Characterization of a New Mouse Model for Peripheral T Cell Lymphoma in Humans
Please always quote using this URN: urn:nbn:de:bvb:20-opus-137946
- Peripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8\(^+\) CD4\(^−\) αβ T cell receptor Vβ2\(^+\) CD28\(^+\) T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells inducedPeripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8\(^+\) CD4\(^−\) αβ T cell receptor Vβ2\(^+\) CD28\(^+\) T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells induced terminal malignancy upon adoptive transfer into syngeneic BALB/c mice. Despite intracellular expression of the cytotoxic T cell differentiation marker granzyme B, T8-28 cells appeared to be defective with respect to cytotoxic activity as read-out in vitro. Among the protocols tested, only addition of interleukin 2 in vitro could partially compensate for the in vivo micro-milieu in promoting growth of the T8-28 lymphoma cells.…
Author: | Niklas Beyersdorf, Sandra Werner, Nelli Wolf, Thomas Herrmann, Thomas Kerkau |
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URN: | urn:nbn:de:bvb:20-opus-137946 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
Language: | English |
Parent Title (English): | PLoS One |
Year of Completion: | 2011 |
Volume: | 6 |
Issue: | 12 |
Pagenumber: | e28546 |
Source: | PLoS ONE 6(12): e28546. doi:10.1371/journal.pone.0028546 |
DOI: | https://doi.org/10.1371/journal.pone.0028546 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | T cells; cell staining; cytotoxic T cells; fluorescence-activated cell sorting; interleukins; lymph nodes; lymphomas; mouse models |
Release Date: | 2016/08/29 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2011 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |