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Has Fulltext

  • yes (13)

Is part of the Bibliography

  • yes (13)

Year of publication

  • 1994 (7)
  • 1993 (2)
  • 1992 (4)

Document Type

  • Journal article (9)
  • Conference Proceeding (4)

Language

  • English (9)
  • German (4)

Keywords

  • Chirurgie (3)
  • Langerhans-Inseln (3)
  • Bauchspeicheldrüse (2)
  • Schwein (2)
  • Transplantation (2)
  • Heterotransplantation (1)
  • Histologie (1)
  • Isolierung <Mikrobiologie> (1)
  • Kollagenase (1)
  • enzyme activation (1)
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Author

  • Heiser, A. (13)
  • Müller-Ruchholtz, W. (13)
  • Ulrichs, Karin (13)
  • Eckstein, V. (3)
  • Bosse, M. (2)
  • Winoto-Morbach, S. (2)
  • Gier, C. (1)
  • Jäger, H. (1)
  • Krout, OS (1)
  • Vanucchi, A. (1)
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Institute

  • Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) (13)

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Erste Kieler Erfahrungen mit 45 Isolierungen von Langerhansinseln aus Schweinepankreas (1992)
Heiser, A. ; Ulrichs, Karin ; Winoto-Morbach, S. ; Vanucchi, A. ; Jäger, H. ; Müller-Ruchholtz, W.
No abstract available.
Influence of porcine strain, age, and pH of the isolation medium on porcine pancreatic islet isolation success (1994)
Heiser, A. ; Ulrichs, Karin ; Müller-Ruchholtz, W.
No abstract available
Histologic analysis of the porcine pancreas to improve islet yield and integrity after collagenase digestion (1994)
Ulrichs, Karin ; Bosse, M. ; Wacker, HH ; Heiser, A. ; Müller-Ruchholtz, W.
No abstract available
Enzyme Kinetics of Commercial Collagenases and their Influence on Porcine Islet Isolation (1994)
Heiser, A. ; Ulrichs, Karin ; Müller-Ruchholtz, W.
No abstract available
Xenogeneic Cellular Response of Human Lymphocytes Against Porcine Lymphocytes and Isolated Pancreatic Islets (1992)
Heiser, A. ; Ulrichs, Karin ; Eckstein, V. ; Müller-Ruchholtz, W.
no abstract available
Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields (1994)
Heiser, A. ; Ulrichs, Karin ; Müller-Ruchholtz, W.
Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.
The Human Cell Mediated Response to Xenogeneic (Porcine) Transplantation Antigen Depends on the Stimulator Cell Compartment (1992)
Heiser, A. ; Ulrichs, Karin ; Eckstein, V. ; Müller-Ruchholtz, W.
No abstract available
Enzyme Activities in Commercial Collagenase Preparations and Their Kinetics During Islet Isolation Procedure (1994)
Heiser, A. ; Ulrichs, Karin ; Müller-Ruchholtz, W.
No abstract available
Isolation of Islets of Langerhans: Improvement of the Isolation Technique Using the Pig Model. (1994)
Heiser, A. ; Müller-Ruchholtz, W. ; Ulrichs, Karin
During the last view years, interest in pancreatic islet transplantation for the cure of type I diabetes has increased markedly. A serious barrier to clinical islet transplantation is the isolation of a sufficient mass of viable and functional islets. We used a porcine islet isolation model to examine various parameters of the isolation procedure and to improve isolation technique.
Isolierung Langerhans'scher Inseln aus dem Pankreas von Schweinen: Einfluss biologischer und technischer Parameter auf die Inselausbeite und -integrität (1993)
Heiser, A. ; Ulrichs, Karin ; Gier, C. ; Müller-Ruchholtz, W.
No abstract available.
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