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- 2021 (5) (entfernen)
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- Englisch (5)
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- RNA (2)
- Biochemistry (1)
- Chili RNA Aptamer (1)
- Co-Crystal Structures of Chili RNA (1)
- Cryoelectron Microscopy (1)
- Cryoelectron microscopy (1)
- Deoxyribozymes (1)
- Epitranscriptomics (1)
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- Molecular mechanism (1)
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- Department of Cellular Biochemistry, University Medical Center Göttingen (1)
- Georg August University School of Science (1)
- Institute of Cancer Research (ICR) London (1)
- Max Planck Institute for Biophysical Chemistry (1)
- Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen (1)
- Max Planck Institute for Biophysical Chemistry, Research Group Structure and Function of Molecular Machines, Göttingen (1)
- Max-Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen (1)
- University Medical Center Göttingen, Department of Cellular Biochemistry, Göttingen (1)
EU-Projektnummer / Contract (GA) number
- 682586 (5) (entfernen)
Deoxyribozymes are emerging as modification-specific endonucleases for the analysis of epigenetic RNA modifications. Here, we report RNA-cleaving deoxyribozymes that differentially respond to the presence of natural methylated cytidines, 3-methylcytidine (m\(^3\)C), N\(^4\)-methylcytidine (m\(^4\)C), and 5-methylcytidine (m\(^5\)C), respectively. Using in vitro selection, we found several DNA catalysts, which are selectively activated by only one of the three cytidine isomers, and display 10- to 30-fold accelerated cleavage of their target m\(^3\)C-, m\(^4\)C- or m\(^5\)C-modified RNA. An additional deoxyribozyme is strongly inhibited by any of the three methylcytidines, but effectively cleaves unmodified RNA. The mXC-detecting deoxyribozymes are programmable for the interrogation of natural RNAs of interest, as demonstrated for human mitochondrial tRNAs containing known m\(^3\)C and m\(^5\)C sites. The results underline the potential of synthetic functional DNA to shape highly selective active sites.