TY - JOUR A1 - Kekow, J. A1 - Ulrichs, Karin T1 - Determination of total immunoreactive rat insulin (IRI) in culture supernatants of rat islets by an enzyme linked immunosorbent assay (ELISA) as a routine method to assess the viability of rat islets prior to their use in islet transplantation N2 - No abstract available KW - Immunobiologie Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72989 ER - TY - JOUR A1 - Engemann, R. A1 - Gassel, HJ A1 - Ulrichs, Karin A1 - Thiede, A. A1 - Hamelmann, H. T1 - Suppressorzellmechanismen nach Cyclosporin A (CsA) induzierter Toleranz allogener Rattenlebertransplantate T1 - Suppressor cell mechanisms after cyclosporin A (CsA) induced tolerance of allogeneic rat liver transplants N2 - No abstract available Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45503 ER - TY - JOUR A1 - Deltz, Eberhard A1 - Ulrichs, Karin A1 - Schack, Thorsten A1 - Friedrichs, Birgit A1 - Müller-Ruchholtz, Wolfgang A1 - Müller-Hermelink, Hans K. A1 - Thiede, Arnulf T1 - Graft-versus-host reaction in small bowel transplantation and possibilities for its circumvention N2 - No abstract available KW - Dünndarm KW - Transplantation KW - transplantation KW - small bowel Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64425 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Keller, R. A1 - Müller-Ruchholtz, W. T1 - Vorkommen und Manipulation von MHC Klasse II Antigenen auf Zellen isolierter Langerhans-Inseln T1 - Presence and manipulation of MHC class II antigens on isolated island of Langerhans cells N2 - No abstract available KW - Langerhans-Inseln Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45499 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Keller, R. A1 - Müller-Ruchholtz, W. T1 - Serological demonstration and manipulation of passenger cells (PC) in pancreas islet grafts N2 - No abstract available KW - Immunobiologie Y1 - 1985 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72944 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Further Analyses of Pancreas Islet Immunogenicity, a Major Barrier to Successful Islet Transplantation N2 - No abstract available KW - Chirurgie Y1 - 1985 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45563 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Müller-Buchholtz, W. T1 - MHC class II antigen expression on the various cells of normal and activated isolated pancreatic islets N2 - It is the aim of this study to characterize and quantify the cells within isolated rat islets that express MHC class 11 antigens. A set of five monoelonal antibodies and two polyclonal antisera of defined specificlty were used in combination with a newly devised procedure for three·dimensional immunofluorescence evaluation of intact islets. It is shown that in addition to passen· ger cells, such as Iymphocytes, macro· phages, and dendrlticlike cells, vascular endothelial and endocrine cells are also capable of expressing class 11 antigens. This expression Is strongly influenced by in vitro culture. pregnancy, streptozotocin- induced diabetes, transplantation trauma, and alloantigenic stimuli. The pos· sible role of the above cells in antigen presentation related to islet transplantation is discussed. KW - pancreas Islets KW - beta cells KW - islet transplantation KW - la antigens Y1 - 1985 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44722 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Schang, T. A1 - Keller, R. A1 - Müller-Ruchholtz, W. T1 - Reactivity of pancreas islet cells with antisera of known specificity N2 - No abstract available Y1 - 1984 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45466 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Yu, MY A1 - Duncker, D. A1 - Müller-Ruchholtz, W. T1 - Immunosuppression by cytostatic drugs? N2 - In the present study, an attempt was made to characterize the immunomodulating abilities of the cytostatic drugs cydophosphamide, ifosfamide, vinblastine, vincristine, procarbazine, dacarbazine, 6-mercaptopurine, methotrexate, 5-f/uor-uracil and adriamycine in a defined experimental model. Varying combinations of drug plus transplantation alloantigen, (C3H-lymphocytes) were injected into Balb/c mice at different time intervals in vivo. The resulting T-effector cell reactivity was determined in vitro with the microcytotoxicity assay on day + 5 for primary (r) and day + 7 for secondary (2°) sensitized mice. According to the type of drug (alkylating agent vs. vinca alkaloid vs. antimetabolite vs. cytostatic antibiotic), the dosage (20% LD50 vs. 60% LD50), the state of sensitization (r vs. 2° sensitized recipients), and the time of drug application in relation to the antigen treatment on day 0 (in varying steps from day -6 to day +4), so-called "pharmaconantigen- variation-effects" (PA VE) were established for each of the investigated drugs in form of reaction profiles. The results were as folIows: (1) For almost alt substances, characteristic reaction profiles involving immunostimulation and/or immunosuppression could be established. Similarities in the profiles of different substances made it possible to classify the drugs according to different reaction types. The reaction type however is not definitely correlated to the biochemical mechanism of drug action. (2) The PA VE are decisively inf/uenced by so me of the biological parameters, such as the time of drug application in relation to the antigen treatment and the state of sensitization but relatively !ittle by the dosage of the drug. (3) Considering the different processes occurring du ring primary and secondary immune responses, the PAVE may give hints for a distinct manipulation of the immunoregulation and thus information on the immunobiological mechanism of drug action. KW - Immunologie Y1 - 1984 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45574 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Deltz, E. A1 - Thiede, A. A1 - Müller-Ruchholtz, W. T1 - Lymphoid tissue transplantation in rats leads to a GVHR, inducing a specific T-cell mediated autoreactivity against MHC-antigens N2 - No abstract available Y1 - 1982 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45420 ER - TY - JOUR A1 - Deltz, E. A1 - Müller-Hermelink, HK A1 - Ulrichs, Karin A1 - Thiede, A. A1 - Müller-Ruchholtz, W. T1 - Development of graft-versus-host reaction in various target organs after small intestine transplantation N2 - The GVHRIL foHowing transplantation of small intestine are different from those found after bone marrow transplantation or spleen cell injections in that they show a remarka ble, significant prevalence of lesions within the intestinal mucosa. These findings are consistent with the observation that jntestinal lymphocytes newly formed in mesenteric lymph nodes predominantly home in on the intestine again.& The degree of histologic alteration within different tissues indicates that the graft and the host may survive the lesions of the lymphatic tissues, whereas the severe intestinal lesions following GVHR may easily cause death of the recipient. With regard to clinical sman bowel transplantation two statements can be made: (l) GVHRIL play a significant role in small bowel trans~ plantation. (2) To minimize their biologic importance, a selective elimination of the graft's Jymph nodes by irradiation or surgical resection should be considered in view of the remarkable difference between GVHRIL in lymph nodes and in the graft's intestinal wall itself. KW - Chirurgie Y1 - 1981 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45459 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Kuhlencordt, KM A1 - Müller-Ruchholtz, W. T1 - Plating inhibition assay (PIA): a new test for cell mediated cytotoxicity N2 - No abstract available Y1 - 1979 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45443 ER - TY - JOUR A1 - Widder, A. A1 - Backhaus, J. A1 - Wierlemann, A. A1 - Hering, I. A1 - Flemming, S. A1 - Hankir, M. A1 - Germer, C.-T. A1 - Wiegering, A. A1 - Lock, J. F. A1 - König, S. A1 - Seyfried, F. T1 - Optimizing laparoscopic training efficacy by ’deconstruction into key steps’: a randomized controlled trial with novice medical students JF - Surgical Endoscopy N2 - Background Simulator training is an effective way of acquiring laparoscopic skills but there remains a need to optimize teaching methods to accelerate learning. We evaluated the effect of the mental exercise ‘deconstruction into key steps’ (DIKS) on the time required to acquire laparoscopic skills. Methods A randomized controlled trial with undergraduate medical students was implemented into a structured curricular laparoscopic training course. The intervention group (IG) was trained using the DIKS approach, while the control group (CG) underwent the standard course. Laparoscopic performance of all participants was video-recorded at baseline (t0), after the first session (t1) and after the second session (t2) nine days later. Two double-blinded raters assessed the videos. The Impact of potential covariates on performance (gender, age, prior laparoscopic experience, self-assessed motivation and self-assessed dexterity) was evaluated with a self-report questionnaire. Results Both the IG (n = 58) and the CG (n = 68) improved their performance after each training session (p < 0.001) but with notable differences between sessions. Whereas the CG significantly improved their performance from t0 –t1 (p < 0.05), DIKS shortened practical exercise time by 58% so that the IG outperformed the CG from t1 -t2, (p < 0.05). High self-assessed motivation and dexterity associated with significantly better performance (p < 0.05). Male participants demonstrated significantly higher overall performance (p < 0.05). Conclusion Mental exercises like DIKS can improve laparoscopic performance and shorten practice times. Given the limited exposure of surgical residents to simulator training, implementation of mental exercises like DIKS is highly recommended. Gender, self-assessed dexterity, and motivation all appreciably influence performance in laparoscopic training. KW - laparoscopic skills KW - teaching methods KW - deconstruction into key steps KW - laparoscopic course Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-323969 VL - 36 IS - 12 ER -