Refine
Has Fulltext
- yes (8)
Is part of the Bibliography
- yes (8)
Document Type
- Journal article (7)
- Doctoral Thesis (1)
Language
- English (8)
Keywords
- Expansion microscopy (2)
- imaging (2)
- Hochauflösende Mikroskopie (1)
- Microscopy (1)
- Mikroskopie (1)
- Super-resolution microscopy (1)
- actin (1)
- acute myeloid leukaemia (1)
- bacterial infection (1)
- cancer imaging (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (8)
- Medizinische Klinik und Poliklinik II (2)
- Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde (1)
- Graduate School of Life Sciences (1)
- Institut für Hygiene und Mikrobiologie (1)
- Institut für Organische Chemie (1)
- Julius-von-Sachs-Institut für Biowissenschaften (1)
- Pathologisches Institut (1)
- Physikalisches Institut (1)
- Rudolf-Virchow-Zentrum (1)
Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2,3,4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.