Sterilization Methods and Their Influence on Physicochemical Properties and Bioprinting of Alginate as a Bioink Component
Please always quote using this URN: urn:nbn:de:bvb:20-opus-229460
- Bioprinting has emerged as a valuable threedimensional (3D) biomanufacturing method to fabricate complex hierarchical cell-containing constructs. Spanning from basic research to clinical translation, sterile starting materials are crucial. In this study, we present pharmacopeia compendial sterilization methods for the commonly used bioink component alginate. Autoclaving (sterilization in saturated steam) and sterile filtration followed by lyophilization as well as the pharmacopeia non-compendial method, ultraviolet (UV)-irradiation forBioprinting has emerged as a valuable threedimensional (3D) biomanufacturing method to fabricate complex hierarchical cell-containing constructs. Spanning from basic research to clinical translation, sterile starting materials are crucial. In this study, we present pharmacopeia compendial sterilization methods for the commonly used bioink component alginate. Autoclaving (sterilization in saturated steam) and sterile filtration followed by lyophilization as well as the pharmacopeia non-compendial method, ultraviolet (UV)-irradiation for disinfection, were assessed. The impact of the sterilization methods and their effects on physicochemical and rheological properties, bioprinting outcome, and sterilization efficiency of alginate were detailed. Only sterile filtration followed by lyophilization as the sterilization method retained alginate's physicochemical properties and bioprinting behavior while resulting in a sterile outcome. This set of methods provides a blueprint for the analysis of sterilization effects on the rheological and physicochemical pattern of bioink components and is easily adjustable for other polymers used in the field of biofabrication in the future.…
Author: | Thomas Lorson, Matthias Ruopp, Ali Nadernezhad, Julia Eiber, Ulrich Vogel, Tomasz Jungst, Tessa Lühmann |
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URN: | urn:nbn:de:bvb:20-opus-229460 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Hygiene und Mikrobiologie |
Fakultät für Chemie und Pharmazie / Institut für Pharmazie und Lebensmittelchemie | |
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde | |
Language: | English |
Parent Title (English): | ACS Omega |
Year of Completion: | 2020 |
Volume: | 5 |
Issue: | 12 |
Pagenumber: | 6481-6486 |
Source: | ACS Omega 2020, 5, 6481−6486. DOI:10.1021/acsomega.9b04096 |
DOI: | https://doi.org/10.1021/acsomega.9b04096 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | hydrogels |
Release Date: | 2021/04/22 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2020 |
Note: | Lizenz: https://pubs.acs.org/page/policy/authorchoice_termsofuse.html |