TY  - JOUR
A1  - Bandyra, Katarzyna J.
A1  - Said, Nelly
A1  - Pfeiffer, Verena
A1  - Górna, Maria W.
A1  - Vogel, Jörg
A1  - Luisi, Ben F.
T1  - The Seed Region of a Small RNA Drives the Controlled Destruction of the Target mRNA by the Endoribonuclease RNase E
JF  - Molecular Cell
N2  - Numerous small non-coding RNAs (sRNAs) in bacteria modulate rates of translation initiation and degradation of target mRNAs, which they recognize through base-pairing facilitated by the RNA chaperone Hfq. Recent evidence indicates that the ternary complex of Hfq, sRNA and mRNA guides endoribonuclease RNase E to initiate turnover of both the RNAs. We show that a sRNA not only guides RNase E to a defined site in a target RNA, but also allosterically activates the enzyme by presenting a monophosphate group at the 5′-end of the cognate-pairing “seed.” Moreover, in the absence of the target the 5′-monophosphate makes the sRNA seed region vulnerable to an attack by RNase E against which Hfq confers no protection. These results suggest that the chemical signature and pairing status of the sRNA seed region may help to both ‘proofread’ recognition and activate mRNA cleavage, as part of a dynamic process involving cooperation of RNA, Hfq and RNase E.
KW  - medicine
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-126202
VL  - 47
IS  - 6
ER  - 
TY  - JOUR
A1  - Brixner, T.
A1  - Aeschlimann, M.
A1  - Fischer, A.
A1  - Geisler, P.
A1  - Goetz, S.
A1  - Hecht, B.
A1  - Huang, J. S.
A1  - Keitzl, T.
A1  - Kramer, C.
A1  - Melchior, P.
A1  - Pfeiffer, W.
A1  - Razinskas, G.
A1  - Rewitz, C.
A1  - Schneider, C.
A1  - Strüber, C.
A1  - Tuchscherer, P.
A1  - Voronine, D. V.
T1  - Coherent spectroscopies on ultrashort time and length scales
JF  - EPJ Web of Conferences
N2  - Three spectroscopic techniques are presented that provide simultaneous spatial and temporal resolution: modified confocal microscopy with heterodyne detection, space-time-resolved spectroscopy using coherent control concepts, and coherent two-dimensional nano-spectroscopy. Latest experimental results are discussed.
KW  - coherent spectroscopy
KW  - ultrashort time
KW  - length scale
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-129073
VL  - 41
ER  -