 nanometers, even for high-density materials.
KW  - nano CT
KW  - laboratory
KW  - X-ray
KW  - 3D reconstruction
KW  - instrumentation
KW  - integrated circuits
KW  - nondestructive testing
KW  - 3D X-ray microscopy
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-241048
SN  - 2073-4352
VL  - 11
IS  - 6
ER  - 
TY  - JOUR
A1  - Habenstein, Jens
A1  - Amini, Emad
A1  - Grübel, Kornelia
A1  - el Jundi, Basil
A1  - Rössler, Wolfgang
T1  - The brain of Cataglyphis ants: Neuronal organization and visual projections
JF  - Journal of Comparative Neurology
N2  - Cataglyphis ants are known for their outstanding navigational abilities. They return to their inconspicuous nest after far‐reaching foraging trips using path integration, and whenever available, learn and memorize visual features of panoramic sceneries. To achieve this, the ants combine directional visual information from celestial cues and panoramic scenes with distance information from an intrinsic odometer. The largely vision‐based navigation in Cataglyphis requires sophisticated neuronal networks to process the broad repertoire of visual stimuli. Although Cataglyphis ants have been subjected to many neuroethological studies, little is known about the general neuronal organization of their central brain and the visual pathways beyond major circuits. Here, we provide a comprehensive, three‐dimensional neuronal map of synapse‐rich neuropils in the brain of Cataglyphis nodus including major connecting fiber systems. In addition, we examined neuronal tracts underlying the processing of visual information in more detail. This study revealed a total of 33 brain neuropils and 30 neuronal fiber tracts including six distinct tracts between the optic lobes and the cerebrum. We also discuss the importance of comparative studies on insect brain architecture for a profound understanding of neuronal networks and their function.
KW  - 3D reconstruction
KW  - ant brain
KW  - antennal lobes
KW  - central complex
KW  - insect
KW  - mushroom bodies
KW  - optical tracts
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-218212
VL  - 528
IS  - 18
SP  - 3479
EP  - 3506
ER  -