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Significant Differences in Host-Pathogen Interactions Between Murine and Human Whole Blood
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-222575
- Murine infection models are widely used to study systemic candidiasis caused by C. albicans. Whole-blood models can help to elucidate host-pathogens interactions and have been used for several Candida species in human blood. We adapted the human whole-blood model to murine blood. Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. This coincided with less fungal association with leukocytes, especially neutrophils. The lower neutrophil number in murine blood onlyMurine infection models are widely used to study systemic candidiasis caused by C. albicans. Whole-blood models can help to elucidate host-pathogens interactions and have been used for several Candida species in human blood. We adapted the human whole-blood model to murine blood. Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. This coincided with less fungal association with leukocytes, especially neutrophils. The lower neutrophil number in murine blood only partially explains insufficient infection and filamentation control, as spiking with murine neutrophils had only limited effects on fungal killing. Furthermore, increased fungal survival is not mediated by enhanced filamentation, as a filament-deficient mutant was likewise not eliminated. We also observed host-dependent differences for interaction of platelets with C. albicans, showing enhanced platelet aggregation, adhesion and activation in murine blood. For human blood, opsonization was shown to decrease platelet interaction suggesting that complement factors interfere with fungus-to-platelet binding. Our results reveal substantial differences between murine and human whole-blood models infected with C. albicans and thereby demonstrate limitations in the translatability of this ex vivo model between hosts.…
Autor(en): | Silke Machata, Sravya Sreekantapuram, Kerstin Hünniger, Oliver Kurzai, Christine Dunker, Katja Schubert, Wibke Krüger, Bianca Schulze-Richter, Cornelia Speth, Günter Rambach, Ilse D. Jacobsen |
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URN: | urn:nbn:de:bvb:20-opus-222575 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Hygiene und Mikrobiologie |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Frontiers in Immunology |
ISSN: | 1664-3224 |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 11 |
Aufsatznummer: | 565869 |
Originalveröffentlichung / Quelle: | Frontiers in Immunology 2021, 11:565869. DOI: 10.3389/fimmu.2020.565869 |
DOI: | https://doi.org/10.3389/fimmu.2020.565869 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | host-pathogen interaction; mice; neutrophils; whole blood ex vivo model |
Datum der Freischaltung: | 01.02.2022 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |