TY  - BOOK
A1  - Kossatz, Tilman
T1  - Johann Philipp Preuss (1605 - ca. 1687) : ein Beitrag zur Genese barocker Bildkunst in Franken
N2  - Diese Arbeit beschäftigt sich mit dem Würzburger Bildhauer und Architekten Johann Philipp Preuß (1605 - ca 1687).
KW  - Preiss, Hans Philipp
KW  - Franken
KW  - Plastik
KW  - Geschichte 1605-1687
KW  - Architekt
KW  - Bildhauer
KW  - Würzburg
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203117
ER  - 
TY  - JOUR
A1  - Hegmann, Joachim
A1  - Christl, Manfred
A1  - Peters, Karl
A1  - Peters, Eva-Maria
A1  - Schnering, Hans Georg von
T1  - Die Reaktionskaskade von 6-Oxo-5-phenyl-1,3,4-oxadiazin-2-carbonsäure-methylester und 1,3-Butadienen zu konjugierten und nichtkonjugierten Cyclopentenonen
N2  - Fünfgliedrige Carbocyclen sind Bauelemente zahlreicher NaturstofTe und daher attraktive Syntheseziele. Da bisher kein Syntheseverfahren mit großer Anwendungsbreite bekannt ist, sind neue Methoden willkommen. Wir berichten hier über Umsetzungen des Titelheterocyclus 1 mit l,3-Butadienen 1; diese Reaktionen, obwohl vielstufig, liefern im Eintopfverfahren konjugierte und nichtkonjugierte Cyclopentenone und gestatten auch die Fünfringanellierung.
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58421
ER  - 
TY  - JOUR
A1  - Adam, D.
A1  - Wittbrodt, J.
A1  - Telling, A.
A1  - Schartl, Manfred
T1  - RFLP for an EGF-receptor related gene associated with the melanoma oncogene locus of Xiphophorus maculatus
N2  - No abstract available
KW  - Physiologische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61822
ER  - 
TY  - JOUR
A1  - Schartl, Manfred
A1  - Peter, R. U.
T1  - Progressive growth of fish tumors after transplantation into thymus-aplastic (nu/nu) mice
N2  - No abstract available
KW  - Physiologische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61833
ER  - 
TY  - JOUR
A1  - Schartl, Manfred
T1  - A sex chromosomal restriction-fragment-length marker linked to melanoma-determining Tu loci in Xiphophorus
N2  - No abstract available
KW  - Physiologische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61842
ER  - 
TY  - JOUR
A1  - Gessler, Manfred
A1  - Bruns, Gail A. P.
T1  - Molecular mapping and cloning of the breakpoints of a chromosome 11p14.1-p13 deletion associated with the AGR syndrome
N2  - Chromosome 11p13 is frequently rearranged in individuals with the WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) or parts of this syndrome. To map the cytogenetic aberrations molecularly, we screened DNA from cell Unes with known WAGR-related chromosome abnormalities for rearrangements with pulsed fleld gel (PFG) analysis using probes deleted from one chromosome 11 homolog of a WAGR patient. The first alteration was detected in a cell line from an individual with aniridia, genitourinary anomalies, mental retardation, and a deletion described as 11p14.1-p13. We have located one breakpoint close to probe HU11-164B and we have cloned both breakpoint sites as well as the junctional fragment. The breakpoints subdivide current intervals on the genetic map, and the probes for both sides will serve as important additional markers for a long-range restriction map of this region. Further characterization and sequencing of the breakpoints may yield insight into the mechanisms by which these deletions occur.
KW  - Biochemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59264
ER  - 
TY  - JOUR
A1  - Engels, Bernd
A1  - Peyerimhoff, S.D.
T1  - Study of the 1s and 2s shell contributions to the isotropic hyperfine coupling constant in nitrogen
N2  - The istropic part of the hyperfine coupling constant is investigated by means of multireference configuration interaction calculations employing Gaussian basis sets. A detailed study of the 1s and 2s spin polarisation in the nitrogen atom and the NH molecule shows that the structure of the lower-energy space of the unoccupied orbitals is essential for the results. A contraction of the Gaussian basis is possible without loss of accuracy if enough flexibility is retained to describe the main features of the original space of unoccupied functions. Higher than double excitations are found to be non-negligible for the description of α\(_{iso}\).
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58793
ER  - 
TY  - JOUR
A1  - Engels, Bernd
A1  - Peyerimhoff, S.D.
A1  - Karna, S.P.
A1  - Grein, F.
T1  - The hyperfine coupling constants of the five lowest states of CH : An ab initio MRD-CI study
N2  - No abstract available
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58807
ER  - 
TY  - JOUR
A1  - Schneider, Wolfgang
T1  - Book Reviews: Cognition, Metacognition, and Reading
N2  - No abstract available
KW  - Psychologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62079
ER  - 
TY  - JOUR
A1  - Stopper, Helga
A1  - Zimmermann, U.
A1  - Neil, G. A.
T1  - Increased efficiency of transfection of murine hybridoma cells with DNA by electropermeabilization
N2  - Dispase-treated murine hybridoma cells (SP2/0-Ag14) were transfected with the G418 resistance gene bearing plasmid pSV2-neo by electropermeabilization with a high degree of efficiency. The cells were subjected to intermittent multiple high-voltage short duration (5 p.s) DC pulses at intervals of 1 min in a weakly conducting medium followed by selection in G418-containing medium. The transfection medium, temperature, pulse duration, and voltage were empirically determined by preliminary electropermeabilization experiments. Increasing the number of pulses resulted in a higher percentage of transfected cells, but a decrease in the number of viable cells, with the optimal transfectant yield resulting when five pulses of 10 kV jcm were administered. This method allows the rapid and efficient injection of DNA into mammalian cells, and permits the rapid production of stable, drug resistant hybridoma celllines for use in subsequent fusion experiments.
KW  - Toxikologie
KW  - DNA transfection
KW  - Electropermeabilization
KW  - Eukaryotic cell
KW  - Hybridoma
KW  - Drug resistance
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63488
ER  - 
TY  - JOUR
A1  - Feuerstein, G.
A1  - Letts, G.
A1  - Sirén, Anna-Leena
T1  - N-Ac-Leukotriene E\(_4\): Unique vascular activity in the conscious rat
N2  - No abstract available
KW  - Neurobiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63171
ER  - 
TY  - JOUR
A1  - Sirén, Anna-Leena
A1  - Lake, C. R.
A1  - Feuerstein, G.
T1  - Hemodynamic and neural mechanisms of action of thyrotropin releasing hormone in the rat
N2  - Tbe mechanisms mediating the etl'ects ofthyrotropin-releasing hormone (TRH) on the cardiovascular system were studied in the conscious rat. Intracerebroventricolar (i.c. v.) injection of TRH (8 pmol-80 nmollkg) induced dose-dependent lncreases in mean arterial pressure, heart rate, and cardiac index. Rindquarter blood Oow increased due to vasodilation, while an lncrease in renal and mesenteric vascular resistance caused a decrease in blood Oow in the respective organs. The plasma Ievels of norepinephrine a~d epinephrine were increased by TRH, while there was no change in plasma renin activity or vasopressin. Tbe cardiovascular actions of i.c. v. TRH were not in.fluenced by blockade of the renin-angiotensin system or vasopressin receptors. Tbe ganglion blocker chlorisondamine and the a 1- aod al-adrenoreceptor antagooist phentolamlne (2 mg/kg i.v.) abolished the increase in blood pressure and mesenteric vasoconstriction after i.c. v. TRH. Propranolol (2 mg/kg i. v.) blocked the TRH-ioduced increase in cardiac index, heart rate, and hindquarter blood flow. The hindquarter vasodllatlon lnduced by TRH was also blocked by the selective ß1-adrenocept9r antagonist ICI 188,551 (1 or 2 mg/kg i.v.), while tbe ,8,-adrenoceptor blocker practolol (10 mg/kg i.v.) had no eft'ect on the hindquarter vasodiJation produced by TRH but totally blocked the increase in cardiac Index. In adrenal demedullated rats, the systemic hemodynamic eft'ects ofi.c. v. TRH were dimlnished along with the decrease in renal blood flow and lncrease in renal vascular resistance; however, the iocrease in hfndquarter blood flow was attenuated only in adrenal demedullated rats pretreated with the sympathetlc blocker bretylium. The renal vasoconstriction induced by i.c. v. TRH was not abolished by renal denervation. In sinoaortic debufl'ered rats, the pressor, tachycardic, and mesenteric vasoconstrictor responses to centrally administered TRH were significantly potentiated. Taken together, these data soggest that the putative rieurotransmitter TRH may play a role in central regulation of cardiac functions and organ blood flow distribution through both tbe sympathetic nerves and the adrenal medulla. A pivotal roJe for ß1-adrenoceptors in mediation ofhindquarter vasodilation ls also demonstrated.
KW  - Neurobiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63183
ER  - 
TY  - JOUR
A1  - Sirén, Anna-Leena
A1  - Letts, G.
A1  - Feuerstein, G.
T1  - N-Acetyl-leukotriene E\(_4\) is a potent constrictor of rat mesenteric vessels
N2  - N-Acetyl-leukotriene E\(_4\) administered to conscious freely moving rats produced a dose-dependent vasoconstriction in the mesenteric vessels which led to profound reduction of blood flow to the gut. Renal and hindquarter blood flow and vascular resistance were not affected even by high doses of N-acetyl-leukotriene E\(_4\) . N-Acetyl-leukotriene E\(_4\) was 10-fold more potent than the thromboxane analog U-46619 and 1000-fold more potent than prostaglandin F\(_{2a}\) but 2-5-fold less potent than leukotriene D\(_4\)/E\(_4\) to induce mesenteric vasoconstriction. These data indicatc that N-acetylleukotriene E\(_4\) is a biologically active metabolite of peptide leukotrienes, and might play a role in cardiovascular derangements mediated by leukotrienes.
KW  - Neurobiologie
KW  - Peptide-leukotrienes
KW  - N-Acetyl-leukotriene E4
KW  - Prostaglandins
KW  - Mesenteric circulation
KW  - Anaphylactic shock
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63196
ER  - 
TY  - JOUR
A1  - Feuerstein, G.
A1  - Sirén, Anna-Leena
T1  - Mesenteric vascular responses to i.v. administration of lipoxin A\(_4\) and lipoxin B\(_4\) in the conscious rat
N2  - Lipoxin A (LXA\(_4\)) and lipoxin B\(_4\)(LXB\(_4\)) are newly discovered lipoxygenase-interacting products of leukocytes which might have a role in cardiovascular events associated with anaphylaxis. We have tested this possibility by systemic administration of both LXA\(_4\) and LXB\(_4\) to the conscious rat while monitaring systemic and regional hemodynamic changes. LXA\(_4\) and' LXB\(_4\) (l-100 pg/kg) produced dose-dependent constriction of the mesenteric vessels, up to + 123±23% and +50±9% for LXA\(_4\)/B\(_4\) , respectively. Dose-related changes were not observed in arterial blood pressure, heart rate, renal (LXB\(_4\)) and hindquarter blood ftow. We suggest that LXA\(_4\) and LXB\(_4\) might affect selective vascular beds, such as the mesenteric vessels, and contribute to variations in blood flow in specific pathophysiological states.
KW  - Neurobiologie
KW  - Lipoxin
KW  - Anaphylaxis
KW  - Mesenteric circulation
KW  - Renal circulation
KW  - Icosanoid
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63200
ER  - 
TY  - JOUR
A1  - Sirén, Anna-Leena
T1  - Cardiovascular pharmacology of thyrotropin releasing hormone
KW  - Neurobiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63214
ER  - 
TY  - JOUR
A1  - Feuerstein, G.
A1  - Sirén, Anna-Leena
T1  - Hypothalamic µ-receptors in the cardiovascular control: a review
N2  - The endogenous opioid system includes three major families of peptides [22): dynorphins (derived from pre-proenkephalin B); endorphins (derived from pre-proopiomelanocortin) and enkephalins (derived from pre-proenkephalin A). Multiple species of opioid peptides are derived from these major precursors and many of them possess potent cardiovascular properties. Multiple forms of opioid receptors have been defined in the central nervous system. Although the relationship of these receptors to the multiple actions of the opioid systems is not weil understood, some predications can be made: in vitro the dynorphin-related peptidesbind preferentially to kappa-opioid receptors; the enkephalins bind preferentially to delta and JL-opioid receptors and while beta-endorphin binds to mu- and delta-, but not to kappa-opioid receptors. While littleis known on the roJe ofthe opioid system in normal cardiovascular regulation, it has become clear that cardiovascular stress situations substantially modify the activity ofthe endogenous opioid system. This review focuses on the mu-opioid system in the hypothalamus with special emphasis on its potential roJe in cardiovascular control of both normal and pathophysiologic states.
KW  - Neurobiologie
KW  - µ opioid receptors
KW  - Hypothalamus
KW  - Cardiovascular system
KW  - Sympathetic nervous system
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63228
ER  - 
TY  - JOUR
A1  - Sirén, Anna-Leena
A1  - Feuerstein, G.
T1  - Cardiovascular effects of rat calcitonin gene-related peptide in the conscious rat
KW  - Neurobiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63236
ER  - 
TY  - JOUR
A1  - Hommers, Wilfried
T1  - Implicit psychological theories in legal thought on sentencing and liability
N2  - No abstract available
KW  - Haftung
KW  - Urteil
KW  - Verkündung
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-47093
ER  - 
TY  - JOUR
A1  - Schneider-Schaulies, Sibylle
A1  - Liebert, U. G.
A1  - Baczko, K.
A1  - ter Meulen, Volker
T1  - Molecular Biological Analyses of Measles Virus Gene Expression in the CNS of Acutely and Persistently Infected Rat Brain Cells
N2  - No abstract available
KW  - Masernvirus
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-34104
ER  - 
TY  - JOUR
A1  - Liebert, U. G.
A1  - Schneider-Schaulies, Sibylle
A1  - ter Meulen, V.
T1  - Measles Virus infections of the central nervous system (CNS) of rats
N2  - No abstract available
KW  - Masernvirus
KW  - Zentralnervensystem
KW  - Ratte
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-34087
ER  - 
TY  - BOOK
A1  - Kossatz, Tilman
T1  - Johann Philipp Preuss (1605 - ca. 1687) : ein Beitrag zur Genese barocker Bildkunst in Franken
N2  - Diese Arbeit beschäftigt sich mit dem Würzburger Bildhauer und Architekten Johann Philipp Preuß (1605 - ca 1687).
KW  - Preiss, Hans Philipp
KW  - Franken
KW  - Plastik
KW  - Geschichte 1605-1687
KW  - Architekt
KW  - Bildhauer
KW  - Würzburg
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-50645
N1  - Teilw. zugl.: Würzburg, Univ., Diss., 1983. 
Die vollständige Ausgabe liegt unter https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-203117 vor.
ER  - 
TY  - JOUR
A1  - Kekow, Jörn
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
A1  - Gross, Wolfgang L.
T1  - Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay
N2  - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IR! measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELlSA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELlSA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/mllRI (1 ng/ml - 23.4 JLU/ml - 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of :515%. Diabetes 37:321-26,1988
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45543
ER  - 
TY  - BOOK
T1  - Verantwortung in Umwelt und Schöpfung : eine Unterrichtsreihe für den Religionsunterricht der 10. Jahrgangsstufe an Gymnasien und Realschulen
N2  - No abstract available
KW  - Religionsunterricht
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-56873
ER  - 
TY  - JOUR
A1  - Schulze, Hans Joachim
A1  - Künzler, Jan
T1  - Lebenssituationen alter Menschen in der Stadt : Merkmale und Handlungsbedarf
N2  - No abstract available
KW  - Soziologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-51082
ER  - 
TY  - JOUR
A1  - Sponholz, Barbara
T1  - Beobachtungen zur Morphodynamik an Koris des südlichen Air-Vorlandes (Niger)
N2  - Am Beispiel von vier Koris l) (Téloua, Barghot, Tazolé, Oufaguédout) des südlichen Air-Vorlandes werden Beobachtungen zum aktuellen morphodynamischen Geschehen dieser Region vorgestellt. Durch die regionalen Unterschiede der durchschnittlichen jährlichen Niederschlagshöhen und durch unterschiedliche Charakteristika ihrer Einzugsgebiete können diese vier Koris als typische Vertreter verschiedener fluviatil/äolischer Formungsgruppen angesehen werden. Der Übergang von vorherrschend fluviatiler zu vorherrschend äolischer Formung liegt dabei im Bereich des Kori Oufaguédout, im östlichen Teil des Untersuchungsraumes. Hier konnte durch mehrere Schürfe auch der zeitliche Übergang zwischen beiden Formungskreisen in der jüngeren Reliefgeschichte nachgewiesen werden.
N2  - Four koris (Téloua, Barghot, Tazolé, Oufaguédout) in the southern foreland of the Air-Mountains were studied regarding their recent morphological dynamics. Caused by regional differences in mean annual rainfall and the different characteristics of their drainage areas each of these koris represents one group of typical fluvial/eolian influence complex. The transition between fluvial and eolian processes is located in the area of the kori Oufaguédout, in the eastern part of the study area. Also the transition in time between the both processes during younger relief formation periods was proven there.
KW  - Geographie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-53579
ER  - 
TY  - JOUR
A1  - Hallenbeck, JM
A1  - Dutka, AJ
A1  - Kochanek, PM
A1  - Sirén, Anna-Leena
A1  - Pezeskpour, GH
A1  - Feuerstein, G.
T1  - Stroke risk factors prepare rat brainstem tissues for a modified localized Shwartzman reaction
N2  - Stroke risk factors such as hypertension, diabetes, advanced age, and genetic predisposition to stroke were demonstrated to prepare rat brainstem tissues for a modified local Shwartzman reaction. A single intracisternal injection of endotoxin provoked the reaction, and affected rats manifested neurologie deficits accompanied by pathologie lesions. Brainstem infarcts developed in only a small proportion of rats without recognized risk factors after intracisternal injection of endotoxin. Thus, stroke risk factors, whieh are ordinarily regarded as operating through acceleration of atherosclerosis, may predispose to brain ischemia by local effects on brain mierocirculation such as those thought to underlie preparation of a tissue for the local Shwartzman reaction.
KW  - Gehirn
KW  - Durchblutung
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-47971
ER  - 
TY  - JOUR
A1  - Busche, Detlef
A1  - Sponholz, Barbara
T1  - Karsterscheinungen in nichtkarbonatischen Gesteinen der Republik Niger
N2  - Vorgestellt werden Lösungsformen in karbonatfreien Sandsteinen und Eisenkrusten der östlichen Republik Niger. Diese Formen haben eine außerordentlich weite Verbreitung und sind wesentliche Elemente der Landschaft. Beschrieben werden sowohl auf den Dachflächen der südsaharisch/sahelischen Schichtstufen entwickelte Depressionen mit subterraner Entwässerung als auch an Wadi hängen und steilen Stufenabschnitten gekappte, horizontal bis schräg verlaufende Höhlen und Röhren. Viele dieser Lösungsformen sind zumindest in Resten noch von einer Eisen- oder Kieselkruste ausgekleidet, die u.a. als Indiz für eine Entstehung der Formen im Grundwasserbereich angesehen werden kann. Die Anlage der Karstformen wäre demnach noch vor die Herauspräparierung der Stufen und Inselberge im ausgehenden Tertiär zu stellen. Die Karstformen in nichtkarbonatischen Gesteinen gleichen bis ins Detail denen des Karbonatkarstes. Eine initiale Formung durch äolische oder fluviatile Prozesse oder durch Piping kann ausgeschlossen werden.
N2  - The authors describe subaerial and subterranean solution features in noncarbonatic sandstones and iron crusts from eastern Niger. They are of common occurence and are an important element of the landscape. The paper discusses karst depressions of various sizes on all the plateau surfaces of the saharo-sahelian scarpland and mesa landscape, together with their subterranean network of tubes and caves. In some tubes relicts of an iron or silica coating are preserved, one evidence among other, morphological ones that the passageways developed when completely filled with groundwater. The system originated during the Miocene and became dismembered during Pliocene scarpland and inselberg formation. The noncarbonatic karst forms closely resemble those of carbonate karst. Aeolian and fluvial processes, including piping, can be excluded as having created these landforms.
KW  - Geographie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-55858
ER  - 
TY  - JOUR
A1  - Moll, Heidrun
A1  - Bösing-Schneider, Rita
T1  - The transplantation barrier of nude mice
N2  - Syngeneic memory cells can be stimulated to yield a secondary immune response after their transfer into irradiated euthymie recipients as well as into young thymusless nude mice. It is shown that nude mice older than twelve weeks of age are not permissive towards memory cell activation as it is found in non-irradiated euthymie animals. This barrier to isogeneie or congeneic cells seems to be caused by a pool of cyclophosphamide-sensitive cells. Since young nude mice could be rendered as unpermissive as older nude mice by pretreatment with either PNA-agglutinable thymus cells or nylon-wool passed spleen cells, it is suggested that an increased number of precursor T cells in older nude mice might induce this effect. Further experiments with monoclonal antibodies against the Lyt-l, Lyt-2, and L3T4 marker on T cells indicate that T -helper/inducer activity might be required to establish the "isogeneie barrief" in nude mice.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46045
ER  - 
TY  - JOUR
A1  - Hommers, Wilfried
T1  - Review of "Roskam, E.E., & Suck, R. (Eds.): Progress in mathematical psychology. Amsterdam: North-Holland, 1987, pp. 538."
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43525
ER  - 
TY  - JOUR
A1  - Schenk, Wolfdieter A.
A1  - Kuemmerle, Dagmar
A1  - Burschka, Christian
T1  - Die Koordinationschemie C=S-funktioneller Verbindungen: VIII. Addition aktivierter Alkine an Wolfram-CS\(_2\)-Komplexe und Aufbau tetraedrischer Methylidindicobaltwolfram-Cluster
N2  - No abstract available
KW  - Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46676
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Expression of MHC Structures on Immunologically Reactive and Non Reactive Cells in Islets of Langerhans and Other Tissues
N2  - No abstract available
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45557
ER  - 
TY  - JOUR
A1  - Zvirblis, G. S.
A1  - Gorboulev, Valentin G.
A1  - Rubtsov, P. M.
A1  - Chernov, B. K.
A1  - Golova, Yu. B.
A1  - Pozmogova, G. E.
A1  - Skryabin, K. G.
A1  - Bayev, A. A.
T1  - Genetic engineering of peptide hormones : III. Cloning of cDNA of porcine growth hormone and construction of gene for expression of hormone in bacteria
N2  - Results are presented of cloning cDNA of procine growth hormone, analysis of its primary structure, and creation of a construction capable of expression of this cDNA in Esqheriahia coti cells. It is shown that in the population of mRNA coding porcine growth hormone, heterogeneity is noted which is manifested not only at the level of the nucleotide sequence, but also is reflected in the amino acid sequence of the mature hormone.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46958
ER  - 
TY  - JOUR
A1  - Rubtsov, P. M.
A1  - Oganessyan, R. G.
A1  - Gorboulev, Valentin G.
A1  - Skryabin, K. G.
A1  - Bayev, A. A.
T1  - Genetic engineering of peptide hormones : II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning
N2  - Primary structure is determined of an insertion of a clone isolated from the library of hypophyseal cDNA of cattle by hybridization with a probe specific for prolactin. Analysis of nucleotide sequences showed that in the process of cloning, reorganization occurred in structure of preprolactin cDNA, including an inversion of the 5'-terminal and deletion of the central section of cDNA. Nevertheless, from structure of cDNA, nucleotide sequences can be deduced of extended 5'- and 3'-terminal sections of preprolactin mRNA in cattle with lengths of 257 and 551 nucleotide residues, respectively. When these sequences are compared to those established previously, some differences were found in primary structure. The most important of them is the presence of an additional codon which codes alanine at the position (-22) of the signal peptide. It is suggested that heterogeneity of preprolactin mRNA of cattle in the section coding the signal peptide is the result of alternative splicing, as was shown for preprolactin mRNA in rats.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46975
ER  - 
TY  - JOUR
A1  - Sagelsdorff, P.
A1  - Lutz, Werner K.
A1  - Schlatter, C.
T1  - DNA methylation in rat liver by daminozide, 1,1-dimethylhydrazine, and dimethylnitrosamine
N2  - DNA Methylation in Rat Li ver by Daminozide, 1, 1-Dimethylhydrazine, and Dimethylnitrosamine. SAGELSDORFF, P., LUTZ, W. K., AND ScHLAITER C. (1988). Fundam. Appl. Toxico/. 11, 723-730. [methyP4C]Daminozide (succinic acid 2',2'-dimethylhydrazide; 37 mgjkg), l,l( 14C]dimethylhydrazine (UDMH; 19 mgtkg), and (14C]dimethylnitrosamine (DMNA; 0.1 mg/ kg) were administered by oral gavage to male Sprague-Dawley rats. After 24 hr, the animals were killed and DNA was purified from the livers to constant specific radioactivity. After enzymatic degradation of the DNA to the 3'-deoxynucleotides the Ievel of DNA methylation was determined by HPLC analysis. Radiolabeled 7-methylguanine (7mG) was identified by cochromatography with unlabeled 7mG added as standard after acidic depurination of DNA and HPLC analysis ofpurines and apurinic acid. All three compounds were found to methylate DNA. The relative potencies were 1:47:4900 for daminozide:UDMH:DMNA. With [methyPH]UDMH, the formation of7mG was investigated as a function of dose administered, at 20, 2, and 0.2 mgj kg. The methylation ofDNA was strictly proportional to the dose. The data were used to compare the Ievel of DNA alkylation derived from residues of daminozide and UDMH in treated apple with the genotoxicity of the intake of N-nitroso compounds in Germany and Japan. It is estimated that these residues could Iead to a DNA methylation in the Ii ver of about 6% of an average exposure to DMNA
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60875
ER  - 
TY  - JOUR
A1  - Lohse, M. J.
A1  - Elger, B.
A1  - Lindenborn-Fotinos, J.
A1  - Klotz, Karl-Norbert
A1  - Schwabe, U.
T1  - Separation of solubilized A\(_2\) adenosine receptors of human platelets from non-receptor [\(^3\)H]NECA binding sites by gel filtration
N2  - Human platelet membranes were solubilized with the zwitterionic detergent CHAPS (3-[3-(cholamidopropyl)dimethylammonio]- 1-propanesulfonate) and the solubilized extract subjected to gel ftltration. Binding of the adenosine receptor agonist [\(^3\)H]NECA (5'-N-ethylcarboxamidoadenosine) was measured to the eluted fractions. Two [\(^3\)H]NECA binding peaks were eluted, the first of them with the void volume. This first peak represented between 10% and 25% of the [\(^3\)H]NECA binding activity eluted from the column. It bound [\(^3\)H]NECA in a reversible, saturable and GTPdependent manner with an affinity of 46 nmol/1 and a binding capacity of 510 fmol/mg protein. Various adenosine receptor ligands competed for the binding of [\(^3\)H]NECA to the frrst peak with a pharmacological proftle characteristic for the A\(_2\) adenosine receptor as determined from adenylate cyclase experiments. In contrast, most adenosine receptor ligands did not compete for [\(^3\)H]NECA binding to the second, major peak. These results suggest that a solubilized A\(_2\) receptor-Gs protein complex of human platelets can be separated from other [\(^3\)H]NECA binding sites by gel filtration. This allows reliable radioligand binding studies of the A2 adenosine receptor of human plate1ets.
KW  - Toxikologie
KW  - A<sub>2</sub> Adenosine receptor
KW  - Human platelets
KW  - Radioligand binding
KW  - Adenylate cyclase
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60309
ER  - 
TY  - JOUR
A1  - Cristalli, G.
A1  - Franchetti, P.
A1  - Grifantini, M.
A1  - Vittori, S.
A1  - Klotz, Karl-Norbert
A1  - Lohse, M. J.
T1  - Adenosine receptor agonists: Synthesis and biological evaluation of 1-deaza analogues of adenosine
N2  - In a search for more selective A\(_1\) adenosine receptor agonists, N\(^6\)-[(R)-(-)-1-methyl-2-phenethyl]-1-deazaadenosine (1-deaza-R-PIA, 3a), N\(^6\)-cyclopentyl-1-deazaadenosine (1-deazaCPA, 3b), N\(^6\)-cyclohexyl-l-deazaadenosine (1-deazaCHA, Sc), and the corresponding 2-chloro derivatives 2a-c were synthesized from 5,7-dichloro-3-ß-D-ribofuranosyl-3Himidazo[ 4,5-b]pyridine (1). On the other band, N-ethyl-1'-deoxy-1'-(1-deaza-6-amino-9H-purin-9-yl)-ß-D-ribofuranuronamide (1-deazaNECA, 10) was prepared from 7-nitro-3-ß-D-ribofuranosyl-3H-imidazo[4,5-b]pyridine (4), in an attempt to find a more selective A\(_2\) agonist. The activity of all deaza analogues at adenosine receptors has been determined in adenylate cyclase andin radioligand binding studies. 1-DeazaNECA (10) proved tobe a nonselective agonist at both subtypes of the adenosine receptor. It is about 10-fold less active than NECA but clearly more active than the parent compound 1-deazaadenosine as an inhibitor of platelet aggregation and as a stimulator of cyclic AMP accumulation. The N\(^6\)-substituted 1-deazaadenosines largely retain the A\(_1\) agonist activity of their parent compounds, but lose some of their A\(_2\) agonist activity. This results in A\(_1\)-selective compounds, of which N\(^6\)cyclopentyl- 2-chloro-1-deazaadenosine (1-deaza-2-Cl-CPA, 2b) was identified as the most selective agonist at A\(_1\) adenosine receptors so far known. The activity of all 1-deaza analogues confirms that the presence of the nitrogen atom at position 1 of the purine ring is not critical for A\(_1\) receptor mediated adenosine actions.
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60262
ER  - 
TY  - JOUR
A1  - Lohse, M. J.
A1  - Klotz, Karl-Norbert
A1  - Schwabe, U.
A1  - Cristalli, G.
A1  - Vittori, S.
A1  - Grifantini, M.
T1  - 2-Chloro-N\(^6\)-cyclopentyladenosine: a highly selective agonist at A\(_1\) adenosine receptors
N2  - 2-Chloro-N\(^6\)-cyclopentyladenosine (CCPA) was synthesized as a potential high affinity ligand for At adenosine receptors. Binding of [\(^3\)H]PIA to A1 receptors of rat brain membranes was inhibited by CCP A with a Ki-value of 0.4 nM, compared to a Ki-value of 0.8 nM for the parent compound N\(^6\)-cyclopentyladenosine (CPA). Binding of [\(^3\)H]NECA to A\(_2\) receptors of rat striatal membranes was inhibited with a Ki-value of 3900 nM, demonstrating an almost 10,000-fold A\(_1\)-selectivity of CCPA. CCP A inhibited the activity of rat fat cell membrane adenylate cyclase, a model for the A\(_1\) receptor, with an IC\(_{50}\)-value of 33 nM, and it stimulated the adenylate cyclase activity of human platelet membranes with an EC\(_{50}\)-value of 3500 nM. The more than 100-fold A\(_1\)-selectivity compares favourably with a 38-fold selectivity of CPA. Thus, CCPA is an agonist at A\(_1\) adenosine receptors with a 4-fold higher selectivity and 2-fold higher affinity than CPA, and a considerably higher selectivity than the standard At receptor agonist R-N\(^6\) -phenylisopropyladenosine (R-PIA). CCP A represents the agonist with the highest selectivity for A\(_1\) receptors reported so far.
KW  - Toxikologie
KW  - Adenosine receptors
KW  - Adenylate cyclase
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60279
ER  - 
TY  - JOUR
A1  - Lohse, M. J.
A1  - Klotz, Karl-Norbert
A1  - Diekmann, E.
A1  - Friedrich, K.
A1  - Schwabe, U.
T1  - 2',3'-Dideoxy-N\(^6\)-cyclohexyladenosine: an adenosine derivative with antagonist properties at adenosine receptors
N2  - Tbe 2',3'-dideoxy analogue of the potent A\(_1\) receptor agonist, N\(^6\)-cyclohexyladenosine (CHA), was synthesized as a potential antagonist for the A\(_1\) adenosine receptor. In sturlies on adenylate cyclase 2',3'-dideoxy-N\(^6\)-cyclohexyladenosine (ddCHA) did not show agonist properties at A\(_1\) or at A\(_2\) receptors. However, it antagonized the inhibition by R-PIA of adenylate cyclase activity of fat cell membranes via A\(_1\) receptors with a K\(_i\) value of 13 \(\mu\)M. ddCHA competed for the binding of the selective A1 receptor antagonist, [\(^3\) HJ8-cyclopentyl-1,3-dipropylxantbine ([\(^3\)H]DPCPX), to rat brain membranes with a K\(_i\) value of 4.8 \(\mu\)M; GTP did not affect the competition curve. In contrast to the marked stereoselectivity of the A\(_1\) receptor for the cx- and the natural ß-anomer of adenosine, the cx-anomer of ddCHA showed a comparable affinity for the A\(_1\) receptor (K\(_i\) value 13.9 \8\mu\)M). These data indicate that the 2'- and 3'-hydroxy groups of adenosine and its derivatives are required foragonist activity at and high affinity binding to A\(_1\) adenosine receptors and for the distinction between the cx- and ß-forms.
KW  - Toxikologie
KW  - Adenosine receptors
KW  - Adenylate cyclase
KW  - Adenosine receptor antagonists
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60282
ER  - 
TY  - JOUR
A1  - Klotz, Karl-Norbert
A1  - Lohse, M. J.
A1  - Schwabe, U.
T1  - Chemical modification of A\(_1\) adenosine receptors in rat brain membranes - evidence for histidine in different domains of the ligand binding site
N2  - Chemical modification of amino acid residues was used to probe the ligand recognition site of A\(_1\) adenosine receptors from rat brain membranes. The effect of treatment with group·specific reagents on agonist and antagonist radioligand binding was investigated. The histidine-specific reagent diethylpyrocarbonate (DEP) induced a loss of binding of the agonist R-N\(^6\)-[\(^3\)H]phenylisopropyladenosine ([\(^3\)H]PIA), which could be prevented in part by agonists, but not by antagonists. DEP treatment induced also a loss of binding of the antagonist [\(^3\)H]8- cyclopentyl-1 ,3-dipropylxanthine ([\(^3\)H]DPCPX). Antagonists protected A\(_1\) receptors from this inactivation while agonists did not. This result provided evidence for the existence of at least 2 different histidine residues involved in ligand binding. Consistent with a modification of the binding site, DEP did not alter the affinity of [\(^3\)H]DPCPX, but reduced receptor number. From the selective protection of [\(^3\)H] PIA and [\(^3\)H]DPCPX binding from inactivation, it is concluded that agonists and antagonists oocupy different domains at the binding site. Sulfhydryl modifying reagents did not influence antagonist binding, but inhibited agonist binding. This effect is explained by modification of tbe inhibitory guanine nucleotide binding protein. Pyridoxal 5-phosphate inactivated both [\(^3\)H]PIA and [\(^3\)H]DPCPX binding, but the receptors could not be protected from inactivation by ligands. Therefore, no amino group seems to be located at the Iigand binding site. In addition, it was shown that no further amino acids witb polar side chains are present. The absence of bydrophilic amino acids frout the recognition site of the receptor apart from histidine suggests an explanation for the lack of hydrophilic ligands with high affinity for A\(_1\) receptors.
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60295
ER  - 
TY  - JOUR
A1  - Lutz, Werner K.
A1  - Maier, P.
T1  - Genotoxic and epigenetic chemical carcinogenesis: one process, different mechanisms
N2  - Chemieals that induce cancer in an intact organism are called carcinogens. This term does not differentiale between their various modes of action. In this review, Werner Lutz and Peter Maier make a mechanistic distinction between carcinogens that alter the genetic information and carcinogens that interfere with epigenetic processes. They considercardnogenesis tobe an ongoing, part1y unavoidable process which is based on a succession of mutations, most likely in stem cells, leading to autonomaus cellular growth regulation. Chemical carcinogens either induce such changes through mutations (genotoxic carcinogens) or they aceeierate the accumulation of critica1 spontaneaus mut11tions (epigenetic carcinogens). Examples are given for both classes of carcinogens, and for the processes that act at genoto:tic/nuclear 11nd epigenetic/mitotic Ievels.
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60884
ER  - 
TY  - JOUR
A1  - Lutz, Werner K.
A1  - Deuber, R.
A1  - Caviezel, M.
A1  - Sagelsdorff, P.
A1  - Friederich, U.
A1  - Schlatter, C.
T1  - Trenbolone growth promotant: covalent DNA binding in rat liver and in Salmonella typhimurium, and mutagenicity in the Ames test
N2  - DNA binding in vivo: (6,7-\(^3\)H]ß-trenbolone (ß-TBOH) was administered p.o. and i.p. to rats. After 8 or 16 h, DNA was isolated from the livers and purified to constant specific radioactivity. Enzymatic digestion to deoxyribonucleotides and separation by HPLC revealed about 90% ofthe DNA radioactivity eluting in the form of possible TBOH-nucleotide adducts. The extent of this genotoxicity, expressed in units of the Covalent Binding Index, CBI = (~mol TBOH bound per mol nucleotide)/(mmol TBOH administered per kg body weight) spanned from 8 t~ 17, i. e. was in the range found with weak genotoxic carcmogens. Ames test: low doses of ß-TBOH increased the number of revertants in Salmonella strain TAl 00 reproducibly and m a dose-dependent manner. The mutagenic potency was 0.2 revertants per nmol after preincubation of the bacteria (20 min at 37° C) with doses between 30 and 60 \(\mu\)g per plate (47 and 94 \(\mu\)g/ml preincubation mixture). Above this dose, the number of revertants decreased to control values, accompanied by a reduction in survival. The addition of rat liver S9 inhibited the mutagenicity. DNA binding in vitro: calf thymus DNA was incubated with tritiated ß-TBOH with and without rat liver S9 Highest DNA radioactivities were determined in the absence of the "activation" system. Addition of inactive S9 (without cofactors) reduced the DNA binding by a factor of up to 20. Intermediate results were found with active S9. DNA binding in Salmonella: ß-TBOH was irreversibly bound to DNA isolated from S. typhimurium TA100 after incubation of bacteria with [\(^3\)H]ß-TBOH. Conclusions: Covalent DNA binding appears to be the mechanism of an activation-independent ("direct") mutagenicity of TBOH which is not easily detected because of the bactericidal activity. The genotoxicity risk arising from exposure of humans to trenbolone residues in meat was estimated using the in vivo data and compared to that from the exposure to unavoidable genotoxins aflatoxin B1 and dimethylnitrosamine. It ts concluded that trenbolone residues represent only a low genotoxic risk.
