TY  - JOUR
A1  - Jaschke, Alexander
A1  - Chung, Bomee
A1  - Hesse, Deike
A1  - Kluge, Reinhart
A1  - Zahn, Claudia
A1  - Moser, Markus
A1  - Petzke, Klaus-Jürgen
A1  - Brigelius-Flohé, Regina
A1  - Puchkov, Dmytro
A1  - Koepsell, Hermann
A1  - Heeren, Joerg
A1  - Joost, Hans-Georg
A1  - Schürmann, Annette
T1  - The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium
JF  - Human Molecular Genetics
N2  - The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine \((Arfrp1^{vil−/−})\) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. \(Arfrp1^{vil−/−}\) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted chylomicrons with a markedly reduced triacylglycerol content. In addition, the release of apolipoprotein A-I (ApoA-I) was dramatically decreased, and ApoA-I accumulated in the \(Arfrp1^{vil−/−}\) epithelium, where it predominantly co-localized with Rab2. The release of chylomicrons from Caco-2 was markedly reduced after the suppression of Rab2, ARL1 and Golgin-245. Thus, the GTPase ARFRP1 and its downstream proteins are required for the lipidation of chylo­microns and the assembly of ApoA-I to these particles in the Golgi of intestinal epithelial cells.
KW  - ARF
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-125658
VL  - 21
IS  - 14
ER  - 
TY  - JOUR
A1  - Kessler, Michael
A1  - Hertel, Dietrich
A1  - Jungkunst, Hermann F.
A1  - Kluge, Jürgen
A1  - Abrahamczyk, Stefan
A1  - Bos, Merijn
A1  - Buchori, Damayanti
A1  - Gerold, Gerhard
A1  - Gradstein, S. Robbert
A1  - Köhler, Stefan
A1  - Leuschner, Christoph
A1  - Moser, Gerald
A1  - Pitopang, Ramadhanil
A1  - Saleh, Shahabuddin
A1  - Schulze, Christian H.
A1  - Sporn, Simone G.
A1  - Steffan-Dewenter, Ingolf
A1  - Tjitrosoedirdjo, Sri S.
A1  - Tscharntke, Teja
T1  - Can Joint Carbon and Biodiversity Management in Tropical Agroforestry Landscapes Be Optimized?
JF  - PLoS One
N2  - Managing ecosystems for carbon storage may also benefit biodiversity conservation, but such a potential 'win-win' scenario has not yet been assessed for tropical agroforestry landscapes. We measured above-and below-ground carbon stocks as well as the species richness of four groups of plants and eight of animals on 14 representative plots in Sulawesi, Indonesia, ranging from natural rainforest to cacao agroforests that have replaced former natural forest. The conversion of natural forests with carbon stocks of 227-362 Mg C ha\(^{-1}\) to agroforests with 82-211 Mg C ha\(^{-1}\) showed no relationships to overall biodiversity but led to a significant loss of forest-related species richness. We conclude that the conservation of the forest-related biodiversity, and to a lesser degree of carbon stocks, mainly depends on the preservation of natural forest habitats. In the three most carbon-rich agroforestry systems, carbon stocks were about 60% of those of natural forest, suggesting that 1.6 ha of optimally managed agroforest can contribute to the conservation of carbon stocks as much as 1 ha of natural forest. However, agroforestry systems had comparatively low biodiversity, and we found no evidence for a tight link between carbon storage and biodiversity. Yet, potential win-win agroforestry management solutions include combining high shade-tree quality which favours biodiversity with cacao-yield adapted shade levels.
KW  - forest soils
KW  - stocks
KW  - diversity
KW  - sequestration
KW  - conversion
KW  - balance
KW  - root
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-132016
VL  - 7
IS  - 10
ER  -