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- age (12)
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- case report (12)
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- dynamics (12)
- endothelium (12)
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- lymphocytes (12)
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- measles (12)
- metaanalysis (12)
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- optimization (12)
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- pollen (12)
- polymers (12)
- rats (12)
- recombination (12)
- reliability (12)
- screening (12)
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- systematic review (12)
- tinnitus (12)
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- trabeculectomy (12)
- translation (12)
- ubiquitin (12)
- ultrasound (12)
- vitamin D (12)
- vorsprachliche Entwicklung (12)
- zebrafish (12)
- ++ (11)
- 3D (11)
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- Arabidopsis (11)
- B-Zelle (11)
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- Candida (11)
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- Diboren (11)
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- Dynamik (11)
- EGFR (11)
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- Enzym (11)
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- Model (11)
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- Supraleitung (11)
- Symbiose (11)
- T cell (11)
- Training (11)
- Tuberkelbakterium (11)
- Tumor-Nekrose-Faktor <alpha> (11)
- USA (11)
- Ubiquitin (11)
- Vasodilatator-stimuliertes Phosphoprotein (11)
- Wahrnehmung (11)
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- Zelltod (11)
- adhesion (11)
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- antibody (11)
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- mechanism (11)
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- next generation sequencing (11)
- nuclear envelope (11)
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- periodontitis (11)
- plasticity (11)
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- proteomics (11)
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- relapse (11)
- review (11)
- safety (11)
- synaptic plasticity (11)
- topological insulator (11)
- transport (11)
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- Alkohol (10)
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- Children (10)
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- Coronaviren (10)
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- Diamant (10)
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- Dotierung (10)
- Echokardiographie (10)
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- Elektrochemie (10)
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- Ereigniskorreliertes Potenzial (10)
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- Frau (10)
- Funktionalisierung <Chemie> (10)
- Förderung (10)
- Geldpolitik (10)
- Glatter Krallenfrosch (10)
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- Impfstoff (10)
- Induzierte pluripotente Stammzelle (10)
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- Kniegelenk (10)
- Kohlenstoff (10)
- Komplikation (10)
- Kultur (10)
- LASP1 (10)
- LC-MS (10)
- Ladungstransfer (10)
- Ligand (10)
- Lokalisation (10)
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- Niger (10)
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- Pain (10)
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- Parasit (10)
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- Pathogenität (10)
- Platelets (10)
- Plattenepithelkarzinom (10)
- Prevalence (10)
- Prostaglandine (10)
- Präfrontaler Cortex (10)
- Pädagogik (10)
- Qualitätskontrolle (10)
- RNA interference (10)
- RNA-seq (10)
- RNS-Spleißen (10)
- Radiotherapy (10)
- Remote Sensing (10)
- Rhodium (10)
- Rituximab (10)
- SAR (10)
- Schilddrüse (10)
- Schultergelenk (10)
- Silaanaloga (10)
- Silber (10)
- Silicon (10)
- South Africa (10)
- Sphingolipide (10)
- Sprachverstehen (10)
- Starke Kopplung (10)
- Stickstoffmonoxid-Synthase (10)
- Stressreaktion (10)
- Stroke (10)
- Struktur (10)
- T-Lymphozyten (10)
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- Tagesrhythmus (10)
- Totalsynthese (10)
- Toxizität (10)
- Transplantat-Wirt-Reaktion (10)
- Treg (10)
- Trypanosomen (10)
- Vitamin D (10)
- Vor- und Frühgeschichte (10)
- Vorschulkind (10)
- Wachstum (10)
- Zebrabärbling (10)
- amino acids (10)
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- bariatric surgery (10)
- biosynthesis (10)
- boranes (10)
- cartilage (10)
- chemokines (10)
- circadian rhythms (10)
- clinical trial (10)
- comparison (10)
- complex (10)
- coronary artery disease (10)
- decision-making (10)