KW  - Toxikologie
KW  - Trenbolone
KW  - Anabolieagent
KW  - DNA binding
KW  - Genotoxicity
KW  - Ames test
KW  - Salmonella typhimurium
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60897
ER  - 
TY  - JOUR
A1  - Schneider, Wolfgang
A1  - Sodian, Beate
T1  - Metamemory-memory behavior relationships in young children: Evidence from a memory-for-location task
N2  - No abstract available
KW  - Psychologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62062
ER  - 
TY  - JOUR
A1  - Parkkinen, J.
A1  - Korhonen, T. K.
A1  - Pere, A.
A1  - Hacker, Jörg
A1  - Soinila, S.
T1  - Binding sites in the rat brain for Escherichia coli S fimbriae associated with neontal meningitis
N2  - Escherichia coli strains that cause sepsis and meningitis in neonatal infants carry S fimbriae that bind to sialyl galactoside units of cell surface glycoproteins. To investigate the possible role of S fimbriae in determining the tissue tropism of neonatal menlngitis, we have studied the preselice of binding sites for S fimbriae in different tissues of the neonatal rat which is susceptible to meningitis caused by S-fimbriated E. coli. Purified S fimbriae were incubated on cryostat sections of different rat oipns and their bindina was assessed by indirect immunofluorescence. In the bnin of the neonatal rat, S fimbriae specifically bound to the luminal surfaces of the vascular endothelium and of the epithelium lining the choroid plexuses and bnin ventricles. The · bindlog W.s completely inhibited by the trisaccharide NeuAca2-3Ga)ßl-4Gic, a receptor analogue of S fimbriae, and by a preceding neuraminidase treatment of the sections. A recombinant E. coli strain expressina S fimbriae adhered in large numbers to the same tissue sites in the neonatal brain sections as did the purified fimbriae, · whereas the nonfimbriated host strahi and a recombiiuuit strain expresslog P fi.mbriae did not adhere to brain tissues. The results soggest that adhesion of S-fimbriated bacteria to the binding sites observed in the neonatai bnin has a pathogenetic roJe durlog bacterial Invasion from cii'culation into the cerebrospinal fluid.
KW  - Infektionsbiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59500
ER  - 
TY  - JOUR
A1  - Ott, M.
A1  - Hoschützky, H.
A1  - Jann, K.
A1  - Van Die, I.
A1  - Hacker, Jörg
T1  - Gene clusters for S fimbrial adhesin (sfa) and F1C Fimbriae (foc) of Escherichia coli: Comparative aspects of structure and function
N2  - Fimbrial 8dhesins en8ble b8cteria to 8ttach t9 eucaryotic ceU~. The genetic determin8nts for S fimbrial 8dhesins (sja) an.d for FlC ("pseudotype I") fimbri8e ifoc) were compared. Sfa and FlC represent functionally distinct 8dbesins in tbeir receptor specificities. Nevertheless, 8 high degree of bomology between both determin8nts was found on the basis of DNA-DNA hybridizations. Characteristic difl'erences in the restriCtion maps of tbe corresponding gene clusters, bowever, were visible in regions coding for the fimbrial subunits and for the S-specific 8dhesin. While a plasmid carrying the geneiic deternlinant for FlC fimbri8e was 8ble to complement transposon-induced sfa mutants, 8 plasmid carrying tbe genetic determin8nt for 8 tbird 8dht$in type, termed P fimbriae, was un8ble to do so. Proximal sfa-specific sequences carrying the S fimbrial st'"uctural gene were fused to sequences representing tbe di$tal part of the foc gene cluster to form 8 hybrid cluster, and tbe foc proxim~ region coding for tbe structural protein was Iigated to sfa distal sequences to form 8 second hybrid. Botb hybrid clones produced intact fimbriae. Anti-FlC monoclonal8ntibodies (MAbs) only recognized clones which produced FlC fimbriae, and an ~ti-S 8dhesin MAb marked clones whicb expressed the S adhesin. Bowever, one of four other anti-S fimbri8e-specific MAbs reacted witb both fimbrial structures, S and FlC, indicating 8 common epitope on both antigens. The results presented bere ~upport tbe view th8t sfa and foc determinants code for fimbri8e tb8t 8re simil8r in several aspects, wbile the P fimbri8e are members of 8 more distantly rel8ted group.
KW  - Infektionsbiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59519
ER  - 
TY  - JOUR
A1  - Pawelzik, M.
A1  - Heesemann, J.
A1  - Hacker, Jörg
A1  - Opferkuch, W.
T1  - Cloning and characterization of a new type of fimbria (S/F1C-related fimbria) expressed by an Escherichia coli O75:K1:H7 blood culture isolate
N2  - The Escherichia coli blood culture isolate BK658 (07S:K1:H7) expresses F1A and F1B fimbriae as weil as a third fimbrial type which reacts with anti-S-fimbrial antiserum but fails to show S-specific binding properlies (i.e., agglutination of bovine erythrocytes). To characterize these fimbriae, we cloned the respective genetic determinant in E. coli K-12. The resulting recombinant clone HB101(pMMP658-6) expresses fimbriae of 1.2-p.m length and a diameter of approximately 7 nm. The determinant codes for the fimbrillin subunit, a protein of 17 kUodaltons in size, and for at least five other proteins of 87, 31, 23, 14.3, and 13.8 kUodaltons. By restriction analysis and by DNA-DNA hybridization, it could be shown that the cloned fimbrial determinant of strain BK658 exhibits a high degree of sequence homology to the gene clusters coding for S fimbrial adhesins (sfa) and F1C fimbriae (/oc). By using the Western blot (immunoblot) technique and a quantitative enzyme-linked immunosorbent assay, it could be further demonstrated that the cloned fimbriae of BK658, S fimbriae, and FlC fimbriae share cross-reactive epitopes as weil as antigenic determinants specific for each fimbrial type. No antigenic cross-reactivity with F1C fimbriae could be detected. The results indicate a genetical and serological relatedness of the cloned fimbriae toS fimbriae and F1C fimbriae. Therefore, this new type of fimbriae is preliminarily termed SIF1C-related fimbriae (Sfr).
KW  - Infektionsbiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59529
ER  - 
TY  - JOUR
A1  - Munoa, F.
A1  - Hacker, Jörg
A1  - Juarez, A.
T1  - Characterization of a chromosomal mutant that blocks hemolysin excretion in Escherichia coli
N2  - We analyzed an Escherichia coli strain which harbours a chromosomal mutation that blocks the hemolysin excretion. Compartmentation studies showed that hemolysin accumulates in the cytoplasm and not in the periplasm. The mutation did not affect the SDS-PAGE protein pattern of the outer membrane, although some alterations were apparent in the periplasmic protein pattern. The mutant strain, E. coli Hsb-1 also failed to export a cloned fimbrial adhesin. The mutation maps in the min. 3.5 of the E. coli genetic map.
KW  - Infektionsbiologie
KW  - Hemolysin excretion
KW  - Escherichia coli
KW  - Mutant
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59534
ER  - 
TY  - JOUR
A1  - Freund, S.
A1  - Henneberger, H.
A1  - Christl, Manfred
T1  - Halbkäfigverbindungen aus Diels-Alder-Addukten des Benzvalens mit Cyclopentadien-Derivaten durch Radikalreaktionen mit Thiophenol und Bromtrichlormethan
T1  - Half-Cage Compounds by Radical Reactions with Thiophenol and Bromotrichloromethane from Diels-Alder Adducts of Benzvalene with Cyclopentadiene Derivatives
N2  - In Diels-Alder-Reaktionen wurden aus Benzvalen (1) und Tetrachlorcyclopentadienon- dimethylacetal sowie Tetrachlorfuran die Addukte 2 bzw. 8 und 9 erhalten. Behandlung von 2 mit Natrium und tert-Butylalkohol ergab das chlorfreie Acetal 3, dessen Doppelbindung sich mit Diimin unter Bildung von 4 absättigen ließ. Aus dem bekannten Stammkohlenwasserstoff 5 ging analog 6 hervor, und durch Reaktion seines Anions mit Chlorameisensäure- methylester entstand der Ester 7. - Die Verbindungen l, 3, 5, 7-9 und das bekannte U wurden mit Thiophenol so behandelt, daß Radikalkettenreaktionen abliefen. Bei 8 trat Addition an die Zentralbindung des Bicyclobutan-Systems ein. Dagegen unterlag bei 2, 9, U und zum Teil bei 3, deren Doppelbindung jeweils der BicycJobutan-Einheit benachbart ist, das Primärradikal der 5-Hexen-1-yl- Cyclopentylrneth.yl-Umlagerung, aus der letztlich 15, ll, 13 bzw. 16 resultierten. Zum Teil bei 3 und vollständig bei 5 erfolgte die Addition an die Doppelbindung zu 17 bzw. 18, d.h. ohne Beteiligung der benachbarten Bicyclobutan- Einheit. Durch Aktivierung des Bicyclobutan-Systems mit einer Esterfunktion konnte dessen Passivität aufgehoben werden. So entstanden aus 7 das nichtumgelagerte Produkt 19 und das umgelagerte 20. Ihr Verhältnis hing von der Konzentration an Thiophenol ab. - Analog zur Reaktion 1Z + Thiophenol-13 brachte BromtrichJormethan aus 12 das umlagerte Addukt 21 hervor. - Als Nebenprodukt wurde bei der Umsetzung von 12 mit Thiophenol das Chlorwasserstoff-Addukt 14 des ersteren gefunden. Modellreaktionen für die Bildung von 14 sind die Umsetzungen von 12 mit Essigsäure und von 3 sowie 5 mit +Nitrobenzoesäure zu den Addukten 22, 23 bzw. 24. Seit
N2  - The adducts 2, 8,. and 9 were obtained by Diels-Aider reactions of benzvalene (I) with tetrachlorocyclopentadienone dimethyl acetal and tetracblorofuran. respectively. On treatment of 2 with sodium and tert-butyl alcohol, the chlorine atoms were substituted by hydrogen atoms to give acetal 3, the double bond of · whicb could be hydrogenated by diimine with formation of 4. Analogously, the known parent hydrocarbon 5 afforded 6. Tbe anion of 5 reacted with methyl chloroformate to give the ester 7. - Compounds 2, 3, 5, 7-9, and the known 12 were treated with thiophenol under conditions causing radical chain reactions. Whereas 8 added the reagent across the centrat bond of the bicyclobutane system, the additions to 2, 9, 12 and in part to 3, the double bond of which is adjaceot to the bicyclobutane moiety in each case, were accompanied by a 5-hexen-1-yJcylcopentylmethyl rearrangement of the primary radical resulting in formation of 15, 11, 13, and 16, respectively. In part with 3 and completely with 5, the addition took place at the double bond to give 17 and 18, respectively, i.e. without involvement of the adjacent bicylcobutane moiety. Activation of the bicyclobutane system with an ester functionality led to its participation. Thus, compound 7 gave the nonrearranged product 19 and the rearranged one 20 with their ratio being dependent on the concentration of thiophenol. - In analogy to the reaction 12 + thiophenol-13, tbe rearranged adduct 21 was formed from 12 and bromotrichloromethane. - The hydrogen cbloride adduct 14 was found as a byproduct on treatment of 1l witb thiophenol. Model reactions for the formation of 14 are the additions of acetic acid to 12 an~ 4-nitrobenzoic acid to 3 and 5 leading to the adducts 22, 23, and 24, respectively.
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58435
ER  - 
TY  - JOUR
A1  - Christl, Manfred
A1  - Henneberger, H.
A1  - Freund, S.
