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- Cell signalling (1)
- ChIP-seq (1)
- Dendritic cells (1)
- Disease genetics (1)
- Medicine (1)
- Nucleus (1)
- T cells (1)
- TCF1 (1)
- TCF12 (1)
- WASp (1)
- Wiskott-Aldrich syndrome (1)
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- 249177 (1)
Background
The Wiskott–Aldrich syndrome protein (WASp) family of actin-nucleating factors are present in the cytoplasm and in the nucleus. The role of nuclear WASp for T cell development remains incompletely defined.
Methods
We performed WASp chromatin immunoprecipitation and deep sequencing (ChIP-seq) in thymocytes and spleen CD4\(^+\) T cells.
Results
WASp was enriched at genic and intergenic regions and associated with the transcription start sites of protein-coding genes. Thymocytes and spleen CD4\(^+\) T cells showed 15 common WASp-interacting genes, including the gene encoding T cell factor (TCF)12. WASp KO thymocytes had reduced nuclear TCF12 whereas thymocytes expressing constitutively active WASp\(^{L272P}\) and WASp\(^{I296T}\) had increased nuclear TCF12, suggesting that regulated WASp activity controlled nuclear TCF12. We identify a putative DNA element enriched in WASp ChIP-seq samples identical to a TCF1-binding site and we show that WASp directly interacted with TCF1 in the nucleus.
Conclusions
These data place nuclear WASp in proximity with TCF1 and TCF12, essential factors for T cell development.