- density functional calculations (10)
- eNOS (10)
- emotion regulation (10)
- fatigue (10)
- fear (10)
- genome-wide association (10)
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- immunology (10)
- immunomodulation (10)
- injury (10)
- insect (10)
- knockout (10)
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- phenotype (10)
- quality assurance (10)
- regulatorische T-Zellen (10)
- remodeling (10)
- senescence (10)
- silicon (10)
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- stem cell transplantation (10)
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- Östrogene (10)
- 3 (9)
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- jet energy scale (8)
- juvenile idiopathic arthritis (8)
- kinematic alignment (8)
- latency (8)
- mHealth (8)
- macrophage (8)
- major depression (8)
- meta-analysis (8)
- methylphenidate (8)
- microenvironment (8)
- microstructure (8)
- monocyte (8)
- mutualism (8)
- neurogenesis (8)
- optical spectroscopy (8)
- optimal control (8)
- organic solar cells (8)
- organoids (8)
- pediatrics (8)
- permafrost (8)
- personality (8)
- pharmacology (8)
- photocatalysis (8)
- phylogeny (8)
- polycyclic aromatic hydrocarbons (8)
- preconditioning (8)
- presence (8)
- progression (8)
- protease (8)
- pulmonary hypertension (8)
- quantum dots (8)
- questionnaire (8)
- radiation (8)
- radicals (8)
- radioligand therapy (8)
- receptors (8)
- scaffold (8)
- secondary structure (8)
- secretion (8)
- selection (8)
- semiconductor (8)
- shoulder (8)
- sleep (8)
- social insects (8)
- sodium (8)
- species richness (8)
- spintronics (8)
- staphylococcus aureus (8)
- structure (8)
- subthalamic nucleus (8)
- susceptibility (8)
- targeted therapy (8)
- thymus (8)
- tool (8)
- 3D tissue model (7)
- 53BP1 (7)
- Adsorption (7)
- Adulte Stammzelle (7)
- Africa (7)
- Aktin (7)
- Aktivierung (7)
- Aktivierung <Physiologie> (7)
- Akute myeloische Leukämie (7)
- Akutes Nierenversagen (7)
- Alltagskultur (7)
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- Behandlung (7)
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- Biographie (7)
- Blattschneiderameisen (7)
- Blut (7)
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- Bordetella (7)
- Borylenkomplexe (7)
- CMV (7)
- CNS (7)
- Calciumkanal (7)
- Catecholmethyltransferase <Catechol-0-Methyltransferase> (7)
- Charakterisierung (7)
- Charcot-Marie-Tooth (7)
- Chronobiologie (7)
- Chrysomelidae (7)
- Cisplatin (7)
- Cytoskeleton (7)
- DHEA (7)
- DNS-Doppelstrangbruch (7)
- Degradation (7)
- Deutsch als Fremdsprache (7)
- Differentielle Genexpression (7)
- Dopamine (7)
- Durchblutung (7)
- Durchflusszytometrie (7)
- Dysgnathie (7)
- E-Learning (7)
- ELISA (7)
- ELISPOT (7)
- EMG (7)
- ERK1/2 (7)
- ERN (7)
- Einwandige Kohlenstoff-Nanoröhre (7)
- Eisen (7)
- Elektrokardiogramm (7)
- Embryo (7)
- Endoprothetik (7)
- Ethik (7)
- Europa (7)
- Exziton-Polariton (7)
- FISH (7)
- Fahrsimulator (7)
- Festkörperoberfläche (7)
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- Fluoreszenz-in-situ-Hybridisierung (7)
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- Fortpflanzung (7)
- Fraktur (7)
- Freundschaft (7)
- GABA (7)
- Gallium (7)
- Gehirn-Computer-Schnittstelle (7)
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- LEED (7)
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- Promotor (7)
- Protein-Tyrosin-Kinasen (7)
- Proteinkinase C (7)
- Proteomanalyse (7)
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- Qualitätssicherung (7)
- Quantenwell (7)
- REMPI (7)
- Raman (7)
- Rat (7)
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- Roman (7)
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- SMA (7)
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- Wilms tumor (7)
- Wolfram (7)
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- absorption (7)
- acute ischemic stroke (7)
- acute kidney injury (7)
- acute myeloid leukemia (7)
- adaptation (7)
- adipose tissue (7)
- adolescent (7)
- adult neurogenesis (7)
- adults (7)
- algorithm (7)
- allogeneic stem cell transplantation (7)
- anemia (7)
- animal behavior (7)
- antibacterial activity (7)
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- antidepressant (7)
- arabidopsis thaliana (7)
- behaviour (7)
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- bone mineral density (7)
- brain development (7)
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- butyrate (7)
- caffeine (7)
- capillary electrophoresis (7)
- carbon dioxide (7)
- carcinoma (7)
- cell membranes (7)
- change detection (7)
- chemokine receptor (7)
- classical conditioning (7)
- click chemistry (7)
- cognition (7)
- cognitive control (7)
- cognitive decline (7)
- comparative genomics (7)
- complexes (7)
