Refine
Has Fulltext
- yes (12)
Is part of the Bibliography
- yes (12)
Year of publication
Document Type
- Journal article (10)
- Doctoral Thesis (2)
Language
- English (12) (remove)
Keywords
- receptor (12) (remove)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (3)
- Institut für Klinische Neurobiologie (2)
- Julius-von-Sachs-Institut für Biowissenschaften (2)
- Medizinische Klinik und Poliklinik I (2)
- Graduate School of Life Sciences (1)
- Institut für Molekulare Infektionsbiologie (1)
- Lehrstuhl für Biochemie (1)
- Neurologische Klinik und Poliklinik (1)
- Rudolf-Virchow-Zentrum (1)
EU-Project number / Contract (GA) number
- 201099 (1)
Background:
Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo.
Methods:
In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection.
Results:
We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection.
Conclusions:
Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.