T1  - Halbkäfigverbindungen aus Polycyclen mit einem Bicyclo[1.1.0]butan-System und dazu benachbarter Azobrücke durch Radikalreaktionen mit Thiophenol und Bromtrichlormethan
T1  - Half-Cage Compounds by Radical Reactions with Thiophenol and Bromotrchloromethane from Polycyclic Compounds with a Bicyclo[1.1.0]butane System and an Adjacent Azo Bridge
N2  - Aus Benzvalen (J) und 3,6-Bis(2-pyridyl)-l,2,4,5-tetrazin wurde das Dihydropyridazin 2c erhalten, das ebenso wie die bekannten Dihydropyridazine 2a, b mit Cyclopropen in die entsprechende Azoverbindung 3 überging. Addition von Thiophenol an 3a, c und 5 lieferte unter Beteiligung des Bicyclobutan-Systems und der Azofunktion die Halbkäfigverbindungen 4a, c bzw. 6. Aus der Umsetzung von 7, in dem die Azobrücke durch zwei BicycJobutan- Systeme flankiert ist, mit Thiophenol sowie Bromtrichlormethan gingen die Halbkäfigverbindungen 8 bzw. 9 hervor. An diesen ~eaktionen wirken beide Bicyclobutan-Systeme und die Azofunktion mit.
N2  - Dihydropyridazine 2c was obtained from benzvalene (1) and 3,6- bis(2-pyridyJ)-1,2,4,5-tetrazine. Like the known dihydropyridazines 2a, b. compound 2c was converted into the corresponding azo compound 3 by treatment with cyclopropene. The addition of thiophenol to 3a, c and S yielded the half-cage compounds 4a, c and 6, respectively. In these processes the bicyclobutane system and the azo functionality are involved. Treatment of 7, in which the azo bridge has two bicyclobutane neighbours, with thiophenol and bromotrichloromethane led to the formation of the half-cage compounds 8 and 9, ~spectively. Both the bicyclobuta_ne systems and the azo functionality are transformed in these reactions.
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58440
ER  - 
TY  - JOUR
A1  - Christl, Manfred
A1  - Kraft, A.
T1  - Tricyclo[3.1.1.0\(^{2,6}\)]hexandion (das Valen des o-Benzochinons), Bicyclo[2.1.1]hexan-2,3-dion und Valene eines Chinoxalins, des Phenazins sowie eines Benzophenazins
N2  - No abstract available
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58459
ER  - 
TY  - JOUR
A1  - Christl, Manfred
A1  - Kraft, A.
T1  - Tricyclo[3.1.1.0\(^{2,6}\)]hexandion (the Valen of o-Benzochinons), Bicyclo[2.1.1]hexan-2,3-dion and Valene of a Chinoxalins, of Phenazins and of a Benzophenazine
N2  - No abstract available
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58462
ER  - 
TY  - JOUR
A1  - Schomburg, D.
A1  - Link, M.
A1  - Linoh, H.
A1  - Tacke, Reinhold
T1  - Molekülstruktur der Akarizide Chlortrineophylstannan, Chlortris[(dimethylphenylsilyl)methyl]stannan und Trineophyl(1,2,4-triazol-1-yl)stannan-hemihydrat sowie des 2,5-Dimethyl-2,5-diphenylhexans (Bineophyl)
N2  - Die Kristall- und Molekülstrukturen der Akarizide Chlortrineophylstannan (Ia), Chlortris[ ( dimethylphenylsilyl)methyl]stannan (tb) und Trineophyl(1,2,4-triazol-1- yl)stannan-hemihydrat (2a · 0.5H\(_2\)0) wurden durch Einkristall-Röntgenstrukturanalysen bestimmt. Zu V ergleichszwecken wurde ausserdem die Struktur des 2,5-Dimethyl-2,5-diphenylhexans (Bineophyl, 4) untersucht. Die Knüpfung von drei sehr raumerfüllenden N eophyl-Resten an ein Zinnatom führt zu einer deutlichen Verzerrung der tetraedrischen Geometrie [ta: C-Sn-C 117.2°, C-Sn-Cl99.7°; 2a·0.5H20: C-Sn-C 116.9° (Mittelwert), C-Sn-N 100.2° (Mittelwert)]. Austausch der zentralen Kohlenstoffatome in den Neophyt-Substituenten von la durch Siliciumatome führt zu einer Verringerung des Raumbedarfs und dadurch zu einer erkennbaren Angleichung an die tetraedrische Geometrie [lb: C-Sn-C 113.3° (Mittelwert), C-Sn-Cl 105.3° (Mittelwert)].
N2  - The crystal and molecular structures of the acaricides chlorotrineophylstannane (ta), chlorotris[( dimethylphenylsilyl)methyl]stannane (tb ), and trineophyl(1,2,4-triazol- 1-yl)stannane hemihydrate (2a · 0.5H\(_2\)0), have been determined by single-crystal X-ray diffraction studies. The structure of 2,5-dimethyl-2,5-diphenylhexane (4) was also investigated for comparison. Binding of three very bulky neophyl ligands around tin causes serious distortion of the tetrahedral geometry [la: C-Sn-C 117.2°, C-Sn-Cl 99.7°; la · 0.5H20: C-Sn-C 116.9° (mean), C-Sn-N 100.2° (mean)]. Replacement of the central carbon atoms in the neophyl substituents of ta by silicon atoms Ieads to a decrease in steric strain and hence to a much smaller distortion of the tetrahedral geometry [lb: C-Sn-C 113.3° (mean), C-Sn-Cl105.3° (mean)].
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63840
ER  - 
TY  - JOUR
A1  - Sarge, S.
A1  - Cammenga, H. K.
A1  - Becker, B.
A1  - Rohr-Aehle, R.
A1  - Tacke, Reinhold
T1  - Energetic and kinetic investigation of thermally induced molecular rearrangements of esters of (hydroxymethyl)hydridosilanes by DSC
N2  - No abstract available
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63850
ER  - 
TY  - JOUR
A1  - Lambrecht, G.
A1  - Gmelin, G.
A1  - Rafeiner, K.
A1  - Strohmann, C.
A1  - Tacke, Reinhold
A1  - Mutschler, E.
T1  - o-Methoxy-sila-hexocyclium: a new quaternary M\(_1\)-selective muscarinic antagonist
N2  - No abstract available
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63862
ER  - 
TY  - JOUR
A1  - Lambrecht, G.
A1  - Feifel, R.
A1  - Forth, B.
A1  - Strohmann, C.
A1  - Tacke, Reinhold
A1  - Mutschler, E.
T1  - p-Fluoro-hexahydro-sila-difenidol: the first M\(_{2\beta}\)-selective muscarinic antagonist
N2  - No abstract available
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63872
ER  - 
TY  - JOUR
A1  - Tacke, Reinhold
A1  - Rohr-Aehle, R.
T1  - Ester des (Hydroxymethyl)[(trimethylsilyl)methyl]silans: Synthese und thermisch induzierte Umlagerung
N2  - Die Synthese des (Hydroxymethyl)[(trimethylsilyl)methyl]silans (3) sowie des hiervon abzuleitenden Acetats 4 und Chlorformiats 6 wird beschrieben. 4 und 6 unterliegen einer thermisch induzierten Umwandlung zu den difunktionellen Silanen 5 bzw. 8. Die Umwandlungen 4 -> 5 und 6 -> 8 erfolgen gemäß einer Kinetik 1. Ordnung mit Halbwertszeiten von 10.0 bzw. 3.6 h (135 ° C, in C\(_6\)D\(_6\)).
N2  - The synthesis of (hydroxymethyl)[(trimethylsilyl)methyl]silane (3) and that of the corresponding acetate 4 and chloroformiate 6 are described. 4 and 6 undergo a thermally induced rearrangement to give the difunctional silanes 5 and 8, respectively. The transformations 4 -> 5 and 6 -> 8 follow a first order rate law with half life times of 10.0 and 3.6 h, respectively (135 o C, in C\(_6\)D\(_6\) ).
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63889
ER  - 
TY  - JOUR
A1  - Tacke, Reinhold
A1  - Becker, B.
T1  - Synthese und Eigenschaften von (Hydroxymethyl)dimethylgerman und (Hydroxymethyl)diphenylgerman
N2  - Die (Hydroxymethyl)diorganylgermane (CH\(_3\))\(_2\)Ge(H)CH\(_2\)OH und (C\(_6\)H\(_5\))\(_2\)Ge(H)CH\(_2\)OH wurden - ausgehend von GeCl\(_4\) - durch eine jeweils vierstufige Synthese mit einer Gesamtausbeute von 15 bzw. 32% dargestellt (GeCl\(_4\) ---+ Cl\(_3\)GeCH\(_2\)Cl -> R \(_2\)Ge(Cl)CH \(_2\)Cl ->R \(_2\)Ge(OAc)CH\(_2\)OAc--) R \(_2\)Ge(H)CH\(_2\)OH; R = CH\(_3\) bzw. C\(_6\)H\(_5\) ). Die chemische Reaktivität der Germane (CH\(_3\))\(_2\)Ge(H)CH\(_2\)OH und (C\(_6\)H\(_5\))\(_2\)Ge(H)CH\(_2\)OH wird durch deren Ge-H- und 0-H-Bindung bestimmt.
N2  - Starting from GeCl\(_4\) , the (hydroxymethyl)diorganylgermanes (CH\(_3\)) \(_2\)Ge(H)CH\(_2\)- 0H and (C\(_6\)H\(_5\)) \(_2\)Ge(H)CH\(_2\)OH were prepared by a four-step synthesis in total yields of 15 and 32%, respectively (GeCl\(_4\) ---. Cl\(_3\)GeCH\(_2\)Cl -> R \(_2\)Ge(Cl)CH\(_2\)Cl -> R \(_2\)Ge(OAc)CH\(_2\)OAc--+ R \(_2\)Ge(H)CH\(_2\)OH; R = CH\(_3\) or C\(_6\)H\(_5\) ). The chemical reactivity of (CH\(_3\) ) \(_2\)Ge(H)CH\(_2\)OH and (C\(_6\)H\(_5\)) \(_2\)Ge(H)CH \(_2\)OH was found to be determined by their Ge-Hand 0-H bonds.
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63895
ER  - 
TY  - JOUR
A1  - Syldatk, C.
A1  - Stoffregen, A.
A1  - Wuttke, F.
A1  - Tacke, Reinhold
T1  - Enantioselective reduction of acetyldimethylphenylsilane: a screening with thirty strains of microorganisms
N2  - Thirty strains of microorganisms (bacteria, yeasts, fungi and green algae) were tested as resting free cells for their ability to transform acetyldimethylphenylsilane (1) enantioselectively into (R)-(1-hydroxyethyl) dimethylphenylsilane [(R)-2]. The biotransformations were monitared by GC (packed OV-17 column), and the enantiomeric purities of the products isolated were determined by HPLC (cellulose triacetate column, UV detection). All microorganisms tested were found to reduce 1 enantioselectively to give (R)-2. Under the test conditions used, the yeast Trigonapsis variabilis (DSM 70714) was found to 1 exhibif the highest specific activity (1.5 mg product x g cell wet mass\(^{-1}\) x min\(^{-1}\) ), whereas the highest enantioselectivities were observed for the bacteria Acinetobacter ca lcoaceticus (ATCC 31012) (>95% ee), Brevfbacterium species (ATCC 21860) (90% ee) and Corynebacterium dioxydans (ATCC 21766) (>95% ee), the yeast Candida humico la (OSM 70067) (90% ee), the fungus Cunninghame lla e legans (ATCC 26269) (94% ee), as well as the cyanobacterium Synechococcus leopoliensis (94% ee).· From the green algae tested, Chlamydomonas reinhardii showed the highest.enantioselectivity (85% ee).
KW  - Anorganische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63906
ER  - 
TY  - JOUR
A1  - Eltze, M.
A1  - Gmelin, G.
A1  - Wess, J.
A1  - Strohmann, C.
A1  - Tacke, Reinhold
A1  - Mutschler, E.
A1  - Lambrecht, G.
T1  - Presynaptic muscarinic receptors mediating inhibition of neurogenic contractions in rabbit vas deferens are of the ganglionic M\(_1\)-type
N2  - The present study was designed to further charaeterize the presynaptie musearlnie M\(_1\)-reeeptor responsible for the inhibition of neuragenie eontraetions in the isolated rabbit vas deferens. Eleetrically induced twiteh eontraetions of this preparation were inhibited by the M\(_1\)-agonist, MeN-A-343, and by some of its analogs: 4-ehloro-phenyl derivative> MeN-A-343 > trans-olefinie analog> cis-olefinie analog. The same rank order of potency was observed for these agonists to raise the blood pressure of pithed rats by stimulation of M\(_1\)-receptors in sympathetie ganglia. A highly signifieant eorrelation was found between the antimusearinie potencies of atropine, pirenzepine and a series of 9 antagonists strueturally related to the ganglionie M\(_{1\beta}\)-receptor selective compounds, hexocyclium and hexahydro-difenidol, to antagonize the MeN-A-343-indueed inhibition of twitch eontraetions in rabbit vas deferens or the musearine-indueed depolarization in rat isolated superior eerVieal ganglia. It is suggested that the presynaptie musearlnie receptor that mediates inhibition of neuragenie contraetions in rabbit vas deferens is of the ganglionic M\(_{1\beta}\)-type.
