Refine
Has Fulltext
- yes (30)
Is part of the Bibliography
- yes (30) (remove)
Year of publication
- 1982 (30) (remove)
Document Type
Language
- English (30) (remove)
Keywords
- Biochemie (3)
- Chemie (2)
- Differential effects of stressors (2)
- Healthy subjects (2)
- Physiologische Chemie (2)
- Tranquillizer (2)
- 0 antigen (1)
- Activation (1)
- Amphibian oocytes (1)
- Anxiolysis (1)
- Bordeiella pertussis (1)
- Carcinogenesis (1)
- Covalent binding index - Diethylstilbestrol (1)
- DNA binching (1)
- Dextran sulphate (1)
- Electron microscopy (1)
- Estrogen (1)
- Eukaryoten (1)
- Examination (1)
- Group dynamics (1)
- Hormone (1)
- Lampbrush chromosomes (1)
- Lebendgebärende Zahnkarpfen (1)
- Macrophage (1)
- Medizin (1)
- Mouse (1)
- Neurobiologie (1)
- Organische Chemie (1)
- Physiologie (1)
- Protein-Tyrosin-Kinasen (1)
- Salmonella typhimurium (1)
- Schwertkärpfling (1)
- Social stress (1)
- Social stress situations (1)
- Telemetry (1)
- Telemetry Activation (1)
- Toxicity ; plasmids (1)
- Toxikologie (1)
- Transcription units (1)
- adenosine (1)
- gene-cloning (1)
- histamine release (1)
- human lung (1)
- mast cells (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (15)
- Institut für Molekulare Infektionsbiologie (3)
- Neurochirurgische Klinik und Poliklinik (3)
- Institut für Anorganische Chemie (2)
- Institut für Pharmakologie und Toxikologie (2)
- Institut für Organische Chemie (1)
- Institut für Psychologie (1)
- Institut für Psychologie (bis Sept. 2007) (1)
- Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) (1)
- Physikalisches Institut (1)
We have cloned the chromosomal hemolysin determinants from Escherichia coli strains belonging to the four O-serotypes 04, 06, 018, and 075, The hemolysin-producing clones were isolated from gene banks of these strains which were constructed by inserting partial Sau3A fragments of chromosomal DNA into the cosmid pJC74. The hemolytic cosmid clones were relatively stable. The inserts were further sub cloned either as Sail fragments in pACYC184 or as BamHI-SaLI fragments in a recombinant plasmid (pANN202) containing cistron C (hlye) of the plasmid-encoded hemolysin determinant. Detailed restriction maps of each of these determinants were constructed, and it was found that, despite sharing overall homology, the determinants exhibited minor specific differences in their structure, These appeared to be restricted to cistron A (hlyA), which is the structural gene for hemolysin. In the gene banks of two of these hemolytic strains, we could also identify clones which carried the genetic determinants for the mannose-resistant hemagglutination antigens Vb and VIc. Both of these fimbrial antigens were expressed in the E. coli K-12 clones to an extent similar to that observed in the wild-type strains. These recombinant cosmids were rather unstable, and, in the absence of selection, segregated at a high frequency.
The role of macrophages in primary and secondary infection of mice with Salmonella typhimurium
(1982)
Elimination of macrophages with high-molecular dextran sulphate (OS) markedly impairs resistance of mice to primary infection with smooth, virulent strains of Salmonella typhimurium, whereas stimulation of this system by killed Bordetella pertussis organisms increases resistance. In infection with rough, avirulent strains of S. iyphimurium the elimination of macro phages was not followed by an essential loss of resistance, and it appears that other non-specific defence mechanisms, for example the complement system, may have compensated for the lack of macrophages. Macrophages, therefore, play an important role in defence during primary infection with virulent strains. In immunity to challenge infection with S. typhimurium, macrophages play an even more significant role. Treatment with OS completely removes immunity, and both humoral and cell-mediated immune mechanisms seem to require the participation of macrophages.
While clear evidence exists for the direct involvement of cytolysins in the pathogenesis of Gram-positive bacteria, the significance of Gram-negative haemolysins remains unclear. This paper presents briefly data indicating a role for haemolysin production in infections caused by Escherichia coli and also experiments which have allowed an analysis of the molecular basis of the haemolysis among pathogenic and non-pathogenic strains of this species.
In an experimental analog of verbal examinations, the call-up situation, the effects of two dosages of a tranquillizing agent (lopirazepam) are compared to placebo treatment. 72 male and female, healthy, young volunteers have been randomly assigned to 12 groups of 6 subjects each. Pulse frequency and performance were registered. The results indicated differential drug effects which were interpreted according to the hypotheses of "differential effects of social stressors". If a situation was highly challenging for a subject, the application of a tranquillizer in an adequately high dosage enabled him to perform well in spite of or because of strong increases in pulse frequency.
In an experimental analog of verbal examinations, the call-up situation, the effects of two dosages of a tranquillizing agent (lopirazepam) are compared to placebo treatment. 72 male and female, healthy, young volunteers have been randomly assigned to 12 groups of 6 subjects each. Pulse frequency and performance were registered. The results indicated differential drug effects which were interpreted according to the hypotheses of "differential effects of social stressors". If a situation was highly challenging for a subject, the application of a tranquillizer in an adequately high dosage enabled him to perform well in spite of or because of strong increases in pulse frequency.