Refine
Has Fulltext
- yes (15)
Is part of the Bibliography
- yes (15)
Document Type
- Journal article (15) (remove)
Language
- English (15)
Keywords
- megakaryocytes (4)
- hematology (2)
- platelet (2)
- platelets (2)
- thrombopoiesis (2)
- B cell (1)
- B cell maturation (1)
- B cell receptor (1)
- CD147 (1)
- COVID-19 (1)
- CXCL12-abundant reticular (CAR)-cells (1)
- Canis lupus familiaris (1)
- Cyclophilin A (1)
- Cytoskeleton (1)
- EMMPRIN (1)
- IgD (1)
- IgM (1)
- Tet‐inducible system (1)
- ZIP (1)
- acetylation (1)
- acute Respiratory Distress Syndrome (1)
- algorithm (1)
- bioinformatics (1)
- bleding disorders other than hemophilia (1)
- blood platelets (1)
- bone marrow (1)
- complex (1)
- disease (1)
- dog (1)
- early diagnosis (1)
- endothelial cell (1)
- genetic modification (1)
- glycoprotein Ib (1)
- glycoprotein VI (1)
- guidelines (1)
- hemostasis and thrombosis (1)
- homeostasisIon channels (1)
- iPS cells (1)
- immunoglobulin repertoire (1)
- impedance aggregometry; WHOLE-BLOOD THROMBOELASTOMETRY; DEFINITION; DISEASE (1)
- influenza (1)
- integrin α2 (1)
- interaction (1)
- intracranial bleeding (1)
- ischemic stroke (1)
- laboratory animals (1)
- lncRNAs (1)
- lung cancer (1)
- lung injury (1)
- miRNAs (1)
- microvasculature (1)
- non-invasive biomarkers (1)
- pigmentation (1)
- platelet disorders (1)
- pneumonia (1)
- point of care testing (1)
- recombinant tissue-type plasminogen activator (1)
- regulatory circuit downstream (1)
- retroviral vectors (1)
- signaling (1)
- therapy (1)
- thrombin (1)
- thrombocyte (1)
- thrombocytopenia (1)
- thromboelastometry (1)
- thrombosis (1)
- transient middle cerebral artery occlusion (1)
- zinc (1)
Institute
- Institut für Experimentelle Biomedizin (14)
- Rudolf-Virchow-Zentrum (5)
- Kinderklinik und Poliklinik (2)
- Medizinische Klinik und Poliklinik II (2)
- Theodor-Boveri-Institut für Biowissenschaften (2)
- Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde (1)
- Institut für Anatomie und Zellbiologie (1)
- Institut für Humangenetik (1)
- Institut für diagnostische und interventionelle Neuroradiologie (ehem. Abteilung für Neuroradiologie) (1)
- Klinik und Poliklinik für Anästhesiologie (ab 2004) (1)
Sonstige beteiligte Institutionen
EU-Project number / Contract (GA) number
- 602300 (1)
Zinc (Zn2+) is considered as important mediator of immune cell function, thrombosis and haemostasis. However, our understanding of the transport mechanisms that regulate Zn2+ homeostasis in platelets is limited. Zn2+ transporters, ZIPs and ZnTs, are widely expressed in eukaryotic cells. Using mice globally lacking ZIP1 and ZIP3 (ZIP1/3 DKO), our aim was to explore the potential role of these Zn2+ transporters in maintaining platelet Zn2+ homeostasis and in the regulation of platelet function. While ICP-MS measurements indicated unaltered overall Zn2+ concentrations in platelets of ZIP1/3 DKO mice, we observed a significantly increased content of FluoZin3-stainable free Zn2+, which, however, appears to be released less efficiently upon thrombin-stimulated platelet activation. On the functional level, ZIP1/3 DKO platelets exhibited a hyperactive response towards threshold concentrations of G protein-coupled receptor (GPCR) agonists, while immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptor agonist signalling was unaffected. This resulted in enhanced platelet aggregation towards thrombin, bigger thrombus volume under flow ex vivo and faster in vivo thrombus formation in ZIP1/3 DKO mice. Molecularly, augmented GPCR responses were accompanied by enhanced Ca2+ and PKC, CamKII and ERK1/2 signalling. The current study thereby identifies ZIP1 and ZIP3 as important regulators for the maintenance of platelet Zn2+ homeostasis and function.