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Chagas disease: detection of Trypanosoma cruzi by a new, high-specific real time PCR
Please always quote using this URN: urn:nbn:de:bvb:20-opus-205746
- Background: Chagas disease (CD) is a major burden in Latin America, expanding also to non-endemic countries. A gold standard to detect the CD causing pathogen Trypanosoma cruzi is currently not available. Existing real time polymerase chain reactions (RT-PCRs) lack sensitivity and/or specificity. We present a new, highly specific RT-PCR for the diagnosis and monitoring of CD. Material and Methods: We analyzed 352 serum samples from Indigenous people living in high endemic CD areas of Colombia using three leading RT-PCRs (k-DNA-, TCZ-, 18SBackground: Chagas disease (CD) is a major burden in Latin America, expanding also to non-endemic countries. A gold standard to detect the CD causing pathogen Trypanosoma cruzi is currently not available. Existing real time polymerase chain reactions (RT-PCRs) lack sensitivity and/or specificity. We present a new, highly specific RT-PCR for the diagnosis and monitoring of CD. Material and Methods: We analyzed 352 serum samples from Indigenous people living in high endemic CD areas of Colombia using three leading RT-PCRs (k-DNA-, TCZ-, 18S rRNA-PCR), the newly developed one (NDO-PCR), a Rapid Test/enzyme-linked immuno sorbent assay (ELISA), and immunofluorescence. Eighty-seven PCR-products were verified by sequence analysis after plasmid vector preparation. Results: The NDO-PCR showed the highest sensitivity (92.3%), specificity (100%), and accuracy (94.3%) for T. cruzi detection in the 87 sequenced samples. Sensitivities and specificities of the kDNA-PCR were 89.2%/22.7%, 20.5%/100% for TCZ-PCR, and 1.5%/100% for the 18S rRNA-PCR. The kDNA-PCR revealed a 77.3% false positive rate, mostly due to cross-reactions with T. rangeli (NDO-PCR 0%). TCZ- and 18S rRNA-PCR showed a false negative rate of 79.5% and 98.5% (NDO-PCR 7.7%), respectively. Conclusions: The NDO-PCR demonstrated the highest specificity, sensitivity, and accuracy compared to leading PCRs. Together with serologic tests, it can be considered as a reliable tool for CD detection and can improve CD management significantly.…
Author: | Simone Kann, Meik Kunz, Jessica Hansen, Jürgen Sievertsen, Jose J. Crespo, Aristides Loperena, Sandra Arriens, Thomas Dandekar |
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URN: | urn:nbn:de:bvb:20-opus-205746 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Journal of Clinical Medicine |
ISSN: | 2077-0383 |
Year of Completion: | 2020 |
Volume: | 9 |
Issue: | 5 |
Article Number: | 1517 |
Source: | Journal of Clinical Medicine (2020) 9:5, 1517. https://doi.org/10.3390/jcm9051517 |
DOI: | https://doi.org/10.3390/jcm9051517 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | Chagas diagnosis; Chagas disease; Chagas monitoring; Chagas real time PCR; Trypanosoma cruzi |
Release Date: | 2022/06/01 |
Date of first Publication: | 2020/05/18 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |