Refine
Has Fulltext
- yes (35) (remove)
Is part of the Bibliography
- yes (35) (remove)
Year of publication
Document Type
- Journal article (18)
- Doctoral Thesis (16)
- Preprint (1)
Keywords
- fluorescence (35) (remove)
Institute
- Institut für Organische Chemie (14)
- Institut für Anorganische Chemie (12)
- Theodor-Boveri-Institut für Biowissenschaften (4)
- Institut für Pharmakologie und Toxikologie (2)
- Institut für Physikalische und Theoretische Chemie (2)
- Fakultät für Chemie und Pharmazie (1)
- Institut für Pharmazie und Lebensmittelchemie (1)
- Julius-von-Sachs-Institut für Biowissenschaften (1)
- Lehrstuhl für Silicatchemie (1)
- Medizinische Fakultät (1)
Sonstige beteiligte Institutionen
- Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany (1)
- Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan (1)
- International Max Planck Research School Molecular Biology, University of Göttingen, Germany (1)
- Université de Bordeaux, Bordeaux, France (1)
EU-Project number / Contract (GA) number
- 682586 (1)
Large Stokes shift (LSS) fluorescent proteins (FPs) exploit excited state proton transfer pathways to enable fluorescence emission from the phenolate intermediate of their internal 4 hydroxybenzylidene imidazolone (HBI) chromophore. An RNA aptamer named Chili mimics LSS FPs by inducing highly Stokes-shifted emission from several new green and red HBI analogs that are non-fluorescent when free in solution. The ligands are bound by the RNA in their protonated phenol form and feature a cationic aromatic side chain for increased RNA affinity and reduced magnesium dependence. In combination with oxidative functional-ization at the C2 position of the imidazolone, this strategy yielded DMHBO\(^+\), which binds to the Chili aptamer with a low-nanomolar K\(_D\). Because of its highly red-shifted fluorescence emission at 592 nm, the Chili–DMHBO\(^+\) complex is an ideal fluorescence donor for Förster resonance energy transfer (FRET) to the rhodamine dye Atto 590 and will therefore find applications in FRET-based analytical RNA systems.