Refine
Has Fulltext
- yes (82)
Is part of the Bibliography
- yes (82)
Year of publication
- 2017 (82) (remove)
Document Type
- Journal article (65)
- Doctoral Thesis (17)
Keywords
- biology (8)
- Apis mellifera (4)
- Trypanosoma (4)
- ants (4)
- social systems (4)
- Drosophila (3)
- Drosophila melanogaster (3)
- animal sociality (3)
- fungi (3)
- symbiosis (3)
- Biology (2)
- Chlamydia trachomatis (2)
- DNA damage (2)
- DNA methylation (2)
- Latrophilin (2)
- Neisseria (2)
- Trypanosoma brucei (2)
- carbon dioxide (2)
- cell differentiation (2)
- ceramide (2)
- chemotherapy (2)
- comparative genomics (2)
- cytokines (2)
- fibroblasts (2)
- foraging (2)
- genetic variation (2)
- humidity (2)
- learning (2)
- lung cancer (2)
- memory (2)
- metabolism (2)
- methylation (2)
- mushroom bodies (2)
- nesting habits (2)
- pollen (2)
- toxins (2)
- 3D Ko-kulture (1)
- 3D cell culture (1)
- 3D-Zellkulturen (Krebstherapie) (1)
- 5-fluorouracil (1)
- Acyrthosiphon pisum (1)
- Adhesion-GPCR (1)
- Agrobacterium vitis (1)
- Angewandte Mikrobiologie (1)
- Angiotensin II (1)
- Angiotensin II Typ 1a-Rezeptor (1)
- Antimikrobieller Wirkstoff (1)
- Apoptosis (1)
- Aspergillus fumigatus (1)
- B cell receptors (1)
- B cells (1)
- BMP (1)
- BRAF inhibition (1)
- Bauchspeicheldrüsenkrebs (1)
- Bruchpilot (1)
- Burkina Faso (1)
- CHO cell culture (1)
- CHO-Zelle (1)
- Caenorhabditis elegans (1)
- Cell surface proteomics (1)
- Chlamydia (1)
- Circadian rhythms and sleep (1)
- CoA (1)
- Complexin (1)
- Computer software (1)
- DNA Barcoding (1)
- DNS-Schädigung (1)
- DREAM complex (1)
- Detektion (1)
- Diagnostik (1)
- Drugtargets (1)
- Dunce isoforms (1)
- EZH2 (1)
- Enzyme Regulation (1)
- Enzyme kinetics (1)
- Enzyme metabolism (1)
- Enzyme regulation (1)
- Enzymes (1)
- Epicardium-derived cells (1)
- Epigenetics (1)
- Exposure Risk (1)
- Fibroblasten (1)
- GPCR (1)
- Genetics (1)
- Genexpression <Molekulargenetik> (1)
- Geschlechtsbestimmung (1)
- Geschlechtsdifferenzierung (1)
- HECT Ligase (1)
- HUWE1 (1)
- Holobiont (1)
- Human atrial stromal cells (1)
- Immune Escape (1)
- Interaktionen (1)
- Japankärpfling (1)
- K-Ras (1)
- Kryptolebias marmoratus (1)
- Landschaftsstruktur (1)
- Lungenkrebs (1)
- MAP-Kinase (1)
- MICA (1)
- MICB (1)
- MITE (1)
- MMB (1)
- Mcl-1 (1)
- Medaka (1)
- Medicine (1)
- Megalobrama amblycephala (1)
- Megaponera analis (1)
- Meiose (1)
- Meiosis (1)
- Melanom (1)
- Meliponini (1)
- Merkel cell carcinoma (1)
- Merkel-Zellkarzinom (1)
- Metabolic pathways (1)
- Mikrobiota (1)
- Mitose (1)
- Molecular Biophysics (1)
- Molekulare Hybridisierung (1)
- Monozytendifferenzierung (1)
- N-terminal domain (1)
- NAD (1)
- NADPH oxidase (1)
- NADPH-Oxidase (1)
- NFATc1 (1)
- Neisseria gonorrhoeae (1)
- Neisseria meningitidis (1)
- Neuromuscular junctions (1)
- Next-Generation Sequenzierung (1)
- Nicht-kleinzelliges Bronchialkarzinom (NSCLC) (1)
- Nährboden (1)
- OGEE v2 (1)
- Oilseed Rape (1)
- Oxidativer Stress (1)
- P14ARF (1)
- PDE4d (1)
- PER (1)
- Pipecolinsäurederivate (1)
- Pollen (1)
- Predictive toxicology (1)
- Produktivität (1)
- RNA in situ hybridization (1)
- RNA interference (1)
- Resource Use (1)
- Retinoesäure (1)
- Retinoic acid (1)
- Saccharomyces cerevisiae (1)
- Sammeldistanzen (1)
- Senescence (1)
- Septins (1)
- Sex determination (1)
- Staphylococcus aureus (1)
- Stoffwechsel (1)
- Structural Biology (1)
- Synapse (1)
- Synapses (1)
- Synaptic vesicles (1)
- Systembiologie (1)
- Systembiologische Analysen (1)
- T cells (1)
- T-cadherin (1)
- TAMs (1)
- TP53 (1)
- Telomer (1)
- Toxicity (1)
- Transcriptional control (1)
- Tumor Immunology (1)
- Ubiquitin (1)
- Vaccinia virus (1)
- Vesicles (1)
- Weinrebe (1)
- Wilms tumour (1)
- Wurzelhalsgalle (1)
- X-Ray Chrystallography (1)
- Zea mays (1)
- Zebrafish (1)
- Zellkultur (1)
- Zytokinine (Pflanzenpathogene) (1)
- active zone (1)
- acute myeloid leukaemia (1)
- adhesion GPCR (1)
- agroecology (1)
- alcohol tolerance (1)
- alkaloids (1)
- alu elements (1)
- anaplasia (1)
- angiotensin II type 1a receptor (1)
- antibiotics (1)