- complexity (7)
- crystallization (7)
- cytokinesis (7)
- cytotoxic T cells (7)
- degeneration (7)
- degradation (7)
- diborynes (7)
- diffusion (7)
- distribution (7)
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- drug (7)
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- efficacy (7)
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- genes (7)
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- guard cell (7)
- heterocycles (7)
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- hypophosphatasia (7)
- immersion (7)
- immunofluorescence (7)
- immunoprecipitation (7)
- inflammatory bowel disease (7)
- kinase (7)
- knee (7)
- knockout mice (7)
- kolorektales Karzinom (7)
- learning and memory (7)
- lithium (7)
- localization (7)
- luciferase (7)
- melt electrospinning writing (7)
- membrane (7)
- meningitis (7)
- methionine (7)
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- minimally invasive (7)
- modeling (7)
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- motoneuron (7)
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- pemphigus (7)
- photoinduced electron transfer (7)
- plasmodium falciparum (7)
- population (7)
- potassium (7)
- pre-speech development (7)
- precision medicine (7)
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- prospective (7)
- psoriasis (7)
- pulse shaping (7)
- quantum dynamics (7)
- radioiodine therapy (7)
- random forest (7)
- recognition (7)
- recovery (7)
- recruitment (7)
- reperfusion (7)
- resilience (7)
- responses (7)
- retina (7)
- risk factor (7)
- rotator cuff (7)
- sRNA (7)
- salt stress (7)
- secondary prevention (7)
- segmentation (7)
- semiconductors (7)
- serotonin transporter (7)
- sex chromosomes (7)
- shear stress (7)
- signaltransduction (7)
- small RNA (7)
- smoking (7)
- social anxiety (7)
- social cognition (7)
- speech audiometry (7)
- superconductivity (7)
- target (7)
- technology (7)
- telomeres (7)
- thermodynamics (7)
- total hip arthroplasty (7)
- transcriptomics (7)
- trial (7)
- variability (7)
- vestibular schwannoma (7)
- virus (7)
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- wearable (7)
- Übergangsmetalloxide (7)
- Übergewicht (7)
- - (6)
- 18F-FDG (6)
- 2 (6)
- ABA (6)
- ACC (6)
- AMPK (6)
- ANP (6)
- ATLAS <Teilchendetektor> (6)
- Abwehrreaktion (6)
- AdS-CFT-Korrespondenz (6)
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- Fragmentation (6)
- Frankreich (6)
- Französisch (6)
- Funktionelle NMR-Tomographie (6)
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- GPVI (6)
- GaAs (6)
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- Imaging (6)
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- Interview (6)
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- Juvenile idiopathische Arthritis (6)
- Kation (6)
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- Learning (6)
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- acid sphingomyelinase (6)
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- adenosine (6)
- adsorption (6)
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- altitudinal gradient (6)
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- larvae (5)
- late enhancement (5)
- lipid rafts (5)
- long-term outcome (5)
- longitudinal study (5)
- loss of heterozygosity (5)
- loudness (5)
- magnetism (5)
- masked priming (5)
- mechanical thrombectomy (5)
- medical education (5)
- medical rehabilitation (5)
- megakaryocyte (5)
- mental disorders (5)
- mesenchymal stem cell (5)
- metabolic syndrome (5)
- microglomeruli (5)
- microsatellite instability (5)
- mineralization (5)
- minimal invasiv (5)
- mobility (5)
- modelling (5)
- molecular medicine (5)
- motoneurons (5)
- motor control (5)
- movement disorders (5)
- movement ecology (5)
- music (5)
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- nano (5)
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- naphthylisoquinoline alkaloids (5)
- natural disturbance (5)
- neisseria meningitidis (5)
- nephroblastoma (5)
- network (5)
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- neuromelanin (5)
- neuronal plasticity (5)
- neuropeptides (5)
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- neuroscience (5)
- neurotrophic factor (5)
- newborn hearing screening (5)
- nickel (5)
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- norepinephrine (5)
- nucleolus (5)
- nutrients (5)
- object detection (5)
- oncolysis (5)
- organic semiconductor (5)
- organic synthesis (5)
- organische Halbleiter (5)
- osteoblasts (5)
- parasitic diseases (5)
- parathyroid hormone (5)
- patch-clamp (5)
- pathogenicity (5)
- peptide (5)
- persistence (5)
- pharmacokinetic (5)
- phase variation (5)
- phylogenetic analysis (5)
- plant-insect interactions (5)
- plants (5)
