TY  - JOUR
A1  - Eiring, Patrick
A1  - McLaughlin, Ryan
A1  - Matikonda, Siddharth S.
A1  - Han, Zhongying
A1  - Grabenhorst, Lennart
A1  - Helmerich, Dominic A.
A1  - Meub, Mara
A1  - Beliu, Gerti
A1  - Luciano, Michael
A1  - Bandi, Venu
A1  - Zijlstra, Niels
A1  - Shi, Zhen-Dan
A1  - Tarasov, Sergey G.
A1  - Swenson, Rolf
A1  - Tinnefeld, Philip
A1  - Glembockyte, Viktorija
A1  - Cordes, Thorben
A1  - Sauer, Markus
A1  - Schnermann, Martin J.
T1  - Targetable conformationally restricted cyanines enable photon-count-limited applications
JF  - Angewandte Chemie Internationale Edition
N2  - Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications, but their photon output can be limited by trans-to-cis photoisomerization. We recently demonstrated that appending a ring system to the pentamethine cyanine ring system improves the quantum yield and extends the fluorescence lifetime. Here, we report an optimized synthesis of persulfonated variants that enable efficient labeling of nucleic acids and proteins. We demonstrate that a bifunctional sulfonated tertiary amide significantly improves the optical properties of the resulting bioconjugates. These new conformationally restricted cyanines are compared to the parent cyanine derivatives in a range of contexts. These include their use in the plasmonic hotspot of a DNA-nanoantenna, in single-molecule Förster-resonance energy transfer (FRET) applications, far-red fluorescence-lifetime imaging microscopy (FLIM), and single-molecule localization microscopy (SMLM). These efforts define contexts in which eliminating cyanine isomerization provides meaningful benefits to imaging performance.
KW  - biology
KW  - super-resolution microscopy
KW  - conformational restriction
KW  - cyanine dyes
KW  - DNA nanotechnology
KW  - fluorescent dyes
KW  - single-molecule fluorescence spectroscopy
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-256559
VL  - 60
IS  - 51
ER  - 
TY  - JOUR
A1  - Dotterweich, Julia
A1  - Tower, Robert J.
A1  - Brandl, Andreas
A1  - Müller, Marc
A1  - Hofbauer, Lorenz C.
A1  - Beilhack, Andreas
A1  - Ebert, Regina
A1  - Glüer, Claus C.
A1  - Tiwari, Sanjay
A1  - Schütze, Norbert
A1  - Jakob, Franz
T1  - The KISS1 Receptor as an In Vivo Microenvironment Imaging Biomarker of Multiple Myeloma Bone Disease
JF  - PLoS One
N2  - Multiple myeloma is one of the most common hematological diseases and is characterized by an aberrant proliferation of plasma cells within the bone marrow. As a result of crosstalk between cancer cells and the bone microenvironment, bone homeostasis is disrupted leading to osteolytic lesions and poor prognosis. Current diagnostic strategies for myeloma typically rely on detection of excess monoclonal immunoglobulins or light chains in the urine or serum. However, these strategies fail to localize the sites of malignancies. In this study we sought to identify novel biomarkers of myeloma bone disease which could target the malignant cells and/or the surrounding cells of the tumor microenvironment. From these studies, the KISS1 receptor (KISS1R), a G-protein-coupled receptor known to play a role in the regulation of endocrine functions, was identified as a target gene that was upregulated on mesenchymal stem cells (MSCs) and osteoprogenitor cells (OPCs) when co-cultured with myeloma cells. To determine the potential of this receptor as a biomarker, in vitro and in vivo studies were performed with the KISS1R ligand, kisspeptin, conjugated with a fluorescent dye. In vitro microscopy showed binding of fluorescently-labeled kisspeptin to both myeloma cells as well as MSCs under direct co-culture conditions. Next, conjugated kisspeptin was injected into immune-competent mice containing myeloma bone lesions. Tumor-burdened limbs showed increased peak fluorescence compared to contralateral controls. These data suggest the utility of the KISS1R as a novel biomarker for multiple myeloma, capable of targeting both tumor cells and host cells of the tumor microenvironment.
KW  - multiple myeloma Lesions
KW  - fluorescence microscopy
KW  - biomarkers Myelomas
KW  - bone imaging
KW  - myeloma cells
KW  - fluorescent dyes
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-146960
VL  - 11
IS  - 5
ER  -