- antimicrobials (1)
- bakterielle Flora (1)
- bees (1)
- behavior (1)
- behavioural ecology (1)
- biogenic amines (1)
- bioinformatics (1)
- biomarker (1)
- biomaterials (1)
- biophysics (1)
- blood (1)
- blood platelets (1)
- bone (1)
- brain (1)
- brain development (1)
- breast cancer (1)
- cadherin-13 (CDH13) (1)
- cancer (1)
- cancer treatment (1)
- cash crops (1)
- catabolism (1)
- cell binding (1)
- cell cycle (1)
- cell cycle and cell division (1)
- cell proliferation (1)
- cell-autonomous defense (1)
- cellular function (1)
- cellular stress (1)
- central clocks (1)
- central complex (1)
- ceramide analogs (1)
- cholera (1)
- chromatin remodeling (1)
- chronobiology (1)
- circadian clock (1)
- circadian mechanisms (1)
- clinical genetics (1)
- cloning (1)
- cognition (1)
- colorectal carcinoma (1)
- compound conditioning (1)
- confocal laser scanning microscopy (1)
- confocal-microscopy based automated quantification (1)
- cotton (1)
- crotonase (1)
- cryptic (1)
- cytokinesis (1)
- cytotoxic T cells (1)
- dCIRL (1)
- damped circadian clock (1)
- decision-making (1)
- deformed wing virus (1)
- dehydrogenase (1)
- desert ants (1)
- deubiquitinase (1)
- developmental differentiation (1)
- differential olfactory conditioning (1)
- division of labor (1)
- dorsal raphe (1)
- dunce (1)
- eEF1A1 (1)
- eclosion (1)
- ecological genetics (1)
- ecosystem services (1)
- endophyte (1)
- epigenetics (1)
- eukaryota (1)
- evolutionary genetics (1)
- evolutionary response (1)
- fetal cord blood (1)
- fetal programming (1)
- floral resource distribution (1)
- fluorescence imaging (1)
- forager (1)
- foraging behaviour (1)
- foraging distances (1)
- functional complementarity (1)
- functional redundancy (1)
- fungal endophytes of grasses (1)
- fungal infection (1)
- fungal physiology (1)
- fungal structure (1)
- gambiense (1)
- gene essentiality database (1)
- gene expression (1)
- gene regulation (1)
- genetic loci (1)
- genome collection (1)
- genome integrity (1)
- genome sequencing (1)
- genomic libraries (1)
- genomic sequence (1)
- genomics research (1)
- gestational diabetes mellitus (1)
- glioblastoma multiforme (1)
- glycoprotein Ib (1)
- glycosylation (1)
- gonococcal (1)
- gonococcal infection (1)
- grass (1)
- gut microflora (1)
- habitat information (1)
- halbnatürliche Habitate (1)
- hangover (1)
- herbivorous diet (1)
- herpes virus (1)
- high throughput (1)
- high-throughput screening (1)
- histones (1)
- honey bees (1)
- honeybees (1)
- human cancer cell lines (1)
- humanized mice (1)
- hyperexpression techniques (1)
- ichthyology (1)
- image analysis (1)
- imaging the immune system (1)
- immune cells (1)
- immune evasion (1)
- infectious diseases (1)
- infectious-disease diagnostics (1)
- infrared radiation (1)
- insect (1)
- insulin treatment (1)
- intermuscular bone (1)
- kinesin (1)
- landscape ecology (1)
- landscape structure (1)
- leaves (1)
- light pulses (1)
- lipids (1)
- look-back behavior (1)
- lymphocyte activation (1)
- macrophages (1)
- mass spectrometry (1)
- mating success (1)
- mechanobiology (1)
- mechanosensing (1)
- mechanotransduction (1)
- media design (1)
- megakaryocytes (1)
- membrane receptor signaling (1)
- messenger RNA (1)
- metabotropic signalling (1)
- metagenomics (1)
- miRNA-Detektion (1)
- miRNS (1)
- microRNAs (1)
- microRNA–target interaction (1)
- microbiome (1)
- microswimmer (1)
- migration (1)
- mitochondria (1)
- mitotic gene expression (1)
- molecular diagnostics (1)
- molecular neuroscience (1)
- molting (1)
- monocyte (1)
- monocyte differentiation (1)
- morphometry (1)
- multiple myeloma (1)
- mushroom body (1)
- natural killer cells (1)
- neisseria meningitidis (1)
- nephroblastoma (1)
- nesting habit (1)
- networks (1)
- neuroanatomy (1)
- neurodevelopment (1)
- neuronal development (1)
- neuropeptide pathway (1)
- next-generation sequencing (1)
- none (1)
- nurse bee (1)
- nutrition (1)
- nutritional ecology (1)
- octopamine (1)
- optogenetics (1)
- orientation (1)
- osteocytes (1)
- oxaliplatin (1)
- oxidative stress (1)
