Filtern
Volltext vorhanden
- ja (36)
Gehört zur Bibliographie
- ja (36)
Erscheinungsjahr
Dokumenttyp
Sprache
- Englisch (36)
Schlagworte
- cancer (10)
- oncolytic virus (6)
- oncolysis (5)
- vaccinia virus (5)
- Cancer (3)
- Nude-mice (3)
- Therapy (3)
- angiogenesis (3)
- breast-tumors (3)
- canine cancer therapy (3)
Institut
- Lehrstuhl für Biochemie (27)
- Rudolf-Virchow-Zentrum (13)
- Institut für Molekulare Infektionsbiologie (12)
- Theodor-Boveri-Institut für Biowissenschaften (7)
- Physikalisches Institut (3)
- Institut für Medizinische Strahlenkunde und Zellforschung (1)
- Julius-von-Sachs-Institut für Biowissenschaften (1)
- Klinik und Poliklinik für Anästhesiologie (ab 2004) (1)
- Medizinische Klinik und Poliklinik I (1)
Background:
Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo.
Methods:
In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection.
Results:
We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection.
Conclusions:
Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.