- plasma cells (5)
- polyneuropathy (5)
- polyomavirus (5)
- population pharmacokinetics (5)
- porin (5)
- predictors (5)
- promoter (5)
- prostate-specific membrane antigen (5)
- protein binding (5)
- protein expression (5)
- psychiatry (5)
- psychological distress (5)
- public health (5)
- pyrolysis (5)
- quality (5)
- quantum optics (5)
- radial (5)
- reactive intermediates (5)
- receptor tyrosine kinase (5)
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- refractory (5)
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- resin (5)
- resolution (5)
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- retrospective (5)
- revision (5)
- reward (5)
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- ruthenium (5)
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- self (5)
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- sevoflurane (5)
- sex differences (5)
- shock (5)
- single-crystal X-ray study (5)
- skeletal muscle (5)
- soil (5)
- somatostatin receptor (5)
- soziale Insekten (5)
- soziale Kompetenz (5)
- specificity (5)
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- spin (5)
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- splicing (5)
- stability (5)
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- structural elucidation (5)
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- subtypes (5)
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- tomography (5)
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- transgenic mice (5)
- transporter (5)
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- variants (5)
- vascularization (5)
- vasculitis (5)
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- virulence factors (5)
- visualization (5)
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- well-being (5)
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- winter wheat (5)
- women (5)
- xx (5)
- zwitterionic (5)
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- Ökonometrie (5)
- Überlebensrate (5)
- γ-H2AX (5)
- --- (4)
- 3D Printing (4)
- 3D cell culture (4)
- 3D reconstruction (4)
- 5-Fluorouracil (4)
- ACL (4)
- AGN (4)
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- APOBEC3G (4)
- ASSR (4)
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- Ablösung (4)
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- Charged Aerosol Detection (4)
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- DNS-Bindung (4)
- DRG (4)
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- Diffusionsverfahren <Halbleitertechnologie> (4)
- Dimension 2 (4)
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- Friedrich (4)
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- G-Protein gekoppelte Rezeptor (4)
- G-Protein gekoppelter Rezeptor (4)
- GC-A (4)
- GVHD (4)
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- Theodor-Boveri-Institut für Biowissenschaften (2211)
- Graduate School of Life Sciences (982)
- Physikalisches Institut (781)
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- Universität - Fakultätsübergreifend (483)
Schriftenreihe
- Cultural Animal Studies, Band 3 (2)
- Spezielle Didaktik der Sportarten (2)
- Alter Orient und Altes Testament : Sonderreihe Veröffentlichungen zur Kultur und Geschichte des Alten Orients ; 3 (1)
- Berichte aus der Informatik (1)
- Deuterocanonical and Cognate Literature Studies (1)
- Deuterocanonical and Cognate Literature Yearbook (1)
- European Journal of Clinical Nutrition ; 70 (1)
- Forum Junge Romanistik 18 (1)
- Grundzüge ; 45 (1)
- Journal of Neuroimaging; Volume 28 (1)
Sonstige beteiligte Institutionen
- Johns Hopkins School of Medicine (18)
- Fraunhofer-Institut für Silicatforschung ISC (8)
- IZKF Nachwuchsgruppe Geweberegeneration für muskuloskelettale Erkrankungen (7)
- Akademie der Wissenschaften und der Literatur, Mainz (6)
- DFG Forschungsgruppe 2757 / Lokale Selbstregelungen im Kontext schwacher Staatlichkeit in Antike und Moderne (LoSAM) (6)
- Clinical Trial Center (CTC) / Zentrale für Klinische Studien Würzburg (ZKSW) (5)
- Helmholtz Institute for RNA-based Infection Research (HIRI) (5)
- Johns Hopkins University School of Medicine (5)
- Universität Leipzig (5)
- Universitätsklinikum Würzburg (5)
ResearcherID
- B-1911-2015 (1)
- B-4606-2017 (1)
- C-2593-2016 (1)
- D-1221-2009 (1)
- D-1250-2010 (1)
- D-3057-2014 (1)
- I-5818-2014 (1)
- J-8841-2015 (1)
- M-1240-2017 (1)
- N-2030-2015 (1)
Background
Medical resource management can be improved by assessing the likelihood of prolonged length of stay (LOS) for head and neck cancer surgery patients. The objective of this study was to develop predictive models that could be used to determine whether a patient's LOS after cancer surgery falls within the normal range of the cohort.
