• Deutsch
  • Home
  • Search
  • Browse
  • Publish
  • Help
Schließen

Refine

Has Fulltext

  • yes (1)

Is part of the Bibliography

  • yes (1)

Year of publication

  • 2012 (1)

Document Type

  • Journal article (1)

Language

  • English (1)

Keywords

  • Biologie (1)

Author

  • Beitzinger, Christoph (1)
  • Benz, Roland (1)
  • Flatau, Gilles (1)
  • Kronhardt, Angelika (1)
  • Lemichez, Emanuel (1)
  • Rolando, Monica (1)
  • Stefani, Caroline (1)

Institute

  • Rudolf-Virchow-Zentrum (1)

1 search hit

  • 1 to 1
  • BibTeX
  • CSV
  • RIS
  • XML
  • 10
  • 20
  • 50
  • 100
Role of N-Terminal His6-Tags in Binding and Efficient Translocation of Polypeptides into Cells Using Anthrax Protective Antigen (PA) (2012)
Beitzinger, Christoph ; Stefani, Caroline ; Kronhardt, Angelika ; Rolando, Monica ; Flatau, Gilles ; Lemichez, Emanuel ; Benz, Roland
It is of interest to define bacterial toxin biochemical properties to use them as molecular-syringe devices in order to deliver enzymatic activities into host cells. Binary toxins of the AB7/8-type are among the most potent and specialized bacterial protein toxins. The B subunits oligomerize to form a pore that binds with high affinity host cell receptors and the enzymatic A subunit. This allows the endocytosis of the complex and subsequent injection of the A subunit into the cytosol of the host cells. Here we report that the addition of an N-terminal His6-tag to different proteins increased their binding affinity to the protective antigen (PA) PA63-channels, irrespective if they are related (C2I) or unrelated (gpJ, EDIN) to the AB7/8-family of toxins. His6-EDIN exhibited voltage-dependent increase of the stability constant for binding by a factor of about 25 when the trans-side corresponding to the cell interior was set to 270 mV. Surprisingly, the C. botulinum toxin C2II-channel did not share this feature of PA63. Cell-based experiments demonstrated that addition of an N-terminal His6-tag promoted also intoxication of endothelial cells by C2I or EDIN via PA63. Our results revealed that addition of His6-tags to several factors increase their binding properties to PA63 and enhance the property to intoxicate cells.
  • 1 to 1

DINI-Zertifikat     OPUS4 Logo

  • Contact
  • |
  • Imprint
  • |
  • Sitemap