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- Neisseria meningitidis (3)
- sphingolipids (2)
- Infektion (1)
- Neisseria gonorrhoeae (1)
- Pathogenität (1)
- Sphingolipide (1)
- antimicrobial (1)
- antimicrobials (1)
- biological techniques (1)
- gangliosides and lipid rafts (1)
- host-pathogen interaction (1)
- host–pathogen interaction (1)
- imaging (1)
- microbiology (1)
- microbiology techniques (1)
- microscopy (1)
- single-molecule tracking (1)
- super-resolution microscopy (1)
Sphingolipids, including ceramides, are a diverse group of structurally related lipids composed of a sphingoid base backbone coupled to a fatty acid side chain and modified terminal hydroxyl group. Recently, it has been shown that sphingolipids show antimicrobial activity against a broad range of pathogenic microorganisms. The antimicrobial mechanism, however, remains so far elusive. Here, we introduce 'click-AT-CLEM', a labeling technique for correlated light and electron microscopy (CLEM) based on the super-resolution array tomography (srAT) approach and bio-orthogonal click chemistry for imaging of azido-tagged sphingolipids to directly visualize their interaction with the model Gram-negative bacterium Neisseria meningitidis at subcellular level. We observed ultrastructural damage of bacteria and disruption of the bacterial outer membrane induced by two azido-modified sphingolipids by scanning electron microscopy and transmission electron microscopy. Click-AT-CLEM imaging and mass spectrometry clearly revealed efficient incorporation of azido-tagged sphingolipids into the outer membrane of Gram-negative bacteria as underlying cause of their antimicrobial activity.