Methods
We conducted a retrospective analysis of a dataset consisting of 300 consecutive patients who underwent head and neck cancer surgery between 2017 and 2022 at a single university medical center. Prolonged LOS was defined as LOS exceeding the 75th percentile of the cohort. Feature importance analysis was performed to evaluate the most important predictors for prolonged LOS. We then constructed 7 machine learning and deep learning algorithms for the prediction modeling of prolonged LOS.
Results
The algorithms reached accuracy values of 75.40 (radial basis function neural network) to 97.92 (Random Trees) for the training set and 64.90 (multilayer perceptron neural network) to 84.14 (Random Trees) for the testing set. The leading parameters predicting prolonged LOS were operation time, ischemia time, the graft used, the ASA score, the intensive care stay, and the pathological stages. The results revealed that patients who had a higher number of harvested lymph nodes (LN) had a lower probability of recurrence but also a greater LOS. However, patients with prolonged LOS were also at greater risk of recurrence, particularly when fewer (LN) were extracted. Further, LOS was more strongly correlated with the overall number of extracted lymph nodes than with the number of positive lymph nodes or the ratio of positive to overall extracted lymph nodes, indicating that particularly unnecessary lymph node extraction might be associated with prolonged LOS.
Conclusions
The results emphasize the need for a closer follow-up of patients who experience prolonged LOS. Prospective trials are warranted to validate the present results.
Learning about informal fallacies and the detection of fake news: an experimental intervention
(2023)
The philosophical concept of informal fallacies–arguments that fail to provide sufficient support for a claim–is introduced and connected to the topic of fake news detection. We assumed that the ability to identify informal fallacies can be trained and that this ability enables individuals to better distinguish between fake news and real news. We tested these assumptions in a two-group between-participants experiment (N = 116). The two groups participated in a 30-minute-long text-based learning intervention: either about informal fallacies or about fake news. Learning about informal fallacies enhanced participants’ ability to identify fallacious arguments one week later. Furthermore, the ability to identify fallacious arguments was associated with a better discernment between real news and fake news. Participants in the informal fallacy intervention group and the fake news intervention group performed equally well on the news discernment task. The contribution of (identifying) informal fallacies for research and practice is discussed.
Highlights
• Loss of DNAJC19's DnaJ domain disrupts cardiac mitochondrial structure, leading to abnormal cristae formation in iPSC-CMs.
• Impaired mitochondrial structures lead to an increased mitochondrial respiration, ROS and an elevated membrane potential.
• Mutant iPSC-CMs show sarcomere dysfunction and a trend to more arrhythmias, resembling DCMA-associated cardiomyopathy.
Background
Dilated cardiomyopathy with ataxia (DCMA) is an autosomal recessive disorder arising from truncating mutations in DNAJC19, which encodes an inner mitochondrial membrane protein. Clinical features include an early onset, often life-threatening, cardiomyopathy associated with other metabolic features. Here, we aim to understand the metabolic and pathophysiological mechanisms of mutant DNAJC19 for the development of cardiomyopathy.
