TY  - JOUR
A1  - Scholz, Nicole
A1  - Gehring, Jennifer
A1  - Guan, Chonglin
A1  - Ljaschenko, Dmitrij
A1  - Fischer, Robin
A1  - Lakshmanan, Vetrivel
A1  - Kittel, Robert J.
A1  - Langenhan, Tobias
T1  - The adhesion GPCR Latrophilin/CIRL shapes mechanosensation
JF  - Cell Reports
N2  - G-protein-coupled receptors (GPCRs) are typically regarded as chemosensors that control cellular states in response to soluble extracellular cues. However, the modality of stimuli recognized through adhesion GPCR (aGPCR), the second largest class of the GPCR superfamily, is unresolved. Our study characterizes the Drosophila aGPCR Latrophilin/dCirl, a prototype member of this enigmatic receptor class. We show that dCirl shapes the perception of tactile, proprioceptive, and auditory stimuli through chordotonal neurons, the principal mechanosensors of Drosophila. dCirl sensitizes these neurons for the detection of mechanical stimulation by amplifying their input-output function. Our results indicate that aGPCR may generally process and modulate the perception of mechanical signals, linking these important stimuli to the sensory canon of the GPCR superfamily.
KW  - \(\alpha\)-latrotoxin
KW  - chordotonal organs
KW  - Johnstons organ
KW  - ligand CD55
KW  - hearing
KW  - binding
KW  - shear stress
KW  - protein-coupled receptors
KW  - drosophila larvae
KW  - domain
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-148626
VL  - 11
ER  - 
TY  - JOUR
A1  - Fux, Robert
A1  - Arndt, Daniela
A1  - Langenmayer, Martin C.
A1  - Schwaiger, Julia
A1  - Ferling, Hermann
A1  - Fischer, Nicole
A1  - Indenbirken, Daniela
A1  - Grundhoff, Adam
A1  - Dölken, Lars
A1  - Adamek, Mikolaj
A1  - Steinhagen, Dieter
A1  - Sutter, Gerd
T1  - Piscine orthoreovirus 3 is not the causative pathogen of proliferative darkening syndrome (PDS) of brown trout (Salmo trutta fario)
JF  - Viruses
N2  - The proliferative darkening syndrome (PDS) is a lethal disease of brown trout (Salmo trutta fario) which occurs in several alpine Bavarian limestone rivers. Because mortality can reach 100%, PDS is a serious threat for affected fish populations. Recently, Kuehn and colleagues reported that a high throughput RNA sequencing approach identified a piscine orthoreovirus (PRV) as a causative agent of PDS. We investigated samples from PDS-affected fish obtained from two exposure experiments performed at the river Iller in 2008 and 2009. Using a RT-qPCR and a well-established next-generation RNA sequencing pipeline for pathogen detection, PRV-specific RNA was not detectable in PDS fish from 2009. In contrast, PRV RNA was readily detectable in several organs from diseased fish in 2008. However, similar virus loads were detectable in the control fish which were not exposed to Iller water and did not show any signs of the disease. Therefore, we conclude that PRV is not the causative agent of PDS of brown trout in the rhithral region of alpine Bavarian limestone rivers. The abovementioned study by Kuehn used only samples from the exposure experiment from 2008 and detected a subclinical PRV bystander infection. Work is ongoing to identify the causative agent of PDS.
KW  - proliferative darkening syndrome
KW  - black trout syndrome
KW  - piscine orthoreovirus
KW  - orthoreovirus
KW  - brown trout
KW  - Salmo trutta fario
KW  - next generation sequencing
KW  - RT-qPCR
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-196991
SN  - 1999-4915
VL  - 11
IS  - 2
ER  -