TY  - THES
A1  - Schlücker, Sebastian
T1  - Lineare und nichtlineare Raman-Spektroskopie an biologisch relevanten Modellystemen
T1  - Linear and nonlinear Raman spectroscopy on biological relevant model systems
N2  - Im Rahmen dieser Dissertation wurden insgesamt drei verschiedene Fragestellungen an biologisch relevanten Modellsystemen mit Hilfe von diversen linearen und nichtlinearen Raman-spektroskopischen Techniken bearbeitet. Neben der Untersuchung von Wasserstoffbrücken-gebundenen Komplexen und ihrer Dynamik auf der fs-Zeitskala (Kapitel 4) bildeten Untersuchungen zur Struktur von Porphyrinen (Kapitel 5) und beta-Carotin (Kapitel 6) als Vertreter wichtiger Klassen von Biomolekülen den Schwerpunkt dieser Arbeit. Die spektroskopischen Ergebnisse wurden durchweg über Strukturen und Schwingungsspektren, welche mit Hilfe der Dichtefunktionaltheorie (DFT) berechnet wurden, unterstützt. Die dritte bearbeitete Thematik zum Nachweis anthropogener und ökologisch relevanter Aerosole war bioanalytisch motiviert und wurde anhand von Pestizid-Modellsubstanzen bearbeitet (Kapitel 7).
N2  - Three different problems on biological relevant model systems were investigated within the framework of this thesis by means of various linear and nonlinear Raman techniques. Besides the studies on hydrogen-bonded complexes and their dynamics on a fs-timescale (chapter 4), structural investigations on porphyrins (chapter 5) and beta-carotene (chapter 6) as representatives of important classes of biomolecules were the central subject of this dissertation. The experimental data were confirmed throughout by structures and vibrational spectra obtained from calculations based on density functional theory (DFT). The last topic, the detection of anthropogenic aerosols with ecological relevance, was motivated from bioanalytical chemistry. The corresponding investigations were carried out on pesticide model compounds (chapter 7).
KW  - Biomolekül
KW  - CARS-Spektroskopie
KW  - Infrarot- und Raman-Spektroskopie
KW  - Resonanz-Raman-Spektroskopie
KW  - kohärente anti-Stokessche Raman-Spektroskopie (CARS)
KW  - Infrared and Raman spectroscopy
KW  - resonance Raman scattering
KW  - coherent anti-Stokes Raman scattering (CARS)
Y1  - 2001
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-1181438
ER  - 
TY  - JOUR
A1  - Stepula, Elzbieta
A1  - König, Matthias
A1  - Wang, Xin‐Ping
A1  - Levermann, Janina
A1  - Schimming, Tobias
A1  - Kasimir‐Bauer, Sabine
A1  - Schilling, Bastian
A1  - Schlücker, Sebastian
T1  - Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles
JF  - Journal of Biophotonics
N2  - Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies.
KW  - gold nanoparticles
KW  - PD‐L1
KW  - Raman
KW  - SERS
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-212655
VL  - 13
IS  - 3
ER  -