TY  - JOUR
A1  - Engert, Jonas
A1  - Doll, Julia
A1  - Vona, Barbara
A1  - Ehret Kasemo, Totta
A1  - Spahn, Bjoern
A1  - Hagen, Rudolf
A1  - Rak, Kristen
A1  - Voelker, Johannes
T1  - mRNA abundance of neurogenic factors correlates with hearing capacity in auditory brainstem nuclei of the rat
JF  - Life
N2  - Neural stem cells (NSCs) have previously been described up to the adult stage in the rat cochlear nucleus (CN). A decreasing neurogenic potential was observed with critical changes around hearing onset. A better understanding of molecular factors affecting NSCs and neurogenesis is of interest as they represent potential targets to treat the cause of neurologically based hearing disorders. The role of genes affecting NSC development and neurogenesis in CN over time on hearing capacity has remained unclear. This study investigated the mRNA abundance of genes influencing NSCs and neurogenesis in rats’ CN over time. The CN of rats on postnatal days 6, 12, and 24 were examined. Real-time quantitative polymerase chain reaction arrays were used to compare mRNA levels of 84 genes relevant to NSCs and neurogenesis. Age- and hearing-specific patterns of changes in mRNA abundance of neurogenically relevant genes were detected in the rat CN. Additionally, crucial neurogenic factors with significant and relevant influence on neurogenesis were identified. The results of this work should contribute to a better understanding of the molecular mechanisms underlying the neurogenesis of the auditory pathway.
KW  - real-time PCR
KW  - auditory pathway
KW  - neurogenesis
KW  - cochlear nucleus
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-357392
SN  - 2075-1729
VL  - 13
IS  - 9
ER  - 
TY  - JOUR
A1  - Moratin, Helena
A1  - Thöle, Anna
A1  - Lang, Josephine
A1  - Ehret Kasemo, Totta
A1  - Stöth, Manuel
A1  - Hagen, Rudolf
A1  - Scherzad, Agmal
A1  - Hackenberg, Stephan
T1  - Ag- but not ZnO-nanoparticles disturb the airway epithelial barrier at subtoxic concentrations
JF  - Pharmaceutics
N2  - Inhalation is considered to be the most relevant source of human exposure to nanoparticles (NPs); however, only a few investigations have addressed the influence of exposing the respiratory mucosal barrier to subcytotoxic doses. In the nasal respiratory epithelium, cells of the mucosa represent one of the first contact points of the human organism with airborne NPs. Disruption of the epithelial barrier by harmful materials can lead to inflammation in addition to potential intrinsic toxicity of the particles. The aim of this study was to investigate whether subtoxic concentrations of zinc oxide (ZnO)- and silver (Ag)-NPs have an influence on upper airway barrier integrity. Nasal epithelial cells from 17 donors were cultured at the air–liquid interface and exposed to ZnO- and Ag-NPs. Barrier function, quantified by transepithelial electrical resistance (TEER), decreased after treatment with 10 µg/mL Ag-NPs, but FITC-dextran permeability remained stable and no change in mRNA levels of tight junction proteins and E-cadherin was detected by real-time quantitative PCR (RT-qPCR). The results indicate that subtoxic concentrations of Ag-NPs may already induce damage of the upper airway epithelial barrier in vitro. The lack of similar disruption by ZnO-NPs of similar size suggests a specific effect by Ag-NPs.
KW  - epithelial barrier
KW  - nanoparticles
KW  - tight junctions
KW  - zinc oxide
KW  - silver
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-357403
SN  - 1999-4923
VL  - 15
IS  - 10
ER  -