Methods
We generated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) of two affected siblings with DCMA and a gene-edited truncation variant (tv) of DNAJC19 which all lack the conserved DnaJ interaction domain. The mutant iPSC-CMs and their respective control cells were subjected to various analyses, including assessments of morphology, metabolic function, and physiological consequences such as Ca\(^{2+}\) kinetics, contractility, and arrhythmic potential. Validation of respiration analysis was done in a gene-edited HeLa cell line (DNAJC19tv\(_{HeLa}\)).
Results
Structural analyses revealed mitochondrial fragmentation and abnormal cristae formation associated with an overall reduced mitochondrial protein expression in mutant iPSC-CMs. Morphological alterations were associated with higher oxygen consumption rates (OCRs) in all three mutant iPSC-CMs, indicating higher electron transport chain activity to meet cellular ATP demands. Additionally, increased extracellular acidification rates suggested an increase in overall metabolic flux, while radioactive tracer uptake studies revealed decreased fatty acid uptake and utilization of glucose. Mutant iPSC-CMs also showed increased reactive oxygen species (ROS) and an elevated mitochondrial membrane potential. Increased mitochondrial respiration with pyruvate and malate as substrates was observed in mutant DNAJC19tv HeLa cells in addition to an upregulation of respiratory chain complexes, while cellular ATP-levels remain the same. Moreover, mitochondrial alterations were associated with increased beating frequencies, elevated diastolic Ca\(^{2+}\) concentrations, reduced sarcomere shortening and an increased beat-to-beat rate variability in mutant cell lines in response to β-adrenergic stimulation.
Conclusions
Loss of the DnaJ domain disturbs cardiac mitochondrial structure with abnormal cristae formation and leads to mitochondrial dysfunction, suggesting that DNAJC19 plays an essential role in mitochondrial morphogenesis and biogenesis. Moreover, increased mitochondrial respiration, altered substrate utilization, increased ROS production and abnormal Ca\(^{2+}\) kinetics provide insights into the pathogenesis of DCMA-related cardiomyopathy.
To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca\(^{2+}\). In our search for species-dependent functional TPC1 channel variants with different luminal Ca\(^{2+}\) sensitivity, we found in total three acidic residues present in Ca\(^{2+}\) sensor sites 2 and 3 of the Ca\(^{2+}\)-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca\(^{2+}\). When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca\(^{2+}\) sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca\(^{2+}\) sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.
Interleukin-4 (IL-4) plays a key role in atopic diseases. It coordinates T-helper cell differentiation to subtype 2, thereby directing defense toward humoral immunity. Together with Interleukin-13, IL-4 further induces immunoglobulin class switch to IgE. Antibodies of this type activate mast cells and basophilic and eosinophilic granulocytes, which release pro-inflammatory mediators accounting for the typical symptoms of atopic diseases. IL-4 and IL-13 are thus major targets for pharmaceutical intervention strategies to treat atopic diseases. Besides neutralizing antibodies against IL-4, IL-13, or its receptors, IL-4 antagonists can present valuable alternatives. Pitrakinra, an Escherichia coli-derived IL-4 antagonist, has been evaluated in clinical trials for asthma treatment in the past; however, deficits such as short serum lifetime and potential immunogenicity among others stopped further development. To overcome such deficits, PEGylation of therapeutically important proteins has been used to increase the lifetime and proteolytic stability. As an alternative, glycoengineering is an emerging strategy used to improve pharmacokinetics of protein therapeutics. In this study, we have established different strategies to attach glycan moieties to defined positions in IL-4. Different chemical attachment strategies employing thiol chemistry were used to attach a glucose molecule at amino acid position 121, thereby converting IL-4 into a highly effective antagonist. To enhance the proteolytic stability of this IL-4 antagonist, additional glycan structures were introduced by glycoengineering utilizing eucaryotic expression. IL-4 antagonists with a combination of chemical and biosynthetic glycoengineering could be useful as therapeutic alternatives to IL-4 neutralizing antibodies already used to treat atopic diseases.
Graphs provide a key means to model relationships between entities.
They consist of vertices representing the entities,
and edges representing relationships between pairs of entities.
To make people conceive the structure of a graph,
it is almost inevitable to visualize the graph.
We call such a visualization a graph drawing.
Moreover, we have a straight-line graph drawing
if each vertex is represented as a point
(or a small geometric object, e.g., a rectangle)
and each edge is represented as a line segment between its two vertices.
