Refine
Year of publication
- 2014 (30) (remove)
Document Type
- Doctoral Thesis (30) (remove)
Language
- English (30) (remove)
Keywords
- Bestäuber (2)
- Biodiversität (2)
- Bioinformatik (2)
- Epigenetik (2)
- Hearing loss (2)
- Hörverlust (2)
- Kilimandscharo (2)
- Kilimanjaro (2)
- MAP-Kinase (2)
- Maus (2)
- Molekularbiologie (2)
- Regulation (2)
- Taufliege (2)
- diversity (2)
- phosphorylation (2)
- 3D (1)
- 3D microscopy (1)
- Aberration (1)
- Ackerrandstreifen (1)
- Ameisen (1)
- Apoptosis (1)
- Arten-Energy-Theory (1)
- Bestäubungsökologie (1)
- Bienen <Überfamilie> (1)
- Bildauflösung (1)
- Bioinformatics (1)
- Biologische Uhr (1)
- Blattschneiderameisen (1)
- Botanischer Garten (1)
- Butterfly (1)
- CK2 (1)
- Chlamydia (1)
- Chlamydia trachomatis (1)
- Chlamydia-trachomatis-Infektion (1)
- Chromatin (1)
- Chromosomal Passenger Complex (1)
- Circadian Rhythms (1)
- Cord blood-derived hematopoietic stem and progenitor cells (1)
- Cytoskeleton Chromosomal Passenger Complex Interaction GAR Domain (1)
- DNS-Schädigung (1)
- DOT1 methyltransferase (1)
- Demethylierung (1)
- Deregulierung (1)
- Deutschland (1)
- Dimension 3 (1)
- Diversity (1)
- Drosophila (1)
- Drosophila melanogaster (1)
- Dysplasie (1)
- ERK (1)
- Einzelmolekülmikroskopie (1)
- Embryonale Stammzelle (1)
- Epigenetic (1)
- Evolution (1)
- Fluoreszenzmikroskopie (1)
- Foldamere (1)
- Foldamers (1)
- Forest management (1)
- GAS2L3 (1)
- Gen notch (1)
- Gen-Knockout (1)
- Gene regulation (1)
- Genexpression (1)
- Genregulation (1)
- HPA Axis (1)
- Hematopoietic stem cell ex-vivo expansion (1)
- Hey Proteine (1)
- Hey proteins (1)
- Histon-Methyltransferase (1)
- Histone (1)
- Hochauflösendes Verfahren (1)
- Hydra <Polyp> (1)
- Hypophysen-Zwischenhirn-System (1)
- Hypothalamisch-hypophysäre Achse (1)
- Hämatopoese (1)
- Höhengradient (1)
- Il 4 (1)
- Innere Uhr (1)
- KSR1 (1)
- Knockout <Molekulargenetik> (1)
- Knockout mouse (1)
- Knorpelzelle (1)
- Konstruktive Didaktik (1)
- Käfer (1)
- LCK (1)
- Landnutzungsgradient (1)
- Lernort (1)
- MAP Kinase Signaling (1)
- MAPK (1)
- Makrophage (1)
- Malaria (1)
- Massentrachten (1)
- Mbm (1)
- Meiose (1)
- Mesenchymzelle (1)
- Metabolic Modelling (1)
- Metabolischen Modellierung (1)
- Methylene blue (1)
- Mikroskopie (1)
- Modellierung (1)
- Modifizierung (1)
- Molekulargenetik (1)
- Mutagenese (1)
- Myc (1)
- N-Myc (1)
- Neisseria gonorrhoeae (1)
- Nestbau (1)
- Neuroblast (1)
- Neuroblastom (1)
- Neuropeptide (1)
- Notch Signalweg (1)
- Notch signalling (1)
- Out-of-school learning settings (1)
- PALM (1)
- PEG chemical modification (1)
- PPD (1)
- PRC2 (1)
- Phosphoproteine (1)
- Pollination (1)
- Protein p53 (1)
- RNS-Interferenz (1)
- Raps (1)
- Renin Angiotensin System (1)
- Renin-Angiotensin-Aldosteron-System (1)
- Renin-Angiotensin-System (1)
- SPR-Spektroskopie (1)
- SPRED2 (1)
- Saproxylic beetles (1)
- Saproxylophage (1)
- Schwebfliegen (1)
- Signaltransduktion (1)
- Sonnenblumen (1)
- Spectral Data Analysis (1)
- Spred Protein (1)
- Spred-Proteine (1)
- Stationenarbeit (1)
- Stoffwechsel (1)
- Synaptinemal-Komplex (1)
- TLR4 (1)
- Tanzania (1)
- Termiten (1)
- Thrombozyt (1)
- Toll-like-Rezeptoren (1)
- Transkriptionsfaktor (1)
- Transposon (1)
- Trypanosoma brucei (1)
- Virulenzfaktor (1)
- Vorläuferzellen (1)