- pancreatic cancer (1)
- parasitic cell cycles (1)
- parasitic diseases (1)
- pattern recognition receptors (1)
- perception (1)
- peripheral clocks (1)
- plant defense (1)
- plant-insect interactions (1)
- plant–insect interactions (1)
- point-of-care (1)
- pollination (1)
- pollinator decline (1)
- population structure (1)
- prefrontal cortex (1)
- proGenomes (1)
- proboscis extension reflex (1)
- product qualitymodulation (1)
- prokaryotic clade (1)
- prokaryotic subspecies (1)
- protein structure (1)
- proteins (1)
- proteomes (1)
- psychiatric disorders (1)
- quality control (1)
- radial glia (1)
- reactivating p53 and inducing tumor apoptosis (RITA) (1)
- recruitment (1)
- regulation (1)
- regulatory T cells (1)
- regulatory circuit downstream (1)
- relA (1)
- replication (1)
- reproductive asynchrony (1)
- rescue behavior (1)
- resin (1)
- retinal development (1)
- sacbrood virus (1)
- season (1)
- semi-natural habitat (1)
- sensory physiology (1)
- sept3 (1)
- sept5a (1)
- sept5b (1)
- septin (1)
- sequence assembly tools (1)
- serotonin (1)
- sesame (1)
- sex-specific mortality (1)
- sexual dimorphism in timing (1)
- signal transduction (1)
- signaling (1)
- sky-compass pathway (1)
- sleeping sickness (1)
- small interfering RNAs (1)
- social immunity (1)
- sphingolipids (1)
- sphingomyelinase (1)
- spider (1)
- spidroin (1)
- string database (1)
- stringent response (1)
- structural biology (1)
- substrate channeling (1)
- super-resolution microscopy (1)
- synaptic vesicle tethering (1)
- systems biology (1)
- taxonomic description (1)
- telomere-binding protein (1)
- tetracyclines (1)
- therapeutic strategy (1)
- throat (1)
- transcriptome (1)
- treatment (1)
- tsetse fly (1)
- tumour heterogeneity (1)
- vaccinia virus (1)
- vector navigation (1)
- video recording (1)
- virulenceregulatory evolution (1)
- visual orientation (1)
- whole genome (1)
- yellow fluorescent protein (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (82) (remove)
Sonstige beteiligte Institutionen
Staphylococcus aureus (S. aureus) infections are a major clinical problem and range from mild skin and soft-tissue infections to severe and even lethal infections such as pneumonia, endocarditis, sepsis, osteomyelitis, and toxic shock syndrome. Toxins that are released from S. aureus mediate many of these effects. Here, we aimed to identify molecular mechanisms how α-toxin, a major S. aureus toxin, induces inflammation. Methods: Macrophages were isolated from the bone marrow of wildtype and acid sphingomyelinase-deficient mice, stimulated with S. aureus α-toxin and activation of the acid sphingomyelinase was quantified. The subcellular formation of ceramides was determined by confocal microscopy. Release of cathepsins from lysosomes, activation of inflammasome proteins and formation of Interleukin-1β (IL-1β) and Tumor Necrosis Factor-α (TNF-α) were analyzed by western blotting, confocal microscopy and ELISA. Results: We demonstrate that S. aureus α-toxin activates the acid sphingomyelinase in ex vivo macrophages and triggers a release of ceramides. Ceramides induced by S. aureus α-toxin localize to lysosomes and mediate a release of cathepsin B and D from lysosomes into the cytoplasm. Cytosolic cathepsin B forms a complex with Nlrc4. Treatment of macrophages with α-toxin induces the formation of IL-1β and TNF-α. These events are reduced or abrogated, respectively, in cells lacking the acid sphingomyelinase and upon treatment of macrophages with amitriptyline, a functional inhibitor of acid sphingomyelinase. Pharmacological inhibition of cathepsin B prevented activation of the inflammasome measured as release of IL-1β, while the formation of TNF-α was independent of cathepsin B. Conclusion: We demonstrate a novel mechanism how bacterial toxins activate the inflammasome and mediate the formation and release of cytokines: S. aureus α-toxin triggers an activation of the acid sphingomyelinase and a release of ceramides resulting in the release of lysosomal cathepsin B and formation of pro-inflammatory cytokines.