TY  - JOUR
A1  - Otto, Wolfgang
A1  - Rubenwolf, Peter C.
A1  - Burger, Maximilian
A1  - Fritsche, Hans-Martin
A1  - Rößler, Wolfgang
A1  - May, Matthias
A1  - Hartmann, Arndt
A1  - Hofstädter, Ferdinand
A1  - Wieland, Wolf F.
A1  - Denzinger, Stefan
T1  - Loss of aquaporin 3 protein expression constitutes an independent prognostic factor for progression-free survival: an immunohistochemical study on stage pT1 urothelial bladder cancer
JF  - BMC Cancer
N2  - Background: Treatment of patients with stage pT1 urothelial bladder cancer (UBC) continues to be a challenge due to its unpredictable clinical course. Reliable molecular markers that help to determine appropriate individual treatment are still lacking. Loss of aquaporin (AQP) 3 protein expression has previously been shown in muscle-invasive UBC. The aim of the present study was to investigate the prognostic value of AQP3 protein expression with regard to the prognosis of stage pT1 UBC. 
Method: AQP 3 protein expression was investigated by immunohistochemistry in specimens of 87 stage T1 UBC patients, who were diagnosed by transurethral resection of the bladder (TURB) and subsequent second resection at a high-volume urological centre between 2002 and 2009. Patients underwent adjuvant instillation therapy with Bacillus Calmette-Guerin (BCG). Loss of AQP3 protein expression was defined as complete absence of the protein within the whole tumour. Expression status was correlated retrospectively with clinicopathological and follow-up data (median: 31 months). Multivariate Cox regression analysis was used to assess the value of AQP3 tumour expression with regard to recurrence-free (RFS), progression-free (PFS) and cancer-specific survival (CSS). RFS, PFS and CSS were calculated by Kaplan-Meier analysis and Log rank test. 
Results: 59% of patients were shown to exhibit AQP3-positive tumours, whereas 41% of tumours did not express the marker. Loss of AQP3 protein expression was associated with a statistically significantly worse PFS (20% vs. 72%, p=0.020). This finding was confirmed by multivariate Cox regression analysis (HR 7.58, CI 1.29 - 44.68; p=0.025). 
Conclusions: Loss of AQP3 protein expression in pT1 UBC appears to play a key role in disease progression and is associated with worse PFS. Considering its potential prognostic value, assessment of AQP3 protein expression could be used to help stratify the behavior of patients with pT1 UBC.
KW  - urothelial bladder carcinoma
KW  - progression
KW  - transitional cell carcinoma
KW  - bacillus calmette guerin
KW  - water channels
KW  - follow up
KW  - in vitro
KW  - recurrence
KW  - growth
KW  - T1
KW  - tumor
KW  - proliferation
KW  - stage pT1
KW  - aquaporin 3 protein
KW  - immunohistochemistry
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-135679
VL  - 12
IS  - 459
ER  - 
TY  - JOUR
A1  - Al-Janabi, Omar
A1  - Taubert, Helge
A1  - Lohse-Fischer, Andrea
A1  - Fröhner, Michael
A1  - Wach, Sven
A1  - Stöhr, Robert
A1  - Keck, Bastian
A1  - Burger, Max
A1  - Wieland, Wolf
A1  - Erdmann, Kati
A1  - Wirth, Manfred P.
A1  - Wullich, Bernd
A1  - Baretton, Gustavo
A1  - Magdolen, Viktor
A1  - Kotzsch, Mathias
A1  - Füssel, Susanne
T1  - Association of Tissue mRNA and Serum Antigen Levels of Members of the Urokinase-Type Plasminogen Activator System with Clinical and Prognostic Parameters in Prostate Cancer
JF  - Biomed Research International
N2  - The objective was to determine the mRNA expression and protein levels of uPA system components in tissue specimens and serum samples, respectively, from prostate cancer (PCa) patients and to assess their association with clinicopathological parameters and overall survival (OS). The mRNA expression levels of uPA, its receptor (uPAR), and its inhibitor type 1 (PAI-1) were analyzed in corresponding malignant and adjacent nonmalignant tissue specimens from 132 PCa patients by quantitative PCR. Preoperative serum samples from 81 PCa patients were analyzed for antigen levels of uPA system members by ELISA. RNA levels of uPA system components displayed significant correlations with each other in the tumor tissues. A significantly decreased uP AmRNA expression in PCa compared to the corresponding nonmalignant tissue was detected. High uPA mRNA level was significantly associated with a high Gleason score. Elevated concentration of soluble uPAR (suPAR) in serum was significantly associated with a poor OS of PCa patients (P = 0.022). PCa patients with high suPAR levels have a significantly higher risk of death (multivariate Cox's regression analysis; IIR - 7.12, P - 0.027). The association of high suPAR levels with poor survival of PCa patients suggests a prognostic impact of suPAR levels in serum of cancer patients.
KW  - receptor splice variant
KW  - primary breast cancer
KW  - radical prostatectomy
KW  - tumor tissue
KW  - progression
KW  - potential marker
KW  - inhibitor PAI-1
KW  - gastric cancer
KW  - biomarkers UPA
KW  - expression
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-117967
SN  - 2314-6141
IS  - 972587
ER  -