KW  - Anorganische Chemie
KW  - Musearlnie aeetyleholine receptor antagonists
KW  - McN-A-343 analogs
KW  - Musearlnie receptor subtypes
KW  - Vas deferens (rabbit)
KW  - Pithed rat
KW  - Ganglia (rat)
KW  - Musearlnie aeetyleholine receptor agonists
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63912
ER  - 
TY  - JOUR
A1  - Moll, Heidrun
A1  - Scollay, R.
A1  - Mitchell, G. F.
T1  - Resistance to cutaneous leishmaniasis in nude mice injected with L3T4+ T cells but not with Ly-2+ T cells
N2  - No abstract available
KW  - Biologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61269
ER  - 
TY  - JOUR
A1  - Christl, Manfred
A1  - Freund, S.
A1  - Henneberger, H.
A1  - Kraft, A.
A1  - Hauck, J.
A1  - Irngartinger, H.
T1  - Several Polycyclic Valence Isomers of Dimethyl [14]Annulene-1,8-dicarboxylate. Reactivity of a "Nonconjugated" Bis(bicyclo[1.1.0]butane)
N2  - Diels-Alder reaction of dimethyl 1,2,4,5-tetrazine-3,6-dicarboxylate (5) with benzvalene (4), norbornene, and norbornadiene afforded the azo compounds 7 and 8. Theseare derivatives of 2,3-diazabicyclo[2.2.2]oct-2-ene as is azo compound 3, which had been obtained previously from 5 and 2 equiv of benzvalene (4). The photochemical extrusion of nitrogen from 3, 7, and 8 has been studied. Whereas 7 and 8 on direct irradiation in benzene gave rise exclusively to the bicyclo[2.2.0]hexane derivatives 9 and 10, respectively, from 3 in addition to the bicyclo[2.2.0]hexane 11, the diolefin 1l was formed. Diolefin 12 has cisdouble bonds in the nine-membered ring and is fixed in a boat conformation in a manner so that the two bicyclobutane systems approach each other very closely. This geometry suggests the unusual ring opening of the intermediate 1,4-cyclohexanediyl diradical from a boat conformation, which arises by inversion of the primarily generated boat conformation. Sensitized photolysis of 3 as weilasthat of ll produced the saturated isomer 13 of 11 and 12. The proximity of the bicyclobutane systems in 1l causes unprecedented reactions leading to cage compounds. When ll was heated at 90 °C, a rearrangement to the pentacyclic product 10 took place. Utilization of tetradeuteriated substrate ll-d4 supported a pathway with two diradical intermediates. Behaving in a convcntional manncr, bicyclobutane 9 and bis(bicyclobutane) 11 took up 1 and 2 equiv of thiophenol most probably in a radical-chain addition to give the thioethers 28 and 19, respectively. In contrast, bis(bicyclobutane) ll was converted by 1 equiv of thiophenol into cagc compound 30 in a process involving both the strained a systems. Heating at 80 °C subjected 30 to a reversible Copc rearrangement, resulting in a 6:1 mixture of 31 and 30. When it was treated with bromine, 11 was transformed to cage compound 38. This addition is believed to proceed via a cationic intermediate. The structure of cage compound 10 was established by a singlc-crystal X-ray analysis of dialcohol 11 prepared from 20 and methyllithium.
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58413
ER  - 
TY  - JOUR
A1  - Goebel, Werner
A1  - Chakraborty, T.
A1  - Kreft, Jürgen
T1  - Bacterial hemolysins as virulence factors
N2  - No abstract available
KW  - Biologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60553
ER  - 
TY  - JOUR
A1  - Goebel, Werner
A1  - Kathariou, S.
A1  - Kuhn, M.
A1  - Sokolovic, Z.
A1  - Kreft, Jürgen
A1  - Köhler, S.
A1  - Funke, D.
A1  - Chakraborty, T.
A1  - Leimeister-Wächter, M.
T1  - Hemolysin from Listeria-biochemistry, genetics and function in pathogenesis
N2  - No abstract available
KW  - Biologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60563
ER  - 
TY  - JOUR
A1  - Christl, Manfred
A1  - Nusser, R.
A1  - Herzog, C.
T1  - 4-Bromoctavalen und zwei (Brommethylen)homobenzvalene anstelle eines erwarteten Bromoctabisvalens
N2  - No abstract available.
KW  - Organische Chemie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58409
ER  - 
TY  - JOUR
A1  - Lohse, Martin J.
A1  - Klotz, Karl-Norbert
A1  - Salzer, Manfred J.
A1  - Schwabe, Ulrich
T1  - Adenosine regulates the \(Ca^{2+} \) sensitivity of mast cell mediator release : (histamine secretion/inositol phosphates/calcium)
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - Mast cells release histamine and other mediators of allergy in response to stimulation of their IgE receptors. This release is generally thought to be mediated by an elevation of cytosolic \(Ca^{2+}\). Recent evidence suggests that there might be factors that modulate the coupling between \(Ca^{2+}\) levels and mediator release. The present report identifies adenosine as one such modulator. Adenosine and several of its metabolically stable analogues were shown to enhance histamine release from rat peritoneal mast cells in response to stimuli such as concanavalin A. Metabolizing endogenous adenosine with adenosine deaminase dampened the response to stimuli, whereas trapping endogenous adenosine inside mast cells with nucleoside-transport inhibitors markedly enhanced stimulated histamine release. The metabolically stable adenosine analogue 5' -(N-ethylcarboxamido)adenosine (NECA) did not affect the initial steps in the sequence from IgE-receptor activation to mediator release, which are generation of inositol trisphosphate and increase of cytosolic \(Ca^{2+}\). However, NECA did enhance the release induced in ATP-permeabilized cells by exogenous \(Ca^{2+}\), but it had no effect on the release induced by phorbol esters. These data suggest that adenosine sensitizes mediator release by a mechanism regulating stimulus-secretion coupling at a step distal to receptor activation and second-messenger generation.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-127883
VL  - 85
ER  - 
TY  - JOUR
A1  - Pleticha-Geuder, Eva
T1  - Ex Bibliotheca Monasterii Neustatt ad Moenum. Zur Geschichte der Bibliothek von Kloster Neustadt am Main bis zur Säkularisation.
N2  - No abstract available
KW  - Würzburg / Universitätsbibliothek
KW  - Adelsbibliothek
KW  - Ausstellung
KW  - Würzburg <1988>
KW  - Bronnbach / Kloster / Bibliothek
KW  - Kleinheubach
KW  - Neustadt <Main>
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-35469
ER  - 
TY  - CHAP
A1  - Schüssler, Ulrich
A1  - Vejnar, Z.
A1  - Okrusch, Martin
A1  - Rose, D.
A1  - Seidel, E.
T1  - Geochemistry of metabasites and gabbroic rocks from the Tepla-Domazlice zone.
N2  - Various amphibolites, metagabbros and eclogitic relics of the Mariimske Lazne complex, and amphibolites from the Cerna Hora Massif exhibit an uniform geochemical character which compares well with modern mid-ocean ridge basalts. Geochemically these metabasites are similar to the amphibolites of the My to area and to schistose. partly striped amphibolites of the neighbouring Tirschenreuth-Mahring Zone and the Erbendorf-Vohenstrauss Zone (Bavaria). Greenschists and amphibolites from the Domailice metamorphic complex show an alkaline-basaltic tendency conforming to modern within-plate basalts or basalts from anomalous midocean ridge segments. In their chemical character, these metabasites compare well with the flaseramphibolites of the Erbendorf-Vohenstrauss Zone. Fine-grained amphibolites in the Warzenrieth area and gabbroic amphiboltes in the Blatterberg-Hoher Bogen area show normal MORB character. The metamorphosed gabbroic rocks in the southern part of the Neukirchen-Kdyne (meta-) igneous complex are subalkaline-tholeiitic and exhibit a magmatic differentiation trend. They differ from the neighbouring amphibolites by generally lower contents of incompatible elements.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39164
ER  - 
TY  - GEN
T1  - Jahresbericht 1987
T1  - Annual Report 1987
N2  - Die Entwicklung der Universitätsbibliothek Würzburg im Jahr 1987.
N2  - Annual Report of the University Library of Würzburg, 1987.
T3  - Jahresbericht der Universitätsbibliothek Würzburg - 1987 
KW  - Würzburg
KW  - Universitätsbibliothek
KW  - Bericht
KW  - Jahresbericht
KW  - Würzburg
KW  - University Library
KW  - Report
KW  - Annual Report
KW  - Wuerzburg
KW  - Wurzburg
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-42319
ER  - 
TY  - JOUR
A1  - Thiry, Marc
A1  - Scheer, Ulrich
A1  - Goessens, Guy
T1  - Immunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumour cells
N2  - In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus.
KW  - Cytologie
KW  - Nucleolus
KW  - DNA
KW  - mitosis
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40745
ER  - 
TY  - CHAP
A1  - Linsenmair, Karl Eduard
A1  - Mikula, Gerold
T1  - Soziale Einflüsse
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44469
ER  - 
TY  - JOUR
A1  - Hommers, Wilfried
T1  - Die Wirkungen von Entschuldigung und Dritt-Entschädigung auf Strafurteile über zwei Schadensarten
N2  - Kinder aus 3 Altersgruppen und Erwachsene beurteilten die verdiente Strafe aufgrund von Geschichten über zwei aus Wut erfolgte Schädigungen (Sachschaden und Körperschaden). Die Geschichten informierten außerdem darüber, ob sich der Täter entschuldigte oder ob ein Dritter dem Leidtragenden eine materielle Entschädigung gab. Die Dritt-Entschädigungsinformation reduzierte die Strafurteile der Vor- und Grundschüler geringfügig weniger als die Entschuldigungsinformation. Bei Erwachsenen war der Dritt-Entschädigungseffekt jedoch erheblich geringer als der Entschuldigungseffekt. Diese Ergebnisse trafen sowohl auf das mittlere Strafurteil als auch auf den Gebrauch von Strafe zu. Nur die Erwachsenen bestraften den Körperschaden mehr als den' Sachschaden.
N2  - Children of three age groups and adults rated the deserved punishment for vignettes about a property damage and a personal injury, both of which were furiously done. The stories told the subjects also either whether the harmdoer apologized or whether someone else gave a material compensation to the harmed one. For preschoolers and elementary school children, the information about the third-party compensation reduced the punishment a Iittle less than the information about the apology. For adults, however, the effect ofthe thirdparty compensation was much smaller than the effect of the apology. These results were obtained with the mean punishment as well as with the frequencies of the decision to punish at all. Only the adults punished the personal injury more than the property damage.
KW  - Sozialpsychologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43978
ER  - 
TY  - JOUR
A1  - Krüger, Hans-Peter
A1  - Stuckenberg, Annette
A1  - Vollrath, Mark
T1  - Stability and Variability in Interactive Behavior as Measured by Methods of "Speech Chronemics"
N2  - Dyadic interaction is modelled os an adaptive process between personality of the partners involved and the characteristics of the theme. The theme structure and the principles which control the adaptation process are referred to as "syntality". The material of the studies reported are the speech signals of the verbal interaction reduced to an on-off pattern. In a first study individual speech behavior was found to remain stable in dyads even if partners changed. The second study showed the stability of the speech patterns for different interaction themes even if dyads changed. These apparently contradictory results are reconciled by introducing the concept of "adaptive stability". Individual speech behavior does not happen at a stable activity level, but is characterized by a constant relationship (" less" or "more") to the respective activity of the other partner.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40908
ER  - 
TY  - CHAP
A1  - Ellgring, Johann Heinrich
T1  - Emotionen in der Verhaltenstherapie
N2  - Anhand eines geschichtlichen Rückblicks und einer Zusammenfassung jüngerer Emotionstheorien wird die Bedeutung der Emotionen für die Verhaltenstherapie abgeleitet. Die Ursprünge in der verhaltenstherapeutischen Angstbehandlung zeigen, daß für die Verhaltenstherapie seit ihrem Beginn die Veränderung von emotionalen Störungen eine zentrale Aufgabe war. Aus jüngeren Theorien zu diskreten Emotionen und deren Komponenten lassen sich diagnostische und therapeutische Zielrichtungen spezifizieren. Als Komponenten der Emotionen werden die Bewertung der Situation, physiologische Aktivierung, Motivierung, Handlungsvorbereitung, Signal zum Organismus und Signal zur Umgebung erläutert. Von den verschiedenen Funktionen der Emotionen sind die Funktion des Antriebs für unser Verhalten, die Funktion der Koppelung zwischen Reizen und Reaktionen und die verhaltenssteuernde Funktion für die Therapie besonders bedeutsam. Beispiele von verschiedenen Ansätzen auf der somatisch-physiologischen Ebene, der kognitiven Ebene und der Verhaltens-Ebene geben einen Einblick in die Möglichkeiten der Verhaltenstherapiefür die Veränderung negativer emotionaler Zustände. Nach der kognitiven Wende scheint die emotionale Wende in der Verhaltenstherapie angezeigt.