- Wald (1)
- Werk (1)
- Zellskelett (1)
- Zellteilung (1)
- Zellzyklus (1)
- Zutokin (1)
- aberration (1)
- ants (1)
- apoptosis (1)
- bioinformatic (1)
- botanical gardens (1)
- cancer (1)
- cell death (1)
- cell growth (1)
- chemische Modifizierung (1)
- chondrocytes (1)
- concept maps (1)
- conceptual change (1)
- cul3 ring ligase (1)
- cytoskeleton (1)
- dSTORM (1)
- developing country (1)
- diagnosis (1)
- ecology (1)
- ecosystem service (1)
- elevational gradient (1)
- environmental cues (1)
- essential genes (1)
- fluorescence (1)
- homology modeling (1)
- human impact (1)
- leaf-cutting ant (1)
- learning at workstations (1)
- localization microscopy (1)
- macrophage (1)
- mass-flowering crops (1)
- menschlicher Einfluss (1)
- mesenchymal stem cells (1)
- metabolism (1)
- miRNA (1)
- miRNS (1)
- naturnahe Habitate (1)
- nest building (1)
- pollinators (1)
- product specificity (1)
- proliferation (1)
- replicative stress (1)
- ribosome biogenesis (1)
- saproxylic Coleoptera (1)
- scientific computing (1)
- secreted effector protein (1)
- self-organization (1)
- semi-natural habitats (1)
- signaling (1)
- single molecule microscopy (1)
- socioeconomic (1)
- species-energy-theory (1)
- sunflowers (1)
- super-resolution (1)
- super-resolution microscopy (1)
- symbiotic fungus (1)
- synthetic lethality (1)
- synthetische Letalität (1)
- temperate forests (1)
- temporal spillover (1)
- termites (1)
- therapy (1)
- transposon mutagenesis (1)
- tropical ecology (1)
- tropische Ökologie (1)
- two-color microscopy (1)
- tyrosine phosphorylation (1)
- virulence factors (1)
- zeitlicher Spillover (1)
- Ökosystem (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (30) (remove)
Sonstige beteiligte Institutionen
This thesis reviews the fundamentals of three-dimensional super-resolution localization imaging. In order to infer the axial coordinate of the emission of single fluorophores, the point spread function is engineered following a technique usually referred to as astigmatic imaging by the introduction of a cylindrical lens to the detection path of a microscope.
After giving a short introduction to optics and localization microscopy, I outline sources of aberrations as frequently encountered in 3D-localization microscopy and will discuss their respective impact on the precision and accuracy of the localization process. With the knowledge from these considerations, experiments were designed and conducted to verify the validity of the conclusions and to demonstrate the abilities of the proposed microscope to resolve biological structures in the three spatial dimensions. Additionally, it is demonstrated that measurements of huge volumes with virtually no aberrations is in principle feasible.
During the course of this thesis, a new method was introduced for inferring axial coordinates. This interpolation method based on cubic B-splines shows superior performance in the calibration of a microscope and the evaluation of subsequent measurement and will therefore be used and explained in this work.
Finally, this work is also meant to give future students some guidance for entering the field of 3D localization microscopy and therefore, detailed protocols are provided covering the specific aspects of two color 3D localization imaging.