A polyline is a very simple straight-line graph drawing,
where the vertices form a sequence according to which the vertices are connected by edges.
An example of a polyline in practice is a GPS trajectory.
The underlying road network, in turn, can be modeled as a graph.
This book addresses problems that arise
when working with straight-line graph drawings and polylines.
In particular, we study algorithms
for recognizing certain graphs representable with line segments,
for generating straight-line graph drawings,
and for abstracting polylines.
In the first part, we first examine,
how and in which time we can decide
whether a given graph is a stick graph,
that is, whether its vertices can be represented as
vertical and horizontal line segments on a diagonal line,
which intersect if and only if there is an edge between them.
We then consider the visual complexity of graphs.
Specifically, we investigate, for certain classes of graphs,
how many line segments are necessary for any straight-line graph drawing,
and whether three (or more) different slopes of the line segments
are sufficient to draw all edges.
Last, we study the question,
how to assign (ordered) colors to the vertices of a graph
with both directed and undirected edges
such that no neighboring vertices get the same color
and colors are ascending along directed edges.
Here, the special property of the considered graph is
that the vertices can be represented as intervals
that overlap if and only if there is an edge between them.
The latter problem is motivated by an application
in automated drawing of cable plans with vertical and horizontal line segments,
which we cover in the second part.
We describe an algorithm that
gets the abstract description of a cable plan as input,
and generates a drawing that takes into account
the special properties of these cable plans,
like plugs and groups of wires.
We then experimentally evaluate the quality of the resulting drawings.
In the third part, we study the problem of abstracting (or simplifying)
a single polyline and a bundle of polylines.
In this problem, the objective is to remove as many vertices as possible from the given polyline(s)
while keeping each resulting polyline sufficiently similar to its original course
(according to a given similarity measure).
Recent studies link increased ozone (O\(_3\)) and carbon dioxide (CO\(_2\)) levels to alteration of plant performance and plant-herbivore interactions, but their interactive effects on plant-pollinator interactions are little understood. Extra floral nectaries (EFNs) are essential organs used by some plants for stimulating defense against herbivory and for the attraction of insect pollinators, e.g., bees. The factors driving the interactions between bees and plants regarding the visitation of bees to EFNs are poorly understood, especially in the face of global change driven by greenhouse gases. Here, we experimentally tested whether elevated levels of O\(_3\) and CO\(_2\) individually and interactively alter the emission of Volatile Organic Compound (VOC) profiles in the field bean plant (Vicia faba, L., Fabaceae), EFN nectar production and EFN visitation by the European orchard bee (Osmia cornuta, Latreille, Megachilidae). Our results showed that O\(_3\) alone had significant negative effects on the blends of VOCs emitted while the treatment with elevated CO\(_2\) alone did not differ from the control. Furthermore, as with O\(_3\) alone, the mixture of O\(_3\) and CO\(_2\) also had a significant difference in the VOCs’ profile. O\(_3\) exposure was also linked to reduced nectar volume and had a negative impact on EFN visitation by bees. Increased CO\(_2\) level, on the other hand, had a positive impact on bee visits. Our results add to the knowledge of the interactive effects of O\(_3\) and CO\(_2\) on plant volatiles emitted by Vicia faba and bee responses. As greenhouse gas levels continue to rise globally, it is important to take these findings into consideration to better prepare for changes in plant-insect interactions.