N2  - The relevance of emotions for behavior therapy is derived from a historical view and form a review of current theories on emotions. The origin of behavioral treatment of anxiety reveals thatfrom the beginning, a central task of behavior therapy was the change of emotional disturbances. It is shown that recent theories on discrete emotions and their components allow to derive and specify diagnostic and therapeutic goals. Evaluation of a situation, physiological arousal, motivation, preparation of action, signalling towards the organism and signalling towards the environment are discussed as components of emotions. From the various functions of emotion the function of driving the behavior, the function of coupling between stimuli and responses and the function of regulating the behavior are seen as specially relevant for therapy. The potentials of behavior therapy for changing negative emotional states is shown byexamples of approaches on the somatic-physiologicallevel, the cognitive level, and the behavioral level. After having undergone the cognitive turn it seems appropriate for behavior therapy to also focus on emotions.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41989
ER  - 
TY  - JOUR
A1  - Klawitter, Jörg
T1  - Ökologische Kommunikation: Theorie der Gesellschaft oder Theorie der ökologischen Gefährdung der Gesellschaft?
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44345
ER  - 
TY  - CHAP
A1  - Linsenmair, Karl Eduard
A1  - Tyrell, Hartmann
A1  - Schulze, Hans-Joachim
A1  - Müller, Klaus E.
T1  - Familie und Kleingruppen
N2  - No abstract available
KW  - Psychobiologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44451
ER  - 
TY  - CHAP
A1  - Hommers, Wilfried
A1  - Endres, Johann
T1  - Strafe und Schadensersatz als multivariater Response Komplex
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43895
ER  - 
TY  - JOUR
A1  - Moll, Heidrun
A1  - Mitchell, Graham F.
T1  - Analysis of variables associated with the promotion of resistance, and its abrogation, in T cell-reconstituted nude mice infected with Leishmania major
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30949
ER  - 
TY  - CHAP
A1  - Seidel, E.
A1  - Kreuzer, H.
A1  - Schüssler, Ulrich
A1  - Okrusch, M.
A1  - Lenz, K.-L.
A1  - Raschka, H.
T1  - K-Ar geochronology of the East-Bavarian basement
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-38874
ER  - 
TY  - CHAP
A1  - Schüssler, Ulrich
A1  - Okrusch, M.
A1  - Richter, P.
A1  - Seidel, E.
T1  - Geochemistry of metabasites and element mobility
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-38880
ER  - 
TY  - JOUR
A1  - Schüssler, Ulrich
T1  - Mobilisation einzelner Elemente in Amphiboliten aus der Umgebung der KTB-Lokation, Zone Erbendorf-Vohenstrauss, nördliche Oberpfalz
N2  - No abstract available
KW  - Mineralogie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39248
ER  - 
TY  - JOUR
A1  - Rose, Kathleen M.
A1  - Szopa, Jan
A1  - Han, Fu-Sheng
A1  - Cheng, Yung-Chi
A1  - Richter, Arndt
A1  - Scheer, Ulrich
T1  - Association of DNA topoisomerase I and RNA polymerase I: A possible role for topoisomerase I in ribosomal gene transcription
N2  - RNA polymerase I preparations purified from a rat hepatoma contained DNA topoisomerase activity. The DNA topoisomerase associated with the polymerase had an Mr of 110000, required Mg2+ but not ATP, and was recognized by anti-topoisomerase I antibodies. When added to RNA polymerase I preparations containing topoisomerase activity, anti-topoisomerase I antibodies were able to inhibit the DNA relaxing activity of the preparation as well as RNA synthesis in vitro. RNA polymerase II prepared by analogous procedures did not contain topoisomerase activity and was not recognized by the antibodies. The topoisomerase I: polymerase I complex was reversibly dissociated by column chromatography on Sephacryl S200 in the presence of 0.25 M (NH4hS04. Topoisomerase I was immunolocalized in the transcriptionally active ribosomal gene complex containing RNA polymerase I in situ. These data indicate that topoisomerase I and RNA polymerase I are tightly complexed both in vivo and in vitro, and suggest a role for DNA topoisomerase I in the transcription of ribosomal genes.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-33901
ER  - 
TY  - JOUR
A1  - Reimer, Georg
A1  - Raska, Ivan
A1  - Scheer, Ulrich
A1  - Tan, Eng M.
T1  - Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus
N2  - Certain autoimmune sera contain antibodies against a nucleolar ribonucleoprotein particle associated with 7-2-RNA (R. Reddy et al. (1983) J. Bioi. Chem . 258, 1383; C. Hashimoto and J. A. Steitz (1983) J. Bioi. Chem. 258, 1379). In this study, we showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-I-j3-D-ribofuranosylbenzimidazole (DRB)-segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7- 2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of Mr 40,000 from e5S] methionine-Iabeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-33890
ER  - 
TY  - JOUR
A1  - Benavente, Ricardo
A1  - Schmidt-Zachmann, Marion S.
A1  - Hügle-Dörr, B.
A1  - Reimer, G.
A1  - Rose, K. M.
A1  - Scheer, Ulrich
T1  - Identification and definition of nucleolus-related fibrillar bodies in micronucleated cells
N2  - Small nucleolus-related bodies which occur in the nUcleoplasm of " micronuclei" lacking nucleolar organizers have been studied by immunofluorescence microscopy. These bodies stained specifically with three different antibodies directed against proteins that are normally associated with the dense fibrillar component of functional nucleoli, but not with antibodies specific for certain proteins of the granular component or the fibrillar centers. Our data show that, in the absence of rRNA genes, the various constituent proteins characteristic of the dense fibrillar component spontaneously assemble into spherical entities but that the subsequent fusion of these bodies into larger structures is prevented in these micronuclei. The similarity between these nucleolus-related bodies of micronuclei and the prenucleolar bodies characteristic of early stages of nucleologenesis during mitotic telophase is discussed.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39423
ER  - 
TY  - JOUR
A1  - Thiry, Marc
A1  - Scheer, Ulrich
A1  - Goessens, Guy
T1  - Localization of DNA within Ehrlich tumour cells nucleoli by immunoelectron microscopy
N2  - The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach , involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed . either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In particular, the various nucleolar components are well preserved and identifiable. In the nucleolus, labelling is particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations, which penetrate the nucleolar body and often terminate at the fibrillar centres. In addition, aggregates of gold particles are found in the fibrillar centres, preferentially towards the peripheral regions. By contrast, the dense fibrillar component is completely devoid of labelling. The results seem to indicate that DNA containing the rDNA genes is located in the fibrillar centres, with a preference for the peripheral regions. This finding suggests that transcription of the rDNA genes should occur within the confines of the fibrillar centre, probably close to the boundary region of the surrounding dense fibrillar component. The results are discussed in the light of present knowledge of the functional organization of the nucleolus.
KW  - nucleolus
KW  - DNA
KW  - monoclonal antibody
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39327
ER  - 
TY  - JOUR
A1  - Wirbelauer, J.
A1  - Hof, H.
A1  - Hacker, Jörg
T1  - Die Wirkung von Desacetylcefotaxin, einem Metaboliten von Cefotaxim, in vitro und auf die experimentelle Infektion mit Escherichia coli
T1  - Activity of Desacetylcefotaxime, a Metabolite of Cefotaxime, In Vitro and in a Model of Experimental Infection with Escherichia coli
N2  - Die MHK-Werte von Desacetylcefotaxim gegen verschiedene, z. T. ampicillinresistente Stämme von Escherichia coH, die mit Hilfe einer Agardilutionsmethode erhoben wurden, waren höher als die von Cefotaxim und Ceftriaxon, jedoch niedriger als die von Cefoxitin. In einem Modell der systemischen Infektion der Maus mit einem plasmidtragenden, betalactamaseproduzierenden Stamm von E. coli führte die Therapie mit Desacetylcefotaxim zu einer starken Reduktion der Keime pro Leber. Im Vergleich zur Therapie mit Cefotaxim trat die protektive Wirkung aber verlangsamt ein. Desacetylcefotaxim ist also kein unnützes Abbauprodukt von Cefotaxim.
N2  - The MIC values of desacetylcefotaxime, as determined by an agar dilution method, for several strains of Escherichia coli largely resistant to ampicillin were considerably higher than those of cefotaxime or ceftriaxone respectively. The values were, however, definitely lower than those of cefoxitin. In a mouse model of a systemic infection with a plasmid-bearing, j3-lactamase producing strain of E. coli the therapeutic potency of ampicillin, cefotaxime and desacetylcefotaxime was determined by counting the bacterial numbers per liver at days 1,3 and 7 after subcutaneous infection with about 103 viable bacteria. Desacety1cefotaxime was able to redure thc bacterial load considerably, though with a delay in comparison to cefotaxime.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40348
ER  - 
TY  - JOUR
A1  - Geise, W.
A1  - Linsenmair, Karl Eduard
T1  - Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment. IV. Ecological significance of water economy with comments on thermoregulation and energy allocation
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30570
ER  - 
TY  - JOUR
A1  - Hegmann, Joachim
A1  - Christl, Manfred
A1  - Peters, Karl
A1  - Peters, Eva-Maria
A1  - Schnering, Hans Georg
T1  - Conjugated and Nonconjugated Cyclopentenones by a Reaction Cascade from Methyl 6-0xo-5-phenyl-1,3,4-oxadiazine-2-carboxylate and 1,3-Butadienes
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30207
ER  - 
TY  - JOUR
A1  - Dabauvalle, Marie-Christine
A1  - Schulz, Barbara
A1  - Scheer, Ulrich
A1  - Peters, Reiner
T1  - Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-34288
ER  - 
TY  - JOUR
A1  - Lillien, Laura E.
A1  - Sendtner, Michael
A1  - Rohrer, Hermann
A1  - Hughes, Simon M.
A1  - Raff, Martin C.
T1  - Type-2 Astrocyte Development in Rat Brain Cultures is initiated by a CNTF-like protein produced by type-1 astrocytes
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31708
ER  - 
TY  - JOUR
A1  - Scheer, Ulrich
A1  - Dabauvalle, Marie-Christine
A1  - Merkert, Hilde
A1  - Benavente, Ricardo
T1  - The nuclear envelope and the organization of the pore complexes
N2  - No abstract available
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-34275
ER  - 
TY  - THES
A1  - Schneider-Schaulies, Sibylle
T1  - Molekularbiologische Charakterisierung der Masernvirusreplikation in zentralen Nervensystem von Lewis- und BN-Ratten
N2  - Einleitung: Das Masernvirus (MV) ist ein hochkontagiöser, primatenpathogener Erreger, der für die bekannte Masernerkrankung verantwortlich ist...
N2  - No abstract available.
KW  - Medizin
KW  - Masernvirus
KW  - Immunbiologie
KW  - Masern
KW  - Medicine
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78465
ER  - 
TY  - JOUR
A1  - Laus, R.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Carbohydrate-specific human heterophile antibodies in normal human sera that react with xenogeneic cells
N2  - Human heterophile antibodies (HHA) that are present in normal human sera (NHS)play an important role in hyperacute xenograft rejection. The aim of this study was to analyze the occurrence, mode of action and molecular specificity of HHA in NHS that are directed against xenogeneic Iymphocytes (isolated from mouse, rat, guinea pig, rabbit, cattle and pig) and isolated rat pancreatic islets. All sera contained variable amounts of HHA that killed the target cells via the classical complement pathway. The cytotoxic activity of these HHA was specifically inhibited by certain carbohydrates (a-D-melibiose, ß-Iactose, ß-gentiobiose, ß-cellobiose, D-mannose, N-acetyl-ß-D-mannosamine and a-D-rhamnose) and by rat IgM. By means of affinity chromatography with immobilized inhibitors we obtained an antibody preparation of mainly IgG type from NHS (up to 3.5 mg/IO ml serum) that reacted strongly with rat lymphocytes and isolated rat pancreatic islets. Though thus far residual xenospecific antibody activity has remained in the sera even after multiple affinity chromatography, these data suggest that specific elimination of HHA is feasible and that it may be thus possible to overcome a major obstacle to xenotransplantation.
KW  - Immunobiologie
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78271
ER  - 
TY  - JOUR
A1  - Linsenmair, Karl Eduard
A1  - Schmuck, R.
