Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum

Please always quote using this URN: urn:nbn:de:bvb:20-opus-189279
  • Polygonum cuspidatum (Japanese knotweed, also known as Huzhang in Chinese), a plant that produces bioactive components such as stilbenes and quinones, has long been recognized as important in traditional Chinese herbal medicine. To better understand the biological features of this plant and to gain genetic insight into the biosynthesis of its natural products, we assembled a draft genome of P. cuspidatum using Illumina sequencing technology. The draft genome is ca. 2.56 Gb long, with 71.54% of the genome annotated as transposable elements.Polygonum cuspidatum (Japanese knotweed, also known as Huzhang in Chinese), a plant that produces bioactive components such as stilbenes and quinones, has long been recognized as important in traditional Chinese herbal medicine. To better understand the biological features of this plant and to gain genetic insight into the biosynthesis of its natural products, we assembled a draft genome of P. cuspidatum using Illumina sequencing technology. The draft genome is ca. 2.56 Gb long, with 71.54% of the genome annotated as transposable elements. Integrated gene prediction suggested that the P. cuspidatum genome encodes 55,075 functional genes, including 6,776 gene families that are conserved in the five eudicot species examined and 2,386 that are unique to P. cuspidatum. Among the functional genes identified, 4,753 are predicted to encode transcription factors. We traced the gene duplication history of P. cuspidatum and determined that it has undergone two whole-genome duplication events about 65 and 6.6 million years ago. Roots are considered the primary medicinal tissue, and transcriptome analysis identified 2,173 genes that were expressed at higher levels in roots compared to aboveground tissues. Detailed phylogenetic analysis demonstrated expansion of the gene family encoding stilbene synthase and chalcone synthase enzymes in the phenylpropanoid metabolic pathway, which is associated with the biosynthesis of resveratrol, a pharmacologically important stilbene. Analysis of the draft genome identified 7 abscisic acid and water deficit stress-induced protein-coding genes and 14 cysteine-rich transmembrane module genes predicted to be involved in stress responses. The draft de novo genome assembly produced in this study represents a valuable resource for the molecular characterization of medicinal compounds in P. cuspidatum, the improvement of this important medicinal plant, and the exploration of its abiotic stress resistance.show moreshow less

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Metadaten
Author: Yonghong Zhang, Lanlan Zheng, Yan Zheng, Chao Zhou, Ping Huang, Xiao Xiao, Yongheng Zhao, Xincai Hao, Zhubing Hu, Qinhua Chen, Hongliang Li, Xuanbin Wang, Kenji Fukushima, Guodong Wang, Chen Li
URN:urn:nbn:de:bvb:20-opus-189279
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Parent Title (English):Frontiers in Plant Science
ISSN:1664-462X
Year of Completion:2019
Volume:10
Pagenumber:1274
Source:Frontiers in Plant Science, 2019, 10:1274. doi: 10.3389/fpls.2019.01274
DOI:https://doi.org/10.3389/fpls.2019.01274
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen
5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Tag:Polygonum cuspidatum; genome assembly; medicinal plant; resveratrol biosynthesis; stress tolerance; whole-genome duplication
Release Date:2019/10/22
Date of first Publication:2019/10/18
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International