TY  - JOUR
A1  - Villalobos, Alvaro S.
A1  - Wiese, Jutta
A1  - Imhoff, Johannes F.
A1  - Dorador, Cristina
A1  - Keller, Alexander
A1  - Hentschel, Ute
T1  - Systematic affiliation and genome analysis of Subtercola vilae DB165T with particular emphasis on cold adaptation of an isolate from a high-altitude cold volcano lake
JF  - Microorganisms
N2  - Among the Microbacteriaceae the species of Subtercola and Agreia form closely associated clusters. Phylogenetic analysis demonstrated three major phylogenetic branches of these species. One of these branches contains the two psychrophilic species Subtercola frigoramans and Subtercola vilae, together with a larger number of isolates from various cold environments. Genomic evidence supports the separation of Agreia and Subtercola species. In order to gain insight into the ability of S. vilae to adapt to life in this extreme environment, we analyzed the genome with a particular focus on properties related to possible adaptation to a cold environment. General properties of the genome are presented, including carbon and energy metabolism, as well as secondary metabolite production. The repertoire of genes in the genome of S. vilae DB165\(^T\) linked to adaptations to the harsh conditions found in Llullaillaco Volcano Lake includes several mechanisms to transcribe proteins under low temperatures, such as a high number of tRNAs and cold shock proteins. In addition, S. vilae DB165\(^T\) is capable of producing a number of proteins to cope with oxidative stress, which is of particular relevance at low temperature environments, in which reactive oxygen species are more abundant. Most important, it obtains capacities to produce cryo-protectants, and to combat against ice crystal formation, it produces ice-binding proteins. Two new ice-binding proteins were identified which are unique to S. vilae DB165\(^T\). These results indicate that S. vilae has the capacity to employ different mechanisms to live under the extreme and cold conditions prevalent in Llullaillaco Volcano Lake.
KW  - cold adaptation
KW  - Subtercola vilae
KW  - genome analysis
KW  - systematic affiliation
KW  - Llullaillaco Volcano
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-197394
SN  - 2076-2607
VL  - 7
IS  - 4
ER  - 
TY  - JOUR
A1  - Kunz, Anna Lena
A1  - Labes, Antje
A1  - Wiese, Jutta
A1  - Bruhn, Torsten
A1  - Bringmann, Gerhard
A1  - Imhoff, Johannes F.
T1  - Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain
JF  - Marine Drugs
N2  - Eight streptophenazines (A-H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I-K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I-K) also showed moderate activities against PDE 4B.
KW  - marine natural product
KW  - phenazine
KW  - Baltic Sea
KW  - streptomyces
KW  - halichondria panicea
KW  - obstructive pulmonary disease
KW  - phosphodiesterase-4 inhibitor
KW  - absolute configuration
KW  - biosynthesis
KW  - rofumilast
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116816
SN  - 1660-3397
VL  - 12
IS  - 4
ER  -