T1  - Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment. III. Aspects of nitrogen metabolism and osmuregulation in the reed frog, H. viridiflavus taeniatus, with special reference to the role of iridophores
N2  - Reed frogs of the superspecies Hyperolius viridiflavus occur throughout the seasonally very dry and hot African savannas. Despite their small size (300-700 mg), estivating reed frogs do not avoid stressful conditions above ground by burrowing into the soil, but endure the inhospitable climate relatively unprotected, clinging to mostly dry grass sterns. They must have emcient mechanisms to enable them to survive e.g. very high temperatures, low relative hurnidities, and high solar radiation loads. Mechanisms must also have developed to prevent poisoning by the nitrogenous wastes that inevitably result from protein and nucleotide turnover. In contrast to fossorial amphibians, estivating reed frogs do not become torpid. Reduction in metabolism is therefore rather Iimited so that nitrogenous wastes accumulate faster in these frogs than in fossorial amphibians. This severely aggravates the osmotic problems caused by dehydration. During dry periods total plasma osmolarity greatly increases, mainly due to urea accumulation. Of the total urea accumulated over 42 days of experimental water deprivation, 30% was produced during the first 7 days. In the next 7 days rise in plasma urea content was negligible. This strong initial increase of urea is seen as a byproduct of elevated amino acid catabolism following the onset of dry conditions. Tbe rise in total plasma osmolarity due to urea accumulation, however, is not totally disadvantageous, but enables fast rehydration when water is available for very short periods only. Voiding of urine and feces eeases once evaporative water loss exceeds 10% of body weight. Tberefore, during continuous water deprivation, nitrogenous end products are not excreted. After 42 days of water deprivation, bladder fluid was substantially depleted, and urea coneentration in the remaining urine (up to 447 mM) was never greater than in plasma fluid. Feces voided at the end of the dry period after water uptake contained only small amounts of nitrogenous end products. DSF (dry season frogs) seemed not to be uricotelic. Instead, up to 35% of the total nitrogenous wastes produced over 42 days of water deprivation were deposited in an osmotically inert and nontoxic form in iridophore crystals. The increase in skin purine content averaged 150 µg/mg dry weight. If urea had been the only nitrogenous waste product during an estivation period of 42 days, lethal limits of total osmolarity (about 700 mOsm) would have been reached 10-14 days earlier. Thus iridophores are not only involved in colour change and in reducing heat load by radiation remission, but are also important in osmoregulation during dry periods. The seIective advantages of deposition of guanine rather than uric acid are discussed.
KW  - Biologie
KW  - Zoologie
KW  - Frosch
KW  - Hyperolius viridiflavus
KW  - Estivation
KW  - Osmoregulation
KW  - Nitrogen metabolism
KW  - lridophores
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78108
ER  - 
TY  - JOUR
A1  - Schmuck, R.
A1  - Kobelt, F.
A1  - Linsenmair, Karl Eduard
T1  - Adaptations of the reed frog Hyperbolius viridiflavus (Anura, Hyperbolidae) to its arid environment: V. Iridophores and nitrogen metabolism
N2  - Ofall amphibians living in arid habitats, reed frogs (belonging to the super species Hyperolius viridiflavus) are the most peculiar. Froglets are able to tolerate dry periods of up to 35 days or longer immediately after metamorphosis, in climatically exposed positions. They face similar problems to estivating juveniles, i.e. enduranee of long periods of high temperature and low RH with rather limited energy and water reserves. In addition, they must have had to develop meehanisms to prevent poisoning by nitrogenous wastes that rapidly accumulate during dry periods as a metabolie consequenee of maintaining a non-torpid state. During dry periods, plasma osmolarity of H. v. taeniatus froglets strongly increased, mainly through urea accumulation. Urea accumulation was also observed during metamorphic climax. During postmetamorphic growth, chromatophores develop with the density and morphology typical of the adult pigmentary pattern. The dermal iridophore layer, which is still incomplete at this time, is fully developed within 4-8 days after metamorphosis, irrespective of maintenance conditions. These iridophores mainly contain the purines guanine and hypoxanthine. The ability of these purines to reflect light provides an excellent basis for the role of iridophores in temperature regulation. In individuals experiencing dehydration stress, the initial rate of purine synthesis is doubled in eomparison to specimens continuously maintained under wet season conditions. This increase in synthesis rate leads to a rapid increase in the thiekness of the iridophore layer, thereby effectively reducing radiation absorption. Thus, the danger of overheating is diminished during periods of water shortage when evaporative cooling must be avoided. After the development of an iridophore layer of sufficient thickness for effective radiation reflectance, synthesis of iridophore pigments does not cease. Rather, this pathway is further used during the remaining dry season for solving osmotic problems eaused by accumulation of nitrogenous wastes. During prolonged water deprivation, in spite of reduced metabolic rates, purine pigments are produced at the same rate as in wet season conditions. This leads to a higher relative proportion of nitrogen end products being stored in skin pigments under dry season conditions. At the end of an experimental dry season lasting 35 days, up to 38% of the accrued nitrogen is stored in the form of osmotically inactive purines in thc skin. Thus the osmotic problems caused by evaporative water loss and urea production are greatly reduced.
KW  - Biologie
KW  - Zoologie
KW  - Frosch
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78094
ER  - 
TY  - CHAP
A1  - Wehgartner, Irma
T1  - Neue Untersuchungen zur weißgrundigen Lekythenbemalung
N2  - no abstract available
KW  - Lekythenbemalung
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-53807
ER  - 
TY  - JOUR
A1  - Wehgartner, Irma
T1  - Eine neue "pontische" Oinochoe und Überlegungen zur Genese ihrer Form
N2  - Das Martin-von-Wagner-Museum besitzt eine seit langem bekannte Sammlung bedeutender 'pontischer' Vasen, die vor kurzem durch die Erwerbung einer Oinochoe dieser Gattung sinnvoll ergänzt werden konnte (Abb. 30-34. 38. 40-45). Denn obwohl in der 'pontischen' Keramik die Oinochoen nach den Amphoren die häufigste Gefäßform sind, war gerade diese Form in der Würzburger Sammlung bisher nur durch zwei kleine, atypische Exemplare vertreten gewesen. Die neu erworbene Oinochoe kann dagegen nach ihrer Größe, ihren Formmerkmalen und ihrer Bemalung als eine typische Vertreterio dieser Form innerhalb der 'pontischen' Gattung gelten.
KW  - Martin-von-Wagner-Museum / Antikenabteilung
KW  - Oinochoë
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87561
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Leyhausen, G.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - The Electromagnetic Separation Principle: A Basis for Human Pancreatic Islet Transplantation
N2  - No abstract available
KW  - Allergie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-82520
ER  - 
TY  - CHAP
A1  - Lohse, M. J.
A1  - Klotz, K.-N.
A1  - Schwabe, U.
A1  - Christalli, G.
A1  - Vittori, S.
A1  - Grifantini, M.
T1  - Pharmacology and Biochemistry of Adenosine Receptors
N2  - Adenosine modulates a variety of physiological functions via membrane-bound receptors. These receptors couple via G proteins to adenylate cyclase and K+channels. The A1 subtype mediates an inhibition of adenylate cyclase and an opening of K+-channels, and the A2 subtype a Stimulation of adenylate cyclase. Both subtypes have been characterized by radioligand binding. This has facilitated the development of agonists and antagonists with more than 1000-fold A1 selectivity. A1-selective photoaffinity labels have been used for the biochemical characterization of A1 receptors and the study of their coupling to adenylate cyclase. Such selective ligands allow the analysis of the involvement of adenosine receptors in physiological functions. Selective interference with adenosine receptors provides new pharmacological tools and eventually new therapeutic approaches to a number of pathophysiological states.
KW  - Adenosinrezeptor
KW  - Pharmakologie
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86251
ER  - 
TY  - JOUR
A1  - Mäueler, Winfried
A1  - Raulf, Friedrich
A1  - Schartl, Manfred
T1  - Expression of proto-oncogenes in embryonic, adult, and transformed tissue of Xiphophorus (Teleostei: Poeciliidae)
N2  - In Xiphophorus the causative, primary cellular oncogene for melanoma formation has been assigned by classical genetics to a sex-chromosomal locus, designated Tu. Activation of Tu was proposed to be the result of the elimination of Tu-specific regulatory genes which normally suppress the transforming function in the nontumorous state. In order to understand the role which known proto-oncogenes migbt play in this process, we have analysed the expression of src, erb A, erb B, ras, abl, sis and mil related genes from Xiphophorus during embryogenesis, in non-tumorous organs and in melanoma cells. For src, ras, erb B and sis a differential expression during embryogenesis and/or in normal organs was detected, with preferential expression of src in neural tissues, a high abundance of sis transcripts in an embryonal epitheloid cellline and of erbB transcripts in the head nephros. In melanoma cells ras, src and a v-erb B related gene were found to be expressed. The src gene most likely is more involved in secondary processes during tumor progression, while the expression of the v-erb B related gene might be transformation-specific because recently such a sequence was found to map to the close vicinity of the Tu-locus.
KW  - Schwertkärpfling
KW  - Protoonkogen
KW  - Gewebe
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86233
ER  - 
TY  - JOUR
A1  - Maueler, W.
A1  - Barnekow, A.
A1  - Eigenbrodt, E.
A1  - Raulf, F.
A1  - Falk, H. F.
A1  - Telling, A.
A1  - Schartl, Manfred
T1  - Different regulation of oncogene expression in tumor and embryonal cells of Xiphophorus
N2  - Melanoma formation in the poeciliid fish Xiphophorus is mediated primarily by a cellular oncogene, designated Tu. Elimination of Tu-specific genes releases the transforming function of Tu and leads to melanoma formation. Southern blot analyses revealed a tight linkage of a v-erb B related gene to the Tu-locus and Northern blot analyses of RNA of solid melanomas indicated a coordinated deregulation and for mutational activation of several oncogenes. In order to get a better insight into the regulation of oncogene expression in normal and transformed cells of Xiphophorus, we studied the expression of Xsrc, Xras, Xmyc, Xerb A, Xsis, and the v-erb B related gene in a melanoma derived cell line (PSM) and an embryonic cell line (A2) under conditions of low growth factor supply. Both celllines express the Xsrc, Xmyc, and Xras genes, while PSM cells in addition express the v-erb B related gene and A2 cells the Xsis gene. In PSM cells serum deprivation leads to an accumulation of most of the oncogene mRNAs analysed. This is most apparent for a 5.0 kb transcript of the v-erb B related gene, probably due to an increase in transcript stability. The levels of these mRNAs returned to normal within 2h after stimulation with 10% fetal calf serum. At the protein level we observed an initial decrease followed by an increase of the n-p60c-src kinase (the protein product of tbe Xsrc gene) activity in cells deprived of serum. Serum stimulation restored a normal pp60"-src kinase activity. In contrast serum deprivation of A2 cells reduced the transcript amounts of each of the oncogenes analysed. The same holds true for one beta-tubulin transcript, while the level of a second beta-tubulin transcript was unaffected. Serum stimulation led to a reactivation of Xras and Xsrc after a delay of approximately 48b. The pp60(c-src) kinase activity was found to be 6-10 times lower as compared to the PSM cells and did not differ between serum deprived and serum stimulated cells. Enzyme activities and isoenzyme patterns of several glycolytic enzymes were found to be not affected by serum deprivation and stimulation in both celllines.
KW  - Schwertkärpfling
KW  - Tumorzelle
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86240
ER  - 
TY  - JOUR
A1  - Benavente, Ricardo
A1  - Reimer, Georg
A1  - Rose, Kathleen M.
A1  - Hügle-Dörr, Barbara
A1  - Scheer, Ulrich
T1  - Nucleolar changes after microinjection of antibodies to RNA polymerase I into the nucleus of mammalian cells
N2  - After microinjection of antibodies against RNA polymerase I into the nuclei of cultured rat kangaroo (PtKz) and rat (RVF-SMC) cells alterations in nucleolar structure and composition were observed. These were detected by electron microscopy and double-label immunofluorescence microscopy using antibodies to proteins representative of the three major components of the nucleolus. The microinjected antibodies produced a progressive loss of the material of the dense fibrillar component (DFC) from the nucleoli which, at 4 h after injection, were transformed into bodies with purely granular component (GC) structure with attached fibrillar centers (FCs). Concomitantly, numerous extranucleolar aggregates appeared in the nucleoplasm which morphologically resembled fragments of the DFC and contained a protein (fibrillarin) diagnostic for this nucleolar structure. These observations indicate that the topological distribution of the material constituting the DFC can be experimentally influenced in interphase cells, apparently by modulating the transcriptional activity of the rRNA genes. These effects are different from nucleolar lesions induced by inhibitory drugs such as actinomycin D-dependent "nucleolar segregation". The structural alterations induced by antibodies to RNA polymerase I resemble, however, the initial events of nucleolar disintegration during mitotic prophase.
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40666
ER  -