TY  - THES
A1  - Bönhof, Leoni Jacobea
T1  - Herzinfarkt-scheinoperierte Ratten vs. gesunde Ratten: Ein Vergleich in der Cine-Herzmagnetresonanztomografie
T1  - Heart infarct Sham-operated rats vs. healthy rats - A comparison in Cine Magnetic resonance imaging
N2  - In der vorliegenden Arbeit wurde überprüft, ob sich sham-operierte Versuchsratten und gesunde Vergleichsratten in via Cine-Herz-MRT zu erfassenden Parametern signifkant unterscheiden. Hierzu wurden an einem Bruker 7 Tesla-Magnetresonanztomografen drei verschiedene Tiergruppen à 6 Tiere untersucht. Der erste Vergleich fand statt zwischen der gesunden Vergleichsgruppe und einer ähnlich schweren Shamtiergruppe, die sich in der 8. postoperativen Woche befand. Nachdem hier keinerlei signifikante Unterschiede zwischen den beiden Gruppen festzustellen waren, wurde der Vergleich ausgeweitet: Die Shamgruppe wurde zu einem frühen postoperativen Zeitpunkt (7-14 Tage postoperativ) ein zweites Mal mit der gesunden Gruppe verglichen.
N2  - The aim of this study was to test whether heart infarction sham operated rats were disparable from healthy rats by parameters captured by Cine MRI, gold standard of cardiac imaging. Therefore three groups of animals were examined, right and left ventricular myocardial parameters were determined and a comprehensive comparison of cardiac parameters was done. The first comparison was drawn between a healthy animal group and sham operated animals of nearly similar weight and age, 8 weeks after surgical procedure. Furthermore, we compared the same healthy animals’ group with remarkably younger sham animals quite shortly (in the 2nd week) after surgery.
KW  - Herz
KW  - MRT
KW  - Cine
KW  - Ratte
KW  - Sham
KW  - Myokard
KW  - Infarkt
KW  - Cine
KW  - MRI
KW  - rats
KW  - heart
KW  - infarction
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-71520
ER  - 
TY  - JOUR
A1  - Nordbeck, Peter
A1  - Bönhof, Leoni
A1  - Hiller, Karl-Heinz
A1  - Voll, Sabine
A1  - Arias-Loza, Paula
A1  - Seidlmaier, Lea
A1  - Williams, Tatjana
A1  - Ye, Yu-Xiang
A1  - Gensler, Daniel
A1  - Pelzer, Theo
A1  - Ertl, Georg
A1  - Jakob, Peter M.
A1  - Bauer, Wolfgang R.
A1  - Ritter, Oliver
T1  - Impact of Thoracic Surgery on Cardiac Morphology and Function in Small Animal Models of Heart Disease: A Cardiac MRI Study in Rats
JF  - PLoS ONE
N2  - Background
Surgical procedures in small animal models of heart disease might evoke alterations in cardiac morphology and function. The aim of this study was to reveal and quantify such potential artificial early or long term effects in vivo, which might account for a significant bias in basic cardiovascular research, and, therefore, could potentially question the meaning of respective studies.

Methods
Female Wistar rats (n = 6 per group) were matched for weight and assorted for sham left coronary artery ligation or control. Cardiac morphology and function was then investigated in vivo by cine magnetic resonance imaging at 7 Tesla 1 and 8 weeks after the surgical procedure. The time course of metabolic and inflammatory blood parameters was determined in addition.

Results
Compared to healthy controls, rats after sham surgery showed a lower body weight both 1 week (267.5±10.6 vs. 317.0±11.3 g, n<0.05) and 8 weeks (317.0±21.1 vs. 358.7±22.4 g, n<0.05) after the intervention. Left and right ventricular morphology and function were not different in absolute measures in both groups 1 week after surgery. However, there was a confined difference in several cardiac parameters normalized to the body weight (bw), such as myocardial mass (2.19±0.30/0.83±0.13 vs. 1.85±0.22/0.70±0.07 mg left/right per g bw, p<0.05), or enddiastolic ventricular volume (1.31±0.36/1.21±0.31 vs. 1.14±0.20/1.07±0.17 µl left/right per g bw, p<0.05). Vice versa, after 8 weeks, cardiac masses, volumes, and output showed a trend for lower values in sham operated rats compared to controls in absolute measures (782.2±57.2/260.2±33.2 vs. 805.9±84.8/310.4±48.5 mg, p<0.05 for left/right ventricular mass), but not normalized to body weight. Matching these findings, blood testing revealed only minor inflammatory but prolonged metabolic changes after surgery not related to cardiac disease.

Conclusion
Cardio-thoracic surgical procedures in experimental myocardial infarction cause distinct alterations upon the global integrity of the organism, which in the long term also induce circumscribed repercussions on cardiac morphology and function. This impact has to be considered when analyzing data from respective animal studies and transferring these findings to conditions in patients.
KW  - heart rate
KW  - body weight
KW  - surgical and invasive medical procedures
KW  - magnetic resonance imaging
KW  - blood
KW  - vascular surgery
KW  - myocardial infarction
KW  - cardiac ventricles
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-130064
VL  - 8
IS  - 8
ER  -