Idiopathic Parkinson’s disease (PD) is characterized by a progredient degeneration of the brain, starting at deep subcortical areas such as the dorsal motor nucleus of the glossopharyngeal and vagal nerves (DM) (stage 1), followed by the coeruleus–subcoeruleus complex; (stage 2), the substantia nigra (SN) (stage 3), the anteromedial temporal mesocortex (MC) (stage 4), high-order sensory association areas and prefrontal fields (HC) (stage 5) and finally first-order sensory association areas, premotor areas, as well as primary sensory and motor field (FC) (stage 6). Autoimmunity might play a role in PD pathogenesis. Here we analyzed whether anti-brain autoantibodies differentially recognize different human brain areas and identified autoantigens that correlate with the above-described dissemination of PD pathology in the brain. Brain tissue was obtained from deceased individuals with no history of neurological or psychiatric disease and no neuropathological abnormalities. Tissue homogenates from different brain regions (DM, SN, MC, HC, FC) were subjected to SDS-PAGE and Western blot. Blots were incubated with plasma samples from 30 PD patients and 30 control subjects and stained with anti-IgG antibodies to detect anti-brain autoantibodies. Signals were quantified. Prominent autoantigens were identified by 2D-gel-coupled mass spectrometry sequencing. Anti-brain autoantibodies are frequent and occur both in healthy controls and individuals with PD. Glial fibrillary acidic protein (GFAP) was identified as a prominent autoantigen recognized in all plasma samples. GFAP immunoreactivity was highest in DM areas and lowest in FC areas with no significant differences in anti-GFAP autoantibody titers between healthy controls and individuals with PD. The anti-GFAP autoimmunoreactivity of different brain areas correlates with the dissemination of histopathological neurodegeneration in PD. We hypothesize that GFAP autoantibodies are physiological but might be involved as a cofactor in PD pathogenesis secondary to a leakage of the blood–brain barrier.
Background
Labelled with lutetium-177, the urea-based small molecules PSMA I&T and PSMA-617 are the two agents most frequently used for radioligand therapy (RLT) in patients with advanced metastatic castration-resistant and prostate-specific membrane antigen (PSMA) expressing prostate cancer (mCRPC). In this matched-pair analysis, we aimed to compare the toxicity and efficacy of both agents for PSMA-directed RLT.
Materials and methods
A total of 110 mCRPC patients from two centres were accrued, 55 individuals treated with [\(^{177}\)Lu]Lu-PSMA I&T, and a matched cohort of 55 patients treated with [\(^{177}\)Lu]Lu-PSMA-617. Matching criteria included age at the first cycle, Gleason score, prostate-specific antigen (PSA) values, and previous taxane-based chemotherapy. Using common terminology criteria for adverse events (CTCAE v. 5.0), toxicity profiles were investigated (including bone marrow and renal toxicity). Overall survival (OS) between both groups was compared.
Results
Toxicity assessment revealed grade III anaemia in a single patient (1.8%) for [\(^{177}\)Lu]Lu-PSMA I&T and five (9.1%) for [\(^{177}\)Lu]Lu-PSMA-617. In addition, one (1.9%) grade III thrombopenia for [\(^{177}\)Lu]Lu-PSMA-617 was recorded. Apart from that, no other grade III/IV toxicities were present. A median OS of 12 months for patients treated with [\(^{177}\)Lu]Lu-PSMA I&T did not differ significantly when compared to patients treated with [\(^{177}\)Lu]Lu-PSMA-617 (median OS, 13 months; P = 0.89).
Conclusion
In this matched-pair analysis of patients receiving one of the two agents most frequently applied for PSMA RLT, the rate of clinically relevant toxicities was low for both compounds. In addition, no relevant differences for OS were observed.
Natural DNA storage allows cellular differentiation, evolution, the growth of our children and controls all our ecosystems. Here, we discuss the fundamental aspects of DNA storage and recent advances in this field, with special emphasis on natural processes and solutions that can be exploited. We point out new ways of efficient DNA and nucleotide storage that are inspired by nature. Within a few years DNA-based information storage may become an attractive and natural complementation to current electronic data storage systems. We discuss rapid and directed access (e.g. DNA elements such as promotors, enhancers), regulatory signals and modulation (e.g. lncRNA) as well as integrated high-density storage and processing modules (e.g. chromosomal territories). There is pragmatic DNA storage for use in biotechnology and human genetics. We examine DNA storage as an approach for synthetic biology (e.g. light-controlled nucleotide processing enzymes). The natural polymers of DNA and RNA offer much for direct storage operations (read-in, read-out, access control). The inbuilt parallelism (many molecules at many places working at the same time) is important for fast processing of information. Using biology concepts from chromosomal storage, nucleic acid processing as well as polymer material sciences such as electronical effects in enzymes, graphene, nanocellulose up to DNA macramé , DNA wires and DNA-based aptamer field effect transistors will open up new applications gradually replacing classical information storage methods in ever more areas over time (decades).